In-Lab Training Manual

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In-Lab Training ManualUniCel DxC SynchronClinical SystemsFor In Vitro Diagnostic UseThis manual is intended forUniCel DxC 600UniCel DxC 800Rev. A (June 2014)Beckman Coulter, Inc.250 S. Kraemer Blvd.Brea, CA 92821 U.S.A.

UniCel DxC 600/800 Synchron Clinical SystemsWARNINGS AND PRECAUTIONSRead all product manuals and consult with Beckman Coulter-trained personnel beforeattempting to operate the instrument.Beckman Coulter, Inc. urges its customers and employees to comply with all nationalhealth and safety standards such as the use of barrier protection. This may include,but is not limited to, protective eyewear, gloves, suitable laboratory attire whenoperating or maintaining this or any other automated laboratory equipment.INTENTION FOR USEThis document is not intended to replace the information in your InstrumentInstructions For Use Manual (IFU). Information in the Instructions For UseManual supersedes information in any other manual.REVISION STATUSRev. A (June 2014)Based on DxC software version 5.4TRADEMARKSBeckman Coulter, the stylized logo, Access, Microtube, Synchron, and UniCel aretrademarks of Beckman Coulter, Inc., and are registered with the USPTO. All othertrademarks are the property of their respective owners.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 2 of 58Beckman Coulter Professional Development

UniCel DxC 600/800In-Lab Training ManualTable of ContentsChapter 1 Instrument Overview . 5Introduction . 5System Components . 5Sample Processing Summary . 8Chapter 2 Software Overview . 9Introduction . 9DxC 600/800 Main Menu . 10Chapter 3 Daily Start Up . 15Introduction . 15DxC Reagents . 15Flowcharts . 15DxC 600/800 Daily Start Up Routine . 16Chapter 4 Sample Processing . 27Sample Racks . 27Closed Tube Sampling (CTS) . 28Non-Reserved Racks. 28Reserved Racks . 29Processing Samples . 32Chapter 5 DxC 600/800 Instrument Commands . 35Instrument Commands . 35Shutdown and Power Up . 38Resources . 43In-Lab Training Checklist. 45UniCel DxC 600/800 Competency Exercise . 49UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 3 of 58Beckman Coulter Professional Development

UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 4 of 58Beckman Coulter Professional Development

Chapter 1Instrument OverviewIntroductionThe UniCel DxC Synchron Clinical System is an automated,computer-driven, general chemistry analyzer designed for the in vitrodetermination of a variety of general chemistries, therapeutic drugs,and other chemistries. Analysis can be performed on serum, plasma,urine, cerebrospinal fluid (CSF), pre-treated whole blood or wholeblood (sample type is chemistry dependent). DxC 600 and DxC 800systems are available. The DxC 800 offers additional STATchemistries.DxC 600DxC 800System ComponentsBoth the DxC 600 and DxC 800 can be divided into the followingcomponents: Sample Handling SystemModular Chemistry (MC) SystemCartridge Chemistry (CC) SystemHydropneumatic SystemOperation and Control ComponentsUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 5 of 58Beckman Coulter Professional Development

Sample Handling SystemThe DxC sample handling system is used to deliver samples to theModular Chemistry and Cartridge Chemistry systems. The samplehandling system is used to: load samples onto the system, identifysample programming by bar code ID or manual programming,position the samples for pipetting and analysis, and remove thesamples when they are no longer needed by the system. An optionalCTS (Closed Tube Sampling) cap piercing system allows loading andpiercing of validated sample container caps.Modular Chemistry (MC) SystemThe Modular Chemistry (MC) System performs rapid analysis of themore commonly ordered, high volume analytes.Both the DxC 600 and DxC 800 systems offer rapid analysis ofsodium, potassium, chloride, carbon dioxide, and total calcium.These chemistries are performed by an Ion Selective Electrode (ISE)module consisting of an Electrolyte Injection Cup (EIC), a flow cell,and electrodes specific for each analyte.DxC 600 systems have one cup module for the analysis of glucose.DxC 800 systems have 6 individual cup modules for the analysis ofglucose, BUN/UREA, creatinine, phosphorus, albumin, and totalprotein.Each reaction cup module has a precision pump to deliver reagent tothe reaction cup. The MC sample probe and syringe deliver sampleto the cup where it is mixed with reagent by a stir bar. The detectionsystem is chemistry dependent but will detect either light transmittedor electrical changes.Bulk reagents for ISE chemistries and cups are stored in the reagentstorage area. Alkaline Buffer reagent for the CO2 analysis is stored inthe ISE module.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 6 of 58Beckman Coulter Professional Development

Cartridge Chemistry (CC) SystemThe Cartridge Chemistry System provides random access analysis ofanalytes chosen by the user. Reagent cartridges are stored in arefrigerated compartment that houses up to 59 cartridges. Thereactions occur in permanent glass cuvettes that are housed in areaction carousel. Reagents are delivered to the reaction carousel byreagent probes using a reagent syringe. Sample is added by a CCsample probe using the CC sample syringe. Mixers are responsiblefor mixing the reagent and sample. Reactions are read by aphotometer assembly. A cuvette wash station cleans the cuvettes forreuse.Hydropneumatic SystemThe Hydropneumatic System is responsible for providing vacuum,compressed air, diluted wash solution, and deionized water for use onthe DxC 600/800 instrument. Wash Concentrate, No Foam and AutoGloss reagents are stored in this compartment.Operation and Control ComponentsBasic operating functions are controlled and reviewed by utilizing theDxC monitor, mouse, touch screen, keyboard, and push-buttoncontrols. These provide the interface between the operator and theDxC.Additional detailed information about DxC 600/800 components can be found inthe following References: UniCel DxC Synchron Clinical Systems Instructions For UseManualUniCel DxC Synchron Clinical Systems Reference ManualUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 7 of 58Beckman Coulter Professional Development

Sample Processing SummaryProgramming functions are performed, and then the sample rack isloaded onto the autoloader. The operator presses the green RUNbutton to begin the testing process. After the RUN button is pressed,the sample programming is identified. The CTS (Closed TubeSampling) will pierce the cap (if present) in preparation for the MCand CC sample probes. The rack is then loaded onto the SampleCarousel by the sample handling system.MC and CC processes occur simultaneously.If CC testing is required, the CC reagent probes, using the reagentsyringe, aspirate reagent from the CC reagent carousel into a cuvette.The reagent is mixed and warmed to reaction temperature, duringwhich time reagent blank readings are taken.The MC System begins MC testing (if required) by delivering reagentto the cup(s) and/or the ISE module. Sample is added by the MCsample probe using the sample syringe. The glucose sensordetermines the glucose concentration. The BUN electrodedetermines the BUN concentration while a colorimetric methodologyis used to determine the concentration of phosphorus, creatinine,albumin, and total protein. Ion selective electrodes in the flow celldetermine the concentration of each of the electrolytes.When the CC reagent has been warmed to the reaction temperature,the CC sample probe delivers sample to the cuvette containing thepre-warmed reagent. Measurements are taken by the photometer.The reaction readings are converted to concentration.The sample rack is off-loaded when it is no longer needed by thesystem.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 8 of 58Beckman Coulter Professional Development

Chapter 2Software OverviewIntroductionDxC 600/800 operating functions and programming functions areinitiated from the main operator screen at the DxC 600/800 analyzer.The screen provides status information to determine the present stateof the DxC system.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 9 of 58Beckman Coulter Professional Development

DxC 600/800 Main MenuNavigationThe DxC system provides three software navigation methods that canbe used to select an item on a screen or in a window:Touch a part of the screen using the tip of your finger or a touchdeviceUse the mouse to point the cursor and click the left mouse button toselect an itemPress a keyboard equivalent such as a function key, the enter key, orthe tab key to select an itemUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 10 of 58Beckman Coulter Professional Development

132StatusIndicatorsThe status of the system can be determined from any screen on theDxC monitor using the following indicators:1 CTSIndicator 2 HostCommunicationBar 3 SystemStatus Indicates when the Closed Tube Sampling is enabledA red circle with a slash through it indicates the CTS is notenabledShows communication status between host/DxCBlue/Green bar indicates information transferA red circle with a slash through it indicates the host and DxCare not communicatingIdentifies the operating status of the entire DxC systemOne status is displayed if both the MC and CC systems havethe same statusThe status information may be divided:Modular Chemistry (MC)/Cartridge Chemistry (CC)UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 11 of 58Beckman Coulter Professional Development

Menu BarA series of icons provide access to each of the major functional areas of theDxC 600/800 system. The background color of the function icons maychange to yellow or red to alert the operator when attention is required.MainView status information and perform main operatingfunctionsSamplesPerform sample programming functions including patientsand quality controlResultsRetrieve resultsRgts/CalView reagent and calibration status and perform reagentand calibration functionsQCDefine and manage Quality Control functionsSetupPerform Setup functionsUtilsPerform functions such as: priming, maintenance, anddiagnosticsStatusView hardware status informationInstr CmdHelpUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Initiate specific instrument commands such as: Home,Pause, and ShutdownView version information for the Instructions For Use(IFU) Manual. Access the IFU by using Alt F1 on thekeyboardPage 12 of 58Beckman Coulter Professional Development

12Sample Status The Main Menu Screen is used to determine the sample status foreach sample.Sample Status Icons (1) are displayed in front (2) of the Sample ID onthe Main Menu screen.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 13 of 58Beckman Coulter Professional Development

UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 14 of 58Beckman Coulter Professional Development

Chapter 3Daily Start UpIntroductionThe table below and a series of flowcharts will assist you insuccessfully performing the DxC 600/800 Daily Start Up.DxC ReagentsDxCReagentChangeduring arningsat 10%, 5%,and 0%volume0 testsavailable;ExpiredYesPop-upwarning at 5testsremaining incartridge0 wcharts(CC sidecan beRunning)StandbyorRunningThe Daily Start Up table lists all tasks necessary to bring theDxC 600/800 to operating condition for the start of a run.Refer to individual flowcharts on the following pages for instructionson each task.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 15 of 58Beckman Coulter Professional Development

DxC 600/800 Daily Start Up RoutineStep1TasksClear Manually Assigned RacksCheck Reagent Status and Load Reagents2 Check Reagent StatusLoad MC ReagentsLoad CC ReagentsUnload CC ReagentsCheck Calibration Status and Calibrate Chemistries3 Check Calibration StatusCalibrateProgram and Process Quality Control4 Process Quality Control using Auto Generation ofControlManually Program/Process Quality ControlUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 16 of 58Beckman Coulter Professional Development

Clear Manually Assigned RacksSelect Samples from the menu barSelect Clear F7Select the Rack(s) field. Type the range ofrack numbers(s) to be clearedNote: Enter 1-999 to clear all racksSelect Enterfrom the keyboardSelect OKSelect OKto confirmNotes about Clearing RacksClearing Racks does not clear programming for Sample ID’s; it onlyclears the assignment to a rack/position. Your Closed Tube TrackingDatabase tracks which samples have been pierced by Sample ID.You may get a message informing you that some racks are In-Progress,Removed, Requested for calibration, or Reserved for Cal. This featureprotects you from inadvertently clearing a rack that is needed for one ofthese conditions.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 17 of 58Beckman Coulter Professional Development

Check Reagent StatusSelect Rgts/Cal from the menu barSelect Print F10Note: Reagent status can also be viewed on the softwareReview the Vol (%)/Tests Left, Days Left,and Reagent Status columnsfor reagent requirementsUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 18 of 58Beckman Coulter Professional Development

Load MC ReagentsUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 19 of 58Beckman Coulter Professional Development

Load CC ReagentsPrepare reagent(s) if requiredRemove caps, remove any liquid adhering to the chimneys of thecartridge and remove any bubbles on the surface of the reagentSelect Rgts/Cal from the menu barPage down to locate the position(s) on the CC carousel wherereagent cartridge(s) will be placed (pages 3 to 8)Select all Positions to loadreagent cartridgesSelect Load F1When prompted by the software, open the reagent carousel doorPlace cartridge in the load position in front of the blinking barcode reader beamOREnter Reagent name (Chem)Using the picture on the right asa reference, enter the following:Lot number (numbers only)Serial numberExpiration date (MM/YY)Select the Tab key on thekeyboard to save the entriesVerify the reagent information is displayed on the monitorMove the cartridge onto the reagent carouselClose the reagent carousel doorAdditionalreagentsselected?YesNoProcedure completeUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 20 of 58Beckman Coulter Professional Development

Unload CC ReagentsSelect Rgts/Cal from the Menu barPage down to locate the position(s) on the CC carouselwhere reagent cartridge(s) will be unloaded (pages 3 to 8)Select all Positions tounload reagent cartridgesSelect Load F1When prompted by the software,open the reagent carousel doorGrasp the cartridge in the load position (located at theblinking bar code reader) and move it in front of the barcodereader beam or select Clear F1 to remove reagentinformation. Verify the reagent information is removed fromthe monitor. Remove the cartridgeClose the reagent carousel doorAdditionalreagentsselected?YesNoProcedure completeUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 21 of 58Beckman Coulter Professional Development

Check Calibration StatusSelect Rgts/Cal from the menu barSelect Print F10Note: Calibration status can also be viewed on the softwareReview the Cal Time Left and Cal Statuscolumns to identify reagents requiringcalibrationUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 22 of 58Beckman Coulter Professional Development

CalibrateUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 23 of 58Beckman Coulter Professional Development

Process Quality Control using Auto Generation of ControlUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 24 of 58Beckman Coulter Professional Development

Manually Program/Process Quality ControlSelect Samples from the menu barClear Control IDsSelect Clear F7Enter the Control ID(s) to be cleared in theSample ID(s) field*Select Enter on the keyboardSelect OKSelect OK to confirm*Clear multiple IDsby typing in eachControl IDseparated by acommaManually Program ControlType the Control ID in the Sample ID fieldSelect Enter on the keyboardOptional: Enter rack/position number if a bar codeis not availableSelect the chemistries to be runSelect Save F10Repeat steps to program another level, if necessaryPour the control material into sample cup(s). Nest thesample cup(s) in the appropriate bar code labeledtube(s) or place in assigned rack/position(s)Place the rack in the autoloader with the rack bar codefacing to the rightPress the RUN button on the instrumentWhen Quality Control is complete,review for acceptability (defined by laboratory policy)Note: If a manually programmed control is incomplete,the system will only try to run the incomplete tests thenext time you run the Control ID. Clear the Control IDto clear the incomplete QC sample andreset Auto Generation of ControlConsult references fortroubleshooting assistanceUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)NoQuality Controlacceptable?YesProcedure completePage 25 of 58Beckman Coulter Professional Development

UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 26 of 58Beckman Coulter Professional Development

Chapter 4Sample ProcessingSample RacksSample racks are available in multiple sizes and colors to assist inrack usage and organization for the operator: Available rack sizes: 13 x 75 mm, 13 x 100 mm, 16 x 75 mm,16 x 100 mm Available rack colors: blue, brown, green, gray, and purpleSample racks are configured in the DxC software. Racks aredesignated as Non-Reserved or Reserved. The system identifies therack designation by the rack bar code label.The following pages define Non-Reserved and Reserved racks andlist the validated sample containers available for processing sampleson the DxC.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 27 of 58Beckman Coulter Professional Development

Closed TubeSampling(CTS)The CTS Cap Piercer assembly (optional) pierces validated primarysample tube caps. The sample probes aspirate sample from the tubewithout the need for cap removal.Non-ReservedRacksValidated sample tubes in Non-Reserved racks are evaluated for thepresence of a cap. The DxC will pierce capped tubes in Non-Reserved racks Only validated caps should be used with the CTS option Uncapped tubes and sample cups may also be placed in Racks Primary sample tube with cap (1-Blade Thick CTS) Becton Dickinson VACUTAINER with HEMOGARD 13 x 75 mm 13 x 100 mm 16 x 100 mm Greiner VACUETTE 13 x 75 mm 13 x 100 mm Sarstedt S-Monovette (requires 1-Blade Narrow CTS) 15 x 92 mm 15 x 75 mm (requires rack PN A18642) Primary or secondary sample tube with cap removed 0.5 mL Sample Cup (PN 651412)placed directly on a rack (Dead Volume 40 µL) 2.0 mL Sample Cup (PN 652730 or 81902)placed directly on a rack (Dead Volume 360 µL)UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 28 of 58Beckman Coulter Professional Development

ReservedRacksReserved racks are optimized for use with low volume samples. Racks are identified by aRRsticker The DxC will not check tubes for caps The DxC will not pierce capped tubes Capped tubes must not be loaded and processed in Reservedracks Systems without a CTS installed can have all racks designatedas Reserved in the DxC softwareUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 29 of 58Beckman Coulter Professional Development

ValidatedSampleContainersforReservedRacksR UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)0.5 mL Sample Cup (PN 651412)nested in a 5 mL Transfer Tube(PN 979272)Dead Volume 50 µL2.0 mL Sample Cup (PN 652730or 81902) nested in a5 mL Transfer Tube (PN 979272)Dead Volume 150 µL1.0 mL Insert Cup(flat bottom – PN 81915)in 13x100 mm or 13x75 mm tubeDead Volume 250 µL1.0 mL Pediatric Insert Cup(conical bottom – PN 81916)in 13x100 mm or 13x75 mm tubeDead Volume 50 µL2.0 mL Insert Cup (PN 81917)in 16x100 mm tubeDead Volume 150 µLPage 30 of 58Beckman Coulter Professional Development

ValidatedSampleContainersforReservedRacks, SYNCHRON Microtube(PN 448774) Dead Volume 60 µLRcontinued 0.5 mL Sample Cup nested in aRed Metal Cup Insert(PN 476406) Dead Volume 20 µL Reserved Racks for HbA1c Reserved Racks for IBCTUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)BD Microtainer in aPediatric Tube Adapter(PN 472987)Use Primary Tube SampleTemplate for volume requirementsPage 31 of 58Beckman Coulter Professional Development

Processing SamplesSamplePreparationPrepare samples as follows prior to loading on the system: SampleProgrammingUse the UniCel DxC Synchron Clinical Systems Primary TubeSample Template to determine adequate sample volume in aprimary tube; verify all other samples meet sample container deadvolume requirementsRemove visible blood from the top of capped tubes with a cottontipped applicator moistened with deionized waterPlace primary sample tubes with or without caps, 0.5 mL samplecups, and 2.0 mL sample cups in Non-Reserved racksPlace all other sample containers in Reserved racksEnsure there are no bubbles in the sampleDo not overfill insert cupsEnsure that the bar code label is properly placed on the tube andis aligned so that it is visible through the slot in the rackEnsure the sample tube is seated correctly in the appropriate rackSamples may be processed using various programming methods onthe DxC 600/800 system. Samples may be programmed at theLIS/Host, Data Management system, or DxC 600/800. Samples maybe processed with or without bar codes. Use the instructions on thefollowing pages to process samples based on programming and barcode availability.Note: Additional programming features available include: batchmode, rerun, off-line dilution, serum index, manual ORDAC, etc.Detailed instructions for these options may be found in theUniCel DxC Synchron Clinical Systems Instructions For UseManual or the UniCel DxC Synchron Clinical Systems ReferenceManual.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 32 of 58Beckman Coulter Professional Development

Sample ProcessingStart HereNoYesRemove visible blood from the top ofcapped tubes using a cotton tippedswab moistened with DI waterValidatedCTS primarytube?*Adequatesamplevolumedetermined byPrimary TubeSampleTemplateAdequatesamplevolume*?Remove capNoUse the Low Volume SampleProcessing flowchartYesIs sampleprogrammed atthe LIS?NoSelect Samplesfrom the menu barYesSelect Samplesfrom the menu barNoIs there areadable barcode?Enter the Sample ID.Enter Rack and Position ifreadable bar code is not availableSelect chemistries to be runEnter the Sample ID. Enter Rackand PositionYesIf necessary, select STAT checkbox, Sample Type, SampleComment and/or Demog F2Select Next F10Place container in theappropriate rack. Ensurethat bar code label, ifpresent, is visible throughthe rack slot. Verify thetube is seated properlySelect Next F10Load the prepared rackson the autoloader with therack bar code facing to therightPress the RUN buttonon the instrumentUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 33 of 58Beckman Coulter Professional Development

Low Volume Sample ProcessingUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 34 of 58Beckman Coulter Professional Development

Chapter 5DxC 600/800 Instrument CommandsInstrumentCommandsThe DxC 600/800 instrument commands are used to control functions ofDxC instrument components. Instrument commands include:Home: Moves mechanical assemblies to their “home” positions and primes thesystemRecovers from a motion errorReturns the system from the Stopped state to StandbyPause: Prevents initiation of new or additional tests at the DxCTests in process are completedUse to return the system to Standby as soon as possibleShutdown: Use to properly reboot or shutdown the system to prevent datacorruption (failure to follow recommended procedure may result in datacorruption)Enable/Disable Modules: Use to enable/disable a single MC cup module, the MC ISE module, theentire MC side, the CC Photometer, the LPIA module, the CC lowerreagent carousel, or the entire CC side of systemAll programming for disabled modules is aborted. When a module isdisabled the system will not run, prime, perform diagnostics, ormaintenance procedures using that moduleThe system may disable modules automatically after certainunrecoverable error conditionsWhen a module is enabled, it will automatically primeUnload All Racks: Use to unload racks from the DxC 600/800 sample carouselUse when access to an on board sample(s) is requiredUse when the screen displays racks on the Main Screen but the racksare not on the sample carousel following a system errorOther instrument commands include: 3 Stop Print, 5 Pause Waste B, and6 Resume Waste B. Information about these commands may be found inthe UniCel DxC Synchron Clinical Systems Instructions For Use Manual.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 35 of 58Beckman Coulter Professional Development

HomeSelect Instr Cmd from the menu barSelect 1 HomeThe DxC system status changes to HomingNote: If only one side of the instrument (MCor CC) is Stopped, the Home commandoperates on the side that is Stopped.Homing is complete when theDxC system status displays StandbyUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 36 of 58Beckman Coulter Professional Development

PauseSelect Instr Cmd from the menu barSelect 2 PauseNote: The Pause command is availableonly if the system status is Running.Select one of the options:1 MC Only2 CC OnlyOR3 Both MC and CCThe DxC system status will change toPausing and return to Standby whenin progress tests are completedIMPORTANTWhen Pausing the system, cuvette washing stopsas soon as the last result is available. Cuvettesmay be left dirty. Do not request a Pause andleave the DxC sitting for a long period of timewithout washing the cuvettes.See the IFU for more details on when to usePause.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 37 of 58Beckman Coulter Professional Development

Shutdown and Power UpShutdown andPower UpThe DxC 600/800 system may require a Shutdown and Power Upwhen: Some parts replacement procedures require power to be turnedoffThe lab power goes offThe system is moved to a new locationRecommended by a BCI specialist or a reference fortroubleshooting purposesUse the flowcharts on the following pages when shutting down andpowering up the DxC 600/800 system.UniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 38 of 58Beckman Coulter Professional Development

ShutdownVerify the DxC system status isStandby, Stopped, or Instrument DownSelect Instr Cmd from the menu barSelect 4 ShutdownSelect OKNote: The Shutdown in Progress screenappears with the message “Please wait forfurther instructions. DO NOT TURN THEPOWER OFF.”Select Shutdown when the Select Reboot orShutdown dialog box appearsTurn OFF the power switch behind the farright door of the DxC instrument when“Shutdown Complete: It is now safe to rebootyour computer” appearsTurn OFF the power to the DxC computerUniCel DxC 600/800 In-Lab Training ManualRev. A (June 2014)Page 39 of 58Beckman Coulter Professional Development

Power UpTurn ON the power to the DxC computerTurn ON the power switch behind the far rightdoor of the DxC instrumentThe DxC system status displays Startupduring the boot-up routine

UniCel DxC 600/800 In-Lab Training Manual Page 11 of 58 Rev. A (June 2014) Beckman Coulter Professional Development Status Indicators The status of the system can be determined from any screen on the DxC monitor using the following indicators: 1 CTS Ind

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