Containment Zone Entry - University Of Guelph

UNIVERSITY OF GUELPHBIOSAFETY INSPECTION CHECKLISTPrincipal Investigator:Biohazard Permit #: Containment levelLaboratory Representative(s) Present:Location (Building / Room)Date of Inspection: Inspected by:Follow-up Required: Yes NoEntrance to the containment zone(s) indicate room number(s)I.Containment Zone Entry#Lab facility1Door signage includes biohazard symbol, containment level, PIand emergency contact details, “authorized persons only”, andentry/exit requirements. Out-of-date signs are removed2Lockable entrance door3Self-closing doors4Delineated “clean” area at the entrance (e.g., separate vestibule,floor tape, color-coded tile)5Dedicated hand-washing sink (adjacent to entrance door);equipped with soap and paper towels. Hands-free78Storage space for PPE i.e. protective clothing, eyewear,respiratory equipment. “Clean” area separated from “dirty”Dedicated PPE used (not shared)9PPE available for visitors; cleaned/decon between uses10 Personal items incl. food and drinks stored outside thecontainment zone (indicate where)YESNONA

II. Physical Facilities1Lab clean, free of obstructions and free of excess materials2Walls non-porous (e.g., cement block) painted with waterresistant paint which can be decontaminated.3Floors seamless, slip-resistant and in good condition4Airflow - Inward without re-circulation(Smoke detector test done)5Closed and sealed windows6No porous surfaces on casework, doors, frames (gaps inbaseboards, no corkboards; surfaces can be easily cleaned anddecontaminated.7Non-absorptive benchtops; resistant to heat and chemicals.Painted surfaces not chipped or cracked.8Caulking on benchtops (sinks, walls) in good condition9Laboratory stools & chairs are non-fabric and /or covered withnon-porous material i.e. vinyl10 ANSI-approved Eyewash and shower, free of obstructions andtested weekly, the shower biannually minimum (checktags/logs).11 Sinks labelled with EHS warning sign regarding hazardous waste12 Dedicated paperwork / computer stations (located away fromwork areas. Station is in a separate room13 Plants and/or animals not related to work are not seen

III. Equipment: Biosafety Cabinet1Located away from doors, foot traffic, and supply air ducts2Certified within the past year; expiry date posted on the cabinet.3Clean and clutter free; No storage on top of the cabinet and/orinside; Air grilles not obstructed.4Alarm in working order5Location of external natural gas shut-off valve easily-accessibleIV. Equipment: CentrifugeLocation:1Rotors logged for usage2Safety cups available and in good condition (check O-rings)3Integrity of seals checked regularly (check log book)V. Equipment: Vacuum System1Adequate safeguards available to contain aerosols.Vacuum lines equipped with HEPA filters, traps, and flasks tapedand kept in secondary container to prevent tip offComments (if any):

VI. Equipment: AutoclaveLocation:1Current boiler inspection certificate with responsible personcontact information posted (Centralized )2Cycle record tapes are readily available (past two years)3Validation records are readily available and up-to-date4User logbook is readily available and up-to-date5Thermal-protection gloves are available for handling hot objectsVII. Equipment: Refrigerators, freezers & Dewar’sLocation:1Inventory stored inside the containment zone. If not, indicate where:2Storage equipment locked and access controlled at all times.3Storage equipment labelled with the biohazard symbol4Equipment with an alarm has signage indicating the emergencycontact information.5Refrigerators and freezers that are not designed for flammableliquid storage are labelled “flammable solvents prohibited”,Comments (if any):

VIII. Documentation1Current permit and associated documents readily available to allinvestigative staff2Training of all investigative staff is completed, and records areavailable on file3An “Agreement on Biosafety” form is on file for each member ofinvestigative staff4Current Pathogen Safety Data Sheets are readily available to allinvestigative staff5Current updated workplace-specific Laboratory SafetyManual/SOPS are readily available to investigative staff. (reviewedannually)6The Canadian Biosafety Standards are readily available.7The University of Guelph Biosafety Manual is readily available.8Standard operating procedures including working with the agent,waste disposal, and emergency management are readily availableto investigative staff9Equipment maintenance records are available10 Material transfer agreements and import permits are available11 Incident reports and investigation are available (if applicable)12 Workplace inspection records are available13 Data protected by encryption14 Inventory records are submitted to BSO (regularly updated, andsecure)15 Biological Spill plan template submitted to BSO and a copydisplayed in lab16 Annual Emergency Exposure Response Plan submitted to BSO

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IX. Waste Management1CSA-approved sharps waste containers readily available and notover-filled2Syringe needles not re-capped before disposal. (re-sheathing needlesare preferred)3Waste containers for larger broken glass items / sharp materials arereadily available, labelled, and leak-proof4Gloves discarded in the biohazardous waste container5Leak-proof containers (i.e. will not tip and spill the contents) readilyavailable6Plastic bags labelled with the biohazard symbol are readily availableand used only for biohazardous wastes.7Waste management protocols readily available8Approved disinfectant is readily available.X. Transport of Biohazardous Materials1Spill-proof cart available for moving materials (e.g., waste to theautoclave room)2Leak-proof impact resistant, clip on secondary containers (easilydisinfected) available (e.g., plastic totes)3When shipping off-campus, appropriate TDG-compliant packaging isavailableComments (if any):

XI. Emergency Management1Spill kit, based on a local risk assessment, is labelled, readilyavailable, and stocked.2First aid kit stocked and readily available. Dressings are waterproof.3Emergency telephone numbers posted (e.g., Campus CommunityPolice); a telephone is readily accessible.4Incident report forms are readily available5The fire alarm and fire extinguisher are not obstructed and easilyaccessible6Emergency egress routes posted7Refresher training on emergency procedures conducted areregularly. Date of most recent refresher training:XII. Personal Protective Equipment1“Howie” coats used for lab coats. (coats with knitted cuffs)2Laundry service provider used for the lab coats:3Flame-retardant protective clothing available when working withflammable liquids or pyrophoric materials.4Nitrile gloves are readily available in a variety of sizes5If working with cryogenic gases (liquid nitrogen), appropriate glovesand a face shield are readily available6Safety glasses worn in the work area. If there is a risk of splashes,then safety goggles are available. Glasses / goggles not shared.7Closed-toe non-cloth and heeled shoes worn. (Note. Dedicated workarea footwear must be separate from street shoes).

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7 Refresher training on emergency procedures conducted are regularly. Date of most recent refresher training: _ XII. Personal Protective Equipment 1 “Howie” coats used for lab coats. (coats with knitted cuffs) 2 Laundry service provider used for the lab coats: _ 3 Flame-reta