HTRF Europium Cryptate Donor / Red Acceptor Readout Setup .
HTRF Europium cryptate donor / Red acceptor readoutSetup recommendations for Envision equipped in Flash lampEnvision reader must be equipped with a specific optical device (mirror and filters), whichenables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Theratio* of the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation ofDelta F (%) which represents the relative energy transfer rate for each sample.Envision reader must be appropriately configured for HTRF readout by setting up themeasurement conditions in the software according to the following indications:SetupTop MirrorTRF Lance D407/D630 advanced dual mirror (#666) orTRF LANCE/DELFIA D400/D630 dual mirror (#662)TRF advanced single mirror (#615) orTRF LANCE/DELFIA D400 single mirror (#412)Excitation filterUV2 (TRF) 320 (#111)Emission filterAPC 665 (#205)2nd emission filterCY5 620 (#118) or 615 (#203)Measurement height12 for 384sv – 7 for 96LV otherwise must bedetermined via OptimizationCycle2000µsDelay60µsNumber of flashes100Number of flashes for 2nd detector100Total time of windows400µsThis reader only allows high performance HTRF measurement when assays are run inWHITE plates.
HTRF Terbium cryptate donor / Green acceptor readoutSetup recommendations for Envision equipped in Flash lampEnvision reader must be equipped with a specific optical device (mirror and filters), whichenables the measurement of both 620 or 495 nm cryptate and 520nm acceptor emissions.The ratio* of the two fluorescence intensities 520/620 or 520/495 (acceptor/donor) enablesthe calculation of Delta F (%) which represents the relative energy transfer rate for eachsample.Envision reader must be appropriately configured for HTRF readout by setting up themeasurement conditions in the software according to the following indications:SetupTop MirrorTRF D407/D505 advanced dual mirror (#664) orTRF D400/D505 dual mirror (#657)TRF advanced single mirror (#615) orTRF LANCE/DELFIA D400 single mirror (#412)Excitation filterUV2 (TRF) 340 (#101)Emission filterTrf 520 (#275) or (#226)2nd emission filter495 (#276) for simultaneous readouts (dual mirror)Note: CY5 620 (#118) or 615 (#203) can be used with dual mirrorbut 2 successive readouts should be done (520nm then 620nmor 615nm)Measurement height12 for 384sv – 7 for 96LV otherwise must bedetermined via OptimizationCycle2000µsDelay60µsNumber of flashes100Number of flashes for 2nd detector100Total time of windows400µsThis reader only allows high performance HTRF measurement when assays are run inWHITE plates.
HTRF Terbium cryptate donor / Red acceptor readoutSetup recommendations for Envision equipped in Flash lampEnvision reader must be equipped with a specific optical device (mirror and filters), whichenables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Theratio* of the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation ofDelta F (%) which represents the relative energy transfer rate for each sample.Envision reader must be appropriately configured for HTRF readout by setting up themeasurement conditions in the software according to the following indications:SetupTop MirrorTRF Lance D407/D630 advanced dual mirror (#666) orTRF LANCE DELFIA D400/630 dual mirror (#662)TRF advanced single mirror (#615) orTRF LANCE/DELFIA D400 single mirror (#412)Excitation filterUV2 (TRF) 340 (Barcode #101)Emission filterAPC 665 (Barcode #205)2nd emission filterCY5 620 (Barcode #118 or #203)Measurement height12 for 384sv – 7 for 96LV otherwise must bedetermined via OptimizationCycle2000µsDelay60µsNumber of flashes100Number of flashes for 2nd detector100Total time of windows400µsThis reader only allows high performance HTRF measurement when assays are run inWHITE plates.
HTRF Europium cryptate donor / Red acceptor readoutSetup recommendations for Envision equipped in LaserEnvision reader must be equipped with a specific optical device (mirror and filters), whichenables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Theratio* of the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation ofDelta F (%) which represents the relative energy transfer rate for each sample.Envision reader must be appropriately configured for HTRF readout by setting up themeasurement conditions in the software according to the following indications:SetupTop MirrorTRF LASER D407/D630 advanced dual mirror (#667) orTRF LASER D400/D630 dual mirror (#446)TRF LASER advanced single mirror (#616) orTRF LANCE/DELFIA D400 single mirror (#445)Emission filterAPC 665 (#205)2nd emission filterCY5 620 (#118) or 615 (#203)Measurement height12 for 384sv – 7 for 96LV otherwise must bedetermined via OptimizationCycle16600µsDelay50µsNumber of flashes50 for white plate / 100 for black plateNumber of flashes for 2nd detector50 for white plate / 100 for black plateTotal time of windows400µs
HTRF Terbium cryptate donor / Green acceptor readoutSetup recommendations for Envision equipped in LaserEnvision reader must be equipped with a specific optical device (mirror and filters), whichenables the measurement of both 620 nm or 495nm cryptate and 520 nm acceptoremissions. The ratio* of the two fluorescence intensities 520/620 or 520 /495(acceptor/donor) enables the calculation of Delta F (%) which represents the relative energytransfer rate for each sample.Envision reader must be appropriately configured for HTRF readout by setting up themeasurement conditions in the software according to the following indications:SetupTop MirrorTRF LASER D407/D505 advanced dual mirror (#665) orTRF LASER D400/D505 dual mirror (#447)TRF LASER advanced single mirror (#616) orTRF LANCE/DELFIA D400 single mirror (#445)Emission filterTrf 520 (#275) or (#226)2nd emission filter495 (#276) for simultaneous readouts (dual mirror)Note: CY5 620 (#118) or 615 (#203) can be used with dual mirrorbut 2 successive readouts should be done (520nm then 620nmor 615nm)Measurement height12 for 384sv – 7 for 96LV otherwise must bedetermined via OptimizationCycle16600µsDelay50µsNumber of flashes20Number of flashes for 2nd detector20Total time of windows400µs
HTRF Terbium cryptate donor / Red acceptor readoutSetup recommendations for Envision equipped in LaserEnvision reader must be equipped with a specific optical device (mirror and filters), whichenables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Theratio* of the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation ofDelta F (%) which represents the relative energy transfer rate for each sample.Envision reader must be appropriately configured for HTRF readout by setting up themeasurement conditions in the software according to the following indications:SetupTop MirrorTRF LASER D407/D630 advanced dual mirror (#667) orTRF LASER D400/D630 dual mirror (#446)TRF LASER advanced single mirror (#616) orTRF LANCE/DELFIA D400 single mirror (#445)Emission filterAPC 665 (#205)2nd emission filterCY5 620 (#118) or 615 (#203)Measurement height12 for 384sv – 7 for 96LV otherwise must bedetermined via OptimizationCycle16600µsDelay50µsNumber of flashes20Number of flashes for 2nd detector20Total time of windows200µs
Envision reader must be equipped with a specific optical device (mirror and filters), which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. The ratio* of the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%) which repr
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the requirements for an effective national system for blood donor selection; policy recommendations are provided on p. 5. Part 2 provides guidance on specific criteria for blood donor selection in relation to general donor assessment, donor 1 The term "blood donors" includes s of whole blood, red cells, platelets, plasma and
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and specific security keys in order to access/enter donor data. D2 Donor - General Provides data dictionary for the files used by the donor menu options/routines. See Appendix G for a listing of the data elements in the BLOOD DONOR file (#65) and the BLOOD PRODUCT file (#66). D3 Donor - General Provides the ability to set up a site parameter.
WIRING DIAGRAMS SERVICE MANUAL NUMBER 33 Page 3A-8 90-863757--1 DECEMBER 8.1 liter (496 cid) Electrical Wiring Diagrams Typical Starting System Components 72930 B S I a c d e RED/PUR BLK BLK RED RED YEL/RED YEL/RED YEL/RED YEL/RED YEL/RED RED/PUR RED/PUR YEL/RED 7 g i b f h a-Ignition Switch b-20 Amp Fuse c-Starter Slave Solenoid d-Circuit .
The World Bank’s Role in Financial Management of Donor Funds / 1 Financial Management in Practice / 3 Extensive experience in managing donor assets / 3 Financial management of donor funds / 4 Investment management of donor funds / 6 Intermediation services in the capit
(Please type or use blue or black pen) Donor Information: BUSINESS/DONOR NAME – FOR PROGRAM: (As it should appear in catalog) DONOR CONTACT NAME: DONOR ADDRESS: PHONE CITY: STATE: ZIP: EMAIL (This is how we will send you your receipt.
Although there are different types of reports, in general, an academic report is a piece of informative writing, an act of communication and an account of an investigation (Reid, 2012). An academic report aims to sell a product, idea or points of view (Van Emden and Easteal, 1995). It should inform, explain and persuade (Williams, 1995) by using well- organised research. Sometimes it will .
