Countess 3 Automated Cell Counter - Thermo Fisher Scientific
Countess 3 Automated Cell CounterUSER GUIDECatalog Number AMQAX2000Publication Number MAN0019566Revision B.0For Research Use Only. Not for use in diagnostic procedures.
Life Technologies Corporation 22025 20th Ave SE Ste. 100 Bothell, WA 98021For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.The information in this guide is subject to change without notice.DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BELIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH ORARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.Revision history: Pub. No. MAN0019566RevisionDateB.018 January 2021A.009 November 2020DescriptionUpdate Technical specificationsNew user guide.Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of theseproducts, you accept the terms and conditions of all applicable Limited Use Label Licenses.TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 2021 Thermo Fisher Scientific Inc. All rights reserved.
Contents CHAPTER 1 Product information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Upon receiving the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Register your instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Exterior instrument parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 CHAPTER 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Operating environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Install the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Turn ON the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Sign in to the instrument with a new Connect account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Sign in to the instrument with an existing Connect account . . . . . . . . . . . . . . . . . . . . . . . . . . 11 CHAPTER 3 Templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Templates screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Template functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Count parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Load a template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14Create a template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14Edit a template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14Import a template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Export a template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 CHAPTER 4 Slide preparation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Load Countess Cell Counting Chamber Slide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Load the Countess Reusable Slide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20Countess 3 Automated Cell Counter User Guide3
Contents CHAPTER 5 Cell count and cell viability assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Count cells in brightfield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Capture and count . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Next steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23View results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24Results screen for brightfield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24Identify objects counted . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24Results screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24Identify cells counted in cell count and cell viability assays . . . . . . . . . . . . . . . . . . . . . . 25Histogram count results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26View histogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26Gate count results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26Gating screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26Gate count results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27Save as new template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27Edit and save a new template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 CHAPTER 6 Calculators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Pre-Dilution Calculator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Cell Splitting Calculator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 CHAPTER 7 Save results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31Save screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31Save procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34Report file . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CHAPTER 8 Settings overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Settings screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Connect to a wireless network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Guidelines for software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Update the Countess 3/3 FL Cell Counter software . . . . . . . . . . . . . . . . . . . . . . . . . . . 36Set the date and time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36Change your cloud region . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 384Countess 3 Automated Cell Counter User Guide
Contents CHAPTER 9 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39Instrument care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39General guidelines for care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39Clean the cell counter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Clean the touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Clean the instrument case . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Decontaminate the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4040404041 APPENDIX A Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 APPENDIX B Technical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45Physical characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45Technical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45Analysis slide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 APPENDIX C Countess 3 Automated Cell Counter and accessories . . . . . . . 47Accessory products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 APPENDIX D CSV file format definition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 APPENDIX E Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51Chemical safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52Biological hazard safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 APPENDIX F Documentation and support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Customer and technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Limited product warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Countess 3 Automated Cell Counter User Guide5
1Product informationIMPORTANT! Before using this product, read and understand the information in the “Safety” appendixin this document.ContentsThe Countess 3 Automated Cell Counter is shipped with the components listed below.ComponentQuantityCountess 3 Automated Cell Counter1 eachPower cord with 4 adapters (for U.S./Canada/Taiwan/Japan, Europe, or UK)1 eachCountess Cell Counting Chamber Slides (50 slides/box)1 boxCountess 3 Disposable Slide Holder1 eachCountess 3 Reusable Slide Holder1 eachCountess 3 USB Drive1 eachCountess 3 Automated Cell Counter Quick Reference Card1 eachProduct descriptionThe Countess 3 Automated Cell Counter is a fully automated cell counter and assay platformthat state-of-the-art optics and image analysis algorithms to analyze trypan blue stained samples insuspension. The Countess 3 Automated Cell Counter offers an intuitive user interface with the option to savedata and generate a report, which can then be transferred to a PC using the USB drive suppliedwith the instrument or available separately. The cells to be counted are loaded into the instrument in disposable Countess Cell CountingChamber Slides. Each chamber slide contains two enclosed chambers to hold the sample andallow measurement of two different samples or perform replicates of the same sample. The instrument takes 10-20 seconds per sample for a typical cell count in the brightfield channeland is compatible with a wide variety of eukaryotic cells. In addition to cell count and viability, theCountess 3 Automated Cell Counter also provides information on cell size.6Countess 3 Automated Cell Counter User Guide
Chapter 1 Product informationUpon receiving the instrument1Upon receiving the instrumentExamine the instrument carefully for damage incurred during transit. Ensure that all parts of theinstrument, including accessories listed above, are included with the product. Damage claims mustbe filed with the carrier; the warranty does not cover in-transit damage.See “Install the instrument” on page 8 for instructions on installing the instrument.Register your instrumentVisit www.thermofisher.com/registercountess to register your instrument. You will be asked tosupply the serial number, your name, and your contact details. Registering your instrument ensuresthat you will receive notifications of software upgrades and information on new assays for use with theCountess cell counter.Exterior instrument parts123546123456Touchscreen display: The 7-inch capacitive display is the main user interface of the Countess 3 Automated CellCounter. It contains the buttons for all instrument functions and displays data from the cell count.Front USB port: Allows you to transfer and save the cell count data and image to an external computer for recordkeeping and printing purposes. You can use any standard FAT32-, exFAT- or NTFS-formatted USB drive for datatransfer. If desired, you can plug in a USB mouse into the rear USB port for instrument control.Slide port: Used to insert the analysis slide containing the sample into the counter.Power switch: ON/OFF rocker switch is the main power switch. It is not necessary to use the power switch forday-to-day operation of the instrument.Rear USB port: An additional USB port to perform functions such as plugging in a USB mouse for instrumentcontrol.Power input jack: Connects the instrument to an electrical outlet through the supplied power cord and theappropriate plug, based on the electrical outlet configuration in your country.Countess 3 Automated Cell Counter User Guide7
2InstallationOperating environment Place the instrument on a level surface away from vibrations emanating from other pieces ofequipment. Allow at least 5 cm (2 in) free space at the back of the instrument to allow for proper ventilation andprevent overheating of electronic components. Set up the instrument away from direct light sources, such as windows. Ambient room lighting canenter the imaging path and affect the image quality. Operating temperature range: 4 –32 C (40 –90 F). Relative humidity range: 80%.IMPORTANT! Do not position the instrument so that it is difficult to turn off the main power switchlocated on the back of the instrument.In case of an instrument malfunction, turn the main power switch to the OFF position and disconnectthe instrument from the wall outlet.Install the instrument1. Unpack the instrument and place the instrument on a flat, level, dry surface.2. Remove the thin plastic protector film from the touch-screen display.3. Plug one end of the power cord appropriate for your region into the instrument.4. Plug the power cord into the electrical outlet. Be sure to use only the power cord suppliedwith your instrument. Powering the instrument with an unapproved power cord may damage theinstrument.Turn ON the instrumentAfter unboxing, upon switching on the instrument for the first time, it will run through a series of set-upscreens. Read the End User License Agreement and press Agree to proceed with start up. Set the Date and Time using the buttons (see “Set the date and time” on page 36). Press Next. Upon completion, press Done. You will proceed to the Home screen and can begin using theinstrument as described below.8Countess 3 Automated Cell Counter User Guide
Chapter 2 InstallationSign in to the instrument with a new Connect account21. Turn on the instrument by flipping the power switch on the back of the instrument to the ONposition. See “Exterior instrument parts” on page 7.The instrument will walk you through an initial Out-of-Box Experience to set up the instrument forfirst use.2. From the Home screen, you can proceed immediately to the assays by inserting a slide. SeeChapter 5, “Cell count and cell viability assays”.Alternatively, you can change or add a template in step 3 below or change instrument settings instep 4 below.3. To change the current template or add a new template to the instrument, pressTemplates.Templates allows you to create customized count preferences (i.e., gate counts based on cell size,brightness, and/or circularity). See “Load a template” on page 14.4. To change instrument settings, pressSettings.Settings allows you to update the Countess 3 instrument software, customize instrument settings,view instrument details, and export error logs. See “Settings screen” on page 35.Sign in to the instrument with a new Connect accountNote: A Wi-Fi adapter needs to be installed and a Thermo Fisher Connect account is required forlinking. See http://thermofisher.com/connect for details on using Connect.1. PressSign in from the Home screen.2. Press Link account.Countess 3 Automated Cell Counter User Guide9
2Chapter 2 InstallationSign in to the instrument with a new Connect account3. Choose a method to connect. Press Mobile device. Scan the QR code on your instrument using the Instrument Connectapp on your mobile device. Follow the steps shown on the page. Press PC to obtain a linking code to enter online in your Thermo Fisher Connect account.4. A completion screen will show. Press Done. You then return to the Home screen and are loggedinto your account.Once set up, you will then only need to sign in to your instrument account using your Username andPIN.10Countess 3 Automated Cell Counter User Guide
Chapter 2 InstallationSign in to the instrument with an existing Connect account2Sign in to the instrument with an ex
3 Automated Cell Counter is a fully automated cell counter and assay platform that state-of-the-art optics and image analysis algorithms to analyze trypan blue stained samples in suspension. The Countess 3 Automated Cell Counter o
The guides listed below are available with the Countess II or the Countess II FL Automated Cell Counter. Guide Pub. no. Countess II and Countess II FL Automated Cell Counters User Guide : MAN0010644 . Countess II and Countess II FL Automated Cell Counters Quick Reference Card (QRC) MAN0010826
4 Overview The Countess II (cat. no. AMQAX1000) is an automated benchtop cell counter that performs cell count and viability measurements of trypan blue stained samples that are loaded into plastic, disposable countess cell counting chamber Slides. The Countess II FL (cat. no. AMQAf1000) is a fully automated cell counter that uses eVOS light cube technology to analyze fluorescently .
Cell Counting Chamber Slides (50 slides/box) 1 box Countess 3 FL Disposable Slide Holder 1 each Countess 3 FL Reusable Slide Holder 1 each Countess 3 FL Light Cube Removal Tool 1 each Countess 3 USB Drive 1 each Countess 3 FL Automated Cell Counter Quick Referenc
The Countess II Automated Cell Counter is a fully automated cell counter and assay platform that uses state-of-the-art optics and image analysis algorithms to analyze trypan blue-stained cells in suspension. The Countess II Automated Cell Counter offers an intuitive user interface, and
The Countess automated cell counter uses state-of-the-art optics and image analysis to automate cell counting. The Countess automated cell counter is a benchtop counter designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue technique.
The Countess automated cell counter uses state-of-the-art optics and image analysis to automate cell counting. The Countess automated cell counter is a benchtop counter designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue technique.
imaging systems, Invitrogen Countess II FL Automated Cell Counter, and the Invitrogen Attune Flow Cytometer. Countess II Automated Cell Counters We offer two high performance automated cell counters designed to meet the needs of any lab. The Invitrogen Countess II and Countess II FL Automated Cell Counters contain advanced .
Trypan Blue Stain (0.4%) for use with the Countess Automated Cell Counter . T10282 : Countess II Automated Cell Counter† AMQAX1000 . Countess II Automated Cell Counting Chamber Slides† C10228 : Live/DEAD Viability/Cytotoxicity Kit for mammalian cells . L3224 . EMD Millipore : Scepter 2.0 Handheld Automated Cell Counter† PHCC20060 .
