MSc BIOTECHNOLOGY CHOICE BASED CREDIT SYSTEM (CBCS .
MSc BIOTECHNOLOGY CHOICE BASED CREDIT SYSTEM (CBCS)DEPARTMENT OF GENETICS & BIOTECHNOLOGY, OSMANIA UNIVERSITYSchedule for Instruction and Examination(Proposed Scheme for Academic year 2019 onwards)SyllabusS.No Ref NoSubjectSEMESTER- ITeachingHours4.THEORYBT 101 T Cell Biology and GeneticsBT 102 T Biological chemistryBT 103 T MicrobiologyStatistics, laboratory managementBT 104 T & safety,entrepreneurship1.2.3.4.PRACTICALSBT 151 P Cell Biology and GeneticsBT 152 P Biological chemistryBT 153 P MicrobiologyBT 154 P Biostatistics1.2.3.TotalSyllabusS. No Ref No1.2.3.4.1.2.3.4.SubjectBT 302 T Bioinformatics and itsApplicationsElective:BT 303 T Advances in Plant Biotechnology(or)Food BiotechnologyElective:BT 304 T Animal Biotechnology (or)Protein EngineeringPRACTICALSBT 351 P Recombinant DNA technologyBT 352 P Bioinformatics and itsApplicationsBT 353 P Advances in Plant Biotechnology(or)Food BiotechnologyBT 354 P Animal Biotechnology (or)Protein 50600SEMESTER- IIITeachingHoursTHEORYBT 301 T Recombinant DNA 01002244505050502450502450502432600
CBCS syllabus (2019 onwards)MSC BIOTECHNOLOGY-I YEARSEMESTER-ITHEORY PAPER-IBT 101 T- CELL BIOLOGY AND GENETICS1. Course Objectives (C. Obj.)a. Understand basic aspects of intracellular organization of a eukaryotic cellb. To comprehend the Mendelian Genetic principles in humans, plants andanimalsc. Obtain basic knowledge of hierarchical structure and organization ofchromosomes, insight into chromosomal anomalies and learn the sciencebehind gene mapping in eukaryotesd. To comprehend the biochemical and molecular processes of cell division andcell death2. Course Outcomes (C.O)a. Comprehend the cellular architecture and processesb. Ability to apply Mendelian ineritance principles to humans, plants and animalsc. Appreciate the importance of fidelity of chromosome organization and gain anability to localize genes by appropriate techniques.d. Knowledge regarding the basic mechanisms underlying cell division and celldeathCourse Plan / ScheduleUnitNo.Unit-11.1Topics to be coveredInternal Organization of CellMembrane structure– lipid bilayer, properties of lipid bilayer, lipidrafts, membrane proteins– peripheral and integral proteins, electricproperties of membrane1.2Structure and function of Endoplasmic reticulum, Structure andfunction of Golgi Complex, Structure and function of Lysosomes1.3Structure, biogenesis and functions of mitochondria1.4Structure, biogenesis and functions of chloroplast1.5Structure & function of cytoskeleton- introduction to microfilaments,intermediate filaments, microtubules, myosin structure and role inmotility1.6Intracellular protein transport- secretory pathway; protein transportinto nucleus, chloroplast and mitochondria; endocytosis andexocytosisUnit -2 Principles of Inheritance2.1Chromosome theory of inheritance; Mendel’s Laws - Law ofsegregation & Law of independent assortment- test cross and backcross2.2Extension to Mendel's Laws- Incomplete dominance (e.g. FlowerColor), Codominance (e.g. MN Blood groups); Non allelicinteractions- Types of Epistasis, modification of dihybrid ratios;No. oflectures232234231
CBCS syllabus (2019 onwards)2.32.42.52.6Unit 33.13.23.33.43.53.6Unit-4Multiple Allelism (e.g. Coat color in Rabbits, eye color in Drosophila,ABO Blood groups, Rh blood groups, S locus in Nicotiana)incompatibility and pseudoallelism, Complex loci- R-locus in maizePenetrance and Expressivity (e.g. Polydactyly, WaardenburgSyndrome), Pleiotropism (e.g. Bardet Biedel Syndrome, Marfansyndrome), Phenocopy (e.g. Microcephaly)Sex determination in Drosophila, Birds, Man and Bonellia; X-linkedinheritanceHemophilia, Color blindness, Lyonization; Y-linkedinheritance- Holandric genes; Sex limited and sex influencedcharacters; Inheritance patterns in Man-Pedigree analysisPolygenic inheritance, Additive effect- Skin color in man, Kernelcolor in maizeNon- Mendelian inheritance-Maternal inheritance-Variegation inleaves of higher plants- Mirabilis Jalapa, Poky in Neurospora,Maternal effect - Shell coiling in snails, Uniparental inheritancemutations in extra nuclear genes in Chlamydomonas, Male sterility inMaizeChromosomes– Organization and Aberrations, Linkage mappingChromosome- morphology, classification, Karyotyping; Features ofcentromere and telomere; Specialized chromosomes- Polytene & lampbrush chromosomes; Variation in chromosome number- Euploidy,Aneuploidy; Variation in chromosome structure -deletions,duplication, translocations and inversionsChromatin organization- Nucleosome, loops and scaffolds;Nucleosome phasing ; Chromatin under transcription- euchromatinand heterochromatinCytological proof of crossing over- Creighton and McClintock’sexperiment, correlation between chiasmata and crossing overPhases of linkage, test cross, recombination frequency, gene mapping,determination of map distances based on two and three point testcrosses, coincidence, interference (e.g. Drosophila, Maize)Tetrad analysis- Neurospora; Mitotic crossing over- Aspergillus,DrosophilaGene mapping and applicationsCell Division and Cell DeathCell cycle: Phases (interphase and M phase) and Check points in cellcycle, overview of mitosis and meiosisBiochemistry of cell division cycle: cyclins and CDKs, APC and SCFcomplexes, spindle machinery and molecular motorsMitosis: Stages and molecular mechanismsMeiosis: Stages and molecular mechanismsCellular processes: cell growth, cell differentiation, senescence,autophagyCell death: Necrosis, apoptosis (intrinsic and extrinsic pathways)23244322233423222
CBCS syllabus (2019 onwards)PRACTICALSBT 151 P: CELL BIOLOGY AND GENETICSS.No.1.2.3.4.5.6.Topics to be coveredPreparation of mitotic chromosomesPreparation of meiotic chromosomesStudy of polyploidy in onion root tipsKaryotyping of normal & abnormal chromosome setsPreparation of polytene chromosomesMonohybrid and dihybrid ratios ,Multiple alleles, Epistasis- Problems7.8.Inheritance patterns in man– pedigree analysisLocalization of genes– two & three point test crosses, Tetrad analysis– ProblemsREFERENCE BOOKS1. An introduction to Genetic Analysis by Anthony, J.F. J.A. Miller, D.T. Suzuki, R.C.Richard Lewontin, W.M-Gilbert, W.H. Freeman publication2. Principles of Genetics by E.J.Gardner and D.P. Snusted. John Wiley & Sons, NewYork3. The Science of Genetics, by A.G. Atherly J.R. Girton, J.F. Mcdonald, SaundernCollege publication4. Principles of Genetics by R.H. Tamarin, International edtn McGrawhill5. Theory & problems in Genetics by Stansfield, Schaum out line series McGrahill6. Cell & Molecular Biology. E.D.D De Robertis & E.M.F De Robertis, Waverlypublication.7. Molecular Biology of the cell. Alberts, B; Bray, D, Lews, J., Raff, M., Roberts, K andWatson, J.D. Garland publishers, Oxford8. Molecular Cell Biology Lodish, H., Baltimore, D; Fesk, A., Zipursky S.L.,Matsudaride, P. and Darnel American Scientific Books. W.H. Freeman, NewYork9. Cell and molecular biology by Gerald Karp, Wiley10. The cell: a molecular approach by Goeffrey Cooper and Robert Hausmann3
CBCS syllabus (2019 onwards)MSC BIOTECHNOLOGY-I YEARSEMESTER-ITHEORY PAPER-IIBT 102 T- BIOLOGICAL CHEMISTRY1. Course Objectives (C. Obj.):a.b.c.d.To learn the basics of chemistry related to bio moleculesTo understand the carbohydrate classification and metabolismTo comprehend metabolism of lipids, nucleic acids and amino acidsTo learn cellular signaling processes2. Course Outcomes (C.O)a.b.c.d.Understanding the basics about bio-molecules, bio-energetics and enzymologyAcquaintance with carbohydrate metabolism and networksCritically understanding biosynthesis of lipids nucleic acids and amino acidsUnderstanding of how cells communicate and carryout physiologicalprocessesCourse Plan/ ScheduleUnitNo.Topics to be coveredBiomolecules, Bioenergetics and EnzymologyImportance of water in biological systems, pH, Henderson- hasselbalchequationLaws of thermodynamics, Gibbs free energy, Enthalpy, Entropy,1.2CatalysisCarbohydrates (Classification-monosaccharides, disaccharides,1.3oligosaccharides & polysaccharidesLipids (Classification-fatty acids, Nucleic acids, Amino acids, Proteins1.4(Primary, secondary & tertiary structures)Proteins (Primary, secondary & tertiary structures)1.5Enzymes and enzyme kinetics, Briggs-Haldane reaction, Michaelis1.6Menten equation Coenzymes, Cofactors, enzyme regulationUnit -2 Carbohydrate metabolism and networksGlycolysis, TCA cycle and Electron transport chain2.1Gluconeogenesis, Glycogenesis and Glycogenolysis, Glucuronic acid2.2cyclePentose phosphate pathway2.3Entner-Doudoroff pathway, Cori cycle2.4Photosynthesis, C3 & C4 cycle2.52.6Overview of Carbohydrates networksUnit 3 Biosynthesis and metabolism of lipids, Nucleic acids and amino acidsHydrolysis of triacylglycerols3.1Unit-11.1No. oflectures23333233233224
CBCS syllabus (2019 oxidation, Fatty acid biosynthesis, Cholesterol metabolismBiosynthesis of amino acids, Amino acid degradation, Urea cycleProstaglandin biosynthesisNitrogen metabolism: Nitrate and ammonium assimilationBiosynthesis and degradation of purines and pyrimidinesCell communication and Singnalling pathwaysCell communication (autocrine and paracrine), Cell surfacereceptors in signal transductionSecond messengers and their role in signal transduction– cAMP, cGMP,phosphatidylinositol derived second messengers, calcium as second messengerG-protein coupled receptor– structure and function; GPCR signallingpathways; Ion channel receptorsTyrosine kinase linked receptors (receptors for cytokines), JAK-STATpathwayReceptors with intrinsic enzyme activity (RTK) and RTK signalingpathwaysWnt signalling pathway; Toll-like receptor signalling pathway33323233332PRACTICALSBT 152 P: BIOLOGICAL CHEMISTRYS. No.Topics to be covered1.Qualitative tests of sugars, amino acids and lipids2.Estimation of total sugars by DNS method3.Estimation of proteins by Lowry’s method4.Separation of amino acids by paper chromatography, thin layer chromatography(TLC)5.Separation of proteins by SDS-PAGE6.Enzyme assay- Catalase or Invertase7.Estimation of cholesterol by Zak and Henly’s method8.Amylase activity assay9.Plate assay for enzymes10.Column chromatography- Gel filtration (size exclusion)REFERENCE BOOKS1. Lehninger Principles of Biochemistry By: David L. Nelson and Cox2. Biochemistry:-By: Rex Montgomery.3. Harper’s Biochemistry. By: Robert K. Murray.4. Enzymes. By: Trevor Palmer.5. Enzyme structure and mechanism By: AlanFersht6. Principles of Biochemistry. By: Donald J. Voet, Judith G.Voet, Charlotte W.Pratt7. Analytical Biochemistry By Cooper8. Principles and techniques of Biochemistry and Molecular Biology Edited by KeithWilson and John Walker9. Experimental Biochemistry: A Student Companion by Sashidhar Beedu et al.10. Practical Biochemistry By Plummer5
CBCS syllabus (2019 onwards)MSC BIOTECHNOLOGY-I YEARSEMESTER-ITHEORY PAPER -IIIBT 103 T- MICROBIOLOGY1. Course Objectives (C.Obj)a. To learn about the General characteristics of microorganisms, microscopy,sterilization and containmentb. To learn about general characteristics of Bacteria; bacterial isolation, growth,culturing and preservationc. To learn about Viruses and their general characteristicsd. To learn about Algae, fungi & protozoa and their general characteristics2. Course Outcomes (C.O)a. Understanding the basics of microbiology and microbial classificationb. To culture different bacteria and know how to preserve themc. Acquaintance with culturing of viruses and viral pathogenesisd. Critical understanding of general characteristics and classification of algae, fungiand protozoaCourse Plan/ScheduleUnit No.Topics to be coveredUnit-11.11.2General characteristics of microorganismsMicrobiology- historical perspectiveMicroscopy and Applications- Principles and working of brightfield, Fluorescent, and Electron microscopesClassification of microorganismsConcept and methods of sterilization and their application inindustry- dry heat, moist heat, radiation methods, filtrationmethods, chemical methodsConcept of containment facilityTypes of antimicrobial agents and development of resistance bymicroorganisms to various chemicalsBacteria and their characteristicsGeneral characteristics of bacteria Identification methods forbacteria- conventional (simple staining methods, differentialstaining, structural staining and special staining method),molecular based approachesGeneral methods for isolation of bacteria- plating methods(streak, spread and pour plate methods); serial dilution.Bacterial growth- typical growth curve- batch and continuouscultures, synchronous cultures; Measurement of bacterial growthmeasurement of cell number and cell mass; factors influencingbacterial growth- temperature, pH.Pure cultures- concept of pure culture, methods of pure culture,Enrichment culturing techniques, single cell isolation and pureculture development1.31.41.51.6Unit-22.12.22.32.4No. oflectures32233322336
CBCS syllabus (2019 4.34.44.54.6Methods of preservation of microbial cultures– repeatedsubculturing, preservation at low temperature, sterile soilpreservation, mineral oil preservation, deep freezing and liquidnitrogen preservation, freeze-drying (lyophilization).Diseases caused by bacteria in humans (Staphylococcus,Streptococcus, Mycobacterium tuberculosis)Viruses and their characteristicsGeneral characteristics of virusesClassification of viruses and important characters of each groupStructure and replication of Bacteriophage (T2), Lambda phageLytic and Lysogenic cyclesIsolation and Purification of viruses by Filtration, Precipitationand CentrifugationMethods of cultivation of viruses- in animal cell inoculation,Chick embryo, tissue culture; Bacteriophage cultivation; cellculture method.Structure and general characteristics of important viruses (TMV,HIV, Hepaptitis virus, Polio virus, Prions) and Importance ofviruses in biotechnologyAlgae, fungi & protozoa and their characteristicsGeneral characteristics, vegetative & reproductive structure ofAlgae (Cyanophyta, Cholorophyta, Phacophyta, Rhodophyta)Economic importance of algaeGeneral characteristics of Fungi (Phycomycetes, Bacidiomycetes,Ascomycetes, Deuteromycetes)Fungi as pathogens of humans, plants and animalsGeneral characteristics of ProtozoaProtozoan as pathogens of humans ( Entamoeba, Plasmodium)332333233333317
CBCS syllabus (2019 onwards)PRACTICALSBT 153 P: MICROBIOLOGYS.No.1234Topics to be coveredMicrobiology- historical perspectiveMicroscopy and Applications- Principles and working of bright field,Fluorescent, and Electron microscopesClassification of microorganismsConcept and methods of sterilization and their application in industry- dry heat,moist heat, radiation methods, filtration methods, chemical methods5Isolation and Culturing of bacteria, fungi and algae: Tube culture (slant/broth), plateculture, flask cultureMeasurement of microbial growth (Viable count and turbidometry) Study of bacterialgrowth curve67Concept of containment facility, sterilization at industrial levelTypes of antimicrobial agents and development ofmicroorganisms to various chemicalsresistancebyREFERENCE BOOKS1. Microbiology by M.J. Pelzar, E.S.N. Cfan and N.R. Kreig, McGraw Hill Publ.2. Introductory Microbiology by J. Heritage, E.G.V. Erans, R.A. Killington, CambridgeUniversity Press.3. General Microbiology by H.G.Schlegel Cambridge University Press.4. General Microbiology by Stanier, R.Y, J.L. Ingrahm, M.L. Wheel is & P.R. Painter.5. Microbiology– Concepts and Application. John Wiley and Sons, New York8
CBCS syllabus (2019 onwards)MSC BIOTECHNOLOGY-I YEARSEMESTER-ITHEORY PAPER - IVBT 104 T- BIOSTATISTICS, LABORATORY MANAGEMENT & SAFETY,ENTREPRENEURSHIP1. Course Objectives (C.Obj.)a. To understand the significance of sampling & data alignmentb. To understand the concept of applying appropriate test statisticsc. To learn Laboratory Management & Safetyd. To know the importance of Entrepreneurship2. Course Outcomes (C.O)a. Learn to estimate appropriate descriptive measures for a data in a given studydesignb. Help in derive inferences based on the statistical comparisonsc. Good laboratory practiced. How to be a successful entrepreneurCourse Plan/ScheduleUnit No. Topics to be .52.6Descriptive StatisticsRandom sample- Methods of sampling, study design, samplesize, effect of sampling biasTypes of variables (qualitative, quantitative and categorical)data alignment and representation-Frequency distributionHistogram, Frequency polygon, Pie Chart-Bar diagramMeasures of central tendency- mean, median, modePoint and interval estimates - Measures of Dispursion –coefficients – moment – skewness - curtosisConcepts of probability – types of events (dependent,independent and Mututally exclusive) – laws of probability(Addition & multiplication) – Bayesian theorem & itsapplicationsProbability distributions & applications – Normal, Binomialand PoissonInferential StatisticsConcept of Test of hypothesis, Null & Alternative hypothesis,level of significance, p-value, Class limits, Class intervalsLarge Sample Tests- Z-test of MeansSmall sample test - T-test for MeansChi square test & its applicationsAnalysis of Variance and Co-variance, One-Way ANOVA,Two-way ANOVASimple regression and correlation - Test of regressionNo. oflectures2323333223339
CBCS syllabus (2019 4.54.6coefficient and correlation CoefficientLaboratory Management & SafetyAdministration of Laboratories, Laboratory design, Securitymeasures, laboratory bio security concepts, LaboratoryInformation management system (LIMS)Laboratory safety- good laboratory practice (GLP), Biosafetylevels, Safety policiesBasic principles of quality control (QC) and quality assurance(QA)Handling of Hazardous compounds- chemicals, solvents,poisons, isotopes, explosives and biological strains (Bacterial,Fungal etc.)Storage of hazardous materialDisposal of biological and radioisotope wastesEntrepreneurshipConcept, definition, structure and theories of entrepreneurshipTypes of start-upsTypes of entrepreneurship, environment, process ofentrepreneurial developmentEntrepreneurial culture, entrepreneurial leadershipProduct planning and development -Project management,Search for business idea, Concept of projects, Projectidentification, Formulation, Design and network analysis,Project report and project appraisalPromoting bio-entrepreneurship.23332333333110
CBCS syllabus (2019 onwards)PRACTICALS:BT 154 P: BIOSTATISTICSS.No.1.Topics to be coveredEstimation of mean, median and mode for grouped and ungrouped data2.3.Variance, standard deviation and standard errorProblems on Probability distribution- Binomial, Normal, PoissondistributionsProblems on Chi square testLarge sample test (Z-test)Small sample test (T-test)Calculation of correlation and regression coefficientsProblems on ANOVA4.5.6.7.8.REFERENCE BOOKS1. Quantitative Genetics By Falconer2. Biostatistics By Vishweswara Rao3. Biostatistics By Khan and Khanum4. Fundamentals of Biostatistics By P.H. Rao and Janardhan5. Population Genetics By V. Venugopal and Pratibha Nallari6. Biostatistical Methods in Agriculture Biology and Medicine By Khan and Khanum7. Guides to entrepreneurship in biotechnology by P. Ponnumurugan, J Robinson and B.Kalpana8. Guidelines for entrepreneurship development program for biotechnology graduates byP. Ponnumurugan and Nithya. B9. CRC handbook of laboratory safety by A. Keith Furr11
4. Enzymes. By: Trevor Palmer. 5. Enzyme structure and mechanism By: AlanFersht 6. Principles of Biochemistry. By: Donald J. Voet, Judith G.Voet, Charlotte W.Pratt 7. Analytical Biochemistry By Cooper 8. Principles and techniques of Biochemistry and Molecular Biology Edited by Keith Wilson and John Walker 9.
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Unit B: Plant Biotechnology 75 1. Overview of Biotechnology (1 3 10 hrs) 14 hrs Introduction : A) Origin and History of biotechnology, B) Scope and importance of biotechnology: a) Biotechnology in Medicine, b) Biotechnology in food industry, c) . Dubey R.C. 2009. A text Book of Biotechnology
The MSc(Eng), MSc(CompSc) and MSc(ECom&IComp) curricula are offered in part -time and full- time modes. MSc 1 Admission requirements . To be eligible for admission to the curriculum leading to the degree of MSc(Eng) / MSc(CompSc) / MSc(ECom&IComp), a candidate shall: (a) comply with the General Regulations;
What is agricultural biotechnology? Biotechnology is a broad collection of tools and technolo-gies that involve the manipulation of living cells and/or biological molecules to solve problems and make useful products. Agricultural biotechnology is the application of biotechnology to agriculture. Agricultural biotechnology
UNIT I - INTRODUCTION TO BIOTECHNOLOGY . Lesson 1: An Overview of Biotechnology . Competency/Objective: Summarize the importance of biotechnology to agriculture. Study Questions . 1. What is biotechnology? 2. What has been the role of biotechnology in agr iculture? 3. What is the current
Biotechnology BT 20412 BT Plant Biotechnology 3 1 - 20 15 15 50 70 120 4 3 Biotechnology BT 20413 BT Food Biotechnology 3 1 - 20 15 15 50 70 120 4 4 Biotechnology BT 20414 BT Recombinant DNA Technology 3 1 . A textbook of Biotechnology by R.C. Dubey, S.Chand & company Ltd. References Books:
MSc Drilling and Well Engineering* 11 MSc Oil and Gas Engineering* 11 MSc Petroleum Production Engineering* 11 MSc Solar Energy Systems** 11 * Pre- masters in Engineering (Oil and Gas): ILS005 ILSC PG1006 Maths for Engineering and Sciences PG 1008 Introduction to Oil and Gas ** Pre-masters in Enginee
MSc Civil Engineering Structures (T) MSc Civil Engineering Structures (Nuclear Power Plants(T) MSc Analysis and Design of Structures for Hazards (T) MSc Analysis and Design of Structures for Fire, Blast and Earthquakes. MSc Professional Engineering (Civil Engineering) (T) MSc Construction Management 1 year 2 - 3 years 1 year 2 - 3 years 1 year
