Nano, Capillary And Micro LC Columns - Cromatec

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BioLC Columns and AccessoriesNano, Capillary and MicroLC ColumnsDelivering high performance separationsto -omics researchOur columns have been specifically designed to combine high performance withease of use. From the “plug and spray “ EASY-Spray columns to the innovativenanoViper fitted Acclaim PepMap range and the Monolithic PepSwift andProSwift columns.t - .4 PNQBUJCMF 'PSNBUTOffering the highest sensitivityt CBS PNQBUJCMF Nano ColumnsThermo Scientific Chromatography Columns and Consumables 2016-2017With increased length to increase peak capacity3-038t .POPMJUIJD 1PMZNFS 1IBTFT For high throughput peptide and protein analysisThe Bio LC web page contains the latest news, applicationsand downloads for the nano, capillary and microbore range.Visit www.thermoscientific.com/columns

BioLC Columns and Accessories NanoLCTechnical ResourcesTechnical Resourcesvisit www.thermoscientific.com/chromexpertto access the following information:t Application Areas by Column Formatt tography3-039

BioLC Columns and AccessoriesEASY-Spray Columns“Plug and Spray” nano LC columns for routine and robust performance.t Simple to use formatt Routine research methodologyt Excellent performance for peptide separationst Available with wide pore phases and monoliths for the separation of intact proteinsAcclaim PepMap C18 100ÅParticle size (µm)23Length (mm)15025050075015050µm IDES801––––75µm IDES804ES802ES803ES805ES80050µm ID–75µm IDES812Acclaim PepMap C18 300ÅParticle size (µm)5Length (mm)150PepSwiftLength (mm)250200µm IDES810EASY-Spray EmittersIntegrated sprayer coupled with a transfer line.Thermo Scientific Chromatography Columns and Consumables 2016-2017t Pre-made connections3-040t Easy installation in the MS Ion Sourcet No chromatography requiredEASY-Spray EmittersDescriptionEASY-Spray Emitter, nanoflow (Emitter ID 7µm, Transfer line ID20µm, Transfer line length 500mm)EASY-Spray Emitter, microflow (Emitter ID 20µm, Transfer line ID75µm, Transfer line length 500mm)Cat. No.ES791ES792

Designed to be a virtually dead volume-free fingertight UHPLC fitting system.t Provides virtually zero-dead-volume connectionst Compatible with backpressures up to 1000 bar (14,500 psi)t Suitable for temperatures up to 80 Ct Easy to use 1/32" PEEK sheathed fused silica and fingertight designt Works with virtually any valve and any column from any manufacturerThe nanoViper connections come preassembled and high pressure tested. The fittings do not “grab” the tubing at a single point.It seals at the tip and not with a ferrule. Nor does it “grab” the tubing at a single point. It is this feature that gives nanoViper fittingstheir UHPLC, virtually dead-volume-free capabilities.nanoViper Fingertight FittingsLength (mm)Orange20µm 1.52806041.5122Black75µm �m 1.55806041.5125Red100µm 1.58156041.58246041.5816-Purple150µm 1.58226041.5823-*180mm LengthnanoViper Fingertight m ID6041.5118*-BioLC Columns and Accessories nanoViper Fingertight FittingsTrap Column Cartridges Holders with nanoViper FittingsDescriptionµ-Precolumn holder for trap cartridges with 2 x 100mmlength nanoViper fittingsCartridge Length (mm)515Fitting ID (µm)3075Cat. No.164649164650Sample Loops with nanoViper FittingsVolume (µL)15102050125Cat. 26.2412Quantity1 Each1 Each1 Each1 Each1 Each1 EachFind out more about how SMART Digestgest Kitscan benefit your workflow hermoscientific.com/chromatography3-041

BioLC Columns and AccessoriesThermo Scientific Chromatography Columns and Consumables 2016-20173-042nanoViper Application KitsnanoViper fittings are also included in tubing and application kits designedfor use with the UltiMate 3000 RSLCnano systems.nanoViper Fitting Kits for UltiMate 3000 RSLCnano SystemsDescriptionTubingSamplesTrap ColumnSeparation ColumnDirect Injection nano LC KitDirect Injection capillary LC KitPreconcentration nano LC kitPreconcentration capillary LC kitPreconcentration monolithic LC kit2D salt plugs kitAutomated off line SCX-RP peptides kitAutomated off line RP-RP peptides kitTandem nano LC kitFLM nanoViper tubing kit(All tubing for nano LC preconcentration)MS connection kitEASY-Spray connection kit(Supports direct injection as wellas .0395

The standard for peptide separations in proteomicst High-resolution protein identificationt Highest sensitivity in LC-MSt Fitted with nanoViper fittings as standardAcclaim PepMap C18 100ÅnanoViper FS75µm –Acclaim PepMap C8 100ÅParticle size (µm)3Length (mm)15050µm ID–nanoViper Column75µm ID300µm ID1647061647221000µm ID16472350µm ID–nanoViper Column75µm ID300µm ID1647071647201000µm ID16472150µm ID–nanoViper Column75µm ID300µm ID1647081647181000µm ID164719NanoLC ColumnsnanoViper ColumnLength (mm)50µm ID75µm ID300µm ID1000µm Note: nanoViper FS Columns have 45mm of Fused Silica for use with PEEK sleeves and Microtight unionsParticle size (µm)2BioLC Columns and Accessories Acclaim PepMap NanoLC columnsAcclaim PepMap C4 300ÅParticle size (µm)5Length (mm)150Acclaim PepMap C18 300 ÅParticle size (µm)5Length 3

BioLC Columns and AccessoriesAccucore NanoLC ColumnsAccucore 150-C18nanoViper ColumnParticle size (µm)2.6Length (mm)15050075µm ID16126-15756916126-507569Length (mm)150500nanoViper Column75µm ID16526-15756916526-507569Accucore 150-C4Particle size (µm)2.6Accucore 150-Amide-HILICParticle size (µm)2.6Length (mm)150nanoViper Column75µm ID16726-157569MSPac DS-10 MS Desalter CartridgeBuilt for LC-MS compatibilityt High confidence with low peak carryovert Antibody and protein desalting with a high load capacityt High pH stability: 0 - 14t IEX, pH-gradient, SEC fraction useThermo Scientific Chromatography Columns and Consumables 2016-2017t MS sensitivity improvement3-044MSPac DS-10 MS Desalter CartridgeItemDesalter Cartridge - 2 PackCartridge HolderCat. No.089170069580

High-speed peptide and protein separationst High-resolution for protein identification, biomarker discovery,and systems biologyt High column-to-column reproducibilityt nanoViper fittings for easy column installationThe monolithic structure offers a high-quality alternative to traditional microparticulatesorbents, providing important advantages to the chromatographic separation.High-sensitivity proteomics and biotech applications are easily performed usingthese columns.PepSwift100µm ID–164584164543500µm ID–164585–100µm ID–164922164921ProSwift200µm ID–164923–500µm ID164925––100µm ID–164929164928ProSwift200µm ID–164930–500µm ID164931164932–ProSwift RP-4HLength (mm)100250500ProSwift C4 RP-5HLength hyMonolithic Capillary and Micro HPLC ColumnsLength (mm)550250PepSwift200µm ID164558164557164542BioLC Columns and Accessories Monolithic Capillary and Micro HPLC Columns3-045

BioLC Columns and AccessoriesTrap columns – nano trap designAcclaim PepMap C18 100ÅTypenanoViper ColumnClassic ColumnPart number164535164705164564164197164199164213Particle Size335555Trap Columns – Cartridge (Set of 5)Acclaim PepMap C18 100Å300µm ID160454–1000µm ID160434160438Acclaim PepMap C8 100ÅLength (mm)5300µm ID161194Acclaim PepMap C4 300ÅLength (mm)5300µm ID163591Acclaim PepMap C18 300ÅLength (mm)5Thermo Scientific Chromatography Columns and Consumables 2016-2017Bed Length (mm)202020102020Note: 164705 is a shorter total length used for vented column set up for example with EASY nLC 1000Length (mm)5153-046ID7575100100100200300µm ID163589Accucore 150-C18Length (mm)5300µm ID16126-900379Accucore 150-C4Length (mm)5300µm ID16526-900379Accucore 150-Amide-HILICLength (mm)5300µm ID16726-900379μ-Precolumn holder, 5mm, with 30μm IDconnecting tubing, nanoViper fittingsLength (mm)5300µm ID164649Total Length15070150150150150Quantity2 Pack2 Pack2 Pack2 Pack2 Pack2 Pack

Improving separation efficiency with multidimensional analysis.There are dedicated application kits which utilize either SCX or RP as the first dimensionA TiO2 trapping column can be added to the application for phosphopeptide analysis1st dimension columnsPart Number164565ChemistrySCXDescription300μm ID x 10cm, packed with Poros 10 S,nanoViper column1.0mm ID x 15cm, packed with Polysulfoethyl ASP,5μm, 300Å, nanoViper column300μm ID x 15cm, packed with Acclaim PA2,nanoViper column100µm ID x 1cm packed with TiO 2200µm ID x 1cm packed with TiO 2164566RP164592TiO 2164205164215Application2D Salt plugsAutomated off-line 2D LC of Peptides,micro SCX x nano RPAutomated off-line 2D LC, Cap RP (basic) xNan RP (acidic)Phosphopeptide analysisPhosphopeptide analysisIsolation of two synthetic phosphopeptides from a BSA tryptic digestBreakthroughA3210x10 84Bound fractionB3SVENLPEAGlpTHEQR1758.8 Da2ENIMRpSENSESQLTSK1931.8 Da10510152025Minutes30354045Trap Column: 200μm ID x 1cm, packed with TiO2 , 5μm100μm ID x 1cm, packed with Acclaim PepMap C18, 5μmSeparation Column: Acclaim PepMap C18, 3μm, 75μm x 15cmLoading Solvent: 0.05% HFBA in DI H 2OWash Solvent:0.01% HFBA in DI H 2OMobile Phase A: 0.05% TFA in DI H 2OMobile Phase B:0.04% TFA in acetonitrile/DI H 2O (80:20 v/v)TiO 2 TrapMobile Phase:250mM NH 4HCO 3 in DI H 2O, pH 9.0Gradient:3–40% acetronitrile in 30 minFlow Rate:300nL/minLoading Flow:8μL/minInjection Volume: 5μLDetection:MSTrap Columnsx10 84BioLC Columns and Accessories 1st dimension columns for 2D separationsEASY-ColumnsExcellence in nanoscale separationst Compatible with any nanoscale HPLC systemt Simple, flexible designEASY-Column capillary LC columns are produced with a focus on design simplicity usingmetal-free fused capillaries and can be used on any nano LC systemEASY-ColumnsDescriptionEASY-Column, 2cm, ID 100µm, 5µm, C18-A1 (Trap column)EASY-Column, 10cm, ID 75µm, 3µm, C18-A2 (Analytical column)EASY-Column, 10cm, ID 75µm, 3µm, C18-A2 (Analytical column)HPLC-to-Column Connector kit Zero-dead-volume union(1/32in OD tubing), 10x SC603A/B Mixing Tee & Venting Tee for two-column setup Nanoliter-dead-volume tee(1/32in OD tubing), 10 sleeves for 360µm OD fused silica (10xSC603).Connector Kit for two-column setup Zero-dead-volume union(1/32in OD tubing), Nanoliter-dead-volume tee (1/32 inch OD tubing), 10x SC603Sleeves (2cm, 1/32in OD) for 360µm OD fused silicaCat. No.SC001SC200SC2003SC600Quantity3 Pack1 Each3 Pack1 EachSC6011 EachSC6021 EachSC60330 Packwww.thermoscientific.com/chromatography3-047

BioLC Columns and AccessoriesNano InstrumentsThermo Scientific nanoLC systems offer market leading performanceand integrate seamlessly with all Thermo Scientific nanoESI sourcesand MS familiesEASY-nLC1200Operational simplicity and excellent performancet Increased robustnesst Improved performance at 1200 bart Improved throughputt Intelligent maintenanceEASY-nLC1200Operational simplicity and excellent performancet Easily configured for routine nano LCt Wide range of flows available from 20nL/min to 50μL/minThermo Scientific Chromatography Columns and Consumables 2016-2017t Customized application set up easily established3-048For more product information visit our capillary-systems.html

750 – ES805 3 150 – ES800 Acclaim PepMap C18 300Å Particle size (µm) Length (mm) 50µm ID 75µm ID 5 150 – ES812 PepSwift Length (mm) 200µm ID 250 ES810 EASY-Spray Emitters Description Cat. No. EAS

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