INSTRUCTION MANUAL HITACHI FLUORESCENCE SPECTROPHOTOMETER .
INSTRUCTION MANUALHITACHI FLUORESCENCE SPECTROPHOTOMETERFL SOLUTIONS PROGRAM(OPERATION MANUAL)Hitachi High-Technologies CorporationCopyright Hitachi High-Technologies Corporation 2001.All rights reserved.Printed in Japan.3rd Edition, November 20011st Edition, 2001Part No. 251-9067-2 TF-TF (GT-LT)
PREFACEThank you very much for purchasing the HitachiHigh-Technologies Corporation fluorescence spectrophotometer.This instrument is intended for use by persons having abasic knowledge of chemical analysis. Remember thatimproper use of analytical instruments, chemicals orsamples would result not only in wrong analytical data butalso in consequences adverse to safety.Carefully read this instruction manual before attemptingoperation. For proper use of the fluorescencespectrophotometer, please acquaint yourself with it.ABOUT THIS MANUALThe Hitachi High-Technologies Corporation fluorescencespectrophotometer is accompanied by the following twoinstruction manuals: F-2500/F-4500 Fluorescence Spectrophotometer InstructionManual - Maintenance Hitachi High-Technologies Corporation FluorescenceSpectrophotometer Instruction Manual - FL Solutions Program- OperationThe cautionary instructions concerning safety are contained inthe F-2500/F-4500 Fluorescence Spectrophotometer InstructionManual - Maintenance. Be sure to read them before attemptingoperation.This instruction manual describes the operational procedures forthe FL Solutions program formulated for the Hitachi HighTechnologies Corporation fluorescence spectrophotometer.Be sure to read through this instruction manual before attemptingoperation.-1-
-2-
IMPORTANTPrecautions on Electromagnetic Wave Interference1.Possible Electromagnetic Wave Interference Caused byThe InstrumentWhen the instrument is used in a residential area or anadjacent area thereto, it may cause interference to radioand television reception.To prevent this, use the specified system connection cablesin strict accordance with the instruction manual.The instrument is designed to minimize possibleelectromagnetic wave interference caused by it if thespecified cables are connected properly.However, there is no guarantee that electromagnetic waveinterference will not be caused by the instrument.If the instrument does cause interference to radio ortelevision reception, which can be determined by turningthe instrument off and on, the user is encouraged to try tocorrect the interference by one or more of the followingmeasures: Increase separation between the instrument and theradio/TV receiver. Connect the instrument to an outlet on a circuit differentfrom that to which the radio/TV receiver is connected.2.Possible Electromagnetic Wave Interference Affectingthe InstrumentIf the instrument is used near an intense electromagneticsource, interference noise may be given to the instrumentto incur an adverse effect on its performance orfunctionality.To present this, use the specified system connection cablesin strict accordance with the instruction manual.The instrument is designed to minimize possibleelectromagnetic wave interference affecting it if thespecified cables are connected properly.However, there is no guarantee that electromagnetic waveinterference will not occur in the instrument. If theinstrument does incur electromagnetic wave interference,which can be determined by turning on and off possiblesources of electromagnetic interference nearby, the user isencouraged to try to correct the interference by one or moreof the following measures:-3-
Reorient the instrument. Increase separation between the instrument andpossible sources of electromagnetic interference. Increase separation between the power cable of theinstrument and possible sources of electromagneticinterference. Connect the instrument to an outlet on a circuit differentfrom that to which possible sources of electromagneticinterference are connected. Check that any other device connected with theinstrument is not affected by electromagneticinterference.-4-
WARRANTY ON PRODUCTThe Hitachi fluorescence spectrophotometer is warranted to befree from defects in material or workmanship under normal usewithin the product specifications indicated in this manual andunder conditions given below. This warranty is void if theinstrument is not used in accordance with the instruction manual.(1)Scope of Warranty(a) Any parts which prove to be defective in design orworkmanship during the warranty period will berepaired, adjusted or replaced without charge.(b) A substitute part may be used for repair, orreplacement with an equivalent product may be madeinstead of repair.(c) Such system components as a personal computer andprinter to be updated frequently for improvement maynot be available in original versions at the time ofreplacement.(2)Warranty PeriodOne year from the date of initial installation(Excluding consumables such as a xenon lamp)(3)Availability of Technical Support ServiceTechnical support service for the instrument is availablewithin regular working hours on workdays predetermined byus.(4)Limitations and Exclusions on WarrantyNote that the following cases are excluded from the scopeof this warranty.(a) Failure due to operation at a place not meeting theinstallation requirements specified by us.(b) Failure due to power supply voltage/frequency otherthan specified by us or due to abnormality in powersupply.(c) Corrosion or deterioration of the tubing due toimpurities contained in reagent, gas, air or coolingwater supplied by the user.(d) Corrosion of the electric circuits or deterioration of theoptical elements due to highly corrosive atmosphericgas.-5-
(e) Failure due to use of hardware, software or spare partsother than specified by us.(f) Failure due to improper handling or maintenance bythe user.(g) Failure due to maintenance or repair by a serviceagent not approved or authorized by us.(h) After disposal of the instrument, or after its resalewithout our approval.(i) Failure due to relocation or transport after initialinstallation.(j) Failure due to disassembly, modification or relocationnot approved by us.(k) Consumables, and failure of parts that have reachedthe end of specified useful life.(l) Failure of parts excluded from the warranty in theinstruction manual or other documents.(m) Failure due to acts of God, including fire, earthquake,storm, flood, lightning, social disturbance, riot, crime,insurrection, war (declared or undeclared), radioactivepollution, contamination with harmful substances, etc.(n) Failure of the hardware, or damage to the systemsoftware, application software, data or hard disk due tocomputer virus infection.(o) Failure of the personal computer connected with theinstrument, or damage to the system software,application software, data or hard disk due to powerinterruption or momentary power voltage drop causedby lightning or the like.(p) Failure of the personal computer connected with theinstrument, or damage to the system software,application software, data or hard disk due todisconnection of main power to the personal computerwithout taking the specified normal shutdownprocedure.-6-
DISCLAIMER OF WARRANTY(1)WE MAKE NO WARRANTIES, EITHER EXPRESS ORIMPLIED, EXCEPT AS PROVIDED HEREIN, INCLUDINGWITHOUT LIMITATION THEREOF, WARRANTIES AS TOMARKETABILITY, MERCHANTABILITY, FOR APARTICULAR PURPOSE OR USE, OR AGAINSTINFRINGEMENT OF ANY PATENT. IN NO EVENTSHALL WE BE LIABLE FOR ANY DIRECT, INCIDENTALOR CONSEQUENTIAL DAMAGES OF ANY NATURE, ORLOSSES OR EXPENSES RESULTING FROM ANYDEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.(2)NO ORAL OR WRITTEN INFORMATION OR ADVICEGIVEN BY US, OUR DEALERS, DISTRIBUTORS,AGENTS OR EMPLOYEES SHALL CREATE AWARRANTY OR IN ANY WAY INCREASE THE SCOPEOF THIS WARRANTY.Installation, Relocation and After-sale Technical ServiceInstallation of the instrument shall be carried out by or undersupervision of our qualified service personnel or our authorizedservice agent.Before installation of the instrument, the customer shall makepreparations for satisfying the installation requirements inaccordance with this manual.When relocation of the instrument becomes necessary afterinitial installation (delivery), please notify our local salesrepresentative or service office nearest you.Technical Seminars and Training for CustomersFor the customers to acquire in-depth understanding of theanalytical instruments, technical seminars and customer trainingcourses are available at our or your site. For further information,contact our local sales representative.(The technical seminars and customer training courses areavailable at charge.)-7-
OTHER PRECAUTIONSHandling of Chemicals and Samples(1)The user is responsible for following relevant legalstandards and regulations in the handling, storage anddiscarding of chemicals and samples used in analyticaloperations of the instrument.(2)Reagents, standard solutions and accuracy-controlsamples shall be handled, stored and discarded asinstructed by the respective suppliers.Notice on This Instruction Manual(1)The information contained in this manual is subject tochange without notice for product improvement.(2)This manual is copyrighted by Hitachi High-TechnologiesCorporation with all rights reserved.(3)No part of this manual may be reproduced or transmitted inany form or by any means without our express writtenpermission.Trademark AcknowledgmentsMicrosoft, Windows, Microsoft Excel, Microsoft Word, andWindows 98 are registered trademarks of Microsoft CorporationUSA.All other brand names and product names used in this manualare trademarks, registered trademarks, or trade names of theirrespective holders.-8-
SAFETY SUMMARYPRECAUTIONS ON SAFETYBefore using the Hitachi fluorescence spectrophotometer, besure to read the following safety instructions carefully.General Safety Guidelines Follow all the operating procedures provided in this manual. Installation and maintenance of this instrument shall becarried out by service personnel qualified therefore. Be sure to observe the warnings indicated on the product andin the instruction manual. Failure to do so could result inpersonal injury or damage to the product. The hazard warnings which appear on the warning labels onthe product or in the manual have one of the following alertheadings consisting of an alert symbol and signal wordDANGER, WARNING, or CAUTION.DANGER: Indicates an imminently hazardoussituation which, if not avoided, will resultin death or serious injury.(This warning does not apply to thisproduct.)WARNING : Indicates a potentially hazardoussituation which, if not avoided, can resultin death or serious injury.CAUTION : Indicates a hazardous situation which, ifnot avoided, will or can result in minor ormoderate injury, or serious damage tothe product.: The alert symbol shown at left precedesevery signal word for hazard warnings,and appears in safety-relateddescriptions in the manual."NOTE" and "NOTICE" are heading words which do not concernpersonal safety directly.SAFETY - 1
SAFETY SUMMARYGeneral Safety Guidelines (Continued)NOTICE :NOTE:Used to indicate an instruction for preventingdamage to the product.Used to indicate an instruction for ensuringcorrect use of the product and accurate analysistherewith. Do not modify the instrument, replace parts that are not userserviceable, use non-specified parts, nor remove safetydevices, as it could be hazardous. Installation at delivery, maintenance and relocation should bereferred to service personnel qualified by us. Do not perform any operation or action other than describedin this manual. When in doubt, please contact our localsales representative or service office nearest you. When using a chemical for analytical operation, be sure toprovide proper ventilation in the laboratory room as per localrequirements. Inadequate ventilation could endanger yourhealth. Keep in mind that the hazard warnings in this manual or onthe product cannot cover every possible case, as it isimpossible to predict and evaluate all circumstancesbeforehand.Be alert and use your common sense.SAFETY - 2
SAFETY SUMMARYSafety Notices on Fluorescence SpectrophotometerElectricity Make sure that the fluorescence spectrophotometer issupplied with power of 100V AC, 1 kVA or more (50 Hz or60 Hz). If the power voltage fluctuates or noise is applied tothe power line, the spectrophotometer may be adverselyaffected, resulting in any trouble in it. Be sure to provide grounding connection together with powerline connection. The grounding resistance should be 100ohms or less (conforming to Class-D grounding installation ofelectric facility technical standards). Check that thegrounding connection is proper. If the grounding connectionis improper, the spectrophotometer may become vulnerable toexternal noise or stray voltage may be produced in it, resultingin an electric shock hazard. The high voltage circuit is used inside the spectrophotometer.To prevent electric shock, do not open covers duringoperation unless otherwise necessary.Source of Heat/FlameDo not smoke or hold a flame near the fluorescencespectrophotometer.Data BackupData stored on the hard disk may become unusable due to asystem failure, wrong operation, computer virus infection, etc.To ensure data integrity in case of accidental damage to the harddisk, periodically make backup copies of hard disk files ontofloppy disks.To prevent an erroneous operation on the hard disk, alwaysreserve approx. 100 MB of free space as a working area forapplication software.SAFETY - 3
SAFETY SUMMARYProtection against Computer VirusesIf any program/data is damaged suddenly or an unexpectedoperation/screen is encountered, the personal computer issuspected of being infected by a computer virus.Computer viruses are malicious programs that sneak intopersonal computers to cause misbehavior or damage to data.And, a program designed to offer protection against computerviruses is called a vaccine program.Possible causes of virus infection are: Downloading a virus-laden program through communication. Using a floppy disk or other storage medium infected by avirus.Note also that once any personal computer is infected by a virus,it may spread to other computers via communication or storagemedium.Never use a program or storage medium that is suspectedof containing a virus.If there is a possibility of virus infection, check for a virus using avaccine program. Note, however, that some kinds of vaccineprograms cannot eradicate particular viruses. So, be sure tomake a backup of hard disk files beforehand.The user is requested to prepare a vaccine program and carryout virus removal on his or her own responsibility.Power InterruptionOn occurrence of momentary power voltage drop due to powerinterruption or lightning, the personal computer may becomefaulty or the system software, application software or data maybe damaged. For protection against momentary power voltagedrop, it is advisable to use an AC uninterruptible power supplyunit (stated according to the Japanese Electronic IndustryDevelopment Association guidelines for protection againstmomentary power voltage drop in personal computers).SAFETY - 4
SAFETY SUMMARYTurning On/Off Personal ComputerDo not turn off power to the personal computer while the harddisk or floppy disk drive is active. If power to the personalcomputer is turned off while the hard disk or floppy disk is beingaccessed, the personal computer may become faulty ordata/software stored in it may be damaged.Before turning off power to the personal computer, be sure toquit the fluorescence spectrophotometer control/data processingprogram (FL Solutions program) first and then take the shutdown procedure using the system software.SAFETY - 5
FOREWORD This instruction manual is intended for the users of the Hitachifluorescence spectrophotometer. It describes the installation,maintenance, operation and measurement procedures. The fluorescence spectrophotometer is designed for thepurpose of fluorescence analysis. Never use this instrumentfor any other purposes. The fluorescence spectrophotometer is intended for use bypersons having a basic knowledge of chemical analysis.Other persons should use them in the presence of a personfamiliar with chemical analysis. Remember that improperuse of analytical instruments, chemicals or samples wouldresult not only in wrong analytical data but also inconsequences adverse to safety. Before attempting installation/maintenance of thefluorescence spectrophotometer, read the instruction manualcarefully and fully understand the cautionary instructions forthe sake of safety. Keep this manual handy for easy reference when necessary. The information contained in this manual is subject to changewithout prior notice.FOREWORD 1
CONTENTSPREFACE .1ABOUT THIS MANUAL .1IMPORTANT .3Precautions on Electromagnetic Wave Interference .31. Possible Electromagnetic Wave Interference Causedby the Instrument .32. Possible Electromagnetic Wave Interference Affectingthe Instrument .3WARRANTY ON PRODUCT .5Installation, Relocation and After-sale Technical Service .7Technical Seminars and Training for Customers .7OTHER PRECAUTIONS .8Handling of Chemicals and Samples .8Notice on This Instruction Manual .8Trademark Acknowledgments .8SAFETY SUMMARY. SAFETY-1PRECAUTIONS ON SAFETY . SAFETY-1General Safety Guidelines . SAFETY-1Safety Notices on FluorescenceSpectrophotometer . SAFETY-3Electricity . SAFETY-3Source of Heat/Flame . SAFETY-3Data Backup . SAFETY-3Protection against Computer Viruses . SAFETY-4Power Interruption . SAFETY-4Turning On/Off Personal Computer . SAFETY-5FOREWORD .FOREWORD-1-i-
1.FUNCTIONS AND SCREEN COMPOSITION . 1-11.1Functions . 1-11.2Installing the FL Solutions Program . 1-51.3Startup of FL Solutions Program . 1-91.4Screen Composition . 1-131.4.1 Basics of Window Operation . 1-131.4.2 Kinds of Windows . 1-171.4.3 Data Processing Window . 1-181.4.4 Explanation of Tool Buttons . 1-211.5Functions of Function Keys . 1-261.6Explanation of Monitor Window . 1-271.7Input of Spectrophotometer Serial No. . 1-301.8Window Setting . 1-311.9Saving a Data File . 1-391.1
Manual - Maintenance Hitachi High-Technologies Corporation Fluorescence Spectrophotometer Instruction Manual - FL Solutions Program - Operation The cautionary instructions concerning safety are contained in the F-2500/F-4500 Fluorescence Spectrophotometer Instruction Manual - Maintenance. Be sure to read them before attempting operation.
Practical fluorescence microscopy 37 4.1 Bright-field versus fluorescence microscopy 37 4.2 Epi-illumination fluorescence microscopy 37 4.3 Basic equipment and supplies for epi-illumination fluorescence . microscopy. This manual provides basic information on fluorescence microscopy
82 hitachi zx200-3, zx225usrlc-3 1033091 sprocket sp-zx200-3 83 hitachi zx240-3 1032489 sprocket 84 hitachi ex300-1/2, ex270-1/2 1010467 sprocket 80 sp-ex300-1 85 hitachi ex300-3,ex270-5 1017928 sprocket 80 sp-ex300-3 86 hitachi ex300-5, ex330-5, zx270, zx330, zx350, zx370 1022168 sprocket 85 sp-ex300-5 87 hitachi
Specialized Fluorescence Techniques 171 Single Molecule Fluorescence 172 Fluorescence Correlation Spectroscopy 173 Forster Resonance Energy Transfer 173 Imaging and Super-Resolution Imaging (Con-ventional and Lifetime) 174 Instrumentation and Laser Based Fluorescence Techniques 175 Nonlinear Emission Processes in Fluorescence Spectroscopy 176
HITACHI MELBOURNE HITACHI MELBOURNE 50,000.00 GST 55,000.00 55,000.00 GST 60,500.00 Hitachi ZX48U-5 Excavator VIC, NSW, TAS, SA, ACT Jan 2019 Hitachi ZX48U-5 Excavator ID: D00030942 ROPS CAB, HBP, NEW HYD QH, 300MM, 45
Hitachi TagmaStore Network Storage Controller" omitted from document titles. For example, Hitachi TagmaStore Universal Storage Platform and Hitachi TagmaStore Network Storage Controller Storage Navigator User’s Guide is shortened to Storage Navigator User’s Guide. This user's
Status Report - BWRX-300 (GE Hitachi and Hitachi GE Nuclear Energy) USA DATE (2019/9/30) The BWR-300 is the 10th generation Boiling Water Reactor (BWR) crated by GE Hitachi Nuclear Energy (GEH). It is a SMR evolution of the ESBWR which is licensed by the US NRC and utilizes many of the components for the operational
P.R. Selvin (2000) The renaissance of fluorescence resonance energy transfer. Nat Struct Biol.7:730-4. P.R. Selvin (1995) Fluorescence resonance energy transfer. Meth Enzymol246:300-334. J.R. Lakowicz (2006) Principles of Fluorescence Spectroscopy, 3rd edn. Springer. Olympus Resource Center: Fluorescence resonance energy transfer
quality results of AGMA Class 10 with a double cut cycle and AGMA Class 9 with a single cut cycle can be expect-ed. The 100H weighs 7,100 lbs with a cube size of 78” X 64” X 72” high. For more information, contact Bourn & Koch at (815) 965-4013, Boeing F/A-18E/F Super Hornet Completes First AESA Flight In a press release dated August 13, 2003, it was announced that the Boeing F/A-18E/F .
