THIS IS HTS WITH A WHOLE NEW ATTITUDE - PerkinElmer
THIS IS HTS WITHA WHOLE NEWATTITUDEEnVision Multimode Plate ReaderFor research use only. Not for use in diagnostic procedures.
TAKE A FRESH LOOKAND DISCOVERALL THAT'S NEWAlready the platform of choice for HTS, the tried and tested EnVision plate reader is now even better,with an array of exciting enhancements that can take your applications to the next level. The systemprovides even higher sensitivity for time-resolved fluorescence (TRF) applications. It continues todeliver optimal results from our proprietary Alpha, LANCE , and DELFIA assay technologies. And ourupdated software provides tools to facilitate 21 CFR Part 11 compliance for integration into regulatedenvironments (GxP).This is globally established, robust, proven technology, with more than 14,000 (and counting) peerreviewed, published papers to its credit. And with a robust network of service engineers and applicationscientists across the globe, you can count on us to support you and your applications.The EnVision system: Tried, tested, and proven to inspire science with attitude.EnVision Multimode Plate Reader2018 Winner of SelectScience Seal of Quality AwardSelectScience Seals of Quality recognize the top 0.1% oflaboratory products that consistently receive the highestcustomer review ratings and are designed to further assistscientists worldwide in making important purchasingdecisions for their labs.2
Better speed, throughput, and sensitivityThe EnVision multimode plate reader provides exceptionalspeed, ultrahigh throughput, and maximum sensitivity acrossall detection technologies. The gold standard for highthroughput screening, the EnVision microplate reader givesyou robust performance and reliable data, time after time,with exceptional sensitivity and minimal downtime.A broad selection of proven technologiesThe standard EnVision system configuration reads alltraditional detection technologies: Absorbance Fluorescence intensity Luminescence, including glow, flash, and dual luminescence Time-resolved fluorescence (TRF) and TR-FRET, includingour DELFIA, LANCE, and LANCE Ultratechnologies, LanthaScreen , andcertified for use with HTRF technology Fluorescence polarizationWhat's more, the EnVision platform supports all thesedetection technologies with kinetic and scanningmeasurements, a wide range of filters, dichroic mirrors,and bottom reading for fluorescence intensity and TRFmeasurements. And it accepts all standard microplates,including 96-, 384-, 1536-, and 3456-well formats.Increase your capabilities, capacity, and throughputYou can also extend your lab’s assay capabilities with advancedoptions such as: Laser technology for our proprietary AlphaScreen and AlphaLISA technologies, including AlphaPlex and Alpha CETSA Ultrasensitive luminescence TRF laser module Quad-monochromatorsWe offer a wide choice of label-specific bar-coded filters andoptical mirror modules for almost all assays – and if youroptimal optics aren’t in our standard portfolio, we can providecustomized modules for you. Plus, you can add a dispenser forfast response cell-based assays and plate stackers to increasecapacity and throughput.www.perkinelmer.com/EnVisionThe Solution of Choicefor All Your AssaysCellularBottom-reading, shaking, scanning, temperaturecontrol, and kinetics capabilities make EnVisionthe go-to reader for cellular assays.GPCRHigh-throughput read technologies are perfectfor a wide range of GPCR assay types, such asAlphaScreen, LANCE, and AequoScreen .Reporter GeneScanning and kinetics capabilities and labelspecific optical mirror modules and filters enableGFP assays and dual reporter-gene assays usingluciferase and beta lactamase.EnzymeAccurate, stable temperature-control andrestacking capabilities let you perform sensitivekinetic measurements across a wide dynamicrange and high-speed measurements at shortrepeat intervals.KinaseLANCE Ultra / TR-FRET biochemical assaysare ideal for tyrosine/serine/ threonine kinases.Alpha technologies measure phosphorylatedpeptides, full protein substrates, and endogenousphosphorylated proteins in a cellular format.QuantificationA range of filters and optical mirror modulescovering the UV/Vis spectrum enable directmeasurement of DNA, protein, and classicalELISAs; AlphaLISA, AlphaScreen, andDELFIA capabilities offer wider dynamic rangeand higher sensitivity.3
WHAT DOES YOURIDEAL PLATE READERLOOK LIKE?The EnVision multimode plate reader comes in twoconfigurations: You can choose the EnVision 2105XCite reader for the ultimate in sensitivity – anaffordable single-detector system ideal for the mostchallenging research and assay development applications.Or opt for the EnVision 2105 HTS system – a superfast,dual-detector reader designed specifically to supportdemanding high-throughput discovery applications. Bothinstruments offer the same unsurpassed sensitivity andanalytical performance.ExcitationEnVision 2105 XCite light-pathEnVision 2105 HTS light-pathWorking together with the EnVision reader’s label-specific optical modules,the Proprietary Direct Optics system contributes to the instrument’sexceptional sensitivity.Innovative hybrid design for maximum flexibilityEnVision plate readers come with filter-basedtechnology for absorbance, fluorescence, luminescence,TRF, and fluorescence polarization. The systems canalso be equipped with quad monochromators forabsorbance and fluorescence, enabling you to chooseany wavelength and perform wavelength scans formaximum flexibility.Ultimate performance in time-resolved fluorescenceFlash lamp-based TRF is standard on the EnVision systemand offers good signal-to-noise ratios and speed foreveryday applications. For higher speed and sensitivity,you can choose a high-energy laser which delivers afaster decaying pulse that enhances long-lived dye signalbut decays completely by the time of measurement.New optical components employing advanced opticaltechnologies further improve the inherently excellentsignal-to-background ratio of the EnVision TRFtechnologies. And our proprietary LANCE, LANCE Ultra,and DELFIA assay technologies are developed andoptimized on the EnVision plate reader.4Emission 615 nmExcitationFRETULightTR-FRET 665 nmEuIn comparison, a Xenon lamp excitation pulsedecays slower, with increased background noiseduring measurement. The TRF laser therefore givessuperior signal-to-noise ratios and exceptionally fastmeasurement times – less than 36 seconds for a 1536well plate, including loading times.Lanthanides such as Europium decay slowly. In TRF, measurement is delayedafter excitation until background emissions have died down (blue: excitationpulse (laser); yellow: fluorescence signal from lanthanide; red: detectionperiod). In addition, emissions are redshifted, further reducing noise.Eu-labeled ULight-labeledProteinProteinLANCE Ultra assay principle: LANCE Ultra uses a Europium (Eu) chelatedonor dye, and a small, redshifted ULight acceptor dye. ULight dye providesa strong signal with minimal steric hindrance, which is perfect for kinase andGPCR applications. Ideal for high-throughput screening, LANCE Ultra TRFRET assays are sensitive, homogeneous, and easy to use.
Alpha Technology: The Assay That Measures It AllAlpha technology is a bead-based proximity assay. When Alpha Donor and Acceptor beads are brought together, a cascade of chemical reactions is set in motion,creating a greatly amplified signal. The highly versatile beads can be coated with various biomolecules, enabling detection of unique biological events. There’s anAlpha technology solution for just about every research application, and subsequent detection using the Alpha-proven EnVision multimode plate reader. What'smore, the Alpha Toolbox enables you to set up your own assay using ready-to-use Alpha beads for numerous capture solutions.AlphaLISA AlphaLISA SureFire Ultra AlphaScreen For results in half the time of ELISA, withoutwash steps, and with excellent sensitivity in avariety of matrices, including serum and plasmaDetects phosphorylated proteins faster, in morecomplex samples, and with better sensitivitywith the use of CaptSure technologyPerfect for studying fusion-tagged proteins orprotein:protein interactions, and for testingcAMP, cGMP, phosphorylated peptides, orsamples in a simple matrixAlphaPlex Alpha CETSA Enables detection of multiple analytes in asingle well for faster and more relevant results,even in low sample volumesEnables you to assess target engagementearlier in the drug discovery process, so youcan disqualify compounds earlier and havegreater confidence in promising onesTwo modes of detection to meet your needsTwo Alpha detection modules are available for the EnVision reader, each with a dedicated excitation laser. The standard Alphadetection module has a unique plate temperature-control mechanism that minimizes the effect of temperature gradients on assayperformance. This system also delivers all the speed necessary for utilizing AlphaPlex detection technology.The HTS Alpha detection module is more than twice as fast as the standard module, due to its special optical design.Ultrasensitive luminescence that makes the most of cells and reagentsWorking with primary, stem or difficult-to-transfect cells presents a unique set of challenges. Optional ultrasensitive luminescenceboosts sensitivity by almost 10 times versus standard luminescence by bringing the detector closer to the sample – giving you moreinformation from every cell. With the ultrasensitive luminescence option, the time per 384-well plate decreases from over one hourto less than two minutes – not only freeing up the instrument, but also minimizing drift effects.Alpha HTS module: Whilethe signal is measured in onewell, the beads in the nextwell are excited by the laser.The detector is placeddirectly above the plate forminimal signal loss.www.perkinelmer.com/EnVisionLuminescence measurementtime and sensitivitycomparison: StandardEnVision system versus anEnVision reader withultrasensitive luminescence,using a white ProxiPlate -384.5
APPLICATION OPTIONSAND SOFTWARE FOREVEN MORE FLEXIBILITYYou can complement the EnVision system’s alreadyunmatched speed with optional automation andaccessories that increase throughput and extend your lab’scapabilities to include time-course measurements, enzymeassays, and many other cell-based assays. For the highestthroughput applications, the EnVision plate reader canbe easily integrated into complete robotic solutions fromPerkinElmer and other automation providers.Temperature controlTo ensure reproducibility of cellular and enzyme kinetic assaysand other assays that require a defined temperature range, theEnVision platform’s temperature control option lets you preciselyregulate from 2 C to 55 C above ambient. Temperaturecontrol is uniform across the plate, and a top-heating plateeliminates condensation problems.DispenserThe EnVision dispenser can be used with 1- to 384-well platesin all reading modes. It consists of two pump units, which allowprecise delivery of reagents in 1 μl increments over a volumerange of 2 μl to 475 μl. In addition, a magnetic stirrer and aheater for the reagent bottle enable dispensing of cells, with anadjustable dispensing speed setting that lets you optimize forviscosity, cell density, and other assay requirements.Plate stackerAvailable for 20 or 50 plates, interchangeable plate stackermagazines can easily be added to increase capacity andthroughput. For kinetic assays, the grip-fed restacking functionlets you maintain the order of plates in the stack, increasing thereliability of data.Flexible automationEnVision plate reader with dispenser and stackerFor increased throughput or walkaway convenience, we offera range of automated liquid-handling systems such as theJANUS G3 Automated Workstation. EnVision plate readersare also compatible with a wide range of third-partyautomation systems.The JANUS G3 Automated Workstation is a flexible liquidhandling solution able to handle a wide range of plate types.The JANUS system can be integrated with the EnVision readeras part of your workflow for online assays or for preparing assayplates offline via the PlateStak microplate storage system.6
Software that enhances productivityPowerful software makes it easy to take advantage of allthe performance and versatility of the EnVision platform.An intuitive interface with preset user-specific protocolsmakes setting up the system fast and easy. For dataanalysis, features such as automatic curve fitting andflexible interpretation of standards controls and unknownsgive the EnVision system the power to handle a broadrange of assay types. The software also integrates smoothlyinto your lab's network, and into lab automation and datamanagement systems.Software to facilitate complianceFor regulated environments, our Enhanced Security modeprovides technological controls and features that support 21CFR Part 11 compliance with access levels, data security, anda comprehensive audit trail of user actions.Seamless integration with MyAssays Desktop EnhancedSecurity data analysis software is also possible, ensuringdata integrity. My Assays Desktop offers advanced analysisand reporting features.Advanced data visualization and analysisWith TIBCO Spotfire , you can make the most of yourhigh-quality plate reader data by harnessing advanceddata visualization and analysis capabilities. The TIBCOSpotfire Analyst for Multimode Detection (MMD)package is tailored for plate reader data analysis, enablingyou to visualize, correlate, compare, and analyze yourdata – and share it freely with your colleagues.Assays and reagents for virtually any applicationOur industry-leading reagents and assays include: ELISA-alternative Alpha technology LANCE and LANCE Ultra TR-FRET DELFIA TRF lites luminescence assays AequoScreen luminescence assaysAnd if you don’t find what you need, our specialist team candevelop custom assay solutions for you.Better microplates mean better resultsWe offer a wide range of microplates for fluorescence,luminescence, and absorbance assays, such as the highlyversatile OptiPlate , shallow well ProxiPlate , 1/2 AreaPlate for low volume assays, and AlphaPlates to minimizecrosstalk in Alphatechnology assays.Count on our supportYour application needs are as individual as you are. So we takea consultative approach to every engagement with you. Ourexpert-global service and support teams, comprising dedicatedlab- and field-based applications specialists, can work with you inpartnership to overcome the unique challenges your applicationbrings. And what's more, we can offer IQ/OQ testing forinstruments including the EnVision plate reader.Assay specific templates for plate reader data allow rapid familiarizationwith TIBCO Spotfire .www.perkinelmer.com/EnVision7
Summary of EnVision multimode plate reader models and optionsBASE UNITEnVision HTS plate reader5 filter-based technologies (dual detectors): Abs, FI, Lum, FP and TRFEnVision XCite plate reader5 filter-based technologies (single detector): Abs, FI, Lum, FP and TRFOPTIONAL DETECTION TECHNOLOGY MODULESUltrasensitive luminescence moduleStandard Alpha moduleIncludes Alpha laserStandard Alpha module with temperature controlIncludes Alpha laserHTS Alpha moduleIncludes ultrasensitive luminescence module and Alpha laserHTS Alpha module with temperature controlIncludes ultrasensitive luminescence module, temperature control and Alpha laserTRF laser moduleQuad monochromator (fluorescence/absorbance)OPTIONAL ACCESSORIESTemperature control moduleDispenser2 channelStackerStacker magazine – 20 platesStacker magazine – 50 platesPlate barcode readerFor EnVision XCite onlySOFTWARE OPTIONSEnhanced security software packageFor more information visit www.perkinelmer.com/EnVisionPerkinElmer, Inc.940 Winter StreetWaltham, MA 02451 USAP: (800) 762-4000 or( 1) 203-925-4602www.perkinelmer.comFor a complete listing of our global offices, visit www.perkinelmer.com/ContactUsCopyright 2017-2019, PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.14586A(39900)PKI
throughput applications, the EnVision plate reader can be easily integrated into complete robotic solutions from PerkinElmer and other automation providers. The JANUS G3 Automated Workstation is a flexible liquid-handling solution able to handle a wide range of plate types. The JANUS system can be integrated with the EnVision reader
VacuFlush HTS-VG series (with vacuum generators) SeaLand HTS-T series (with discharge pumps) SeaLand HTS-Basic series (with fittings only) Holding tank system Instruction manual EN HTS-VG series HTS-T series HTS-Basic series Caution! This manual must be read and understood before installation, adjustment, service or maintenance is
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Multiple Tank Outlets: Most HTS models are equipped with two discharge outlets. One is for connection to a deck-mounted discharge fitting and one is for connection to an overboard discharge pump. HTS Components: Your HTS is built with high-quality SeaLand sanitation system components. See page 6 for details. SeaLand Diptube Discharge Outlet
perconductivity (HTS) in the late 1980s and the emergence of practical HTS tapes in the 2000s brought about a com-pletely new instrument based on the unique physics of these new materials. Although typical HTS materials operate at about -200 C, th
5 per pair, depending on the HTS subheading, on imported non-EU footwear under Chapter 64 of the HTS . In addition, duties paid are calculated into the 8 percent VAT tax due on footwear, so this will also raise the VAT payment on imports. The ACT is calculated at the 4 digit HTS heading l
