Maximize Yield, Purity, And Integrity

Genomic DNA purification kits—blood and saliva samplesRapid and efficient purification of genomic DNA from human blood and salivaThe emergence of pharmacogenomic centers of excellencehas resulted in increasing needs for purification of highquality genomic DNA from a minimal amount of blood.Since samples are collected and shipped worldwide, everysample may differ with regard to storage and shippingconditions. Choosing the right genomic DNA purificationkit is important based on your needs and expected endresults. We offer a wide range of kits designed to isolategenomic DNA from your blood or saliva samples at thepurity and scale you need.Whole blood—MagMAX DNA Multi-Sample Ultra 2.0 KitThe Applied Biosystems MagMAX DNAMulti-Sample Ultra 2.0 Kit was used toAisolate DNA from whole blood (Figure 4).140gDNA yields (μg)25Donor 1 Linear recovery performed on theThermo Scientific KingFisher Duo PrimePurification SystemgDNA yields (μg)120–– 50–400 µL samples processed withsmall-volume protocol in 96-well plate–– 400 µL samples were processed usinglarge-volume protocol in 24-well plateDonor 2y 0.0597xR² 0.99931100D120D2151050080y 0.0442xR² 0.9995160100200300400500Volume whole blood (μL)40200 45 minutes total time; 5 minutes hands-on Results–– Linear recovery of DNA across small andlarge volume inputs–– High-purity gDNA05001,0001,5002,0002,5005,000Volume whole blood (μL)BSpectrophotometric ratio–– No sample volume normalization—run50 µL and 400 µL sample at the sametime on one plateA260/A2803.0A260/A2302.52.01.51.00.5050 μL100 μL200 μL400 μL 1,000 μL 2,000 μLDonor 150 μL100 μL200 μL400 μL 1,000 μL 2,000 μLDonor 2Figure 4. Recovery of genomic DNA (gDNA) from whole blood. Whole blood wasobtained from two donors, and gDNA was isolated from 50 μL, 100 μL, 200 μL, 400 μL,1 mL, and 2 mL of the blood, using the MagMAX DNA Multi-Sample Ultra 2.0 Kit and theKingFisher Duo Prime Purification System. A NanoDrop 8000 spectrophotometer wasused to calculate the yield and purity of the DNA. (A) Recovery was linear across the rangeof volumes of blood used, including the smaller volumes shown in the expanded inset.(B) Assessment of DNA purity by A 260/A 280 and A 260/A 230.14

Which genomic DNA purification kit is right for your blood or serum samples?DNAzol BDReagentPureLink GenomicViral RNA/DNAMini KitPureLink Pro 96 ViralRNA/DNA KitMagMAX-96 DNAMulti-Sample KitProcess the largest Fast isolation of gDNA High-yield, high-purity Rapid and automatedamount of bloodfrom bloodgDNA in a plate format extraction of DNABlood input500 µL200 µL200 µL50 µL–1 mLYield10–20 µg3–10 µg3–10 µg1.5–66 µgIsolation methodOrganic extractionSilica spin columnSilica filter plateScalable, flexible formatwith magnetic beadsHigh tionsCloning, PCR,sequencingCloning, PCR,Cloning, PCR,Cloning, PCR,sequencing, genotyping sequencing, genotyping sequencing, genotypingPrep time10–30 min15 min35 min45 minQuantity100 mL50 or 250 preps4 x 96 preps100 or 1,000 preps15

Genomic DNA extraction—accelerating cancer discoveryLiquid biopsy workflowAmplify andExtract DNA/RNAligate adaptersCollect bloodSingle blood tubeCell-free total nucleicacid purificationSequenceOncomine cfDNAlibrary prep kits for lungand breast cancer Isolate total nucleic acid frombody fluids Invitrogen DNApolymerase Compatible with Ion Torrent Oncomine assays Applied Biosystems thermal cyclersTemplating,sequencingDevelop reportAnalyze data inIon Reporter Software to generatetest report Prepare templateon the Ion Chef System, sequence onan Ion GeneStudio S5 series instrumentStart your cancer research with these MagMAX extraction kits:cfDNACell-free total nucleic acidSummaryIsolated cfDNA in 60 minutesw/ mag bead kitIsolate cfTNA in 90 minutesw/ mag bead kitSequential isolation of DNA and RNAfrom the FFPE tissue sample. DNA andRNA recovered in separate eluates.Sample volumes100 µL–10 mL2–4 mL; lower or higher if needed5–40 µm standard input of slidesor curlsElution volumes15–50 µL15–20 µL20–50 µLTime per run45–60 min90 min for 24 samples20 min for DNA, 48 min for RNAAutomation/HTAutomated or manualAutomated or manualAutomated or manualPurificationFree-circulating DNA (cfDNA)Free-circulating DNA, RNA, and miRNADNA and RNA from FFPEtissue samplesFormat50 samples for 2 mL plasma input25 samples for 4 mL plasma input50 samples for 2 mL plasma input25 samples for 4 mL plasma input48 samples for sequential DNA andRNA; 96 samples for DNA or RNA onlyFor more ion16Formalin-fixed, paraffin-embedded(FFPE) samples

Genotyping—pharmacogenomics studiesPharmacogenomics (PGx) is an important research areafor the development of psychotropic, cancer, pain, andcardiovascular medications. We offer a variety of solutionsto assist you with your PGx research.For scientists seeking preemptive PGx insight, theApplied Biosystems PharmacoScan Solution is an arraybased tool to assess 4,627 ADME markers within 1,191genes known to play roles in drug processing.For fewer than 120 targets, quantitative PCR usingwell-established Applied Biosystems TaqMan Assayscombined with the Applied Biosystems ProFlex PCRSystem or QuantStudio 12K Flex Real-Time PCRSystem and the Applied Biosystems OpenArray platform is designed to deliver a flexible, cost-saving, andhigh-throughput solution.Flexible, scalable, high-throughput, low-costKingFisherpurification systemsQuantStudio 12KFlex systemOpenArray technologyTaqMan DME AssaysTaqMan CNV AssaysAllele 2Allelic discrimination plotProFlex PCR System with dual flat block supports OpenArray plate technology.17

Plant genomic DNA isolation kitsSuccessful plant DNA extraction that’s easy on youPlant tissue is difficult to work with due to the highlevels of polysaccharides and polyphenols present.Compounding this complexity with tedious, inefficientmethods only prolongs the process of isolation. Cetyltrimethyl ammonium bromide (CTAB) methods requireexcessive time and handling, limiting your throughput.Some silica membrane and magnetic bead–basedprotocols don’t remove inhibitors inherent in plant samplesthat can carry over into the final product and interfere withdownstream applications.18This leads to frequent sample processing failure, requiringyou to repeat the purification.Our innovative products are specifically designed for easy,high-yield, and high-purity DNA purification from plantsamples. Inhibitors are removed for reliable downstreamresults. Make your DNA isolation from plant sampleseasier on you and on your samples, while achieving highyield, high-purity results with Applied Biosystems andInvitrogen plant molecular biology reagents.

Which genomic DNA purification kit is right for your plant samples?Plant DNAzolReagentPureLink GenomicDNA Plant Mini KitPureLink Pro 96Genomic DNA KitMost cost-effectiveHigh-quality gDNAat a great valueFast isolation of gDNARapid and automatedfrom a variety ofextraction of DNAplant samples100 mgUp to 25 mg100 mgPlant tissue input 100 mgMagMAX PlantDNA KitYieldVaries based onstarting material1–15 µg5–10 µg from tissueVaries based onstarting materialIsolation methodOrganic extractionSilica spin columnSilica filter plateScalable, flexible formatwith magnetic beadsNoYesYesHigh ning, qPCR,sequencingCloning, PCR,Cloning, PCR,Cloning, PCR,sequencing, genotyping sequencing, genotyping sequencing, genotypingPrep time60 min40 min35 min40 minQuantity100 mL50 preps4 x 96 preps96 or 384 prepsEmpower your plant science research: go tothermofisher.com/agbio to learn more and topurchase our products and solutions.19

Viral genomic DNA purification kitsHigh-yield genomic DNA from tissue and cellsPurification of viral nucleic acids poses unique challenges for getting goodrecovery and detection sensitivity. Capture and lysis of virus particles fromvery dilute solutions or cell-free samples is the first hurdle. We’ve developedDNA purification products that are optimized to provide maximum viral DNAyield, purity, and integrity from a broad range of sample types in severalformat options.Which DNA purification kit is right for your viral samples?PureLink ViralRNA/DNA Mini KitPureLink Pro 96Viral RNA/DNA KitMagMAX Viral/PathogenNucleic Acid Isolation KitFast isolation ofviral nucleic acidEasy to use withhigh sensitivityRapid and automatedextraction of viralnucleic acidSample input500 µL cell-free sample200 µL cell-free sample200–400 µL 96 deep-well plate,500–2,000 µL 24 deep-wellplate sampleCompatible samplesPlasma, serum,cerebrospinal fluidPlasma, serum,cerebrospinal fluidSalivaIsolation methodSilica spin columnFilter plateScalable, flexible format withmagnetic beadsHigh throughput–compatibleNoYesYesCompatible applicationsCloning, qPCR,sequencing, genotypingCloning, qPCR,sequencing, genotypingqPCR and sequencingPrep time15 min35 min40 minQuantity50 preps4 x 96 preps100 prepsGet more information about our viral RNA/DNA purification anddetection products at thermofisher.com/viral20

Genomic DNA from salivaThe Applied Biosystems MagMAX Saliva gDNA Isolation Kit is the fastest,most user-friendly saliva purification kit on the market. With minimal steps, anaffordable price, and comprehensive support, more saliva samples can beprocessed with consistent results.How it works for youFastAdaptableCompatibleAffordableAutomation ready20 min instrumenttime and only 5 min forsample processingRun 96 (200–500 μL) or24 (0.5–2 mL) samplesbased on your inputHigh-quality purifiedgDNA compatible withmicroarray, PCR, and NGSdownstream applicationsCompetitive low priceper reactionDesigned forThermo Scientific KingFisher instrumentsSimple workflow1Add 96 saliva samples andbinding beads/lysis reagentmixture to a 96-well plate.2Add reagents toworking plates.3Load plates on theKingFisher instrument.Fast processing time5 minMagMAX SalivagDNA Isolation KitConsistent gDNA yield Yield average 3.5 µg of gDNA20 min(Your time) (KingFisher instrument time)5 min(Your time)4Run instrumentfor 20 minutes. Total gDNA yield range 0.15–13.54 µg45 min(Instrument time)Supplier P kit 97% of donor saliva samples yieldedgreater than 300 ng of gDNA30 min*(Your time)Supplier N kit00.51Time (hr)21

Complete purification system for nucleic acids,proteins, and cellsAutomated sample preparationSuccessful downstream analysis depends on high-quality,reproducible purification of nucleic acids, proteins, andcells. KingFisher purification systems are designed todeliver high-quality results with minimal hands-on time,automating a significant part of your workflow. Choose from three distinct systems to meet yourapplication and throughput needs Optimized kits streamline the purification workflow for awide variety of sample types Thermo Scientific BindIt Software enables you tocreate customized protocols for additional flexibility Specially designed consumables allow efficientsample processing22Magnetic separation technologyKingFisher systems use permanent magnetic rodsand disposable tip combs to collect, transfer, and mixmagnetic particles:1. When the magnetic rod—sheathed inside the tip comb—is lowered into the solution, magnetic beads collect at thebottom of the tip comb.2. The tip comb is then positioned in a different row or plate,and the beads are released by moving the magnetic rodsout of the tip comb.3. The tip comb facilitates the mixing of reagents with thebeads as the magnetic head moves up and down.

Thermo Scientific automated sample preparation technologies comparison chartKingFisher Duo PrimePurification SystemKingFisher FlexPurification SystemKingFisher PrestoPurification SystemBenefitsAn economical option for automatednucleic acid purification and proteinpurification from up to 12 samples ata time and 24 samples per load usingmagnetic beadsA highly versatile and reproduciblepurification of 24 or 96 samples per runUtilizes magnetic particle–basedtechnology to provide high-qualityyields of target nucleic acids andproteins; compatible with a liquidhandler for plate filling in highthroughput laboratoriesApplicationsDNA and RNA isolation from variousstarting materials; proteomic applications;cell isolationDNA and RNA isolation from variousstarting materials; proteomic applications;cell isolationNucleic acid purification, proteinpurification, immunoprecipitation,antibody purification, phosphopeptideenrichment, phage displayReagentsPreloaded and user-editable MagMAX kits available to support tissue, cell, blood, FFPE, bacterial, buccal, plant, andviral samples, as well as liquid (e.g., serum)ProtocolUses BindIt Software or USBmemory deviceUses BindIt SoftwareUses BindIt SoftwarePlasticconsumables96 deep-well plate24 deep-well plate1 x 12 elution strip96 deep-well plate24 deep-well plate96-well plate96 deep-well plate24 deep-well plate96-well plateSample inputvolume30–1,000 μL (12-pin magnet head)200–5,000 μL (6-pin magnet head)50–1,000 μL, 96 deep-well plate200–5,000 μL, 24 deep-well plate20–200 μL, 96-well plate50–5,000 μL24- or 96-head magnetsThroughputUp to 12 samples with 12-pin magnet head 1 to 24 samples (24-well plate)Up to 6 samples with 6-pin magnet head1 to 96 samples (96-well plate)1 to 24 samples (24-well plate)1 to 96 samples (96-well plate)Instrumentdimensions(W x D x H)40 x 46 x 34 cm(15.7 x 18.1 x 13.4 in.)68 x 60 x 38 cm(26.8 x 23.6 x 15 in.)36 x 46.5 x 40 cm(14.2 x 18.3 x 15.5 in.)Weight17 kg (37.5 lb)28 kg (62 lb)24 kg (53 lb)Learn more at thermofisher.com/kingfisher23

Plasmid DNA purification kitsSuccessfully isolate high-quality plasmid DNAChoose from our wide range of high-performing, costeffective plasmid purification technologies designed to helpyou overcome common plasmid prep issues, such as lowrecovery and the presence of impurities.Invitrogen PureLink plasmid purification products weredeveloped to provide the greatest value for your resources.With fair pricing and performance meeting or exceedingofferings from other suppliers, PureLink technology isthe clear choice for plasmid purification for transfectionapplications in research laboratories.Thermo Scientific GeneJET plasmid purification productsprovide molecular-grade purity at competitive priceswithout any reduction in quality. Designed for the budgetconscious, GeneJET kits offer reliable performance withsimple workflows.Which plasmid DNA isolation technology is right for you?Purity gradeMolecularTransfectionKitsGeneJET kitsEndotoxin levelStandard ( 10 EU/µg)Yield up to20 µg–1 mg20 µg–15 mg0.4–1.5 mg1.5–15 mgTechnologySilica membraneAnion exchange (resin)Advanced silica membraneAnion exchange (membrane)Total protocol time15–60 min30–120 min15–30 min90–120 minPrep sizeMini–maxiMini–gigaMidi maxiMaxi, mega, and gigaDownstreamapplicationNot sensitive PCRSensitive Standard transfectionsVery sensitive Primary and stem cell transfection Nucleic acid labeling All molecular biology applications Cloning(digestion, ligation) In vitro transcription Gene therapy and vaccine(in vivo) researchPureLink HiPure kitsPureLink Fast Low-Endotoxinkits ( 1 EU/µg)Low endotoxin (0.1–1 EU/µg) SequencingFind out more at thermofisher.com/plasmidprep24Advanced transfectionPureLink ExpiEndotoxin-Free kitsEndotoxin-free ( 0.1 EU/µg) Microinjection All molecular biology applications

Which plasmid purification solution is right for your downstream application?PureLink HQ MiniPlasmid DNAPurification KitPureLink ExpiEndotoxin-FreeMaxi, Mega,and Giga cing)AdvancedtransfectionLow-to-medium throughputPureLink ProQuick96Plasmid KitHigh throughput (96-well format)Low throughputSpin/vacuum column (silica)Downstreamapplication(Start here)TransfectionPureLink QuickPlasmid Miniprep KitAdvanced silica(vacuum-assisted)Anion-exchange gravity-flowcolumn and/or filterPureLink FastLow-EndotoxinMidi and MaxiPlasmidPurification KitsGeneJET PlasmidMiniprep, Midiprep,and Maxiprep KitsLow throughputAnion-exchange(vacuum-assisted)PureLink HiPurePlasmid FilterMidiprep andMaxiprep KitsPureLink HiPureExpi PlasmidMegaprep andGigaprep KitsPureLink HiPurePlasmid FP (Filterand Precipitator)Maxiprep KitPlasmid DNA purification products, prep sizes, and approximate yieldsPrep sizeOvernight bacterial culture volumeApproximate plasmid yieldMiniprep1–5 mLUp to 40 µgMidiprep10–50 mLUp to 300 µgMaxiprep100–200 mLUp to 1 mgMegaprep500 mL–2.5 LUp to 5 mgGigaprep2.5–5 LUp to 15 mg25

Low-endotoxin plasmid DNA purificationFast advanced silica technologyInvitrogen PureLink Fast low-endotoxin plasmidpurification kitsAdvanced silica membrane technology dramaticallyreduces protocol time to as little as 15–30 minutes.This next-generation methodology requires no alcoholprecipitation step, resulting in a short and robustworkflow. Used in combination with a vacuum manifold,the advanced silica membranes significantly shortenpurification times. This technology helps provide highquality, low-endotoxin plasmid DNA suitable for transfectionof robust cell lines and applications such as PCR, cloning,and sequencing.High-quality, low-endotoxin DNAObtain plasmid with less than 1 EU/μg of endotoxin,ideal for standard transfections and all molecularbiology applications (PCR, cloning, sequencing, etc.)Achieve inherently low endotoxin levels.Colored buffersWith their easy-to-follow plasmid purification protocol,colored buffers are offered as an added conveniencethat promote visualization and identification of completebacterial cell lysis and neutralization.High yieldsIsolate up to 0.4 mg (midi) or 1.5 mg (maxi) of high-qualityplasmid DNA (Figure 5).MidiPureLink Fast kitMaxiQiagen Plus kitPureLink Fast kitQiagen Plus kit0.51.5 1.0 EU/µgYield (mg)Yield (mg)0.4 0.05 EU/µg 1.0 EU/µg0.30.21.2 0.05 EU/µg0.90.60.10.301Plasmid201Plasmid2Figure 5. Achieve high yields of low-endotoxin, advanced transfection–quality plasmid DNA with PureLink Fast kits when compared to kitsfrom another supplier. Two high-copy plasmids with different backbones were purified using PureLink Fast and Qiagen Plasmid Plus midiprep andmaxiprep kits as described in the product manuals. Data for plasmid yields are shown in the graph. Endotoxin values (EU/μg) were measured usingEndosafe-PTS instrument (Charles River); values are provided only for PureLink Fast preparations.Find out more at thermofisher.com/lowendoplasmid26

Endotoxin-free plasmid DNA purificationFast, easy-to-use, and cost-effective kits for sensitive downstream applicationsInvitrogen PureLink Expi Endotoxin-Free pl

Magnetic beads for DNA purification 9 Genomic DNA purification kits 10 Genomic DNA extraction 16 Genotyping—pharmacogenomics studies 17 Plant genomic DNA isolation kits 18 Viral genomic DNA purification kits 20 Genomic DNA from saliva 21 Complete purification system for nucleic acids