GC Columns Zebron Inferno

GC ColumnsZebron Inferno Rugged GC Performance to 430 !4 Versatile Selectivities ZB-1HT ZB-5HT ZB-35HT ZB-XLB-HTwww.phenomenex.com/InfernoGC

Introducing the Zebron ExperienceGet More Than Just A ColumnWhen you choose Zebron, you get more than just high qualityGC products. Choosing Zebron means you get access to a widevariety of tools, resources, and personalized support to help makeyour GC work easier, faster, and simply better.Our CustomersCome First“Phenomenex has always given superbcustomer support. I changed suppliers from Agilent to Phenomenex based on yourcustomer support about 10 years ago andyou have not failed me since that point !“Marie CoschiganoGenzyme Corp. USAContinued InnovationOur inventive GC scientists have 25 years of experience, and many helped create keystone phases at J&W Scientificbefore joining the Phenomenex team. Zebron’s track record of innovation is the only to be recognized with 3 R&D 100Awards and 2 Readers’ Choice awards!ZB-WAXPLUS 100 % water stableBirth of Zebron GCColumns19982004MS Phases2005ZB-1XT SimDistRobust simulated distillationInferno PhasesStable up to 430 C20062007ZB-MultiResidue PhasesTargeted selectivity forpesticide screening2009ZB-Drug-1For drugs of abuseZB-Bioethanol5 min resultsThe opinions stated herein are solely those of the speaker and not necessarily those of any company or organization. 2015 Phenomenex, Inc. All rights reserved.2PhenomenexlWEB: www.phenomenex.com2010ZB-CLPesticides PhasesVersatility for pesticides byGC/ECD20122014ZB-SemiVolatilesSupreme inertness forSVOCs, PBDEs, and PAHs

Zebron Inferno For Rugged, High Temperature GC Performance Up to 430 04 ------------------ Overcome Traditional Obstacles05 ------------------ High Temp Performance: How It Works06 ------------------ Extremely Low Bleed07 ------------------ Increase Column Lifetime08 ------------------ Separate High Boiling Compounds With Ease10 ------------------ Bake Off Contaminants, Eliminate Carryovers11 ------------------ See What You’ve Been Missing!12 ------------------ Explore Versatile Selectivities15------------------ Featured l22Food & Beverage23Industrial Chemical24Pharmaceutical2512------------------ Ready to ded GC Accessories25PhenomenexlWEB: www.phenomenex.com3

Overcome Traditional ObstaclesChallenge # 1: Short Fused Silica Column LifetimesUnlike Zebron Inferno GC columns, traditional fused silica columns are not equipped to handle prolonged exposure totemperatures above 360 C. At high temperatures, fused silica GC columns: Become inflexible and brittleBreak easily and cause costly replacementRequire more system downtime for maintenanceCan’t separate high molecular weight compoundsDeterioration of a standard columnat high temperaturesChallenge # 2: Cumbersome Metal Column TubingThough metal columns have higher temperature limits than capillary columns, they are more difficult to use.Most metal columns: 4Are inflexible and hard to useRequire special tubing cutters for installationAre prone to leaks from expansion and contractionHave active surfaces, giving poor peak shapes for acids and basesAre incompatible with MS detectorsPhenomenexlWEB: www.phenomenex.com

Better High Temp Performance: How It WorksZebron Inferno columns offer enhanced performance where other columns fail. The two critical factors that allow for this are:1. Advanced ESC Bonding Technology SpeciealHighTeginolyimide CoaePtturraSepused ilicaFmonary Phaa tisStAt high temperature ranges, the stability of standard GCcolumns deteriorates, resulting in increased bleed. Zebron’sEngineered Self Cross-linking (ESC) bonding technologyreinforces the stationary phase for enhanced column durabilityand extremely low bleed levels at high temperatures. Theresult: Inferno columns with the flexibility to perform for hightemp oven ramps and bakeouts.2. High Temperature Polyimide CoatingStandard polyimide resin pyrolyzes at temperatures above360 C, making the tubing unstable. Zebron Inferno columnsuse a temperature resistant polyimide resin that shows minimalthermal degradation, even at temperatures up to 430 C*. Thismeans longer column lifetime at elevated temperatures.Inferno PhaseTemperature LimitZB-1HT430 CZB-5HT430 CZB-35HT400 CZB-XLB-HT400 CFlexible Even at 430 C!**Zebron Inferno columns remain flexibleand easy to work with, even after beingexposed to many hours at extremetemperatures. This stability offers theflexibility of a non-metal column whileproviding long lifetimes, low bleed, andlow activity.* Zebron ZB-1HT and ZB-5HT Inferno columns have an upper temperature limit of 430 C.Zebron ZB-35HT and ZB-XLB-HT Inferno columns have an upper temperature limit of 400 C.** Evaluated by performing 185 programmed temperature runs, total 23 hours at 430 C.Polyimide tubing was still flexible as shown here.PhenomenexlWEB: www.phenomenex.com5

Extremely Low Bleed for Rugged Performance Longer column lifetimeBetter reproducibilityMaximum sensitivityMore accurate quantitationWhat is column bleed?Column bleed is the loss of stationary phase (MS ions 355, 281, 207, and 73) as a result ofimpurities in the starting polymer or the decomposition of the phase at elevated temperatures.Why is column bleed undesirable?Column bleed can be an indicator of the stability and lifetime a GC column will offer. Whenusing sensitive detectors such as MS, bleed can impact method detection limits (MDLs) causingdifficulties at the low calibration ranges.What sets Zebron’s bleed apart from others?Our proprietary ESC bonding technology and special polyimide tubing enhance columndurability for extremely low bleed levels at high temperature.Zebron Inferno Columns Have Lower Bleed25400 CConditions for all columns:Dimensions: 30 meter x 0.25 mm x 0.10 μmInjection: Null Injection @ 250 CCarrier Gas: Hydrogen @ 11 psi (constant pressure)Oven Program: 120 C for 3 min to 320 C @ 30 C/min (hold 5 min) to2321Low bleed at hightemperature!340 C @ 30 C/min (hold 5 min) to 360 C @ 30 C/min (hold 5 min) to380 C @ 30 C/min (hold 5 min) to 400 C @ 30 C/min (hold 5 min)Detector: FID @ 405 C19380 CpA1715Zebron ZB-5HT Inferno13Agilent DB -5ht119The shaded area depicts the bleed criteria for MScertified columns on a MS detector. MS bleed certification values are typically read at 320 ºC. ZB-5HTmeets MS certification limits even at 360 ºC!75051015Comparative separations may not be representative of all applications.6PhenomenexlWEB: www.phenomenex.com20253035min

Increased Column Lifetime with Enhanced Durability Less instrument downtime More productivity Lower laboratory costsStandard GC Columns Cannot Withstand High TemperatureAfter prolonged exposure to temperatures above 360 C, most traditional GC columns become brittle and inflexible, oftenspontaneously breaking in the middle of a run. Work is stopped in order to change out the column and perform system maintenance.Unlike these fragile columns, Zebron Inferno columns have a thermally resistant polyimide coating for longer column lifetime attemperatures up to 430 C. As a result, you get less instrument downtime, and higher productivity.TMZebron Inferno Columns8.5Win In The Lifetime TestOver 2x the Lifetimevs. VF-5ht!Conditions for all columns:Dimensions: 30 meter x 0.25 mm x 0.10 μmInjection: 1.0 μL of test mix AG0-7578Carrier Gas: Helium @ 1.9 mL/min (constant flow)Oven Program: 120 C IsothermalDetector: Flame Ionization Detector (FID) @ 400 CSample: Pentadecane8Retention of Pentadecane at 120 C (min)TM7.57Agilent DB -5htAgilent VF-5htZebron ZB-5HT6.56Comparison of pentadecane retention between Zebron ZB-5HT Inferno and other 5 % phenyl 95 % dimethylpolysiloxane columns.Note that the VF-5ht died around 40 hours at 400 C whereas ZB-5HT Inferno maintained great retention of pentadecane for over 100hours - over 2x the lifetime!020406080100120Hours at 400 CComparative separations may not be representative of all applications.How does the lifetime test work?Hydrocarbons are a good way to measure the stability and lifetime ofa non-polar column. Because its interaction with the phase is mostlybased on London Dispersion forces, any change in retention time iscorrelated with phase loss. This will result in increased bleed and poorreproducibility.In the lifetime study above, the Zebron ZB-5HT Inferno lasts twice aslong as competing manufacturers. For the test, all columns were heldat 400 C for 2 hours and then the oven was lowered to 120 C forpentadecane analysis. The Agilent VF-5ht column broke just after 40hours at 400 C. The Agilent DB-5ht column had the same retention forpentadecane at 40 hours as the ZB-5HT at 100 hours.PhenomenexlWEB: www.phenomenex.com7

Greater Performance for High Boiling Compounds Better peak shape Improved separation More analytes detectedZebron Inferno columns have the inertness and temperature stability needed to separate high molecular weight compounds. Thesehigh boilers require a thermally rugged column with low bleed levels. Inferno columns provide optimal separation of these analytes.GreatSeparation of High Boiling Hydrocarbons (ASTM Method D6352)1614846 7 98 10 1211 13141516 1817 1921027 2921 23 2520 22 24263510Easily Elute HydrocarbonsUp To C903331283032373836 39 413540 42434445344647484956 5850 5254 5751 555315Column: Zebron ZB-1HT InfernoDimensions: 5 meter x 0.53 mm x 0.10 μmPart No.: 7AK-G014-02Injection: On-Column @ 43 C, 0.1 μLCarrier Gas: Helium @ 4.4 mL/min (constant flow)Oven Program: 40 C for 0.5 min to 430 C @ 20 C/min for 10 minDetector: FID @ 430 CNote: Sample was a combination of POLYWAX 655 andretention time markers C8-C40 in CS2/Chloroform8PhenomenexlWEB: www.phenomenex.com20Sample:App ID 161485Zebron ZB-1HT Inferno can operateup to 430 C, allowing C90 to eluteon temperature ramp to meet ASTMrequirements!min1. C102. C113. C124. C135. C146. C157. C168. C179. Pristane10. C1811. Phytane12. C1913. C2014. C2115. C2216. C2317. C2418. C2519. C2620. C2721. C2822. C2923. C3024. C3125. C3226. C3327. C3428. C3529. C3630. C3731. C3832. C3933. C4034. C4235. C4436. C4637. C4838. C5039. C5240. C5441. C5642. C5843. C6044. C6245. C6446. C6647. C6848. C7049. C7250. C7451. C7652. C7853. C8054. C8255. C8456. C8657. C8858. C90

16530Sterols From Margarine by GC/MS on ZB-5HT Inferno 4314.204.404.604.80Column:Dimensions:Part No.:Injection:Carrier Gas:Oven Program:Detector:Sample:5.00App ID 1653025.205.405.605.806.006.20minZebron ZB-5HT Inferno30 meter x 0.25 mm x 0.10 µm7HG-G015-02Splitless @ 275 C, 0.5 µLHelium @ 1.5 mL/min (constant flow)220 C to 350 C @ 20 C/minMSD @ 275 C1. Campesterol2. Stigmasterol3. b-Sitosterol4. Betulin (IS)PhenomenexlWEB: www.phenomenex.com9