Department Of Biochemistry Mahatma Phule Krishi
Department of BiochemistryMahatma Phule Krishi VidyapeethRahuri-413 722, Dist. Ahmednagar(MS)Master’s Programme in BiochemistryCourse LayoutMinimum Credit RequirementsSr. earchTotal CreditsCompulsory Non Credit CoursesSr.CourseCourse TitleNo.NumberA) Major Subjects (Min. 21 credits)BIOCHEM 501 BASIC BIOCHEMISTRY1BIOCHEM 502 INTERMEDIARY METABOLISM2BIOCHEM 503 ENZYMOLOGY3BIOCHEM 504 MOLECULAR BIOLOGY4BIOCHEM-505 TECHNIQUES IN BIOCHEMISTRY5BIOCHEM 507 PLANT BIOCHEMISTRY6BIOCHEM 510 CARBON AND NITROGEN METABOLISM7Minimum credit(s)21090501205606Credits2 1 33 0 32 1 32 1 31 2 33 0 32 1 3Total 15 6 21B) Minor Subjects (Min. 9 credits)1MICRO 501MICROBIAL GENETICS2 1 32FST 523NEUTRACEUTICALS AND HEALTH FOODS2 1 33PP 503PHYSIOLOGICAL AND MOLECULAR RESPONSES 2 1 3
OF PLANTS TO ABIOTIC STRESSESTotalC) Supporting Subjects (Min. 5 credits)1STAT 511STATISTICALMETHODSFORSCIENCES2MBB 508GENOMICS AND PROTEOMICS6 3 9APPLIED 2 1 3D) Seminar (01 credit)BIOCHEM 591 MASTER’S SEMINAR12 0 2Total 4 1 51 0 1Total 1 0 1E) Master's Research (20 credits)BIOCHEM 599 MASTER’S RESEARCH1F) Non Credit Compulsory CoursesPGS 501LIBRARY AND INFORMATION SERVICES12PGS 5023PGS 5034PGS 5045PGS 5056PGS 5060 20 200 1 1TECHNICAL WRITING AND EMENT IN CULTURAL RESEARCH, RESEARCH ETHICSAND RURAL DEVELOPMENT PROGRAMMESDISASTER MANAGEMENT0 1 11 0 10 1 11 0 11 0 1Total 3 3 6
Course ContentsBIOCHEM 501BASIC BIOCHEMISTRY2 1Theory:UNIT IScope and importance of biochemistry in agriculture; Fundamental principlesgoverning life; structure of water; acid base concept and buffers; pH; hydrogen bonding;hydrophobic, electrostatic and van der Waals forces; General introduction to physicaltechniques for determination of structure of biopolymers.UNIT IIClassification, structure and function ofbiomembranes, amino acids, proteins, and nucleic acids.carbohydrates,lipidsandUNIT IIIStructure and biological functions of vitamins, enzymes classification andmechanism of action; regulation, factors affecting enzyme action. Hormones- animalplants and insects, Fundamentals of thermodynamic principles applicable to biologicalprocesses, Bioenergetics.UNIT IVMetabolism of carbohydrates, photosynthesis and respiration, oxidativephosphorylation, lipids, proteins and nucleic acids. DNA replication, transcription andtranslation; recombinant DNA technologyPractical:Preparation of standard and buffer solutions, Extraction and estimation ofsugars, Amino acids, Estimation of Proteins by Lowry’s method, Estimation of DNAand RNA by phenylamine and orcinol methods. Estimation of ascorbic acid, separationof biomolecules by TLC and paper chromatography.Suggested Readings:Conn EE & Stumpf PK. 1987. Outlines of Biochemistry. John Wiley.Metzler DE. 2006. Biochemistry. Vols. I, II. Wiley International.Nelson DL & Cox MM. 2004. Lehninger Principles of Biochemistry. 4th Ed.MacMillan.Voet D, Voet JG & Pratt CW. 2007. Fundamentals of Biochemistry. JohnWiley.
BIOCHEM 501BASIC BIOCHEMISTRY2 1Teaching Schedule ame of the topicsNo ofWeightageLecture (%)Scope and importance of biochemistry in agriculture13Fundamental principles governing life13Structure of water, acid-base concept13Buffer and pH, hydrogen bonding, hydrophobic,14electrostatic and van der Waals forcesFundamentals of thermodynamic principles applicable to25biological processes, bioenergeticsClassification, structure and functions of carbohydrates15Metabolism of carbohydrates26Electron transport chain and oxidative phosphorylation16Photosynthesis26Classification, structure and functions of lipids24Metabolism of lipidsClassification, structure and functions of amino acids and26proteins. Metabolism of proteinsStructure, biological functions and classification of14vitaminsEnzyme classification, factors affecting on enzyme14actionMechanism of enzyme action24Regulation of enzyme activity14Hormones: animals, plants and insects24Structure and functions of nucleic acids15Metabolism of nucleic acids25DNA replication15DNA transcription24Translation25Recombinant DNA technology15Total32100BIOCHEM 501BASIC BIOCHEMISTRY2 1Lesson Plan (Theory)LectureNo123Name of the topicsScope and importance of biochemistry in agriculture-Origin ofbiochemistry, details of the contributions of various biochemistsFundamental principles governing life-Cellular foundation, chemicalfoundation, physical foundation ,genetic, evolutionaryStructure of water-Physical and chemical properties of water, suitability of
456789101112131415161718192021222324water as a solvent for a living cell ,acid-base conceptBuffers and pH, bonds and various forces-Definition of buffer, suitabilityof buffer for various bimolecular extractions, strength and molarity ofbuffer, definition of pH, Handerson-Hasselbalch equation, hydrogenbonding, hydrophobic bond, electrostatic bond, van der Waals forces,Fundamentals of thermodynamic principles applicable to biologicalprocesses, bioenergetics, entropy, enthalphy, Gibbs equationClassification, structure and functions of carbohydratesMetabolism of carbohydrates- glycolysis, TCA cycle, pentose phosphatepathway, glyoxylate cycle, sucrose synthesis, glucose and fructosesynthesisElectron transport chain and oxidative phosphorylationPhotosynthesis-structure of chloroplasts, photosystem I and photosystemIIClassification, structure and functions of lipidsMetabolism of lipids- β-oxidation of fatty acids, synthesis of fatty acids byacyl carrier proteinClassification, structure and functions of amino acids and proteinsMetabolism of proteins: protein degradation and protein synthesisStructure, biological functions and classification of vitaminsEnzyme Classification, factors affecting enzyme activity: substrateconcentration, enzyme concentration, temperature and pHMechanism of enzyme action : Ribonuclase A, chymotrypsin, lysozymeRegulation of enzyme activity- Allosteric enzyme, covalent modification,kinetic properties of enzymes, proteolytic cleavage of enzymesHormones: animals, plants and insects-Diverse structure & functions,action through specific high affinity cellular receptors, insulin, glucagon,epinephrine.Structure and functions of nucleic acids-double helix structure of DNA,nitrogenous bases, DNA as the genetic material.Metabolism of nucleic acids-nucleotide synthesisDNA replicationDNA transcriptionTranslation of genetic informationRecombinant DNA technology-Total RNA isolation, cDNA synthesis,cloning of cDNA in a vectorBIOCHEM 501BASIC BIOCHEMISTRY2 1Lesson Plan (Practical)Sr.No.Name of the practical1.Preparation of standard and buffersolutionsEstimation of reducing sugars by NelsonSomogyi method2.No.of practicalclasses2Weightage(%)11210
3.Estimation of free amino acids byninhydrin methodEstimation of protein by Lowry methodIsolation of DNA and its quantificationEstimation of ascorbic acidIsolation of RNA and its quantificationSeparation of amino acid by TLCSeparation of amino acids by paperchromatographyTotal4.5.6.7.8.9.BIOCHEM 502112231222151512128416100INTERMEDIARY METABOLISM3 0Theory:UNIT IThe living cell a unique chemical system, Introduction to metabolism, methodsof studying metabolism, transport mechanism, bioenergetics, biological oxidation,signal transduction.UNIT IICatabolic and anabolic pathways of carbohydrates, lipids, regulation and theirmetabolic disorders. Energy transduction and oxidative phosphorylation.UNIT IIIGeneral reactions of amino acid metabolism, Degradative and biosyntheticpathways of amino acids and their metabolic disorders. Sulphur metabolism, Metabolicengineering concepts.UNIT IVCompartmentation of metabolic pathways, metabolic profiles of major organsand regulation of metabolic pathways.Suggested Readings:Berg JM, Tymoczko JL, Stryer L & Clarke ND 2000. Biochemistry. 5th Ed. WHFreemanMetzler DE. 2006. Biochemistry. Vols. I, II. John Wiley.Voet D, Voet JG & Pratt CW. 2007. Fundamentals of Biochemistry. John Wiley.Zubey GL. 1998. Biochemistry. 4th Ed. WCB London.
BIOCHEM 502Intermediary Metabolism3 0Teaching Schedule (Theory)Sr.No.1Name of the topicsNo ofWeightageLectures (%)The living cell, unique chemical system, introduction027to metabolism, methods of studying metabolism.2Transport mechanism0253Bioenergetics, biological oxidation05104Signal transduction0265Catabolic and anabolic pathways of carbohydrates,08150712regulation and their metabolic disorders.6Catabolic and anabolic pathways of lipid, regulationand their metabolic disorders7Energy transduction and oxidative phosphorylation05108General reactions of amino acid metabolism0259Degradative and biosynthetic pathways of amino acid0510their metabolic disorders.10Sulphur metabolism02511Metabolic engineering concepts02512Compartmentation of metabolic pathways02513Metabolic profiles of major organs and regulation of04548100metabolic pathways.TotalBIOCHEM 502Intermediary Metabolism3 0Lesson Plan (Theory)No. ofLecture12345Name of the topicsThe living cell, unique chemical systemIntroduction to metabolism: catabolism and anabolism, methods of studyingmetabolism-types, regulationTransport mechanism: Introduction to membrane, membrane permeability,passive transport.Active transport: ATP-driven active transport, action of Na /K -ATPase,ion driven transport, glucose transport into intestinal epithelial cells,glucose rehydration therapy, exocytosis, endocytosis, picocytosis, receptormediated- endocytosis.Bioenergetics: General concepts of thermodynamics, free energy, enthalpy
23334-3536373839-40and entropyExergonic and endergonic reaction, their coupling.Equilibrium constant G, G0High-energy compoundsSignal transduction: cell signaling hormonesCell- surface receptors, secondary messengersCarbohydrate metabolism: Glycolysis, fates of pyruvate, inhibitors ofglycolysis and regulation of glycolysisTCA cycle and its regulationGluconeogenesis, glyoxylate cycle,Glycogen metabolism, glycogenesis, glucogenolysisStarch synthesis, sucrose synthesis, interconversion of starch and sucroseand its regulationHMP shunt, Entner-Doudoroff pathwayRegulation of carbohydrate metabolismLipid metabolism: Fatty acid oxidation: β-oxidation in mitochondria,oxidation of palmitate, oxidation of odd chain of fatty acidsOxidation of unsaturated fatty acids (one double bond and two doublebond)β-oxidation of fatty acids in peroxisomes, regulation of fatty acid oxidationα-and ω-oxidation of fatty acids, formation of ketone bodiesBiosynthesis of fatty acids and regulationElongation of saturated fatty acids, desaturation of fatty acid chainBiosynthesis of triacylglycerol, metabolism of phospholipids, synthesis ofspingolipids, degradation of spingomyelinEnergy transduction and oxidative phosphorylation: Redox potential,electron transport from NADHFormation of an H gradient, electron transport from FADH2, electrontransport inhibitorsOxidative phosphorylation, ATP synthesis as a rotatory engineCoupling and respiratory control,Uncouplers, reoxidation of cytosolic NADHOverview of catabolism of amino acids, urea cycle, metabolic fates ofamino groupsGeneral aspects of amino acid metabolism (transamination anddeamination)Pathways for amino acid degradation: ten amino acids degraded to acylCoA five amino acids are converted to α-ketoglutarate, four amino acids areconverted to succinyl CoA, two amino acids are degraded to oxaloacetateBiosynthesis of amino acids: α-ketoglutarate to glutamine, glutamate,proline and arginine, 3-phosphoglycarate to glycine, serine and cysteineThree non-essential and six essential amino acids are synthesized fromoxaloacetate and pyruvate.Three amino acids synthesis from PEP and erythrose 4-phosphate, histidinebiosynthesis, regulation of amino acids synthesisSulphur metabolism: sulphur assimilation, transport and reduction, sulphurcycle
414243444546-4748Modulation of specific metabolic pathways,Gene silencing by antisense RNA technologyGene activation for enhanced expression coding for important regulatoryenzymesCompartmentation of metabolic pathways: Metabolic pathways inperoxisomes and glyoxysomes, metabolic pathways operating inmitochondriaCompartmentation of glycolytic and glycolgenolytic metabolism.Metabolic profiles of major organs and regulation of metabolic pathways:Organ specialization and metabolic integration (liver, adipose tissue,skeletal muscles, brain, blood )Regulation of metabolismBIOCHEM 503ENZYMOLOGY2 1Theory:UNIT IIntroduction and historic perspective, Enzyme nomenclature and classification,enzyme compartmentalization in cell organelles, isolation and purification of enzymes,measurement of enzyme activity. ribozymes, isozymes, abzymes,UNIT IIEnzyme structure, enzyme specificity, active site, active site mapping,mechanism of enzyme catalysis. cofactors, coenzymes- their structure and role.UNIT IIIEnzyme kinetics, enzyme inhibition and activation, multienzyme complexes,allosteric enzymes and their kinetics, regulation of enzyme activity.UNIT IVIsolation and purification of enzymes, Applications of enzymes in chemical andfood industry, enzyme immobilization, biosensors and clinical applications of enzymes.Practical:Enzyme assay by taking any model enzyme like alpha-amylase or acidphosphatase, isolation and purification of any model enzyme like alpha amylase or acidphosphatase, study of the effect of enzyme and substrate concentrations anddetermination of Km and Vmax, determination of pH 32 and temperature optima andeffect of various inhibitors, determination of the pH and temperature stability ofenzyme.
Suggested Readings:Bergmeyer HU. 1983. Methods of Enzymatic Analysis. Vol. II. Verlag Chemie,Academic Press.Dixon M, Webb EC, Thorne CJR & Tipton KF. 1979. Enzymes. 3rd Ed. Longman.Maragoni AG. 2003. Enzyme Kinetics - A Modern Approach. John Wiley.Palmer T. 2001. Enzymes: Biochemistry, Biotechnology and Clinical Chemistry. 5thEd. Horwood Publ.Price NC & Stevens L. 2003. Fundamentals of Enzymology. Oxford Univ. Press.Wilson K & Walker J. (Eds.). 2000. Principles and Techniques of PracticalBiochemistry. 5th Ed. Cambridge Univ. Press.Thimmaiah SK, 1999. Standard Methods of Biochemical Analysis , KalyaniPublication, New Delhi.Voet D, Voet JG & Pratt CW. 2007. Fundamentals of Biochemistry. John Wiley.BIOCHEM 503ENZYMOLOGY2 1Teaching Schedule (Theory)Sr.No.123456789101112131415Name of the topicsNo ofLecture2and2History, importance and scope of talization in cell organellesBasic principles of enzyme isolation, purification andmeasurement of enzyme activitiesEnzyme structure, specificity and concept of active siteConcept of free energy, transition state, activationenergy in relation to enzymatic catalysisConcept of cofactors, prosthetic group, their structuresand functionsEnzyme kinetics: Michaleis- Menten Equation,Lineweaver- Burk plot, Vmax and its significance.Enzyme inhibition: competitive non-competitive, uncompetitive and irreversible inhibitionsMultienzyme systems and complexes and regulationof their activitiesAllosterc enzymes, kinetics and their regulationRegulation of enzyme activityMeaning of isozymes, abzymes, ribozymes and theirimportanceApplications of enzymes in agriculture, food industry,medicine and chemical industryEnzyme immobilization: methods, advantages anddisadvantagesBiosensors and its 12225425251322100
BIOCHEM 503ENZYMOLOGY2 1Lesson Plan 0212223242526272829Name of the topicsHistorical development in the field of enzymologyImportance and scope of enzymesEnzyme nomenclature and classificationCompartmentalization of enzymes in cell organellesPrinciples of enzyme isolation and purificationMeasurement of enzyme activityEnzyme structure stability and specificityConcept of active site of an enzymeOrientation of enzyme and enzyme activityBasic principles of thermodynamics and concept of free energyTransition state and activation energy in relation to enzymatic catalysisEnzyme co-factors: meaning, types and their functionsEnzyme prosthetic groups, co-enzymes: structure and functionsEnzyme kinetics: Michaleis- Menten Equation,Enzyme kinetics: Lineweaver Burk Plot, Lineweaver EquationKm, Vmax and their significanceIrreversible enzyme inhibition: meaning, type and exampleReversible enzyme inhibition: meaning, type and exampleMultienzyme systems and complexesRegulation of multienzyme activitiesAllosteric enzymes : Meaning and their kineticsRegulation of allosteric enzymesMeaning and importance of isozymes, ribozymesMeaning and importance of abzymesApplications of enzymes in food, agriculture and medicinesMeaning, importance of enzyme immobilizationApplications of immobilized enzymesMethods of enzyme immobilization, merits and demeritsBiosensors: meaning and its applicationsClinical applications of enzymes
BIOCHEM 503ENZYMOLOGY2 6Name of the topicsIsolation and estimation of amylase activity from germinating seedsEstimation of optimum temperature of amylase enzymeEstimation of optimum pH of amylase enzymeEstimation of V max of amylase enzymeEstimation of Km value for amylase enzymeIsolation and estimation of polyphenol oxidase activityIsolation and estimation of peroxidase activityIsolation and estimation of alkaline phosphatase from sugar cane juiceIsolation and estimation of acid phosphatase from sugar cane juiceIsolation and estimation of P5CS activity from stressed seedlingsBIOCHEM 504MOLECULAR BIOLOGY2 1Theory:UNIT IHistorical development of molecular biology, nucleic acids as genetic material,chemistry and structure of DNA and RNA, Genome organization in prokaryotes andeukaryotes, chromatin structure and function.UNIT IIDNA replication, DNA polymerases, topoisomerases, DNA ligase, reversetranscriptase, repetitive and non-repetitive DNA, satellite DNA; transcription process,RNA editing, RNA processing.UNIT IIIRibosomes structure and function, organization of ribosomal proteins and RNAgenes, genetic code, aminoacyl tRNA synthases’ inhibitors of replication, transcriptionand translation; translation and Post translational modification; nucleases and restrictionenzymes, regulation of gene expression in prokaryotes and eukaryotes, molecularmechanism of mutation.UNIT IVDNA sequencing, recombinant DNA technology, vectors, isolation of genes,Recombinants vector, selection of recombinants, PCR; general features of replication,transcription, site directed mutagenesis and translation in eukaryotes.
Practical:Isolation and purification of DNA and RNA from different sources, check ofpurity of isolated DNA and RNA, restriction fragmentation and separation of oligos byagarose electrophoresis, RAPD analysis of DNA, cDNA synthesis using PCR, Southernand Northern blotting experimentsSuggested Readings:Adams RLP, Knowler JT & Leader DP. 1992. The Biochemistry of the NucleicAcids. 11th Ed. Chapman & Hall.Alberts B, Bray D, Lewis J, Raff M, Roberts K & Watson JD 2006. MolecularBiology of the Cell. 6th Ed. Garland Publ.Blackburn GM & Gait MJ. 1996. Nucleic Acids in Chemistry and Biology. 2nd Ed.Oxford University Press.Freifelder D & Malacinski GM. 1996. Essentials of Molecular Biology. 3 rd Ed.Panima.Glick BR & Pasternak JJ. 1994. Molecular Biology: Principles and Applications ofRecombinant DNA Technology. ASM Press.Lewin B. 2007. Genes IX. Oxford University Press.Lodish H, Berk A, Zipursky SA, Matsudaira P, Baltimore D & Darnell J. 1999.Molecular Cell Biology. WH Freeman.Old RW & Primrose SB. 1989. Principles of Gene Manipulation: An Introduction toGenetic Engineering. 4th Ed. Blackwell Scientific Publ.Sambrook J & Russel DW. 2001. Molecular Cloning: A Laboratory Manual. Vols.I-III. Cold Spring Harbor.Voet D, Voet JG & Pratt CW. 2007. Fundamentals of Biochemistry. John Wiley.BIOCHEM 503MOLECULAR BIOLOGY2 1Teac
Lehninger Principles of Biochemistry. 4th Ed. MacMillan. Voet D, Voet JG & Pratt CW. 2007. Fundamentals of Biochemistry. JohnWiley. BIOCHEM 501 BASIC BIOCHEMISTRY 2 1 Teaching Schedule (Theory) Sr. No. Name of the topics No of Lecture Weightage (%) 1 Scope and importanc
Mahatma Jyotiba Phule : A Modern Indian Philosopher by Dr. Desh Raj Sirswal अक्टूबर 14, 2013 JOTIRAO GOVINDRAO PHULE occupies a unique position among the social reformers of Maharashtra in the nineteenth century. While other reformers concentrated more on reforming the social institutions of family and marriage with
EC_54_RAR_119 dated 8-1-2011-Savitribai Phule Pune University, Pune, Maharashtra.doc Page 1 Savitribai Phule Pune University Annual Quality Assurance Report (AQAR) of the IQAC July 1, 2013 to June 30, 2014 Part – A 1. Details of the Institution 1.1 Name of the Institution 1.2 Address Line 1 Savitribai Phule Pune University Address Line 2 City .
This is the tragedy of our Indian education system that those thinkers who really contributed in the educational, socio-political and other aspects of Indian peoples are remained far from core academic environment. This is the real need of our education system that ideas of revolutionaries like Mahatma Jyotiba Phule , Dr.
Department of Biochemistry - Website information 1. Department: Biochemistry 2. About Department The Department of Biochemistry was instituted as one of the departments of Sciences in the academic year 1998-1999. 3. Objective & Scope To enable the students to understand the concept of biochemistry regarding biomolecules
MAHATMA GANDHI MEDICAL COLLEGE & RESEARCH INSTITUTE, . POSTGRADUATE DIPLOMA IN GOOD CLINICAL LABORATORY PRACTICES 2019 -20 ONWARDS Department of Biochemistry Mahatma Gandhi Medical College and Research . of India (PHFI) as its technical partner and constituted the National Initiative for Allied Health Sciences (NIAHS) secretariat with a .
4. Plant Biochemistry 5. Clinical Biochemistry 6. Biomembranes & Cell Signaling 7. Bioenergetics 8. Research Planning & Report Writing (Eng-IV) 9. Nutritional Biochemistry 10. Bioinformatics 11. Industrial Biochemistry 12. Biotechnology 13. Immunology 14. Current Trends in Biochemistry
1. Fundamentals of Biochemistry by J.L. Jain 2. Biotechnology by B.D., Singh 3. Principles of Biochemistry by Lehninger, Nelson & Cox 4. Outlines of Biochemistry by Conn & Stumpf 5. Textbook of biochemistry by A VSS, Ramarao 6. An Introduction to Practical Biochemistry by D.T. Plummer 7. Laboratory Manual in Biochemistry by Jairaman
12 days F protect from light during storage 13 days F 1 g - 50 mL 1 g - 100 mL CEFAZOLIN7 Teva, Sandoz, Apo) NS CASPOFUNGIN1 (Cancidas ) 50 mg 10.5 mL SWFI 5 mg/mL AZTREONAM1,3 (Azactam ) 7 days F 24 hr F 30 days RT 500 mg/250 mL D5W MB D5W
