Fundamentals Of Enzymology
Fundamental sof Enzymolog yThe Cell and Molecular Biolog yof Catalytic Protein sTHIRD EDITIO NNicholas C. PriceLewis Stevens
A note on units xvList of abbreviations xvi iChapter 1Introduction11 .2Aims of this bookHistorical aspects1 .3Remarkable properties of enzymes as catalysts1 .4Cofactors1 .5Nomenclature and classification of enzymes1 .6The contents of this book1 .11147813References 1 4Chapter 2The purification of enzymes152 .1Introduction2 .2Why isolate enzymes?2 .3Objectives and strategy in enzyme purification2 .4Choice of source2 .5Methods of homogenization2 .6Methods of separation2 .72 .8How to judge the success of a purification procedureExamples of purification procedures 3 62 .9Conclusions from the examples of enzyme purification1515161720213443References 4 4Chapter 3The structure of enzymes473 .2Introduction 47The determination of Mr3 .3The determination of amino-acid composition and primary structure3 .14854
3 .4The determination of secondary and tertiary structure3 .53 .6The determination of quaternary structureThe unfolding and folding of enzymes 10 63 .7Concluding remarksReferences11 011 1Appendix 3 .111 6References for Appendix 3 .1Chapter 47510011 7An introduction to enzyme kinetics4 .1Outline of the chapter4 .2How do we obtain kinetic data?4 .3How do we analyse kinetic data?4.4Pre-steady-state kinetics4.5Concluding remarks11 811 811 912 114 4148References 14 8Appendix 4 .1 15 0Appendix 4 .215 1Appendix 4 .315 2References for AppendicesChapter 515 3The mechanism of enzyme action1545 .1Introduction5 .2Definition of the mechanism of an enzyme-catalysed reactionBackground to catalysis 15 55 .315 45 .4Experimental approaches to the determination of enzym emechanisms 1625 .5Examples of enzyme mechanisms5 .6Concluding comments on enzyme mechanismsReferencesChapter 617 721 2The control of enzyme activity21 76 .1Introduction6 .2Control of the activities of single enzymes6 .3Control of metabolic pathways6 .4Examples of control of metabolic pathways21 7Concluding remarksReferences 2666 .5Appendix 6.1Chapter 726 627 0Enzymes in organized systems7 .1Introduction7 .2Organized enzyme systems27 327321 823 727 324921 2154
coli7 .3RNA polymerase from7 .4The occurrence and isolation of multienzyme proteins7 .57 .6Phylogenetic distribution of multienzyme proteinsProperties of multienzyme proteins 28 37 .7Pyruvate dehydrogenase multienzyme complex and related systems7 .8Glycine decarboxylase multienzyme complex 29 4The tryptophan synthase multienzyme complex from7 .9E.27428 128 228 5E. coil 2947 .10Carbamoyl phosphate synthase and the associated enzymes of th epyrimidine and arginine biosynthetic pathways in E. coil, fungi, an dmammalian cells 30 07 .11Multienzyme polypeptides : fatty-acid synthase and the arom complex(AROM enzyme) 3047 .12Enzymes involved in DNA synthesis7 .13The glycogen particle7 .14ConclusionsReferencesChapter 831 531 531 731 7Enzymes in the cell32 18 .1Introduction8 .2Intracellular compartmentation8 .3Compartmentation of metabolic pathways8 .4Vectorial organization of enzymes associated with membranes8 .5The concentrations of enzymes and substrates in vivoConclusions 36 68 .6ReferencesChapter 932 132 233 334 735 536 7Enzyme turnover37 09 .1Introduction9 .2Kinetics of enzyme turnover9 .3Methods for measurement of rates of enzyme turnover9 .4Results from measurements of rates of enzyme turnover9 .5Possible correlations between the rates of turnover and the structure an dfunction of enzymes 37 69 .6The mechanisms of protein degradation9 .7The significance of enzyme turnover9 .8Other processes in which intracellular proteolysis is importantConclusions 39 69 .9References37 137337 537 738 939 6Appendix 9 .1Chapter 1037 039 9Clinical aspects of enzymology40 010 .1Introduction10 .2Determination of enzyme activities for clinical diagnosis40 040139 4
10 .3Clinical enzymology of liver disease10 .4Clinical enzymology of heart disease10 .5Other enzyme activities that become elevated in serum in diseaseThe detection and significance of enzyme deficiencies 41 110 .640 8Enzyme inhibitors and drug design10 .8The use of enzymes to determine the concentrations of metabolites o fclinical importance 41 610 .9Enzyme therapy10 .10ConclusionsChapter 1141 542442 842 8Enzyme technology43 011 .1Introduction11 .2Use of microorganisms in brewing and cheesemaking11 .3Use of microorganisms in the production of organic chemicals11 .4Use of isolated enzymes in industrial processes11 .5Immobilized enzymesReferences43 044 0449Appendix : Enzymes referred to in Chapters47140 610 .7ReferencesIndex4041-11 45043 343 043 3
The Cell and Molecular Biology of Catalytic Proteins THIRD EDITION Nicholas C. Price Lewis Stevens. A note on units xv List of abbreviations xvi i Chapter 1 Introduction 1 1 .1 Aims of this book 1 1 .2 Historical aspects 1 1 .3 Remarkable properties of enzymes as catalysts 4 1 .4 Cofactors 7
Liljeruhm, J. 2019. Exotic Ribosomal Enzymology. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1770. 43 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0567-7. This thesis clarifies intriguing enzymology of the ribosome, the multiRNA/multiprotein
Methods in Enzymology Volume 148 Plant Cell Membranes EDITED BY Lester Packer Roland Douce DEPARTMENT OF PHYSIOLOGY AND ANATOMY DEPARTEMENT DE RECHERCHE FONDAMENTALE UNIVERSITY OF CALIFORNIA CENTRE D'ETUDES NUCLEA1RES ET UNIVERSITE BERKELEY, CALIFORNIA DE GRENOBLE GRENOBLE, FRANCE ACADEMIC PRESS, INC. Harcourt Brace Jovanovich, Publishers
Introduction to Enzymology. Enzymes - Biological catalysts By definition a Catalyst : - Accelerates the rate of chemical reactions . physical methods to solve structure of enzymes Conformation with or without substrate provides functional/biological information Used to identify amino acids involved
Methods in Enzymology Volume LV Biomembranes Part F: Bioenergetics-Oxidative Phosphorylation EDITED BY Sidney Fleischer DEPARTMENT OF MOLECULAR BIOLOGY VANDERBILT UNIVERSITY, NASHVILLE, TENNESSEE Lester Packer MEMBRANE BIOENERGETICS GROUP DEPARTMENT OF PHYSIOLOGY-ANATOMY
Rossmann, Methods in Enzymology 1985, p. 242 Optronics P-1000 film scanner Photo provided by Dieter Schneider, Brookhaven National Laboratory Ron Hamlin - Supper Award talk Diffractometer: automatic but measured only one hkl reflection at a time Robert Sweet, Methods in Enzymology 1985 Ron Hamlin - Supper Award talk
Computational enzymology: modelling the mechanisms of biological catalysts -4- ibuprofen) demonstrate the potential of QM/MM methods to deal with practical questions of drug metabolism22. Cytochrome P450 is an enzyme which is important in drug metabolism. This picture shows QM/MM modelling of cytochrome P450.
References: Methods in Enzymology 100, 468-500 (1983) describes the use of M13 vectors. Methods in Enzymology 154, 329-50. These references and additional references within the volumes describe the original procedures for making mutants. If you know nothing about cloning of genes, use of M13 phage and their life cycle, this is a good place to .
of numerical simulation. These methods are widely applicable to more complex systems of reversible modification. 2. Results 2.1. Enzymology of modification and demodification We discuss the enzymology of reversible modification in gen-eral and specialise to the case of the GK loop in subsequent sections.
