UHPLC / HPLC Columns Developed For Mass SpectroMetry
ACE UltraCore SuperC18 UHPLC / HPLC Columns Developed forMass Spectrometry Water analysis Pharmaceutical Environmental Toxicology Food safety Clinical Forensics Metabolism Trace analysis Ultra-inert 2.5µm and 5µm solid-core particles for sharp peaks and high MS signal sensitivityUltra-low bleed profile for minimal background in all UV and MS applicationsColumns stable between pH 1.5 and pH 11 for maximum versatilityRapid analysis formats available including 0.5mm and 1.0mm id columnsSolid-Core UHPLC / HPLC Columns for MS
ACE UltraCore SuperC18Explore the advantages of ACE UltraCore SuperC18 for MSACE UltraCore SuperC18 has been specifically designed for MS applications and is based onthe high efficiency, low back pressure solid-core particles with our unique Encapsulated BondingTechnology (EBT ). This technology dramatically increases ligand coverage on the solid-coresilica surface and consequently provides a number of key advantages for MS users.Advantage #1: Reduced Background Noise for MSThe proprietary Encapsulated Bonding Technology (EBT ) ensures ACE UltraCore SuperC18columns are highly resistant to hydrolysis. The resulting highly stable phase contributes minimalbackground column bleed ensuring maximum MS response and intensity.ACE UltraCore SuperC18 Columns Provide Exceptionally Low MS Bleed The following example compares bleed from a gradient analysis as a Total Ion Chromatogramdetected by the MS with and without the presence of a highly stable ACE UltraCore SuperC18 column.System Background NoiseApplication # 1803Total Ion Chromatogram (scanning 50-1000 amu)(generated using 1m empty 00500,0000102345768910Time - MinutesACE UltraCore SuperC18Total Ion Chromatogram (scanning 50-1000 amu)2,500,000 ACE UltraCore SuperC18 showsexceptionally low bleed and effectivelyprovides the same low level ofbackground noise as the “system only”configuration – perfect for 678910Time - MinutesColumn: ACE UltraCore SuperC18, 50 x 2.1mm, 5µmFlow Rate: 0.60ml/min Temp: 40 C Detection: Agilent 1290B with 6150MSD, AJS-ES spray chamberMobile Phase A: 0.1% v/v HCOOH (aq)Mobile Phase B: 0.1% v/v HCOOH in MeCNGradient: Time (mins)00.261010.5%B55100 10052www.ace-hplc.comACE UltraCore SuperC18 UHPLC / HPLC Columns for MS
Advantage #2: Improved Peak Shape and MS Signal IntensityACE UltraCore columns have earned a well deserved reputation for delivering highly efficient,symmetrical peak shapes even with the most challenging of molecules as illustrated by thefollowing independent test. The use of a highly efficient column exhibiting minimal peaktailing will result in an improved MS signal response.ACE UltraCore SuperC18 Provides Exceptional Efficiency Leading column brands from major manufacturers investigated Comparison of column efficiency for pyridine – a basic moleculePeak Efficiency ComparisonReproduced with kind permission of The Open University, UK.EfficiencyPressure117barACE UltraCore 2.5 SuperC18450 barPhenomenex Kinetex 1.7 C18235barWaters Cortecs 1.6 C18285bar35,200pl/m157 bar34,800pl/m117 barThermo Accucore 2.6 C180Higher Efficiency49,100pl/m49,100pl/mPhenomenex Kinetex 1.3 C18Phenomenex Kinetex 2.6 C18Application # 180244,400pl/m35,600pl/m19,200pl/m20,00040,000N0.5 (pyridine) (plates/m)60,00080,000Lower EfficiencyColumn Dimensions: 50 x 2.1mm Sample: 1) uracil 2) pyridine 3) phenolMobile Phase: 30:70 (v/v) MeOH/10mM NH4OAc in H2O (pH 5.8) Flow Rate: 0.20ml/min Temperature: 22 C Wavelength: 254nmComparative data may not be representative of all applications. Please see back page for acknowledgement of trademarks.Reduce Peak Tailing to Improve MS Signal Response Improved efficiency and peak shape has a direct effect upon signal responseIncreasedResponseIncreasedTailing ReducedPeak HeightReducedTailing IncreasedPeak Height Improve your MS signalresponse by selectinghigh efficiency, low tailingACE UltraCore SuperC18columns3www.ace-hplc.comACE UltraCore SuperC18 UHPLC / HPLC Columns for MS
Advantage #3: Exploit a Wider pH Operating RangeA further advantage of the proprietary Encapsulated Bonding Technology (EBT ) isthe extended pH operating range compared to traditional C18 phases.Highly stable ACE UltraCore SuperC18 columns can be used with MS compatible buffersfrom pH 1.5 – 11.0 to maximise both resolution and MS signal response.Exploit Selectivity by Adjusting pH with MS Compatible Buffers Confidently develop methods at an eluent pH that maximises resolution and MS response Stable and rugged ACE UltraCore columns offer excellent column lifetimes across the pH rangeApplication # 1801pH 3.0pH 18, 9(20mM Ammonium formate)pH 232pH 31012114pH 46157pH 5012345pH 10.7Method Development Tip:34(0.1% NH3)92pH 6 8612At elevated pH, the use of a 0.1% NH3LC/MS compatible mobile phase isparticularly recommended due to easeof preparation and to maximisecolumn lifetime.10pH 7pH 8111pH 957pH 10012Time - Minutes345pH 11Column: ACE UltraCore SuperC18, 50 x 2.1mm, 2.5µmSample: 1) atenolol 2) methylphenylsulphoxide 3) eserine 4) prilocaine 5) bupivacaine 6) tetracaine7) 1,2,3,4-tetrahydro-1-naphthol 8) carvedilol 9) nitrobenzene 10) methdilazine 11) amitriptyline 12) valerophenoneTemperature: 40 C Flow Rate: 0.60ml/min Wavelength: 254nm Gradient: 3 – 100% B in 5 minutesAcidic Mobile Phase: A: 20mM ammonium formate in H2O (pH 3.0) B: 20mM ammonium formate (pH 3.0) in 90:10 (v/v) MeCN/H2OBasic Mobile Phase: A: 0.1% v/ v NH3 ( 18mM) in H2O (pH 10.7) B: 0.1% v/ v NH3 ( 18mM), pH 10.7 in 90:10 (v/v) MeCN/H2O4www.ace-hplc.comACE UltraCore SuperC18 UHPLC / HPLC Columns for MS
Use ACE UltraCore SuperC18 for MS ApplicationsExample #1 Low Level Determination of Multiple Pesticide Residues by LC/MS/MSApplication # 1804Reproduced with kind permission of Kent Scientific Services, UKCountsCarbarylAdditional pesticides withinthe same AldicarbAldicarb sulphoneAldicarb ClothianidinCyfluthrinDemeton S-methylsulphoneDemeton mThiodicarbThiophanate methylTriforineFenbuconazoleCountsTransition337.10 70.00Visit www.ace-hplc.comfor further MS application detailsTime - MinutesColumn: ACE UltraCore SuperC18, 50 x 2.1mm, 2.5µm Temp: 40 C Flow Rate: 0.40ml/min Detection: Agilent 6420 triple quadrupole MS, ESI ve mode, dynamic MRMMobile Phase A: 0.1% v/v HCOOH 5mM ammonium formate in 10:90 v/v MeOH/H2O Mobile Phase B: 0.1% v/v HCOOH 5mM ammonium formate in 90:10 v/v MeOH/H2OGradient: Time (mins) 01 1518 18.05 20%B 00 100 10000Example #2 Multiple Veterinary Steroids and Various Epimers by LC/MS/MS(with Positive/Negative Switching)Application # 1805Reproduced with kind permission of The Food and Environment Research Agency, UK-ESI .007.008.008.009.0010.009.00Test 00210011.005: MRM of 1 Channel ESDiethylstilbestrol-d8Transition275.23%Taleranol and zeranol-d4Taleranol and estrol11.0010.00transAdditional –ve mode(-ESI) analytes:α- and β-Zearalenol275.12245.09cis02.503.003.004.00 .00 7.00Test 002 100Test 0045.005.50β2.503.003.50Test 0044.004.505.00Time - Minutes6.006.505.506.00121.127.00 of 1 Channel7.504: MRMES 5: MRM of 1 Channel ES α- and β-Nortestosteroneα%0Additional ve mode( ESI) analytes:α- and β-BoldenoneβTest 0047.5011.003: MRM of 1 Channel bolone7.503: MRM of 1 Channel ES Column: ACE UltraCore SuperC18, 100 x 2.1mm, 2.5µm Flow Rate: 0.50ml/min Temp: 45 C Detection: Waters Xevo TQS MS, ve or –ve mode as required, MRM data Mobile Phase A: 0.01mM NH4F 0.001% v/v HCOOH (aq) Mobile Phase B: MeCNGradient: Time (mins)0 0.5 77.5 10.5%B25 25 35 35 60 Visit www.ace-hplc.comfor further MS application details5www.ace-hplc.comACE UltraCore SuperC18 UHPLC / HPLC Columns for MS
Product Availability and SpecificationsFunctional GroupParticleSize (µm)Pore Size(Å)SurfaceArea (m2/g)CarbonLoad (%)MaximumpH RangeUSPListingACE UltraCore 2.5 SuperC18Octadecyl encapsulated2.5951307.01.5-11.0aL1ACE UltraCore 5 SuperC18Octadecyl encapsulated5951005.41.5-11.0L1Phasea CE UltraCore columns are designed for use with LC/MS compatible buffers. Further information is contained within “ACE UltraCore – A Guide to Buffer Selection” Aplease contact your distributor to request your FREE copy or visit www.ace-hplc.com.aImportant Note: ACE UltraCore microbore columns (1.0mm id and 0.5mm id) are available with either standard 1/16” (10-32 thread) connections or 1/32” (6-40 thread)connections. For use with Eksigent micro and nano LC systems, order columns with 1/32” connections and use either ACE 6-40 fittings (part number ACE-MC3210,10 pack) or Eksigent 6-40 fittings (part number 5019621).bACE UltraCore 2.5µm SuperC18 UHPLC/HPLC Columns (UHPLC/HPLC hardware format with 1000 bar/15000 psi pressure limit)Column -1546UACE UltraCore 2.5µm SuperC18 Microbore HPLC Columns (HPLC hardware format with 400 bar/6000 psi recommended pressure limit)Column mm (1/32” ORE-25A-10005CORE-25A-12005CORE-25A-150051.0mm (1/32” 01CORE-25A-1201CORE-25A-1501bACE UltraCore 5µm SuperC18 UHPLC/HPLC Columns (UHPLC/HPLC hardware format with 1000 bar/15000 psi pressure limit)Column CORE-5A-1546UCORE-5A-2546UACE UltraCore 5µm SuperC18 Microbore HPLC Columns (HPLC hardware format with 400 bar/6000 psi recommended pressure limit)Column mm0.5mm (1/32” -250051.0mm (1/32” A-1001CORE-5A-1201CORE-5A-1501CORE-5A-2501For 1/16” HPLC column connections up to 6000 psi, PEEK 1/16” fingertight fittings (part number ACE-CC10, 10 pack) are recommended. For 1/32” Microbore HPLC columnconnections up to 6000 psi, PEEK 1/32” (6-40 thread) fingertight fittings (part number ACE-MC3210, 10 pack) are recommended. For 1/16” UHPLC column connections upto 25000 psi, reuseable 1/16” fittings (part number EXL-CC10, 10 pack) are recommended. To further extend UHPLC and HPLC column lifetimes, ACE pre-column filters arerecommended. For further details please contact your distributor or visit www.ace-hplc.comACE is a registered trademark of Advanced Chromatography Technologies Limited. ACE UltraCore , SuperC18 and EBT are trademarks of Advanced ChromatographyTechnologies Limited. Advanced Chromatography Technologies Limited acknowledges the registered and unregistered trademarks of Eksigent Technologies, Phenomenex Inc.,Thermo Fisher Scientific, Victrex Plc and Waters Corporation and has no affiliation with any of these companies.ACE UltraCore columns are available through our international distributor network:Solid-Core UHPLC / HPLC Columns for MSwww.ace-hplc.comAdvanced Chromatography Technologies Limited, 1 Berry Street, Aberdeen, AB25 1HF, ScotlandTel: 44 (0) 1224 704 554 Fax: 44 (0) 1224 841 301 email: info@ace-hplc.com www.ace-hplc.com12000Q/1214/003-A1525V1
(generated using 1m empty capillary) Time - Minutes Time - Minutes 2 www.ace-hplc.com ACE UltraCore SuperC18 UHPLC / HPLC Columns for MS Counts Counts 500,000 1,000,000 1,500,000 2,000,000 . www.ace-hplc.com ACE UltraCore SuperC18 UHPLC / HPLC Columns for MS Transition 202.10 145.10 Transi
Jun 01, 2016 · THIAMINE HYDROCHLORIDE HPLC H ilic HPLC HPLC to Hilic HPLC Add HPLC ID test Remove pH test THIAMINE MONONITRATE HPLC Hilic HPLC HPLC to Hilic HPLC Add HPLC ID test Remove pH test NIACINAMIDE n/a n/a Remove Melting Range ADENINE HPLC to UPLC HPLC to UPLC n/a CALCIFEDIOL n/a add HPL
zero-dead volume fittings is generally preferred for UHPLC experiments. The injection volume should be selected in agreement with col-umn geometry. A rule of thumb is to maintain the injected vol-ume between 1 and 5% of the column dead volume. As most of the experiments carried out in UHPLC are performed with a 50x2.1 mm column (V 0
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Title: New HPLC Column Options for UHPLC Author: Maureen Joseph Created Date: 3/18/2013 2:48:10 PM
The cross-particle scalability of Kinetex EVO C18 (from 5µm to 2.6 µm to 1.7 µm) particles sets it apart from other core-shell products outside the Kinetex product family. Now you can smoothly scale core-shell pH 1-12 methods from UHPLC to HPLC to Preparative HPLC. Imagine how much development time this will help save
BUILD BETTER HPLC METHODS WITH CRAWFORD SCIENTIFIC A collection of articles designed to help improve your HPLC Method Development knowledge and skills. VOLUME III . HPLC Method Development Volume III Page 2 TABLE OF CONTENTS P.03 P.08 P.17 P.25 UNDERSTANDING STATIONARY PHASES FOR HILIC SEPARATIONS HAVE WE FORGOTTEN THE ADVANTAGES OF CORE-SHELL PARTICLES? PRACTICAL HPLC METHOD DEVELOPMENT .
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AGMA American Gear Manufacturers Association AIA American Institute of Architects. AISI American Iron and Steel Institute ANSI American National Standards Institute, Inc. AREA American Railway Engineering Association ASCE American Society of Civil Engineers ASME American Society of Mechanical Engineers ASTM American Society for Testing and .
