Leica M-Series Stereo- Microscopes

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Leica M-SeriesStereomicroscopesUser Manual

ContentsPageOverviewSafety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Controls and functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Short instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8UseErgonomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Viewing height and interpupillary distance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Eyepoint, eye contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Changing the magnification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Focusing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Stereoscopic and axial observation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Dioptric settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Working with the stereomicroscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14AssemblyBasic outfitOverview: Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Spacer rings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Fitting the column to the incident- and transmitted-light bases . . . . . . . . . . . . . . . 18Microscope carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Binocular tubes, optics carrier, optical accessories . . . . . . . . . . . . . . . . . . . . . . . . 19Graticules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20StandsTransmitted-light stand, bright field, 20W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20Transmitted-light stands, bright and dark field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Swinging-arm stand ESD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Large swinging-arm and table-clamp stands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24IlluminatorsTransformers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26Leica L2 cold-light source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27Fibre-optic light guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Cold-light source Leica CLS Series . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28Mains (line) lamp 25W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Incident lamp 6V/10W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Incident lamp 6V/20W . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30Coaxial illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32Near-vertical illuminator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 322

PageAccessoriesFitting accessory tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33Double-iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33Drawing tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33Video-phototubes: Photography, video, TV, filming, digital imaging . . . . . . . . . . . . 34Attachment for vertical and oblique observation . . . . . . . . . . . . . . . . . . . . . . . . . . . 37Stages, polarization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37Special notesTips and hints: What to do if . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38Care and maintenance of the stereomicroscope, maintenance . . . . . . . . . . . . . . 39Calculating total magnification and field diameter . . . . . . . . . . . . . . . . . . . . . . . . . 40Optical data, MS5, MZ6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41Optical data, MZ75, MZ95 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42Optical data, MZ125, MZAPO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 443

To you, the userProhibited uses The use of the instrument in a different manner from thatdescribed in this user manual can lead to injury, malfunctionand damage. Do not fit different plugs. Do not dismantle optical systems ormechanical parts unless instructions for doing so are givenin the user manual.Thank you for your trust. We wish you success and satisfactionin your work with your new stereomicroscope from Leica.The innovative technology of the new stereomicroscope linefrom Leica is expressed externally in the avant-garde form of theinstrument. The rounded shapes, with their elliptical tendencies,demonstrate that beauty can be practical.Place of useIn developing this new stereomicroscope line we have placedgreat weight on simple, self-explanatory directions. We havehowever put a lot of effort into explaining all functions to you bymeans of pictures and text. You will only get the best out ofyour stereomicroscope if you take the trouble to study this usermanual in detail.If you still have a question sometime, just contact your localLeica agency or get in touch directly with Leica MicroscopySystems Ltd, Heerbrugg, Switzerland. We are gladly at yourservice. Customer service is a big thing with us. Not only beforethe sale, but afterwards as well.** The business units of Leica Microsystems holdthe management system certificates for the internationalstandards ISO 9001 and ISO 14001 relating to qualitymanagement, quality assurance and environmentalmanagement. The Leica MS5, MZ6, MZ75, MZ95, MZ125 and MZAPOstereomicroscopes are intended mainly for indoor use. If the stereomicroscope is used outdoors, protect it from dirtand damp. Lamps and electrically-operated stands fromLeica may not be used outdoors.Use in ESD protected roomsThe optics carrier MS5, MZ6, MZ75, MZ95, MZ125, the binoculartube, ErgoTubus , ErgoKeil 5 –25 , the swinging-arm standESD and the cold light sources Leica L2 and CLS consist of ESDdischarging material (surface resistance ( 1011 ohms/squared,discharge time 2 seconds, 1,000V to 100V). A connection socket for a 4mm dia. earthing cable isprovided on the microscope carrier. There are two connection sockets for 4mm dia. earthingcables in the base of the swinging-arm stand.ServicingRepairs may only be carried out by Leica-trained servicetechnicians. Only original Leica spare parts may be used.Safety conceptGeneral directionsBefore you try to use the instrument, please read the usermanual and the safety notes.Permitted usesThe Leica MS5, MZ6, MZ75, MZ95, MZ125 and MZAPO stereomicroscopes are optical instruments which use magnificationto see the finer details of objects close to the eye.The objects may be either natural, or created by human agency.The basic outfit, which includes a stand and a light source,can be supplemented with various modules for photography,TV, dual station viewing and other applications.4Responsibilities of person in charge of instrument Ensure that personnel who use the instrument have readand understood this user manual and in particular the safetyinstructions. Ensure that the Leica MS5, MZ6, MZ75, MZ95, MZ125 andMZAPO stereomicroscopes are operated, maintainedand repaired only by authorized and trained personnel.

Safety regulationsElectrically-operated itemsChanging bulbs Before changing a bulb, disconnect the power cable. Never attempt to touch the inside of a lamp housing ortransmitted-light stand while the instrument is connectedto the power supply. Before changing a bulb, wait until it is cool, otherwise youmay burn your fingers.The safety regulations listed below apply to the following items:– Transmitted-light stand, bright field, 20W (page 20)– Mains lamp 25W (page 28)– Regulating transformer 0–7V/40W (page 26)– Step transformer 4/5/6V (page 26)– Motor focus (separate instructions)Legal requirementsAdhere to general and local regulations relating to accidentprevention and environmental protection.LiquidsBe careful when handling liquids. If spilt on the equipment,they can:– cause the stereomicroscopes and accessories to becomeelectrically live, endangering personnel– cause damage to the equipment.Power cablesInspect cables regularly for damage. Defective cables can:Conformity with European Community directiveThe electrically-operated accessories for the Leica MS5, MZ6,MZ75, MZ95, MZ125 and MZAPO stereomicroscopes areconstructed in accordance with the latest technologies and areprovided with a statement of conformity with EC requirements.You will find these symbols in the user manual:Safety direction– directly endanger personnelThis symbol denotes information which must be readand obeyed.Failure to respect it can cause injuryFailure to respect it can cause malfunctioning,or damage to the equipment– cause the stereomicroscopes and accessories to becomeelectrically live, endangering personnel.Positioning cablesPosition cables with care. Make sure that personnel do not getentangled with them. The instrument could fall over, fall down,become damaged, damage other equipment, or cause personalinjury.Warning about accessible hot places, e.g. bulbsOpening the instrumentImportant informationOnly authorized Leica personnel are permitted to repairelectrical equipment.Before opening up the equipment, pull out the power cable.If you touch the live interior of the equipment, you may receivean electric shock.Failure to respect it can cause burnsThis symbol denotes additional informationor explanations which promote understanding.Action왘 This symbol within the text indicates that certain operationsmust be carried out.Grid voltageEnsure that the voltage setting on the equipment is correct.A wrong setting can cause damage to the equipment.ConnectionsExplanatory notes This symbol within the text stands for additional informationand explanations.When connecting power-consuming devices to the transformers, do not exceed the maximum permitted loading, as thiscan cause damage to the equipment.5

Overview6 Interchangeable objectiveVarious objectives (achromatic, planachromatic,planapochromatic), depending on the outfit.Standard outfit: Controls and functionsIn this overviewwe explain the controls and functions relating to the standardoutfits.Binocular tube7 Adjustable eyepiece tubes:Interpupillary distance adjustable within the range52mm – 76mm.Does your stereomicroscope look different from the onein the picture?8 Wide-field eyepieces for spectacle wearersThe modular design enables you to assemble an outfit matchedto the application. Controls and functions which differ fromthose in the standard outfit are described in the appropriatesection (see contents list on page 2).Leica MZAPO see page 8.9 Clamping screwsStereomicroscopeDioptric setting adjustable within the range from 5 to –5,and eyecups adjustable.These hold the eyepieces in the binocular tube.10 ErgoTube The viewing angle is adjustable within the range 10 – 50 .1 Magnification changerDrive knobs with magnification scale.MS5: 5 stepsMZ6: 6:1 zoomLeica Designby Ernest Igl / Christophe ApothélozMZ75: 7.9:1 zoomMZ95: 9.5:1 zoomMZ125: 12.5:1 zoomMZAPO: 10:1 zoom.MS52 Engaging ratchet positionsMZ6: Switch engages 7 magnification stepsMZ75: Milled ring engages 8 magnification steps8MZ95: Switch engages 9 magnification stepsMZ125: Switch engages 10 magnification stepsMZAPO: Switch engages 9 magnification steps.3a Focusing drive3b Coarse and fine focusing975Larger knob: Coarse focusingSmaller knob: Fine focusing.3c Adjustable ringChanges the ease of movement of the coarse/fine drive.4 Clamping screwHolds the optics carrier in the microscope carrier.5 Clamping screwHolds the binocular tube (or accessory) to the optics carrier.61463a

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Short instructionsInspect the cleanlinessChecklist왘 Clean: Optical accessories, glass stage plates, and anygraticules used (page 39).왘 Remove dust and dirt from the instrument (page 39).In this overviewOnly for variable ErgoModules we have summarized the working sequence. You will finddetailed user instructions on the pages indicated.왘 Adjust viewing height (page 9)Only for photomicrographic outfits왘 Lever at position “VIS”.왘 For trinocular tube: Push diaphragm lever to the right.왘 Open double-iris diaphragm (position “OPEN”).Adjust the interpupillary distance왘 Using both hands, displace the eyepiece tubes (page 9).Select the degree of eye contact왘 Secure the eyecup in the position required (page 9).Adjust the dioptric setting왘 Adjust the dioptric setting precisely for each eyepiece(page 12).MZAPOSet the magnification required8왘 Turn the rotary knob to the desired magnification (page 10).Focusing97왘 Look into the eyepieces and focus on the object (page 11). 10Only for coarse/fine drive:Focus approximately with the large knob, and thenaccurately with the small knob.52왘 If the focusing movement is too loose or too tight, adjust theease of movement (page 11).1463bAdjust the depth of field3c왘 Adjust the depth of field with the double-iris diaphragm(page 33).Microscope carrier AX왘 For measuring, photography and polarization, engage thevertical beam path (page 12).8

UseInterpupillary distance Ergonomics, viewing height, interpupillary distance,eye contactThe interpupillary distance is correct when you see a singlecircular field when viewing with both eyes.왘 Look into the eyepieces and useboth hands to move the eyepiece tubes closer together orfurther apart.ErgonomicsThe reasons why users often cannot work comfortably with the45 tube are the differing heights of the outfits, the variousaccessories and working distances, and the fact that the sameinstrument is often used by several people of differing builds.This problem can be effectively solved by using the LeicaErgoModules .If you have problems with the viewing configuration on yourstereomicroscope, ask your Leica consultant for the mostergonomic Leica solutions.Viewing height, viewing angle The viewing height is correct when your head and back arecomfortably positioned. Locating the eyepoint For 10 /21B wide-field eyepieces for spectacle wearers,the distance from eye to eyepiece is about 22mm. When you can see the entire field of view without restriction,you have found the correct position (eyepoint).왘 To find the eyepoint, slowly bringyour eyes closer towards theeyepieces.On the ErgoTube , the viewingangle is adjustable from 10 –50 .왘 Holding the eyepiece tubes attheir bases, swing them up ordown (see arrow).Selecting the degree of eye contactIf you do not wear spectacles, and want closer contact to theeyepieces:왘 Holding the diopter ring, turn theeyecup anticlockwise until it isreleased.ErgoModule ErgoWedge 5 – 25 ErgoWedge 15 ErgoModule 50mm The ErgoTube 45 has eyepiece tubes which are 65mmlonger than the standard ones. ErgoModule 30mm – 120mm 왘 Pull the eyecup upwards.왘 Holding the diopter ring, turn theeyecup clockwise.After several years, regreasingmay be necessary. Send theErgoModule to the Leicaservice workshop.Spectacle wearers need the wholeof the available distance. Movethe eyecups to the lowest position.9

UseScale of magnificationsMagnification changerMS5, MZ6MS5: 5 stepsMZ6: 6:1 zoomMZ75: 7.9:1 zoomMZ95: 9.5:1 zoomMZ125: 12.5:1 zoomMZAPO: 10:1 zoomDisplay of magnification factors from 0.63 – 4. MZ75Display of magnification factors from 0.63 – 5. For other objectiveeyepiece combinations, appropriate adhesive scales can beapplied to the rotary knobs (page 19).Zoom means continuous change of magnification. Ratchetscan be engaged for certain magnifications (see below).MZ95Display of magnification factors from 0.63 – 6.Changing the magnification The magnification changer can be operated from either theright or the left.MZ125Display of magnification factors from 0.8 – 10.왘 Look into the eyepieces.왘 Focus on the object (page 11).MZAPO왘 Turn the magnification changerto the position required.Display of magnifications with various optical combinations,e.g. with 1.0 objective and 10 eyepieces: 8 – 80 .MZAPO: Setting the scaleThe magnification scale can be set in accordance with theoptical combination.Ratchet stepsMZ6: 0.8, 1.0, 1.25, 1.6, 2, 2.5, 3.2MZ75: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4MZ95: 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5MZ125: 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3, 8MZAPO: 10, 12.5, 16, 20, 25, 32, 40, 50, 63 Engage the ratchets whenever magnifications need to beexactly reproducible.왘 For MZ6, MZ95, MZ125 andMZAPO: Press the switch.Example:1.0 objective / 25 eyepiece왘 Turn the magnification changerto the stop at its lowest magnification position.왘 Read the magnification rangefrom the table on page 43 (in theexample given, 20 to 200 ).왘 Place a finger in the rearwindow of the magnificationchanger and displace thescale so that the position 20is lined up with the index.Magnifications and fields of view왘 For MZ75: Turn the knurled ringanticlockwise.10The tables between page 41 and page 43 provide informationabout the magnifications and field diameters as a functionof the magnification changer position of the eyepiece-objectivecombination used.

Magnification with MZ125/MZAPO Pan and Planapo objectivesAdjusting the free running of the focusing driveWhen the MZ125/MZAPO planachromatic and planapochromaticobjectives are used on the Leica MS5, MZ6, MZ75 and MZ95,the magnification is increased by the factor 1.25 . This factoris taken into account in the tables.Does the focusing drive run too loosely or too tightly, or doesthe equipment drift downwards?You can match the ease of movement to the weight of the outfitand to your personal requirements.Magnification onMZ125/MZAPOMagnification onMS5/MZ6/MZ75/MZ951.0 Plan/Planapo1.25 1.6 Planapo2.0 0.63 Planapo0.8 0.5 Plan0.630.8 Plan1.0 Setting the working distance, focusing obtaining a sharp picture To focus, use the focusing drive to raise or lower thestereomicroscope until the feature of interest is in focus.The working distances of the various objectives are listed inthe tables on pages 41–43. The focusing drive can be operated from either the right orthe left.Focusing drive왘 Grip the drive knobs firmly withboth hands and turn them inopposite directions until youobtain the required degree ofresistance during focusingFocusing drive, coarse / fine왘 Turn the black ring on theright-hand drive knob until youobtain the required degree ofresistance during focusing.왘 Place the object beneath theobjective.왘 Engage the lowest magnification(page 10).왘 Look into the eyepieces.왘 Using the rotary knob, bring theobject into focus.Only for coarse / fine drives왘 Focus approximately with thelarger knob.왘 Focus accurately with thesmaller knob.11

UseMicroscope carrier AXMicroscope carrier: Tu

manual in detail. If you still have a question sometime, just contact your local Leica agency or get in touch directly with Leica Microscopy Systems Ltd, Heerbrugg, Switzerland. We are gladly at your service. Customer service is a big thing with us. Not only before the sale, but afterwards as well.* * The business units of Leica Microsystems hold

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