SFS, GURUKUL MARG, MANSAROVAR, JAIPUR

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International College For Girls (Autonomous)SFS, GURUKUL MARG, MANSAROVAR, JAIPURMASTER OF SCIENCE (M.Sc.)ZOOLOGYFirst Semester – Fourth Semester(2-year programme)I Semester Examination November 2007II Semester Examination April 2008III Semester Examination November 2008IV Semester Examination April 2009Syllabus applicable for the students seeking admission to the M.Sc.Zoology in theacademic year 2007-08Department of Zoology1

International College For Girls (Autonomous)SEMESTER IPAPER-I Biosystematics, Taxonomy & EvolutionPaper Code: ZOL 121MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSUNITIUNITIITOPICS UNITIII Definition and basic concepts of biosystematics andtaxonomyImportance and applications of biosytematics in biologyMaterial basis of biosystematics-different attributesTrends in biosystematicsChemotaxonomyCytotaxonomyMolecular taxonomyDimensions of speciation and taxonomic charactersDimensions of speciation – types of lineage changes,production of additional lineageMechanisms of speciation in panmictic and apomicticspeciesSpecies concept- Species category, different speciesconcept, subspecies and other infra specific categoriesTheories of Biological classification , Hierarchy ofcategoriesTaxonomic characters – different kinds, origin ofreproductive isolationProcedure keys in taxonomyTaxonomy procedures-Taxonomic collections,preservations, curetting process of identificationTaxonomic keys-Different kinds of Taxonomic keys, theirmerits and demeritsSystematic publication-Different kinds of publicationsProcess of typification and different zoological typesInternational code of Zoological nomenclature(ICZN) – itsoperative principles, interpretation and application ofimportant rules, Zoological nomenclature, formation ofscientific names of various taxa.Evaluation of biodiversity indices:Shannon-Weinner index, dominance indexTEACHINGHOURS101515Department of Zoology2

International College For Girls (Autonomous)UNITIVUNITVConcept of evolution and theories of organic evaluationwith an emphasis on Darwinism. Micro and Macro-evolution Neo-Darwinism Hardy –Weinberg law of genetic equilibrium A detailed account of Destabilizing forces:(i) Natural selection(ii) Mutation(iii) Genetic drift(iv) Migration(v) Meiotic drive Genetics of quantitative traits in populationsAnalysis of quantitative traitsQuantitative traits and natural selectionGenotype-environment interactionsInbreeding depression and heterosisMolecular analysis of quantitative traitsPhenotypic plasticityMolecular phylogeneticsHow to construct phylogenetic treesPhylogenetic inference-Distance methods, parsimonymethods, maximum likelihood methodImmunological techniquesTotal Teaching Hours101060Suggested Readings: Principal of animal taxonomy; G.G Simpson; Oxford IBH Publication Co. Elements of taxonomy; E.Mayer; Molecular markers:Natural history and evolution;J.C. Avise.Chapmann andHall.New york. Evolution;Strickberger.Jones and Barlett publisher.Boston London Threatened animals of India;B.K. Takadar.ZSI Publication .Calcutta. Biodiversity;Wilson.Academic press .WashingtonDepartment of Zoology3

International College For Girls (Autonomous)Paper II: Cell BiologyPaper Code: ZOL-122MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSTOPICSUNITICell TheoryStructure of prokaryotic and eukaryotic cells.Bio-membranes Molecular composition and arrangement functionalconsequences Transport across cell membrane: diffusion ,active transportand pumps, uniports, symports and antiports Membrane potential Co-transport by symporters or antiportersCytoskeleton Microfilaments, intermediate filaments and microtubulesstructure and dynamics Cell movements-Intracellular transport,role of kinesin and dyneinCell –Cell Signaling and Cell Interaction Introduction Cell surface receptors Second messenger system MDP kinase pathways Signaling from plasma membrane to nucleusCell junctionsCell adhesionCell cycle Cyclin and cyclin dependent kinases Regulation of CDK – cyclin activityChromosomes, Chromatin, and the Nucleosomes Chromosome sequence and diversity NucleosomesIntracellular protein traffic Protein synthesis on free and bound polysomes Uptake into ER Membrane proteins, Golgi sorting, Post translationalmodificationsApoptosis Definition ,mechanism and significanceTotal Teaching 60Department of Zoology4

International College For Girls (Autonomous)Suggested Readings: Cell Biology; Powar, C.B.; Himalaya Publications. Molecular Biology of the gene; Watson J.D; Benzamin/ Cummings Biochemistry; Voet &Voet ; John Wiley & Sons Molecuar Biology of the cell;Alberts,Bray,Lewis Gene VI;Benjamin Lewin,Oxford University Press Molecular Biology and biotechnology;A comprehensive desk reference.R.A.Mayers.VCH Publisher.Inc. Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M.Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore,Scientific American Books,USA,1994.Cell & Molecular Biology, (8th edition), E D P Roberties & E M F Roberties,Lippincott Williams & Wilkins, 2005The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of IndiaPvt. Ltd., 1990Cell and Molecular Biology, (3rd edition), P Scheeler and D E Bianchi, JohnWiley & Sons, Inc, 1987Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp,John Wiley & sons, Inc., 2005The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman,ASM Press, 2007Department of Zoology5

International College For Girls (Autonomous)PAPER III: Structure and Function of InvertebratesPaper Code: ZOL-123MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSTOPICSUNITIBasis of classification (brief account)-: Symmetry, coelom, segmentation, embryogeny and level oforganizationLocomotion Flagella and ciliary movement in protozoa Hydrostatic movement in coelenterate, annelida andechinodermataUNITIINutrition and digestion Filter feeding in polychaeta, mollusca and echinodermataRespiration Organs of respiration – gills, lungs and trachea Respiratory pigments Mechanism of respirationExcretion Organs of excretion: coelom, coelomoducts, nephridia andMalphigian tubules Mechanism of excretion Excretion and osmoregulationTrends in Neural Evolution Primitive nervous system -coelenterata and echinodermata Advanced nervous system- annelida, arthropoda(crustacean and insecta)and mollusca (cephalopoda)Invertebrate larvae Larval forms of free living invertebrates Larval forms of parasites Significance of larval formsMinor ryozoa,Phoronida) Concept and significance Organization and general charactersTotal Teaching partment of Zoology6

International College For Girls (Autonomous)Suggested Readings: The Invertebrates; Hyman.L.H.; Mc Graw Hill Co New York Invertebrate structure and function. ; Barrington.E.J.W. ThomasNelson & SonsLtd.London Invertebrate Zoology,III edition.; Barnes.R.D. Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G andRajan, R.: Orient Longman, Madras Text book of Zoology;Parker T.J. Haswell.Macmillan Co. London. Animal Parasitism ;Read C.P.Printice Hall Inc.New Jersey.Department of Zoology7

International College For Girls (Autonomous)PAPER IV: BiochemistryPaper Code: ZOL-124MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSTOPICSUNITIBasic chemical concepts A study of chemical bondsBiocatalysis Classification and nomenclature of enzymes Mechanism of enzyme action Enzyme kinetics Inhibition of enzyme Isoenzymes and co-enzymesEnergy consideration Biological oxidation and reduction Fundamental reactions of biological oxidation Redox potential and electron transfer system enzymesCarbohydrates Definition , general properties, classification andconfiguration of carbohydratesMetabolism Catabolism of glycogen and glucose, details of glycolysis ,kreb cycle and cori cycle Phosphogluconate pathways(pentose phosphate pathway)Synthesis of glycogen; glycogenesis and gluconeogenesisUNITIIUNITIIIUNITIVLipids Definition, general properties and classification Structure and importance of -:1. Simple lipids- fats and wax2. Compound lipids- phospholipids, glycolipids,aminolipids and sulpholipids3. Steroids- cholesterol, bile acids, steroid hormones4. LipoproteinsLipid MetabolismInborn errors of metabolismGeneral properties and classification of proteins Amino acid structure and classification Structure of protein-Primary,Secondary,Tertiary andQuartenaryTEACHINGHOURS10141214Department of Zoology8

International College For Girls (Autonomous) UNITVRamachandran plotsGlobular and fibrous protein- motif in globular proteinsProtein sequencingProtein metabolism Amino acid degradation:Deamination reaction- Oxidativeand non oxidative deamination reaction, transaminationand transdeamination, Decarboxylation, Ornithine cycle ofurea formation , Fate of carbon skeleton of aminoacids(Metabolism of individual amino acid excluded)Total Teaching Hours1060Suggested Readings: Biochemistry; Voet &Voet ; John Wiley & Sons Outlines of Biochemistry; Conn, E.E. and Stumpf P.K. ; John Wiley, NewYork. Principles of Biochemistry; Smith; Mc Graw hill, New York. Biochemistry; Stryer, L; Freeman Publications. Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G andRajan, R.: Orient Longman, Madras Practical Biochemistry ;Wilson and Walker J.Department of Zoology9

International College For Girls (Autonomous)Paper V : PracticalPaper Code: ZOL 125MM : 100 ( C.A : 30, SEE : 70)Credits: 9Contact Hr./week : 18Contact Hr./semester : 270Invertebrates:Identification, classification & study of distinguishing features of representativesfrom various groups.Phylum Protozoa- ium(Fission & glena,Trypanosoma,Monocytsis,Plasmodium, Ceratium, Euplotes, Didinium, NoctilucaPhylum Porifera-Sycon L.S & T.S, Spicules, Spongin fibres, Leucoslenia, EuplectellaPhylum Cnidaria- Obelia,( polyp & dium,Madrepora, Alcyonium, Gorgonia,Aurelia, FaviaPhylum Helminthes- Ascaris, Taenia, PlanariaPhylum Annelida- Pontobdella, Aphrodite, Leech, Polynoe,Polygordius,Chaetopterus,Neries ,Heteroneries, Arenicola, GlossiphoniaPhylum Arthropoda- Cyclops, Peripatus, Balanus, Lepas, Palinurus, Hippa, Belostoma,Limulus, EupagurusPhylum Mollusca- Tridacna, Onchidium,Olivia, Murex, Bulla, Cardium, Arca, TurritellaPhylum Echinodermata- Echinodiscus, Holothuria, Antedon,AsteriasMinor Phyla- Bugula, Plumatella, Crestatella, Pectinella,Phoronis, DendrostomaLarval Stages: Planula, Redia, Miracidium, Sporocyst,Cercaria,Metacercaria Trochophore,Nauplius, Zoea, Mysis, Phyllosoma, Trilobite larva of Limulus, Velligar, Bipinnria,Echinopluteus, Auricularia, TornariaDissections: Cockroach: General anatomy,Reproductive system,Nervous system Lamellidens Nervous system MytilusNervous system SepiaNervous systemDepartment of Zoology AplysiaNervous system, General anatomy10

International College For Girls (Autonomous)Biosystematics, taxonomy and evolution Composition and assessment of the taxonomic diversity/biodiversity in a habitat(e.g. grassland, arid land, wet land etc.) Estimation of gene and genotypic frequencies in light of Hardy-Weinberg law basedon facial traits/thumb prints, ABO blood group data etc. Project: Preparation of models showing the status of certain taxa or species in aparticular habitat.Cell Biology Preparation of different cell types: Liver Hepatocytes , Adipocytes, Spleen cellsStudy of Polytene chromosomes in salivary glands of Chironomus larva/DrosophilaLight microscopic examination and preparation of tissue sections.Study of mitotic and meiotic chromosomes from permanent prepared slides.Preparation of slides for mitotic study.Preparation of slides for meiotic study in the testis of grasshopper.Preparation of multipolar nerve cell from the spinal cord of a mammal.Barr body preparation in buccal epithelial cells.Biochemistry Qualitative analysis of Lipid, protein and Carbohydrate samples Colorimetric/Spectrophotometric estimation of : Carbohydrate-Glucose,glycogen,cholesterol etc. Protein Phospholipids Nucleic acids: DNA,RNA Analysis of enzyme activity Study of temperature effect on enzyme actionLaboratory Books Recommended: Practical Biochemistry ;Wilson and Walker J. An Introduction to Practical Biochemistry;Plummer D.T. Tata McGraw Hill. New DelhiPractical Exercises in Cytology, Genetics,Plant Breeding andBioststistics;RaghuvanshiProjects Zoology; Tampion-DoreenHutchison’s Clinical Methods;Swash and MichaelsPractical Zoology Invertebrates , S.S. Lal, Rastogi Publications.Practical Zoology Invertebrates , P.S.Verma, Rastogi Publications. Essential techniques in cell biology : Shah-Chinoy, Anada Book DepotLaboratory manual in biology : S.C. Maheshwari , Arya Books Depot.Practical zoology : Bhatia- KohliStandard methods of Biochemical Analysis;Thimmaiah,S.R. Department of Zoology11

International College For Girls (Autonomous)PAPER-VI : SEMINARPAPER CODE: ZOL 126Credits 2Marks :50 ( CA 30%, SEE 70%)Contact Hours /Semester: 30Contact Hours /Week: 2Seminars have been incorporated in the syllabi in Semester I and II to enhance thecommunication skill of the M.Sc. students.The candidates will have to choose a topic from the syllabi for seminar preparation. Theywill be expected to submit a write up pertaining to that topic and at the end of semester, apresentation will have to be made in presence of panel of experts from different fields ofZoology.Department of Zoology12

International College For Girls (Autonomous)SEMESTER IIPAPER I : General PhysiologyPaper Code: ZOL-221MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSTOPICSUNITIDigestion Digestive enzymes and digestion of carbohydrates,proteins and lipids-intestinal absorption-co-ordinationofdigestive activities- assimilation and partitioning of foodenergyPhysiology of movement Ultrastructure of skeletal, smooth and cardiac muscle Muscle contraction Physical properties of muscleUNITIIRespiration Gases in external environment. Respiratory organs andtheir ventilation. Respiratory pigments, BMR- transport ofrespiratory gases(oxygen and carbon dioxide),nervous andchemical control of respirationCirculation Vascular pumps.Cardiac muscle properties. Heart beat andits regulation, coagulation, cardiac rhythm,Haemoglobin,breathing and its regulation in man.Bloodpressure.Respiratory and cardiovascular responses inrelation to hydrostatic pressure, temperature and highaltitudeUNITIIIOsmotic and ionic regulation Maintenance of water and electrolyte balance in aquaticand terrestrial vertebrates, air breathing vertebratesExcretion Vertebrate kidney and formation of urine-nitrogenexcretion- renal regulation of acid base balanceUNITIVInformation processing in sensory and nervous system Structural elements – electric,chemical events andinformation processing membrane,generator,receptors andaction potential,synaptic transmission, neurotransmittersTEACHINGHOURS15151010Department of Zoology13

International College For Girls (Autonomous)Sensory physiology otoreception- specialized visual functions andadaptations to habitatUNIT VChemical correlation and control Endocrine glands and hormones Hormonal control of male and female reproduction andimplantation ,parturition and lactation in mammals PheromonesTotal Teaching Hours1060Suggested readings: A Textbook of Animal Physiology; Berry,A.K.; Emkay Publisher, Delhi. Biochemistry; Stryer, L; Freeman Publications. Text Book of Medical Physiology; Guyton A.C.; Biochemistry; Voet &Voet.; John Wiley & sons. Molecular Cell Biology; Lodish, H W.H.; Freeman & Company, New York Text Book of Medical Physiology; Chatterjje, M.N and Shinde, R.; Jaypeebrothers.Department of Zoology14

International College For Girls (Autonomous)PAPER II: Tools & TechniquesPaper Code: ZOL-222MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSTOPICSUNITIPrinciple and application of : Light microscopy and micrometry Phase contrast microscopy Interference microscopy Polarized microscopy Fluorescence microscopy Transmission electron microscopy Scanning electron microscopy Confocal scanning microscopyPrinciple and application of : Ultracentrifugation :Differential and density gradient Electrophoresis:Paper ,agarose,PAGE, isoelectrofocussing points and capillary electrphoresis Chromatography:Paper,TLC,GLC,HPLC,ion-exchangeand affinity chromatography Freeze techniques:Freeze –drying,Freezesubstitution,Freeze fracture and freeze itch X-ray diffraction Lamberts – Beer’s Law ,NMR,,ESR,IR, spectrophotometery Flow cytometery/fluorescence activated cell sorterPrinciple and application of radiation techniques in biology Radiation dosimetry Radioisotopes and half life of isotopes Tracer techniques in biology Auotoradiography Cerenkov radiation Liquid scintillationPrinciples and technique of : Nucleic acid hybridization and cot curves Sequencing of nucleic acids Blotting techniques(southern,northern and western) Polymerase chain reaction Screening of genomic and complementary DNA epartment of Zoology15

International College For Girls (Autonomous)UNITV Hybridoma technologyEmbryo sexingAssisted reproductive technologiesCell culture techniques:1. Cell proliferation measurements2. Cell viability testing3. Culture media preparation and cell harvestingmethodsTotal Teaching Hours1060Suggested readings: Biochemistry; Stryer, L; Freeman Publications. Biochemistry; Voet &Voet.; John Wiley & sons. Introduction to Instrumental analysis; Robert Braun:, Mc –Graw hill,International eds. A Biology Guide to princioples and techniques.Wilson and Gouldings Animal Cell Culture ;Freshney Practical Biochemistry IV Edition :Wilson and Walker Practical Biochemistry IV Edition :Wilson and Walker Animal Cell Culture- A practical Approach;John and Masters IRL Press Principles of Genetic Engineering ;Primrose Principles and Techniques in Biochemistry and Molecular Biology, Edited byKeith Wilson and John Walker, Cambridge University Press, (6th Edition),2007 Principles and Techniques in Biochemistry and Molecular Biology, Edited byKeith Wilson and John Walker, Cambridge University Press, (6th Edition),2007. Physical Biochemistry, D Friefelder, W.H. Freeman & Company, 17th Reprint,1999.Department of Zoology16

International College For Girls (Autonomous)PAPER III: Molecular Biology and BiotechnologyPaper Code: ZOL-223MM : 100 ( C.A : 30, SEE : 70)Credits: 4Contact Hr./week : 4Contact Hr./semester : 60UNITSUNITIUNITIIUNITIIIUNITIVTOPICSDNA Replication Prokaryotic & eukaryotic DNA replication Mechanics of DNA replication Enzymes and accessory proteins involved in DNAreplication DNA Repair mechanismTranscription Prokaryotic and Eukaryotic transcription RNA polymerases General and specific transcription factorsPost transcriptional modification in RNA 5’ cap formation Transcription termination 3’ end processing and polyadenylation Splicing Nuclear export of mRNA mRNA stabilityTranslation Genetic code Prokaryotic and eukaryotic translation Translational machinery Mechanisms of Initiation, Elongation and termination Regulation of Translation Co and post translational modifications of protein Recombination and repair Holiday junction, Gene targeting, Gene disru

MASTER OF SCIENCE (M.Sc.) ZOOLOGY First Semester – Fourth Semester (2-year programme) I Semester Examination November 2007 II Semester Examination April 2008 III Semester Examination November 2008 IV Semester Examination April 2009 Syllabus applicable for the students seeking a

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