Bch 5045 Graduate Survey Of Biochemistry-PDF Free Download

BCH 5045 Graduate Survey of Biochemistry
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David L Nelson and Michael M Cox, PRINCIPLES OF BIOCHEMISTRY. Fifth Edition, CHAPTER 26, RNA Metabolism, 2008 W H Freeman and Company. Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. DNA footprint analysis, of the RNA polymerase, binding site on at the. Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. E coli RNA polymerase mediated, transcription initiation and. elongation process The, polymerase binds to the promoter.
and forms a closed complex in, which the DNA is not unwound A. 12 to 15 bp region of DNA at, about the 10 region to 2 or 3. from the transcription start site is, then unwound to form an open. complex The sigma subunit, releases and the polymerase. advances and becomes committed, to RNA synthesis and elongation.
Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. E coli RNA polymerase with DNA and emerging RNA, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Sigma subunit cycle, showing that RNA, polymerase is guided by a. bound sigma subunit and, binds to DNA at a specific. promoter sequence Once, RNA synthesis begins the, sigma subunit dissociates. and is replaced by NusA, What does NusA do, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by.
of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Seven Sigma Subunits of E coli, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. RNA polymerase pauses at certain, DNA sequences some that are. terminators The complex can, undergo a conformational change. isomerization The intramolecular, pairing of complementary. sequences in a newly formed RNA, transcript may form a hairpin that.
alters the RNA DNA hybrid and the, interactions between RNA and. polymerase resulting in an A U, hybrid region at the 3 end of the. new transcript which is relatively, unstable which causes RNA. dissociation leading to termination, of RNA synthesis. Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. This slide is not in the lecture video, Here is something to consider from recent research.
The rate of replication is much faster about 20 times than that of a. transcription complex moving along the closed circular DNA of a. bacterial chromosome or genome Therefore in bacteria a potential. for co directional going the same way collisions between the. replisome and RNA polymerase complex and head on collisions exists. The RNA polymerase complex can and does slide back and forth along. RNA and DNA at frequent sites Sliding backwards is known as. backtracking or stopped arrested transcription can happen when. ribosomes are not co translating the emerging mRNA from the. polymerase complex Co directional collisions with backtracked or. arrested transcription elongation complexes and replisomes can result. in DNA double strand breaks while head on collisions between the. two complexes seem not to result in single or double strand breaks. Dutta et al 2011 Cell 146 533 543, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Sequences in promoters recognized by eukaryotic RNA. polymerase II, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. RNAP II is a 550 kDa, complex of 12, subunits and is. perhaps the most, studied type of RNA, polymerase A range. of transcription, factors are required, for it to bind to a.
promoter and begin, transcription, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Actinomycin D, and acridine are, inhibitors of, transcription. Actinomycin D, binds DNA at the, transcription, initiation. complex and, elongation by, RNA polymerase, Space fill models of actinomycin D face on and acridine side on from. the bottom are shown, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by.
of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. processing in, eukaryotes, Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors Commentary by the instructor is protected by. of W H Freeman and Company Living Graphs software c 2008 Sumanas Inc ALL RIGHTS RESERVED Copyright c 2011 ALL RIGHTS RESERVED. Images from the Text are protected by Copyright c 2008 by W H Freeman and Company and by the licensors of W H Freeman and Company Living Graphs software c

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