NNI Nanosensor Workshop.ppt(revised For Upload).ppt

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Nanosensors:Transitioning Nanosensors from the laboratoryto the marketplace:Challenges and Lessons learnedWunmi SadikDepartment of ChemistryState University of NewYork-BinghamtonNNI Nanosensor WorkshopSeptember 11-12th, 2014,Washington, DC

Science –to –Technology (S2T)zA vast amount of nanosensors havebeen developed, tried and testedbiosensorsz electrochemical capacitorsz batteries, fuel cells, novel membranesystems and many morezzThere are many roadblocks in bridgingthe gap between academic research andthe market place

HighlightszOperational definitionsCategory 1 nanosensorz Category 2 nanosensorzzCase studiesUltra-sensitive Portable Capillary Sensor (UPAC )z CeO2, Fe2O3, TiO2, ZnO, and fullereneszTestbeds and performance metricsz Bridging the gapzza proposal for moving forward

How do you bridge the gap betweenresearch and commercialization?Answer the two key questions ofsuccessful innovation:zzCan you make a product?Can you get anyone to buy it?

Nanosensor Classificationz7\SH 1DQRVHQVRUV 1DQRWHFKQRORJ\ HQDEOHG VHQVRUV RU VHQVRUV WKDW DUH WKHPVHOYHV QDQRVFDOH RU KDYH QDQRVFDOH PDWHULDOV RU FRPSRQHQWVz7\SH 1DQRVHQVRUV 1DQRSURSHUW\ TXDQWLILDEOH VHQVRUV WKDW DUH XVHG WR PHDVXUH QDQRVFDOH SURSHUWLHVSadik et al, Journal of EnvironmentalMonitoring, 11, 25, echnology-whitepaper-0207.pdf

Category 1 NanosensorszHundreds of research articles using nanomaterials for chemical &biosensors have been published. There are dozens of reviewsavailable which partly deal with use of nanomaterials forelectrochemical nanobiosensorsNanoparticlesz Nanowiresz Nanoneedlesz Nanosheetsz Nanotubesz NanorodszBiosensors & Bioelectronics, 24, 2749-2765, 2009.

Metal-Enhanced Electrochemical Detection(MED).RZLQR , JDUZDO 5 6DGLN 2 /DQJPXLU

UPAC BiosensorSUNY-Binghamton scientists and engineers havedeveloped a portable, fully autonomous, and remotelyoperated sensing device, called Ultra-SensitivePortable Capillary Sensor (U-PAC )ϭ͘ ĂĚŝŬ͘ K͕͘ ĂƌĂƐŝŶƐŬŝ͕ :͕ ͞hůƚƌĂͲ ĞŶƐŝƚŝǀĞ͕ WŽƌƚĂďůĞ ĂƉŝůůĂƌLJ ĞŶƐŽƌ͕͟ h͘ ͘ WĂƚĞŶƚ EŽ͘ ϴ͕ϰϭϰ͕ϴϰϰ Ϯ͕ Ɖƌŝů ϵ͕ ϮϬϭϯ͘Ϯ͘ ĂĚŝŬ͘ K͕͘ ĂƌĂƐŝŶƐŬŝ͕ :͕ ͞hůƚƌĂͲ ĞŶƐŝƚŝǀĞ͕ WŽƌƚĂďůĞ ĂƉŝůůĂƌLJ ĞŶƐŽƌ͕͟ h͘ ͘ WĂƚĞŶƚ EŽ͘ ϳ͕ϳϬϴ͕ϵϰϰ͕ DĂLJ ϱ͕ ϮϬϭϬ͘3. Sadik, O., Wang Q., Blythe, P., US Provisional Application No. 32291/1310 (RB-347), “Capillary Biosensoand its Method of Use”, April 19, 20105. Analytical Chemistry, 74,713-719, 20026. Guide 101-10, March 2007, US Department of Homeland Security, Preparedness Directorate,Office of Grants and Training Systems Support Division, Washington DC.

Performance CharacteristicsTechniqueLODUPAC 00spores/mlStandard ELISAStandard PCROptical Leaky CladwaveguidebiosensorDOXQualitativeResponseTime30 minSample Preparation6hrsExtensive12 hrs40 minExtensive (PCRextraction)Autonomous30 minMinimalMinimal

Conventional and emerging tools forcharactering engineered nanoparticlesSP-ICP-MS Single Particle Inductively Coupled Mass Spectrometer,FFF-ICP-MS Fluid Flow Fractionation Inductively Coupled Mass Spectrometer,EC-TFF Electro-Chemical Tangential Fluid Flow, DOX-EC Dissolved oxygen Sensor coupled with Electrochemical technique, DLS Dynamic Light Scattering.

Category 2:Size-exclusive Nanosensors forQuantitative Analysis of FullerenesSADIK et al, ES&T 2011, 45, 5294 – 5294A single-use quantity of cosmetic (0.5 g) may contain up to 0.6μg of C60 and demonstrates a pathway for human exposure toengineered fullerenesBenn et al., Environ. Poll. (2011)

Nanosensor ResponsesDose dependentresponsezActive sensing electrode surface area of 0.196 cm2, an equivalent of [ EHWD &'V should fit on the QCM sensorzAt low concentrations, the ratio of beta-CD/C60 molecules was a & FDYLW\ which, is consistent with the host-guest chemistry of beta-CD-C601:1 inclusion chemistryES&T 2011, 45, 5294 – 5294.

Category 2:Capture and Detection of AerosolNanoparticles using Poly (amic)acid, Phase-inverted Membranes1SUNY-BINGHAMTON,NY2 HARVARD SCHOOL OF PUBLIC HEALTH, MA, Sadik, Demokritou et al,J. Hazardous Materials, 2014(In press), Nanoletters 2014

Harvard’s VENGESNew Platform for pulmonary and cardiovascular toxicologicalcharacterization of inhaled ENMsNanotoxicology, 2011; Early Online, 1–11

Surface Characterization

Proposal for Going Forward Develop the necessary calibrationand validation toolsDevelop SRMs and the analyticalquality control toolsDevelop acceptable standardstestbeds & characterization centers

Overcoming Present Challengesz Develop acceptablez Depends on testbedsSRMsz Calibration/validationtoolsz Standardization and TestingCentersz Develop training manuals & SOPsz Define measures of success

Test beds depend on theapplicationz Healthz Foodz Pharmaceuticalz Processz Environmentalz Defense& Security

Testbed SpecificationszEnvironmental sensor should be sensitive,specific, provide fast response, must bereliable, flexible and capable of rapid anddirect detection of toxic compounds.z Additionally, there should be no need forsample preparation steps when analyzingenvironmental matrices or point-of-carebiomedical samples.z The sensor should be capable of convenientsignal processing that will allow immediateremedial actions to be taken after detection

Environmental and Clinical Requirementsz Precision,accuracy,measurement range, total errorz Interferencez Referencez Response timez Calibrationz Manufacturingz Single use Vs. multi-use

Nanosensor PerformanceMetrics-EPA QA/QCzData quality parameterszzMethod DeterminationzzMethod positive control, matrix spike, negative control(buffers,blanks, reagent water)FrequencyzzPrecision, accuracy, LOD, robustness etcWith every field sample, 1/batch or 20 samples, 10% of fieldsamples, all standards, blanks, samplesQuality objective & Comparabilityz% RSD, MDL, intended use of datazDesignated Analytical Levels.zSadik et. al, Journal of Environmental Monitoring, 6,513-522, 2004; US-EPA (1995) and revisions. TestMethods for Evaluating Solid Waste & Emergency Response, Washington DC.

Performance MetricszExperimental variables should be definedSensitivity should be definedz Selectivity and reliability (false positives andfalse negatives) should be assessed usingSOPs.z Optimization of experimental variablesinfluencing sensor selectivity and sensitivity aswell as the transfer to manufacturing platforms.zzComparable to standard EPA, AOAC or FDAmethods.

Conclusions -Needs of the Community)zManufacturing must produce stable sensors withuniform and non-distortable signals acrosssensing areaz Sensor layers must be mounted with a suitabletransducer that does not distort themz Unpreventable calibration errors in the devicesmust be reduced to an acceptable levelz Developing QC for the sensor industry requiresthe collaboration between the manufacturing,government, and research laboratories

Sadik et al, Journal of Environmental Monitoring, 11, 25, 2009. Category 1 Nanosensors zHundreds of research articles us ing nanomaterials for chemical & biosensors have been published. There are dozens of reviews available which partly deal wit

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Wunmi Sadik Department of Chemistry State University of New York-Binghamton . NNI Nanosensor Workshop . September 11-12th, 201

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