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Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous EnvironmentMultiple Choice Questions1) Which of the following represents the breaking of a noncovalent interaction?A) hydrolysis of an esterB) dissolving of salt crystalsC) ionization of waterD) decomposition of hydrogen peroxideE) none of the aboveAnswer: BDifficulty: 1Topic: The Nature of Noncovalent Interactions2) Which of the following is the most likely noncovalent interaction observed between acarboxylic acid and an alcohol?A) charge-charge interactionB) charge-dipole interactionC) dipole-dipole interactionD) dipole-induced dipole interactionE) formation of an ester bondAnswer: BDifficulty: 1Topic: The Nature of Noncovalent Interactions3) Which of the following atoms could interact through a hydrogen bond?A) the oxygen of a ketone and the hydrogen of an aldehydeB) the oxygen of methanol and a hydrogen on the methyl carbon of methanolC) the hydrogen of an amine and the oxygen of an alcoholD) the hydrogen on an aromatic ring and the oxygen of carbon dioxideE) none of the aboveAnswer: CDifficulty: 2Topic: The Nature of Noncovalent Interactions1Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment4) Which of the following would likely form micelles in an aqueous solution?A) hexaneB) glucoseC) glutamic acidD) dodecanoic acidE) none of the aboveAnswer: DDifficulty: 2Topic: The Role of Water in Biological Processes5) Which of the following best explains the hydrogen bonding that occurs in water?A) the average number of H-bonds formed by one water molecule is the same in liquid andsolid water, the only difference is the duration of the H-bondB) the number of H-bonds formed by one water molecule is greater in liquid water than insolid waterC) the structure of liquid water is best described as flickering clusters of H-bonds due to therelative short duration of individual H-bondsD) each water molecule is capable of forming 8 H-bonds: 2 from each lone pair of electronsand 2 from each hydrogenE) none of the aboveAnswer: CDifficulty: 2Topic: The Role of Water in Biological Processes6) Given the structure of a glucose molecule, which of the following explains the hydrogenbonding between glucose and water?A) H-bonds will form with glucose always being the H-bond donorB) H-bonds will form with water always being the H-bond donorC) each glucose molecule could H-bond with as many as 17 water moleculesD) due to the cyclic structure of glucose, H-bonding with water does not occurE) none of the aboveAnswer: CDifficulty: 2Topic: The Role of Water in Biological Processes2Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment7) Which of the following acids or bases is least likely to be encountered in a biochemicalsetting?A) nitric acidB) acetic acidC) ammoniaD) citric acidE) phosphoric acidAnswer: ADifficulty: 1Topic: Ionic Equilibria8) What pH range is generally considered to be the physiological pH range?A) 1.5-3.0B) 4.5-7.0C) 5.5-9.0D) 6.5-8.0E) 7.5-10.0Answer: DDifficulty: 1Topic: Ionic Equilibria9) Since pKa -log Ka, which of the following is a correct statement?A) acetic acid (pKa 4.7) is stronger than lactic acid, (pKa 3.9)B) lactic acid, (pKa 3.9) is weaker than all forms of phosphoric acid, (pKa 2.1, 6.9 and12.4)C) since the pKa for conversion of the ammonium ion to ammonia is 9.25, ammonia is aweaker base then the acetate ionD) for carbonic acid with pKa values of 6.3 and 10.3, the bicarbonate ion is a stronger basethan the carbonate ionE) none of the aboveAnswer: EDifficulty: 2Topic: Ionic Equilibria3Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment10) If gastric juice has a pH of about 1.5, which of the following would be predominantlydeprotonated in the stomach?A) phenol (pKa 9.9)B) acetic acid (pKa 4.7)C) lactic acid, (pKa 3.9)D) phosphoric acid (pKa 2.1)E) hydrochloric acid (pKa -6)Answer: EDifficulty: 2Topic: Ionic Equilibria11) Given the pKa values for phosphoric acid of 2.14, 6.86 and 12.4, what is the ratio of HPO42/H2PO4- in a typical muscle cell where the pH is 7.2?A) 0.46B) 2.2C) 6.3 10-6D) 1.1 105E) none of the aboveAnswer: BDifficulty: 2Topic: Ionic Equilibria12) You have been asked to determine the pKa of an unknown acid. In a solution at pH 7.0, youfind that 24% of the acid is in its deprotonated form. What is the pKa of the acid?A) 7.9B) 7.5C) 6.5D) 5.6E) none of the aboveAnswer: BDifficulty: 2Topic: Ionic Equilibria4Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment13) Citric acid is a triprotic acid with three carboxylic acid groups having pKa values of 3.1, 4.8,and 6.4. If a solution of citric acid has a pH of 5.5, what can be said about the predominantprotonation state of the citric acid?A) 1 carboxylic acid group is deprotonated, 2 are protonatedB) 2 carboxylic acid groups are deprotonated, 1 is protonatedC) all 3 carboxylic acid groups are deprotonatedD) all 3 carboxylic acid groups are protonatedE) the protonation state cannot be determinedAnswer: BDifficulty: 2Topic:Ionic Equilibria14) Which of the following is the conjugate acid of hydrogen phosphate, HPO42-?A) H2PO3B) H2PO4C) H3PO4D) H2PO42E) none of the aboveAnswer: BDifficulty: 1Topic: Ionic Equilibria15) Formic acid is the active agent in an ant bite. What is the ratio of base/acid for formic acid(pKa 3.9) in the blood stream at pH 7.4?A) 3.16 10-4B) 3.16 103C) 3.5D) 0.54E) 1.90Answer: BDifficulty: 1Topic:Ionic Equilibria5Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment16) If a buffer is made with the pH below the pKa of the weak acid, the ratio of [base]/[acid] willbe:A) less than 1B) greater than 1C) equal to 1D) equal to 0E) cannot be determinedAnswer: ADifficulty: 1Topic:Ionic Equilibria17) A typical amino acid has a carboxylic acid and an amine with pKa values of 2.3 and 9.6,respectively. In a solution of pH 4.5, which of the following best describes the protonationand charge state of the amino acid?A) carboxylic acid:B) carboxylic acid:C) carboxylic acid:D) carboxylic acid:E) carboxylic acid:protonated and neutral; amine: deprotonated and negativeprotonated and neutral; amine: protonated and neutraldeprotonated and negative; amine: protonated and neutraldeprotonated and negative; amine: protonated and positivedeprotonated and negative; amine: deprotonated and neutralAnswer: DDifficulty: 2Topic:Ionic Equilibria18) Glutamic acid contains two carboxylic acid groups (pKa values of 2.2 and 4.2) and an aminegroup (pKa 9.7). What is the pI for glutamic acid?A) 3.2B) 6.0C) 6.5D) 7.0E) none of the aboveAnswer: ADifficulty: 2Topic:Ionic Equilibria6Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment19) Lysine contains two amine groups (pKa values of 9.0 and 10.0) and a carboxylic acid group(pKa 2.2). In a solution of pH 9.5, which of the following would best describe the protonationand charge state of lysine?A) carboxylic acid: deprotonated and neutral; both amines: protonated and positiveB) carboxylic acid: deprotonated and negative; both amines: deprotonated and neutralC) carboxylic acid: deprotonated and negative; amine (pKa 9.0): deprotonated and neutral;amine (pKa 10.0): protonated and positiveD) carboxylic acid: deprotonated and negative; amine (pKa 9.0): protonated and positive;amine (pKa 10.0): deprotonated and neutralE) carboxylic acid: deprotonated and neutral; amine (pKa 9.0): deprotonated and neutral;amine (pKa 10.0): protonated and positiveAnswer: CDifficulty: 3Topic:Ionic Equilibria20) In the following titration curve, what does the inflection point represent?A) pH of solution equals pKa of weak acidB) concentration of weak acid and conjugate base are equalC) the pH where the solution would function most effectively as a bufferD) the weak acid is 50% protonated, 50% deprotonatedE) all of the aboveAnswer: EDifficulty: 2Topic: Ionic Equilibria21) What happens to DNA when placed into an aqueous solution at physiological pH?7Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous EnvironmentA) individual DNA molecules attract each other due to presence of positive and negativechargesB) individual DNA molecules repel each other due to presence of negative chargesC) individual DNA molecules repel each other due to presence of positive chargesD) DNA molecules bind to negatively charged proteinsE) none of the aboveAnswer: BDifficulty: 2Topic: Interactions Between Macroions in Solution22) What solution conditions are required for a protein to be a positively charged macroion?A) pH of solution is greater than the protein’s pIB) pH of solution is less than the protein’s pIC) pH of solution is greater than the protein’s pI and ionic strength is lowD) pH of solution is less than the protein’s pI and ionic strength is lowE) none of the aboveAnswer: BDifficulty: 2Topic: Interactions Between Macroions in Solution8Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Biochemistry 4th Edition Mathews Test BankFull file Mathews,at hews-Test-Bankvan Holde, Appling, and Anthony-Cahill, Biochemistry 4th editionChapter 2: The Matrix of Life: Weak Interactions in an Aqueous EnvironmentShort Answer Questions1) Lactic acid is a common product of actively working muscle. It is transported via thebloodstream to the liver. What percent of lactic acid is ionized in the bloodstream if the pH is7.40 and the pKa is 3.86?Answer: 99.97% (100% using correct SF)pH pKa log[lactate][lactic acid][lactate] 3467 or [lactate] 3467 [lactic acid][lactic acid][lactate] [lactic acid] 100%lactate 99.97%Difficulty: 2Topic: Ionic Equilibria 2) Imidazole is a commonly used buffer in biochemistry labs. With a pKa of 7.0, what would bethe ratio of base to acid at pH 7.4?Answer: 2.5pH pKa log[base][acid][base] 10(7.4 7.0) 2.5[acid] Difficulty: 1Topic: Ionic Equilibria3) Use the equilibrium equation of the blood buffer to predict what would happen to blood pH ifrespiration were to slow significantly.Answer: CO2 H2OH2CO3H HCO3-Slowing of respiration would cause a decrease in pH since more carbon dioxide would bepresent in the blood, forcing the equilibrium to the right, thus creating more H .Difficulty: 2Topic: Ionic Equilibria9Copyright 2013 Pearson Canada, Inc.Full file at hews-Test-Bank

Mathews, van Holde, Appling, and Anthony-Cahill, Biochemistry 4th edition Chapter 2: The Matrix of Life: Weak Interactions in an Aqueous Environment 2 Copyright .

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