Axygen Gel Documentation System And Axygen Gel .

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Axygen Gel Documentation System andAxygen Gel Documentation System-BLUser ManualA Corning Brand

Safety and Regulatory ComplianceImportant Safety InformationPlease read these instructions before operating the Axygen Gel Documentation System (GD-1000) andAxygen Gel Documentation System-BL (GDBL-1000).UV Safety PrecautionsUVCAUTIONBecause ultraviolet (UV) radiation can cause seriousdamage to unprotected eyes and skin, we recommendwearing UV glasses or a face shield.The Axygen Gel Documentation systems come with a built-in ultraviolet (UV 200 to 400 nm)transilluminator inside the system. Exposure to UV radiation can cause permanent damage to the eyes andskin. The system enclosure confines the radiation within the system and shields the user from exposure. Thesystem is also equipped with a two-way safety interlock switch that automatically cuts off the power to thetransilluminator when the door is open during normal use. Always wear UV-protective eyewear that is specified by the manufacturer as providing protection at thewavelength(s) used, making sure that the eyewear protects any areas where radiation may come through(UV sunglasses may not prevent UV radiation from coming in through the sides or around the lenses). Always cover all skin that may be exposed to UV light, especially the face, neck, hands, and arms. Always make sure that any UV protection devices (such as the safety switch on the light cabinetapparatus) are working properly. If not, discontinue use until the device(s) are properly repaired. Use only UV lamps in the transilluminator.Electrical Safety PrecautionsBe sure to take proper precautions when handling any electrical equipment. NEVER work on any live circuit,fixture, receptacle, or switch. Safety rules you should follow whenever working with any electrical applianceinclude: Always shut off power at the main disconnect before changing a fuse. Always shut off power to the circuit before repairing or replacing a switch, receptacle, or fixture. Always tape over the main switch, empty fuse socket, or circuit breaker you are working on. Always check that the circuit is dead before beginning work on it. Using a circuit tester or voltmeter canhelp you determine this. Always unplug any appliance before repairing it.Protective Earth Terminal he earth terminal, intended for connection to an external protective conductor for protection againstTelectric shock in case of a fault, is located on the inside of the back panel.Hot Surface WarningUnder normal conditions, the temperature of glass surface of UV transilluminator is below 50 C and safe totouch. However, if the system malfunctions, it is possible that the glass surface temperature exceeds 80 C.Please exercise caution when touch the glass surface with your hand when this occurs.IIVisit www.corning.com/lifesciences for additional product and technical information.

For General Laboratory Use OnlyThis instrument is suitable for general laboratory use only. Therefore, it must be used only by personnel whoknow the health risks associated with the reagents that are normally used with this instrument.Voltage Setting InformationThe Axygen Gel Documentation systems have a power supply that automatically chooses the correctvoltage for your country or region.Protection category: IP20 according IEC 60529For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.1

Table of Contents1. Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32. Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43. System Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54. Axygen Gel Documentation System Installation Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65. Axygen Gel Documentation System-BL Installation Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86. Image Acquisition. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97. Overview of Software Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108. Optional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139. Copyright and Trademark Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1410. Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1511. Waste Electrical and Electronic Equipment (WEEE) Directive . . . . . . . . . . . . . . . . . . . . . . . . . . . 162Visit www.corning.com/lifesciences for additional product and technical information.

1. IntroductionCongratulations on purchasing your Axygen Gel Documentation System or Axygen Gel DocumentationSystem-BL. The systems easily capture publication quality, 16 bit TIFF images. The systems are simple toset up and have a straightforward user interface for image capture, annotation, and contrast adjustment.Images are easily saved and opened in common gel analysis software for more detailed analysis.The Axygen Gel Documentation System includes the following components: Axygen Gel Documentation system cabinet, including the camera and orange filter A-to-A male USB cable Quick Installation Guide and Quick Imaging Guide Axygen test target Axygen USB (includes imaging software, calibration files) 3-power cable 110V/220V (US/EU/UK). Please select the correct one for your region.The Axygen Gel Documentation System-BL includes the following components: Axygen Gel Documentation System-BL cabinet, including the camera and orange filter Tablet computer (includes imaging software, calibration files) Quick Installation Guide and Quick Imaging Guide Axygen test target 3-power cable 110V/220V (US/EU/UK). Please select the correct one for your region.For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.3

2. SpecificationsAxygen SystemGel Documentation SystemGel Documentation System-BLCamera5.4 MP image resolution5.4 MP image resolutionLensF1.8F1.8Light Sources Epi White Epi White Dual wavelength transillumination at 302 nm Dual wavelength transillumination at 302 nm andand 365 nm365 nm Epi BlueTrans White LightIllumination TrayOptionalOptionalField of View15 x 20 cm15 x 20 cmCabinet Door sensor to prevent accidental exposure to Door sensor to prevent accidental exposure to UV;UV; automatically shuts off UV light when dooris open Safety override, enabling band cutting on theUV transilluminator with automatic turn offafter 5 minutesautomatically shuts off UV light when door is open Safety override, enabling band cutting on the UVtransilluminator with automatic turn off after 5minutesEmission Filter590 nm590 nm3-position Filter SliderNoYesCertificationsCE, cTUVusCE, cTUVusProduct Footprint12” x 15” (30.48 x 38.1 cm)12” x 15” (30.48 x 38.1 cm)Computer Operating system: Microsoft Windows 7Tablet included: Windows 8 tablet computer enablescompatibility with existing networks, as well aseasy setup (also Windows 10 compatible)and above Minimum hardware requirements: 1.4 GHzprocessor speed, 2 GB RAM, 16 GB free hard diskspace, 2 USBs Bluetooth and wireless compatible, or USB to LANcompatible 10.1" touch screen 2 GB of storage for short term data saving4Visit www.corning.com/lifesciences for additional product and technical information.

3. System PlacementPlease make sure the system is located away from regular exposure to water, solvents, or corrosive material.Place on a flat and stable surface with 10 cm clearance on all sides, including the top, to allow sufficient airflow.The system is intended for indoor use with the following ambient conditions:a. Altitude up to 2,000 mb. Temperature 5 C to 40 Cc. Maximum relative humidity 80% for temperatures up to 31 C decreasing linearly to 50% relativehumidity at 40 CThe system should be placed away from interfering electrical signals and magnetic fields. If possible, adedicated electrical outlet should be used to eliminate electrical interference from other instrumentation inyour laboratory.For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.5

4. Axygen Gel Documentation System Installation Guide1.2.3.4.On the USB, find the folder“Axygen Imaging Files\AxygenGDS v XXX” and open it.Double click on the Setup file.Remove the Axygen GelDocumentation system fromits packaging.Identify the Axygen USB andconnect it to a Microsoft Windows 7 or 8 computer.(You may see a message popup that says “Do you want toallow the following programfrom an unknown publisherto make changes to thiscomputer?” Select Yes.)Click Next to begin the SetupWizard.5.6.7.8.Select GD1000, then click Next.To continue, accept the licenseagreement. Then click Next.Select your installationlocation, then click Next.Click Install to continue withthe installation.9.10.11.12.Click Next to begin the driverinstallation.To continue, accept the licenseagreement. Then click Next.After the installation iscomplete, click Finish.Click Finish to exit the SetupWizard.6Visit www.corning.com/lifesciences for additional product and technical information.

4. Axygen Gel Documentation System Installation Guide (continued)13.14.15.16.Plug the system into anavailable outlet.Connect the A-to-A USB cableto both the system USB andthe computer. On the Axygensystem, ensure the USB isplugged into the lower USBon the right hand side ofthe instrument.Find the “Master” folderwithin the “System CalibrationFolder.” Copy the Masterfolder, and paste it inC:\ProgramData\Axygen\Axygen Imaging.When asked if you would liketo replace the existing folder,select Yes.On the Axygen USB, locate thefolder “Axygen Imaging Files\System Calibration FolderAXXXXX.”* Note: if you cannot see theProgram Data folder, pleasemake sure you can view“hidden” files in Windows .17.18.19.Power on the Axygen GelDocumentation system usingthe switch in the back. Waitabout 20 seconds beforeproceeding to the next step.Launch the capture software.The first time you run theAxygen Capture Software,you will receive the messageabove. Click Allow Access.For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.7

5. Axygen Gel Documentation System-BL Installation Guide1.2.3.4.Remove the Axygen GelDocumentation system-BLfrom its packaging.Locate the tablet computer.Remove the wing nuts fromthe back of the tablet.Mount the tablet on thesystem.5.6.7.8.Secure the tablet in place withthe wing nuts.Connect the mini-USB cable tothe tablet.Connect the power cable tothe tablet.Plug the system into anavailable outlet.9.10.11.Power on the system using theswitch on the back.Power on the tablet.Launch the capture software.8Visit www.corning.com/lifesciences for additional product and technical information.

6. Image Acquisition1. After the acquisition software is launched, select the Darkroom tab.2. Place your sample in the imager. The capture software will be in “Live” mode to allow you to positionyour sample.3. Close the cabinet door.4. Select your light source.Here is a quick guide to select your light source:Axygen SystemGel Documentation SystemGel Documentation System-BLEtBr GelUV 302 or 365UV 302 or 365Sybr Safe GelUV 302Epi BlueCoomassie Gel*VisibleVisible*Requires optional white light tray5. Select exposure time. If you are not sure of your exposure time, select Auto Exposure. Otherwise, youcan select a time using the exposure slider.6. A preview of your image will be displayed. If the image is acceptable, select Image Capture. Otherwiseyou can continue to modify the exposure time.7. When you are done, your image will be displayed in the home tab. You can now save your image.For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.9

7. Overview of Software FeaturesHome TabOpenOpen a previously saved image.SaveiImage InfoCaptures some of the information on how yourimage was captured, including the date andtime captured, and exposure time. Additionally,it tells you the light source and calibration value.Save allows you to perform one of three actions:Zoom In Save – save the currently active imageZoom in on center of image Save as – allows you to save the currentlyactive image under another file name Save all – saves all open imagesZoom OutZoom out from center of imagePrintPrint active image.Zoom Fit@EmailAllows you to email the active image. Please see“Email Settings” in the Settings section of thisguide.Adjusts zoom to fill window. Displays % in thelower left corner of the window.Auto ContrastResizeCalculates a balanced contrast ratio.An image can be resized up to 3 times itsoriginal size.Manual ContrastCropWhen you select the crop tool, a crop windowwill appear over your image. Drag the corners ofthe crop window to your desired size, and thenselect “Crop ROI” from the bottom of the screen.If you do not want to crop, select “Cancel.”Allows you to manually adjust the black andwhite levels of your image.InvertGives the inverse of the image.RotateRotate allows you to rotate your image one offour ways: Right 90 degreesDisplay SaturationSaturated pixels are displayed in red. Left 90 degrees Flip horizontal Flip vertical10Visit www.corning.com/lifesciences for additional product and technical information.

7. Overview of Software Features (continued)Darkroom TabUV 302Auto ExposureSelects the Trans UV 302 light source.Calculates an exposure time using the fulldynamic range of the camera.UV 365Show GridSelect the Trans UV 365 light source.Displays a grid over the live image so you canmake sure your gel is vertically and horizontallystraight.Epi WhiteSelects the Epi White light source.Select ROISelect ROI has two functions: Select ROI then select Image Capture – thisEpi Blueresults in a final cropped imagedTurns on the Epi Blue light source.Axygen Gel Documentation System–BL only Select ROI then select Auto Exposure – thisresults in auto exposure calculations onlyinfluenced by your region of interest.Trans WhiteImage CaptureOptional – with the purchase and calibration ofthe white light tray. You select the Trans Whitelight source.Select this to capture your image. It will bedisplayed in the Home tab for further work.Band ExcisionSliderUse this slider to select your exposure time. As you changethe time, wait for the live image below to update for the bestexperience. Alternatively, you can type in the exposure time inthe text fields to the right, and then select Enter.If you need to open the door and view your gelwith the lights on, you can override the safetyprecautions in our system.NOTE: Always wear protective eyewear whendealing with UV light. To use the band excisiontool, select your light source then select bandexcision. A dialog box will appear and ask youto pull out the safety switch on the right sideof the system. Open the door, pull the safetyswitch, and the light will go on. Select “BandExcision” again to turn the light off. The light willautomatically turn off after 5 minutes.For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.11

7. Overview of Software Features (continued)Annotations TabThe annotations tab lets you annotate or draw on an open image.PointerPencilThis is the object selector. It allows selection oftext boxes, objects, lines, etc., in order to edit ordelete them. For example, to change the color ofa box, select the pointer, double click on the boxto be changed, then select the color select tooland click on the color desired.Use this to free-hand draw on the image.UndoUse this tool to undo your last few steps.RectangleDraw a rectangle on the imageRedoRedo your last step.EllipseDraw an ellipse on the imageDeleteDelete the highlighted annotation object.LineDraw a line on the imageFont/Color/Line WidthOnce an annotation object is selected usingthe pointer, you can edit the line width and thecolor, and in the case of text, the font.TextClick on the text box, then draw a rectangle. Thisrectangle will be where your text appears. Youcan change the size of the font after you typeyour text.Settings TabThe password for settings is admin.@Email SettingsCreate FlatsYou can email files to yourself if you set up anemail account for the imaging system. Contactyour IT department for the best values to set.This tool is used to create flat field imagefor certain light sources. Flat files are usedto calibrate light sources for the most evenillumination. Flat field calibration is requiredif you purchase the White Light Tray accessory.Instructions for calibration and use are includedwith the tray.Change PasswordThe default system password is admin (alllowercase). If you would like to change thispassword, you can do so here.Enable Trans WhiteWhen optional White Light Tray is purchased,enables Trans White to appear as an option inthe darkroom.Help TabAllows you to view this user manual, save it, or print it.12Visit www.corning.com/lifesciences for additional product and technical information.

8. Optional AccessoriesCorning Cat. No.DescriptionGD-1000Gel Documentation SystemGDBL-1000Gel Documentation System-BLGD-BLCSGel Documentation System blue light conversion screen for GD-1000GD-WLCSGel Documentation System white light conversion screen for GD-1000and GDBL-1000GD-GCTGel carrying tray for GD-1000 and GDBL-1000GD-PRTThermal printerGD-PAPERThermal printer paperGD-1D1 user license of Total Lab 1D (also available in more user licensesupon request)GD-1DN1 user Network license of Total Lab 1D (also available in more user licensesupon request)GDBL-BLUEOptional blue filter (497 nm)GDBL-GREENOptional green filter (572 nm)GDBL-REDOptional red filter (676 nm)GD-GCSUV transmitting tray that protects UV transilluminator when cuttingout bandsGD-OGOrange glasses ideal for reducing background when cutting out bands withblue lightGD-USBSUSB hubGD-USBMKUSB mouse and keyboardFor more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.13

9. Copyright and Trademark InformationAll goods and services are sold subject to the terms and conditions of sale of the company that suppliesthem. The company reserves the right, subject to any regulatory and contractual approval, if required, tomake changes in specifications and features shown herein, or discontinue the product described at any timewithout notice or obligation.Corning IncorporatedLife Sciences836 North St.Building 300, Suite 3401Tewksbury, MA 01876t 800.492.1110t 978.442.2200f 978.442.2476www.corning.com/lifesciencesFor more specific information on claims, visit the Certificates page at www.corning.com/lifesciences.Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not intendedfor use in diagnostic or therapeutic procedures. Corning Life Sciences makes no claims regarding theperformance of these products for clinical or diagnostic applications.14Visit www.corning.com/lifesciences for additional product and technical information.

10. WarrantyThe Axygen products are warranted against defects in materials and workmanship for two years unlessotherwise outlined on your sales order. If any defect occurs in the instrument during this warranty period,Corning will repair or replace the defective parts at its discretion without charge. However, the followingdefects are specifically excluded: Defects caused by imp

Locate the tablet computer. Remove the wing nuts from the back of the tablet. Mount the tablet on the system. Secure the tablet in place with the wing nuts. Connect the mini-USB cable to the tablet. Connect the power cable to the tablet. Power on the system using the switch on the back. Power on the tablet. Launch the capture software.

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