Epigenase 5-mC Hydroxylase TET Activity/Inhibition Assay Kit
EPIGENTEKComplete Solutions for EpigeneticsEpiMag HT (96-Well) Magnetic SeparatorBase Catalog # Q10002INTENDED USEEpiMag HT (96-Well) Magnetic Separator allows paramagnetic bead precipitation of liquid samples fromvarious 96-Well microplates. It can be used for isolation and purification of nucleic acids and proteins,immunoprecipitation, immunoassays (ELISA), cell sorting, and purification of biomolecules. With use of the HT(96-Well) magnetic separator adapter (included with separator), this magnetic separator can also be used formost PCR microplates.110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735Tel: 1-877-374-4368 Fax: 1-718-484-3956 E-mail: info@epigentek.com Web: www.epigentek.com Epigentek Group Inc. All rights reserved. Products are for research use only.Page 1Printed 2015-06-04Q10002
EPIGENTEKComplete Solutions for EpigeneticsPRODUCT CONTENTSComponentCat. #Q10002StorageUpon Receipt96-Well Magnetic SeparatorMagnetic Separator Adapter96-Well MicroplateAdhesive Cover FilmUser Guide11111RTRTRTRTRTSHIPPING & STORAGEThe product is shipped at ambient temperature.Upon receipt: Store this magnet at room temperature and a safe distance from magnetic storagedevices such as hard disks and cards with magnetic stripes, such as credit cards. Keep this magnetaway from electronic devices and persons with cardiac pacemakers.GENERAL PRODUCT INFORMATIONQuality Control: EpiMag HT (96-Well) Magnetic Separator is tested against predeterminedspecifications to ensure consistent product quality. Epigentek guarantees the performance of allproducts in the manner described in our product instructions.Product Warranty: If this product does not meet your expectations, simply contact our technicalsupport unit or your regional distributor. We also encourage you to contact us if you have anysuggestions about product performance or new applications and techniques.Safety: Suitable lab coat, disposable gloves, and proper eye protection are required when workingwith this product.Product Updates: Epigentek reserves the right to change or modify any product to enhance itsperformance and design. The information in this User Guide is subject to change at any time withoutnotice. Thus, only use the User Guide that was supplied with the product when using that product.Usage Limitation: The EpiMag HT (96-Well) Magnetic Separator is for research use only and is notintended for diagnostic or therapeutic application.Intellectual Property: The EpiMag HT (96-Well) Magnetic Separator and methods of use containproprietary technologies by Epigentek.PRODUCT DESCRIPTIONThe implementation of twenty-four extremely powerful magnetic rods made of neodymium-iron boronenables fast and easy magnetic separation. Each magnetic rod addresses four wells of the 96-well plate.With this magnetic separator, paramagnetic beads will be firmly pulled to the side of the plate wells, whichensures complete removal of the magnetic beads from the solution by a robot or with a multichannelpipette, minimizing sample loss. This product has the following features:110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735Tel: 1-877-374-4368 Fax: 1-718-484-3956 E-mail: info@epigentek.com Web: www.epigentek.com Epigentek Group Inc. All rights reserved. Products are for research use only.Page 2Printed 2015-06-04Q10002
EPIGENTEKComplete Solutions for Epigenetics Ultra-Powerful NdFeB Magnets - allows for the fastest separation times and minimal beadloss. Efficient Particle Washing - Vertical orientation of the bar magnetic field attracts andsuspends the magnetic particles both to the side and up from the bottom of the wells. Pipettetips can be inserted to the bottom of the well, insuring thorough and efficient washing andremoval of fluids. Maximum Sample Concentration - Samples can be efficiently recovered from 10 µl to 2 mlof liquid. Universal Compatibility – Suitable for most magnetic beads or particles including magneticiron oxide nanoparticles and magnetic agarose beads (e.g., EpiNext beads, AMPure XP,Axygen, etc). Compatible with all microtiter plates, including standard 96-well plates (0.25 to0.3 ml wells) and deep well plates (1 to 2 ml) with various well shapes (U-shape, V-shape,and flat bottom). The included adapter allows for use with various skirted and unskirted PCRmicroplates. Lightweight Handheld Design - Allows for easy repeated handling.PROTOCOL OF USEThe working procedure is described as the follows:Fig 1. Workflow for using EpiMag HT (96-well) Magnetic Separator forsample molecule isolation110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735Tel: 1-877-374-4368 Fax: 1-718-484-3956 E-mail: info@epigentek.com Web: www.epigentek.com Epigentek Group Inc. All rights reserved. Products are for research use only.Page 3Printed 2015-06-04Q10002
EPIGENTEKComplete Solutions for EpigeneticsStandard or Deep-Well Microplate1.Add the sample solution into a microplate (if the microplate does not contain sample solution),then add appropriate binding buffer (if necessary) and magnetic beads into the plate for anappropriate incubation time.2.Place the microplate onto the HT (96-Well) Magnetic Separator. The magnetic beads willaggregate to the side or bottom of the well. The time period of complete capture of the beads isdependent on the solution volume and size of the beads, ranging from several seconds to severalminutes.3.Remove the fluid completely with a pipette and wash the beads with appropriate wash solutionseveral times. Leave the plate on the separator for 1-4 min to to ensure optimal removal of anyresidual fluid.4.Elute the sample molecules from the beads with appropriate elution buffer.PCR MicroplatesNote: PCR microplates have narrow wells which increases surface tension and makes removing theexcess fluid of the original sample from the beads difficult. For the best results, we recommendtransferring the sample solution in the PCR plate into the 96-well microplate included with the separatoror a standard microplate.If PCR microplates have to be directly used, an HT (96-Well) Magnetic Separator Adapter is required(included with the separator). A skirted PCR plate is considered to best fit the separator.1.Add the sample solution into a microplate (if the microplate does not contain sample solution),then add appropriate binding buffer (if necessary) and magnetic beads into the plate wells for anappropriate incubation time.2.Place the adapter onto the HT (96-Well) Magnetic Separator. Then, put the PCR onto theadapter.3.Slightly move the adapter (left or right direction against the separator) to bring the wells close tothe magnetic rods. The magnetic beads will aggregate to the side or bottom of the well. The timeperiod of complete capture of the beads is dependent on the solution volume and size of thebeads, ranging from several seconds to several minutes.4.Remove the fluid completely using a pipette and wash the beads with appropriate wash solutionseveral times. Leave the plate on the separator for 1-4 min to ensure optimal removal of anyresidual fluid.5.Elute the sample molecules from the beads with appropriate elution buffer.RELATED PRODUCTSBeads-based DNA Isolation and Clean-upP-1063The EpiNext DNA Purification HT SystemSonication InstrumentsEQC-1100EpiSonic Multi-Functional Bioprocessor 1100110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735Tel: 1-877-374-4368 Fax: 1-718-484-3956 E-mail: info@epigentek.com Web: www.epigentek.com Epigentek Group Inc. All rights reserved. Products are for research use only.Page 4Printed 2015-06-04Q10002
EPIGENTEKComplete Solutions for EpigeneticsQ10003EpiMag 96-Well MicroplateDNA Enrichment ReactionP-1015Methylamp Methylated DNA Capture (MeDIP) KitP-1038EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) KitP-1052EpiQuik MeDIP Ultra KitP-2002EpiQuik Chromatin Immunoprecipitation (ChIP) KitP-2003EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) KitP-2014EpiQuik Plant ChIP KitP-2025ChromaFlash One-Step ChIP KitP-2026ChromaFlash One-Step Magnetic ChIP KitP-2027ChromaFlash High-Sensitivity ChIP KitDNA Library PrepP-1051EpiNext DNA Library Preparation Kit (Illumina)P-1053EpiNext High-Sensitivity DNA Library Preparation Kit (Illumina)P-1055EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina)P-1059EpiNext DNA Size Selection Kit110 Bi County Blvd. Ste. 122, Farmingdale, NY 11735Tel: 1-877-374-4368 Fax: 1-718-484-3956 E-mail: info@epigentek.com Web: www.epigentek.com Epigentek Group Inc. All rights reserved. Products are for research use only.Page 5Printed 2015-06-04Q10002
Universal Compatibility –Suitable for most magnetic beads or particles including magnetic iron oxide nanoparticles and magnetic agarose beads (e.g., EpiNext beads, AMPure XP, Axygen, etc). Compatible with all micr
BTC (2014-15) 44.73% 55.26% nil 28. How many students have cleared national and state competitive examinations such as NET, SLET, GATE, Civil services, Defense services, etc.? Session Name of the Exam No. Of Students Enrolled/Appeared No. Of Students Qualified 2013-14 C-TET UP-TET UGC-NET 19 05 02 01 2014-15 C-TET UP-TET UGC
investigate the biological roles of TET enzymes in lineage-committed normal and cancer cells. To this end, murine primary cells with genetic deletion of TET enzymes and human cancer cells with recurrent mutations in the cofactor providing isocitrate dehydrogenases (IDH), provoking competitive inhibition of TET enzymes, were analyzed.
(Haitian/creole) Pwoteje Tet Ou Kont Pestisid . Gid Pou Travaye Agrikilti . The Environmental Protection Agency . revised the Worker Protection Standard for agricultural pesticides in August 1992. The revised Worker Protection Standard requires that agricultural workers be given training in basic pesticide safety. Protect Yourselffrom Pesticides: Guide for Agricultural Workers was developed by .
Characterization of TET and IDH gene expression in chronic lymphocytic leukemia: comparison with normal B cells and prognostic significance Michaël Van Damme1*, Emerence Crompot1, Nathalie Meuleman2, Marie Maerevoet2, Philippe Mineur3, Dominique Bron2, Laurence Lagneaux1 and Basile Stamatopoulos1 Abstract
isocitrate dehydrogenases (IDH) result in a substantial increase of 2-hydroxyglutarate, a co-factor that inhibits TET activity and could lead to a subsequent buildup of DNA methylation (Figueroa et al. 2010). Although these proteins are currently the subject of intense investigation, it is clear that the TET proteins play an essential role in the
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 257, No. 11, Issue of June 10, pp. 6605-6613, 1982 Printed in U.S.A. Purification of the TET Repressor and TET Operator from the Transposon TnlO and Characterization of Their Interaction* (Received for publication, September 16, 1981) Wolfgang Hillen, Gerd Klock, and Inge Kaffenberger From the Znstitut fir Organische Chemie und Bwchemie, Technische .
KERALA TEACHER ELIGIBILITY TEST (K-TET) 2012 State Council of Educational Research and Training (SCERT) KERALA August 2012 SYLLABUS Category IV [For Language Teachers in Arabic, Urdu, Sanskrit, Hindi (Up to Upper Primary Level), . std. V to XII. K
vocabulary materials such as graded readers, which go up through approximately the 4,000-word-family level, to more challenging texts such as newspapers, classic novels, and academic texts, at the .
