Dispensette - BrandTech

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Dispensette Testing Instructions (SOP)May 20131. IntroductionThe standard ISO DIS 8655 describes both the design and thetesting of the bottle-top dispenser. The following Testing Instructionsdescribe how to apply the ISO standard in practice.We recommend a testing every 3-12 months. This interval may beadjusted to individual requirements. For example, when workingvery frequently or when using aggressive media, the instrumentshould be tested more frequently.These Instructions may also be used as a basis for the supervisionof testing devices to DIN EN ISO 9001, DIN EN ISO 10012 andDIN EN ISO/IEC 17025.For the regular examinations required by DIN EN ISO 9001, DINEN ISO 10012, DIN EN ISO/IEC 17025 and the GLP Guidelines,BRAND additionally provides a calibration service (see page 9).Your instrument will be returned within a few days together witha test report. For more detailed information, please contact yourlabware supplier.

2. Preparation for testing and visual examination2.12.2Type and serial numbern Determine instrument type and nominal capacity. Enter/ check in Test Record (1).n Read Serial Number(embossed at valve block). Enter number in Test Record (1).n Read customer’s identification, if present. Enter identification in Test Record (1).Minimal configuration Dispensette n Dispenser Use only manufacturer’s original parts.n Discharge tuben Filling tuben Mounting tool for valves2.3Cleaningn Rinse instrument with cleaning solution. Select suitable cleaning solution according to the mediumThen rinse the instrument again several timeswich was used.with distilled water.n Wipe off the exterior of the Dispensette . Unscrew piston and lift it out completely following theinstructions of the operating manual. Remove desposits outglass cylinder and piston.2.4 Visual examination(Damage, leakages)n Housing Enter fillings in Test Record (2).n Discharge tuben Filling tuben Controlsn LeakagePossible faults and resulting measures:FaultMeasuresFilling tube or discharging tube bent or damagedPossible safety risk - therefore replace part(see Operating Manual “Accessories“).Mechanically damaged connectionsPossible safety risk - therefore place parts or return instrumentfor repair.Faulty controlsReturn instrument for repair.2

2.5Functional testn Screw the Dispensette on a bottlefilled with distilled or deionised water(according to ISO 3696, at least quality 3).n Priming (see Operating Manual). A few bubbles up to 1 mm in the glass cylinder arepermissible.n Check the volume adjustment and that the dispensing The dispensing piston must slide smoothly in the dispensingpiston moves freely.cylinder. Enter findings into Test Record (3)n ResultIf there is a malfunction of the instrument (e.g., piston difficult to move, sticking valves or leakage) please consult the "Troubleshooting" section of the operating manual.3. Required equipment for testingn Dispensette n Bottle (at least 500 ml) filled with distiled or deionisedwater (according to ISO 3696, at least quality 3,room temperature). Match temperature of water and room.n Recipient (e.g., Erlenmeyer flask, narrow-mouth)filled with some water. Bottom of vessel should be covered.n Thermometer with a measuring error of maximum: 0.2 Cn Place the instrument into the testing roomfor at least 1 hour (unpacked!). Allow instrument to adjust to room temperature.n Balance, recommended specifications:aSelected volumea ofappartatus under testResulutionRepeatabilityand linearityStandard uncertainty ofmeasurementVmgmgµl50 µl V 1000 µl0,10,20,21 ml V 10 ml0,10,20,210 ml V 100 ml122For practical purposes, the nominal volume may be used to choose the balance.Traceability of test results to national standardsThrough the use of calibrated testing devices (balance and thermometer), the requirement of DIN EN ISO 9001, DIN EN ISO 10012and DIN EN ISO/IEC 17025 to refer the test to the national standard is fulfilled. The calibration of the balance e.g. can be carried outeither by DAkkS calibration or official certification of the balance, or by calibrating the balance with appropiate weights that are tracedto the national standard. The calibration of the thermometer can also be carried out by DAkkS calibration or official certification, or bya comparison with thermometers that are traced to the national standard (under defined conditions).3

4. Gravimetric test1. Determine temperature of the water for testing. Enter temperature into Test Record (4).2. Set instrument to nominal volume.3. Dispense some liquid into the separate vessel.Wipe off the drop on the discharge tube against the wallof the recipient.4. Place recipient upon the balance. Tare the balance.5. Place recipient under the discharge tube.6. Pull up the piston as far as it will go. Move slowly and steadily.7. Push the piston all the way down. Move slowly and steadily.8. Wipe dispensing tube at the receiving vessel.9. Place receiving vessel upon the balance. Write down the Enter weighing value into the Test Record (5).value.10. Tare the balance again.11. Repeat points 2 to 10 another ten times.12. Then carry out ten more weighings each by dispensing50% resp. 10% of the nominal capacity.4

5. Evaluation of gravimetric test resultsThe values obtained by weighing during the gravimetric test areonly the mass values of dispensed volume without correction ofair buoyancy. In order to obtain the actual volume, an adjustmentcalculation to account for water density and air buoyancy mustbe carried out. To facilitate your calculations and evaluations, werecommend the use of the Windows-compatible calibration software EASYCAL from BRAND.The following calculations must be carried out:1. Mean weighing values:(Example for ten weighing values)x x2 x3 x4 x5 x6 x7 x8 x9 x10x 1102. Mean volume:V x·Z3. Standard deviation volume:(x - x)2 (x2 - x)2 (x3 - x)2 (x4 - x)2 .(x10 - x)2s Z 194. Accuracy:V - Vnominal value· 100A [%] Vnominal value For factor Z, see Table 1. Enter value into Test Record (6a). For factor Z, see Table 1 Enter value into Test Record (6b) Enter value into Test Record (6c)5. Coefficient of variation:CV [%] s · 100V Enter value into Test Record (6d)Comparison actual/nominal values: Use the error limits per Table 2 and 3, or defineyour own error limits. Enter values into Test Record (6e, f)Result:If calculated values A [%] and CV [%] are smaller than or equalto the error limits, the instrument is in good working order.If the calculated values are larger than the error limits: Verify if the above instructions have been carefullyfollowed step by step. Observe the suggestions under "Troubleshooting"in the Operating Manual. Calibrate the Dispensette according the instructions in theOperating Manual.If these measures are not successful, we offer you thepossibility to have your instruments calibrated by theBRAND Calibration Service (see page 9).5

Possible volume faults and resulting measures:FaultPossible causesMeasuresVolume too largen Drop remaining on the titrating tube Before weighing, wipe off any drop intothe receiving vessel. Tare the balance.n Jerky dispensing. Dispense slowly and steadily.n During filling, a drop has already beenreleased. Move piston cautiously toward theupper stop.n Instrument not tight. Repeat the functional test valves mustbe tightened or replaced; dischargetube or filling tube must be mountedproperly.n Air bubbles in the instrument. Instrument must be primedn Jerky titration Use smooth gentle movements tooperate the piston upwards anddownwards. Approach the upper andlower stops slowly, so that no drops willfall off the dispensing tube.n Temperature adjustment betweeninstrument, room and watertemperature not completed Carry out temperature adjustment.Volume too smallOther causesTable 1:Table 2:Excerpt from DIN EN ISO 8655Table refers to 1013 hPathe validity range 980 hPa to 1040 hPaError limits for Dispensette :The stated error limits refer to new instruments under optimizedtesting conditions (qualified operators and standardized ambienceconditions).Temperature CFactor Zml/gTemperature CFactor 41.0038Type 5.5181.002518.5Nominal volumemlAccuracyValue 6e %Coefficient of variationValue 6f 26.51.0044Type analog and 100.5/1.0/50.1/0.2/122.51.0034For calibration, the error limits to be observed by the operatormust be individually defined by the user. For this purpose, thefollowing methods can be applied:Table 3:Excerpt from DIN EN ISO 8655, part 5NominalvolumemlError limits forsystematic error % [A]Error limits forrandom error µl% [CV]µl 0250.61500.250500.63000.21001000.66000.2200Test:10 single measurements per test volume according to DIN EN ISO 8655.For definition of A, CV and s see sample calculation chapter 5.For checking of partial volumes the values for A [%] and CV [%] whichare related to the nominal volume have to be converted. If required by the application and if the optimized conditions formeasuring are present, the error limits in table 2 can also beexpected in the case of used volumetric instruments in goodworking order. In analogy to the German regulations for official testing, it isalso admissible to apply the limits which are typical for practice.These practice limits correspond to double the limits for officialtesting. In this case, the values found in Table 2 should bedoubled. The user may also define his own individual error limitscorresponding to his particular application, and apply thesetolerances for the calibration of his instrument.The above procedures fulfil the requirements of DIN ENISO 9001, DIN EN ISO 10012 and DIN EN ISO/IEC 170256

Test Record for Volumetric Instruments1. Instrument: Titrette Digital Burette Dispensette Transferpette Transferpette Transferpette electronic Transferpettor Type: fix analog digitalNominal capacity:Serial number:Customers identification:2. Damage: NoneType of damage:Damage repaired3. Functional defects: NoneType of functional defect:Functional defect repaired4.Water temperature: CBalance:Thermometer:5.Results of gravimetric testWeighing No.Nominal volume50 %10 %50 %10 %x1x2x3x4x5x6x7x8x9x106.Evaluation of gravimetric testProcedureaVbscA [%] FounddCV [%] FoundeA [%] NominalfCV [%] NominalgresultNominal volumeThe testing was carried out according to DIN EN ISO 8655.DateSignature7

6. Declaration on the Absence of Health HazardsTo be sent together with the instruments or via Mail (if urgent by Fax in advance).ToBRAND GMBH CO KGOtto-Schott-Straße 2597877 WertheimFax: 09342 808-91290We intend to give our staff a maximum of protection from health hazards caused by contaminated instruments. We therefore ask foryour understanding that we cannot carry out any calibration / repair unless this declaration is submitted completed and signed.Re: Instrument Consignment dated/ for Delivery Note no.The Undersigned hereby declares:n That the instruments have been carefully cleaned and decontaminated before shipment.n That the instruments pose no danger through bacteriological, chemical, radiological or viral contamination.n To be authorised to make declarations on behalf of the Institution represented.n For calibrating service only: minor repairs of a value up to 25,- VAT will be carried out and invoiced without further queries(cross out if not applicable).Company / Laboratory (Stamp)NamePositionDate, SignatureTel. / Fax / E-Mailn In case of Return for Repair, please provide us with the following supplementary information:Detected defectMedia which the instrument has been used with:8

7. Calibration Service from BRANDBRAND offers a full service including calibration and adjustment of Brand- and foreign instruments as well as maintenance andrepair if necessary - only for BRAND- instruments. This saves money and adds the benefit of an independent review organisation for the calibration of the instruments. Further information and the order form for repair- and calibration service are found onwww.brand.de.7.1Range of instruments covered1. Piston-operated pipettes (single- and multichannel)2. Bottletop dispensers3. Piston burettes (bottle-top burettes)4. Hand dispensers7.2Testing according to DIN EN ISO 8655At BRAND, a team of qualified staff, working in temperature and humidity controlled rooms using the most modern balancesand calibration software, calibrates Liquid Handling instruments, regardless of their make, according to DIN EN ISO 8655.Instruments with adjustable volumes such as HandyStep electronic, Transferpette , Transferpette S, Transferpette electronic, Transferpette S -8/-12, Transferpette S -8/-12. Transferpettor or Dispensette , Digital Burette or Titrette are testedat nominal volume, and at 50%, 10% or 20% of nominal volume.To document the results, a detailed test report is compiled.The BRAND Calibration Service offers:1. Calibration of Liquid Handling instruments, regardless of their make2. Detailed calibration certificate3. Return of instrument within a few working days4. Cost-efficient handling9

8. EASYCAL Software – advanced calibration technology8.1For liquid handling instruments and glass or plastic volumetric instrumentsEASYCAL simplifies the tedious task of calibrating liquid handling instruments and glass or plastic volumetric instruments to DIN EN ISO 9001,DIN EN ISO 10012, DIN EN ISO/IEC 17025 and GLP standards. Theprocedures are outlined step-by-step, and all calculations are performedautomatically. Reports are generated to document the calibration. All youneed is an analytical balance, a PC Windows 98/2000, NT (SP6), XP,Vista, 7, printer (optional) and EASYCAL software.n Suitable for instruments from all manufacturers.n Specifications of many instruments preloaded.n Testing according to ISO 4787, ISO 8655, etc.8.2Data Entryn Connect PC and balance (optional), then start the EASYCAL software.n 100 common balances are preprogrammed for ease of installation.8.3Documentation – clearly arrangedThe calibration certificate contains all important test data on one page.10

9. DAkkS-Calibration Service for Volumetric Instruments at BRAND9.1DAkkS – Deutsche Akkreditierungsstelle GmbH and DKDThe German Calibration Service (DKD) was foundedin 1977 as a joint task of state and economy andconstitutes the link between the measuring equipment in industrial and research laboratories, testing institutions and authorities and the national standards of the PTB (theGerman Institute of Physics and Metrology). It effectively supplements theexisting verification system which serves above all the purposes of consumerprotection. Based on the legal requirements the DKD Accreditation wassuccessively transformed to the DAkkS Accreditation (Deutsche Akkreditierungsstelle GmbH), starting from 2010. BRAND has been accredited bythe DAkkS since Apr. 23, 2013, with the certificate number D-K-1857201-00.9.2DAkkS-Calibration Certificate and Calibration SymbolThe DAkkS-Calibration Certificate documents officially on a high level the traceability of measuring results to national and international standards and to international SI-units, as required by standards as DIN EN ISO 9001 and DIN EN ISO/IEC 17025for monitoring of measuring devices.DAkkS-Calibration Certificates are issued when calibrations of an accredited laboratory are requested, when high level calibrations are necessary, when national and international standards are demanded and when reference instruments have to becalibrated.9.3DAkkS – A member in the International Accreditation NetworkDAkkS is a member of the International Laboratory Accreditation Cooperation (ILAC), the highest level international institution for laboratory calibration, and is a signatory to the MRA – Mutual Recognition Agreements.The accreditation bodies that are signatories to the ILAC mutual recognition agreements (MRAs) recognize their mutualequivalence, and the equivalence of the calibration certificates issued by those same signatories. Likewise, signatories areobliged generally to promote and recommend recognition of the calibration certificates of other signatories (excluding factorycalibration certificates).The DAkkS is a member of the EA (European Co-operation for Accreditation), which again is a member of the ILAC(International Laboratory Accreditation Cooperation). A multilateral agreement assures obligatory recognition of the DAkkScalibration certificate in a variety of countries.9.4DAkkS-Calibration Laboratory at BRANDIn 1998 a calibration laboratory for volumetric instruments at BRAND has been accredited by the German Calibration Serviceaccording to DIN EN ISO/ IEC 17 025. Our calibration laboratory is therefore authorized to issue DAkkS-Calibration Certificates (in several languages) for the volumetric instruments listed below. Furthermore we offer adjustment and – for BRANDLiquid Handling instruments – repair and maintenance.For ordering information on DAkkS-Calibration Certificates for volumetric instruments please consult our GeneralCatalog.9.5Volumetric instruments for which BRAND issues DAkkS Calibration CertificatesBRAND calibrates the following volumetric instruments (new or already in use and regardless of their make):n Piston-operated pipettes, from 0.1 µl to 10 mln Multichannel piston-operated pipettes, from 0.1 µl to 300 µln Piston-operated burettes, from 5 µl to 200 mln Dispensers, Dilutors, from 5 µl to 200 mln Volumetric instruments of glass, calibrated to contain (TC, In) from 1 µl to 10000 mln Volumetric instruments of glass, calibrated to deliver (TD, Ex) from 100 µl to 100 mln Volumetric instruments of plastic, calibrated to contain (TC, In) from 1 ml to 2000 mln Volumetric instruments of plastic, calibrated to deliver (TD, Ex) from 1 ml to 100 mln Density bottles of glass, from 1 cm3 to 100 cm311

0513/7BRAND GMBH CO KG · P.O. Box 11 55 · 97861 Wertheim · Germany ·Tel.: 49 9342 808-0 · Fax.: 49 9342 808-98000 · E-mail: info@brand.de · Internet: www.brand.de

of testing devices to DIN EN ISO 9001, DIN EN ISO 10012 and DIN EN ISO/IEC 17025. For the regular examinations required by DIN EN ISO 9001, DIN EN ISO 10012, DIN EN ISO/IEC 17025 and the GLP Guidelines, BRAND additionally provides a calibration service (see page 9). Your instrument will b

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