Titrette And Digital Burette
Titrette and Digital Burette(all models)Testing Instructions (SOP)May 20131. IntroductionThe standard ISO DIS 8655 describes both the design and thetesting of bottle-top burette. The following Testing Instructionsdescribe how to apply the ISO standard in practice.We recommend a testing every 3-12 months. This interval may beadjusted to individual requirements. For example, when workingvery frequently or when using aggressive media, the instrumentshould be tested more frequently.These Instructions may also be used as a basis for the supervisionof testing devices to DIN EN ISO 9001, DIN EN ISO 10012 andDIN EN ISO/IEC 17025.For the regular examinations required by DIN EN ISO 9001, DINEN ISO 10012, DIN EN ISO/IEC 17025 and the GLP Guidelines,BRAND additionally provides a calibration service (see page 9).Your instrument will be returned within a few days together witha test report. For more detailed information, please contact yourlabware supplier.
2. Preparation for testing and visual examination2.12.2Type and serial numbern Determine instrument type and nominal capacity. Enter/ check in Test Record (1).n Read Serial Number(embossed at the housing). Enter number in Test Record (1).n Read customer’s identification, if present. Enter identification in Test Record (1).Minimal configuration Titrette and Digital Buretten Burette Use only manufacturer’s original parts.n Titrating tuben Filling tuben Mounting tool for valves2.3Cleaningn Rinse instrument with cleaning solution. Select suitable cleaning solution according to the mediumThen rinse the instrument several times withwich was used.distilled water.n Wipe off the exterior of the Titrette andDigital Burette.2.4 Visual examination(Damage, leakages)n Housing Enter fillings in Test Record (2).n Titrating tuben Filling tuben Recirculation tuben Displayn Controlsn LeakagesPossible faults and resulting measures:FaultMeasuresFilling or titrating tube bent or damagedPossibl safety risk - therefore replace parts(see Operating Manual “Accessories“).LeakagesPossibl safety risk - therefore replace parts or return instrumentfor repair.Faulty controls, faulty displayReturn instrument for repair.2
2.5Functional testn Screw the Titrette / Digital Burette on a bottlefilled with distilled or deionised water(according to ISO 3696, at least quality 3).n Priming (see Operating Manual). A few bubbles up to 1 mm in the glass cylinder arepermissible.n Check controls Hand wheels operating smoothly Function of the keys On/Off, Pause and CLEAR.n Result Enter findings into Test Record (3)If there is a malfunction of the instrument (e.g., piston difficult to move, sticking valves or leakage) please consult the "Troubleshooting" section of the operating manual.3. Required equipment for testingn Titrette /Digital Buretten Bottle (at least 500 ml) filled with distiled or deionisedwater (according to ISO 3696, at least quality 3,room temperature). Match temperature of water and room.n Recipient (e.g., Erlenmeyer flask, narrow-mouth)filled with some water. Bottom of vessel should be covered.n Thermometer with a measuring error of maximum: 0.1 Cn Place the instrument into the testing roomfor at least 1 hour (unpacked!). Allow instrument to adjust to room temperature.n Balance, recommended specifications:Selected volume ofappartatus under testResulutionRepeatability andlinearityStandard uncertaintyof measurementVmgmgµl12225 ml V 50 mlTraceability of test results to national standardsThrough the use of calibrated testing devices (balance and thermometer), the requirement of DIN EN ISO 9001, DIN EN ISO 10012and DIN EN ISO/IEC 17025 to refer the test to the national standard is fulfilled. The calibration of the balance e.g. can be carried outeither by DAkkS calibration or official certification of the balance, or by calibrating the balance with appropiate weights that are tracedto the national standard. The calibration of the thermometer can also be carried out by DAkkS calibration or official certification, or bya comparison with thermometers that are traced to the national standard (under defined conditions).3
4. Gravimetric test1. Determine temperature of the water for testing. Enter temperature into Test Record (4).2. Fill the instrument to the upper stop („Fill“).3. Dispense 5 drops into a separate vessel and wipe offthe titrating tube.4. Set display to zero („Clear“).5. Place receiving vessel upon the balance. Tare thebalance.6. Place recipient under the titrating tube. Dispense the Dispense with both hands to facilitate smooth dispensingentire nominal volume without interruption (the titratingwithout interruption.tube should not touch the inner wall of the recipient ).7. Wipe titrating tube at the receiving vessel.8. Place receiving vessel upon the balance. Write downthe value. Enter weighing value into the Test Record (5).9. Tare the balance again.10. Repeat points 2 to 9 another ten times.11. Then carry out ten more weighings each by dispensing For partial volumes it is not necessary to refill each time50% resp. 10% of the nominal capacity.before dispensing.4
5. Evaluation of gravimetric test resultsThe values obtained by weighing during the gravimetric test areonly the mass values of dispensed volume without correction ofair buoyancy. In order to obtain the actual volume, an adjustmentcalculation to account for water density and air buoyancy mustbe carried out. To facilitate your calculations and evaluations, werecommend the use of the Windows-compatible calibration software EASYCAL from BRAND.The following calculations must be carried out:1. Mean weighing values:(Example for ten weighing values)x x2 x3 x4 x5 x6 x7 x8 x9 x10x 1102. Mean volume:V x·Z3. Standard deviation volume:(x - x)2 (x2 - x)2 (x3 - x)2 (x4 - x)2 .(x10 - x)2s Z 194. Accuracy:V - Vnominal value· 100A [%] Vnominal value For factor Z, see Table 1. Enter value into Test Record (6a). For factor Z, see Table 1 Enter value into Test Record (6b) Enter value into Test Record (6c)5. Coefficient of variation:CV [%] s · 100V Enter value into Test Record (6d)Comparison actual/nominal values:n Use the error limits per Table 2 and 3, or defineyour own error limits. Enter values into Test Record (6e, f)Result:If calculated values A [%] and CV [%] are smaller than or equalto the error limits, the instrument is in good working order.If the calculated values are larger than the error limits:n Verify if the above instructions have been carefullyfollowed step by step.n Observe the suggestions under "Troubleshooting"in the Operating Manual.n Calibrate the Titrette and Digital Burette according theinstructions in the Operating Manual.If these measures are not successful, we offer you thepossibility to have your instruments calibrated by theBRAND Calibration Service (see page 9).5
Possible volume faults and resulting measures:FaultPossible causesMeasuresVolume too largen Drop remaining on the titrating tube Before weighing, wipe off any drop intothe receiving vessel.Tare the balance.n Dispensed too fast or unevenly Repeat the test and dispense moreslowly.n Dispensing with media which formdeposits in the piston Clean the instrument with a suitableagent according to the medium used.n Filling valve/titrating tube leaking Tighten the filling valve/titrating tube(see Operating Manual) or clean it.Replace if necessary.n Instrument calibrated wrongly ( CAL appears on the display) Revert to original calibration (seeOperating Manual).n Jerky titration Turn wheels evenly and withoutexerting any pressure.n Temperature adjustment betweeninstrument, room and watertemperature not completed Carry out temperature adjustment.Volume too smallOther causesTable 1:Table 2:Excerpt from DIN EN ISO 8655Table refers to 1013 hPaThe validity range 980 hPa to 1040 hPaError limits for Titrette and Digital Burette:Temperature CFactor Zml/gTemperature CFactor 51.0052221.0033301.005422.51.0034The stated error limits refer to new instruments under optimizedtesting conditions (qualified operators and standardized ambienceconditions).Titrette bottle-top buretteVolumemlPartialvolume, mlA %10100.1050.2012550Digital Burette(all models)bottle-top buretteCV %µlA % µlCV .2252.50.70180.256250125500.06300.02100.2100 0.150250.12300.04100.4100 0.25050.60300.20102100 150µlFor calibration, the error limits to be observed by the operatormust be individually defined by the user. For this purpose, thefollowing methods can be applied: If required by the application and if the optimized conditions formeasuring are present, the error limits in Table 2 can also beexpected in the case of used volumetric instruments in goodworking order.Table 3:Excerpt from DIN EN ISO 8655, part 3NominalvolumeError margins forsystematic errorError margins forrandom errorml % [A] µl% [CV]µl [s]100.3250.2300.110500.1500.2251000.150Test:10 single measurements per test volume according to DIN EN ISO 8655.For definition of A, CV and s see sample calculation chapter 5.For checking of partial volumes the values for A [%] and CV [%] whichare related to the nominal volume have to be converted. In analogy to the German regulations for official testing, it isalso admissible to apply the limits which are typical for practice.These practice limits correspond to double the limits for officialtesting. In this case, the values found in Table 2 should bedoubled. The user may also define his own individual error limitscorresponding to his particular application, and apply thesetolerances for the calibration of his instrument.The above procedures fulfil the requirements of DIN ENISO 9001, DIN EN ISO 10012 and DIN EN ISO/IEC 170256
Test Record for Volumetric Instruments1. Instrument: Titrette Digital Burette Dispensette Transferpette Transferpette Transferpette electronic Transferpettor Type: fix analog digitalNominal capacity:Serial number:Customers identification:2. Damage: NoneType of damage:Damage repaired3. Functional defects: NoneType of functional defect:Functional defect repaired4.Water temperature: CBalance:Thermometer:5.Results of gravimetric testWeighing No.Nominal volume50 %10 %50 %10 %x1x2x3x4x5x6x7x8x9x106.Evaluation of gravimetric testProcedureaVbscA [%] FounddCV [%] FoundeA [%] NominalfCV [%] NominalgresultNominal volumeThe testing was carried out according to DIN EN ISO 8655.DateSignature7
6. Declaration on the Absence of Health HazardsTo be sent together with the instruments or via Mail (if urgent by Fax in advance).ToBRAND GMBH CO KGOtto-Schott-Straße 2597877 WertheimFax: 09342 808-91290We intend to give our staff a maximum of protection from health hazards caused by contaminated instruments. We therefore ask foryour understanding that we cannot carry out any calibration / repair unless this declaration is submitted completed and signed.Re: Instrument Consignment dated/ for Delivery Note no.The Undersigned hereby declares:n That the instruments have been carefully cleaned and decontaminated before shipment.n That the instruments pose no danger through bacteriological, chemical, radiological or viral contamination.n To be authorised to make declarations on behalf of the Institution represented.n For calibrating service only: minor repairs of a value up to 25,- VAT will be carried out and invoiced without further queries(cross out if not applicable).Company / Laboratory (Stamp)NamePositionDate, SignatureTel. / Fax / E-Mailn In case of Return for Repair, please provide us with the following supplementary information:Detected defectMedia which the instrument has been used with:8
7. Calibration Service from BRANDBRAND offers a full service including calibration and adjustment of Brand- and foreign instruments as well as maintenance andrepair if necessary - only for BRAND- instruments. This saves money and adds the benefit of an independent review organisation for the calibration of the instruments. Further information and the order form for repair- and calibration service are found onwww.brand.de.7.1Range of instruments covered1. Piston-operated pipettes (single- and multichannel)2. Bottletop dispensers3. Piston burettes (bottle-top burettes)4. Hand dispensers7.2Testing according to DIN EN ISO 8655At BRAND, a team of qualified staff, working in temperature and humidity controlled rooms using the most modern balancesand calibration software, calibrates Liquid Handling instruments, regardless of their make, according to DIN EN ISO 8655.Instruments with adjustable volumes such as HandyStep electronic, Transferpette , Transferpette S, Transferpette electronic, Transferpette S -8/-12, Transferpette S -8/-12. Transferpettor or Dispensette , Digital Burette or Titrette are testedat nominal volume, and at 50%, 10% or 20% of nominal volume.To document the results, a detailed test report is compiled which meets the requirement of different testing procedures.The BRAND Calibration Service offers:1. Calibration of Liquid Handling instruments, regardless of their make2. Detailed calibration certificate3. Return of instrument within a few working days4. Cost-efficient handling9
8. EASYCAL Software – advanced calibration technology8.1For liquid handling instruments and glass or plastic volumetric instrumentsEASYCAL simplifies the tedious task of calibrating liquid handling instruments and glass or plastic volumetric instruments to DIN EN ISO 9001,DIN EN ISO 10012, DIN EN ISO/IEC 17025 and GLP standards. Theprocedures are outlined step-by-step, and all calculations are performedautomatically. Reports are generated to document the calibration. All youneed is an analytical balance, a PC Windows 98/2000, NT (SP6), XP,Vista, 7, printer (optional) and EASYCAL software.n Suitable for instruments from all manufacturers.n Specifications of many instruments preloaded.n Testing according to ISO 4787, ISO 8655, etc.8.2Data Entryn Connect PC and balance (optional), then start the EASYCAL software.n 100 common balances are preprogrammed for ease of installation.8.3Documentation – clearly arrangedThe calibration certificate contains all important test data on one page.10
9. DAkkS-Calibration Service for Volumetric Instruments at BRAND9.1DAkkS – Deutsche Akkreditierungsstelle GmbH and DKDThe German Calibration Service (DKD) was foundedin 1977 as a joint task of state and economy andconstitutes the link between the measuring equipment in industrial and research laboratories, testing institutions and authorities and the national standards of the PTB (theGerman Institute of Physics and Metrology). It effectively supplements theexisting verification system which serves above all the purposes of consumerprotection. Based on the legal requirements the DKD Accreditation wassuccessively transformed to the DAkkS Accreditation (Deutsche Akkreditierungsstelle GmbH), starting from 2010. BRAND has been accredited bythe DAkkS since Apr. 23, 2013, with the certificate number D-K-1857201-00.9.2DAkkS-Calibration Certificate and Calibration SymbolThe DAkkS-Calibration Certificate documents officially on a high level the traceability of measuring results to national and international standards and to international SI-units, as required by standards as DIN EN ISO 9001 and DIN EN ISO/IEC 17025for monitoring of measuring devices.DAkkS-Calibration Certificates are issued when calibrations of an accredited laboratory are requested, when high level calibrations are necessary, when national and international standards are demanded and when reference instruments have to becalibrated.9.3DAkkS – A member in the International Accreditation NetworkDAkkS is a member of the International Laboratory Accreditation Cooperation (ILAC), the highest level international institution for laboratory calibration, and is a signatory to the MRA – Mutual Recognition Agreements.The accreditation bodies that are signatories to the ILAC mutual recognition agreements (MRAs) recognize their mutualequivalence, and the equivalence of the calibration certificates issued by those same signatories. Likewise, signatories areobliged generally to promote and recommend recognition of the calibration certificates of other signatories (excluding factorycalibration certificates).The DAkkS is a member of the EA (European Co-operation for Accreditation), which again is a member of the ILAC(International Laboratory Accreditation Cooperation). A multilateral agreement assures obligatory recognition of the DAkkScalibration certificate in a variety of countries.9.4DAkkS-Calibration Laboratory at BRANDIn 1998 a calibration laboratory for volumetric instruments at BRAND has been accredited by the German Calibration Serviceaccording to DIN EN ISO/ IEC 17 025. Our calibration laboratory is therefore authorized to issue DAkkS-Calibration Certificates (in several languages) for the volumetric instruments listed below. Furthermore we offer adjustment and – for BRANDLiquid Handling instruments – repair and maintenance.For ordering information on DAkkS-Calibration Certificates for volumetric instruments please consult our GeneralCatalog.9.5Volumetric instruments for which BRAND issues DAkkS Calibration CertificatesBRAND calibrates the following volumetric instruments (new or already in use and regardless of their make):n Piston-operated pipettes, from 0.1 µl to 10 mln Multichannel piston-operated pipettes, from 0.1 µl to 300 µln Piston-operated burettes, from 5 µl to 200 mln Dispensers, Dilutors, from 5 µl to 200 mln Volumetric instruments of glass, calibrated to contain (TC, In) from 1 µl to 10000 mln Volumetric instruments of glass, calibrated to deliver (TD, Ex) from 100 µl to 100 mln Volumetric instruments of plastic, calibrated to contain (TC, In) from 1 ml to 2000 mln Volumetric instruments of plastic, calibrated to deliver (TD, Ex) from 1 ml to 100 mln Density bottles of glass, from 1 cm3 to 100 cm311
0513/3BRAND GMBH CO KG · P.O. Box 11 55 · 97861 Wertheim · GermanyTel.: 49 9342 808-0 · Fax.: 49 9342 808-98000 · E-mail: info@brand.de · Internet: www.brand.de
of testing devices to DIN EN ISO 9001, DIN EN ISO 10012 and DIN EN ISO/IEC 17025. For the regular examinations required by DIN EN ISO 9001, DIN EN ISO 10012, DIN EN ISO/IEC 17025 and the GLP Guidelines, BRAND additionally provides a calibration service (see page 9). Your instrument will b
- Lab Coat - Sheet of White and Dark Paper Method 1. Rinse the tools (burette, flasks, and beakers). 2. Make sure the burette stopcock is closed. 3. Fill the burette with 25 mL of sodium hydroxide. 4. Place a beaker under the burette. 5. Open the stopcock to allow the liquid to drain out into the beaker and then close the stopcock.
pure substance. Safety and hazards. N Goalby chemrevise.org 2 . Core Practical 3: Make up a volumetric solution and carry out a simple acid–base titration. N Goalby chemrevise.org 6 Using the burette The burette should be rinsed out with substance that will be put in it
5. Add about 1 mL of iodate from the burette into a 25-mL Erlenmeyer flask. Record the final burette reading. 6. Add the following reagents into the above Erlenmeyer flask: a. 5 mL of deionized water b. Six drops of 2 M H 2SO 4 c. 2 mL of 10% KI 7. The solution in the Erlenmeyer flask should be reddish brown in color. Begin
Dispositif de niveau de ligne Etiquette jaune pour les sites d’accès Système d’attache sur la potence Burette en entonnoir avec ouverture à grand diamètre et robinet . Robinet de la ligne patient et de burette Poche de recueil de 700 mL valve anti-retour réliée distal du robinet patient.
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paper (if used) should be placed inside the front cover of the answer book. Rough work should be scored through. All questions should be attempted. Candidates should note that Question 10 contains a choice. Question 1 is on Pages 10, 11 and 12. Question 2 is on Page 13. Pages 12 and 13 are fold-out pages. NATIONAL QUALIFICATIONS 2014 FRIDAY, 16 MAY 1.00 PM – 3.30 PM [X274/13/02] Page two .
