Thermo Scientific Varioskan Flash - Thermo Fisher Scientific
Thermo ScientificVarioskan FlashUser ManualRev. 1.2
Thermo ScientificVarioskan FlashUser ManualRev. 1.2, Cat. no. N06354
CopyrightCopyright 2008 Thermo Fisher Scientific. All rights reserved. First edition published in 2006. Printed inFinland. Reproduction of the accompanying user documentation in whole or in part is prohibited.PatentsThe Varioskan Flash has an international patent and an international patent pending.Trademarks“Microtiter”, “SkanIt” and “Varioskan” are registered trademarks of Thermo Fisher Scientific.All other trademarks and registered trademarks are the property of their respective holders.DisclaimerThermo Fisher Scientific reserves the right to change its products and services at any time to incorporatetechnological developments. This manual is subject to change without prior notice as part of a continuousproduct development. Although this manual has been prepared with every precaution to ensure accuracy,Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting fromthe application or use of this information. This manual supersedes all previous editions.No liability for consequential damagesThermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability touse this product.Warranty statementThermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against defectiveparts and materials, including defects caused by poor workmanship, for a period of one year from the date ofdelivery. Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warrantyat no extra charge for materials and labor provided that the products were used and maintained in accordancewith Thermo Fisher Scientific’s instructions. The warranty is invalid if products have been misused or abused.For the warranty to be effective, the product must have been purchased either directly from Thermo FisherScientific or from an authorized Thermo Fisher Scientific distributor. The guarantee is not transferable to athird party without prior written approval from Thermo Fisher Scientific. This guarantee is subject to thefollowing exclusions: Any defects caused by normal wear and tear. Defects caused by fire, lightning, flood, earthquake, explosion, sabotage, war, riot, or any other occurrenceof the type listed above. Refurbished products that are subject to different warranty conditions.THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES,INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY ORFITNESS FOR A PARTICULAR PURPOSE. The seller is not liable for any loss or damage arising out of orin connection with the use of the product or other indirect damages. These warranty terms and conditions canbe obtained from your local Thermo Fisher Scientific dealer.Consumables are not included in the warranty.
About This User ManualIntended usersThis user manual has been written for the actual end user (for example,laboratory technician) and provides information on the ThermoScientific Varioskan Flash, including the installation and operatinginstructions.Read the manual in its entirety before operating the instrument.How to use thisuser manualThis user manual has been designed to give you the information youneed to: Review safety precautions Install the Varioskan Flash Use the Varioskan Flash in daily research use Perform basic cleaning and maintenance procedures Troubleshoot the instrument performanceThis user manual also describes all the features and specifications of theVarioskan Flash instrument. Refer to Chapter 6: “TechnicalSpecifications”.In Chapter 8: “Troubleshooting Guide” you will find explanations ofall error and warning messages and a problem-solving guide. The usershould be familiar with the contents of Chapter 5: “Maintenance”.For ordering information, refer to Chapter 9: “Ordering Information”.For moreinformationFor software-related issues, refer to the Thermo Scientific SkanIt Softwarefor Varioskan Flash User Manual (Cat. no. N06355). Both theinstrument and software manuals can be found in PDF format on theThermo Scientific SkanIt Software for Varioskan Flash installation CD.For the latest information on products and services, visit our worldwideWeb sites on the Internet oskanhttp://www.thermo.com/readingroomIn an effort to produce useful and appropriate documentation, weappreciate your comments on this user manual to your local ThermoFisher Scientific representative.Thermo Fisher ScientificThermo Scientific Varioskan Flash User Manual3
About This User ManualSafety symbols and markingsSafety symbolsand markingsSafety symbols andmarkings used onthe Varioskan FlashThese symbols are intended to draw your attention to particularlyimportant information and alert you to the presence of hazards asindicated.The following symbols and markings appear on the type label and theinstrument itself.Power ON Power OFF Serial number Catalog number Date of manufacture Consult instructions for use Risk of radiation injury WEEE symbol This product is required to comply with the EuropeanUnion’s Waste Electrical & Electronic Equipment (WEEE) Directive2002/96/EC. Warning To avoid stray light, use blind plugs to close emptydispensing positions! A black label with the following text (Figure 2–3):CAUTION: WARNING: DISCONNECT SUPPLY BEFORESERVICING and AVERTISSEMENT: COUPERL'ALIMENTATION AVANT L'ENTRETIEN ET LEDEPANNAGE. 4 Thermo Scientific Varioskan Flash User ManualThermo Fisher Scientific
About This User ManualSafety symbols and markingsWarning and othermarkings used inthe documentationThe following symbols and markings appear in this user manual.Warning Risk of electric shock. Warning Biohazard risk. Warning Hot surface, risk of burns. Warning Risk of injury to the user(s). Warning Risk of ultraviolet radiation injury. Caution Risk of damage to the instrument, other equipment or loss ofperformance or function in a specific application. Note Marks a hint, important information that is useful in theoptimum operation of the system, or an item of interest. Thermo Fisher ScientificThermo Scientific Varioskan Flash User Manual5
About This User ManualInstrument safety and guidelines for useInstrument safetyand guidelinesfor use1. Always follow basic safety precautions when using the VarioskanFlash to reduce the risk of injury, biohazardous contamination, fire,or electrical shock.2. Read this user manual in its entirety prior to operating theinstrument. Failure to read, understand, or follow the instructionsin the manual may result in damage to the instrument, injury tolaboratory and operating personnel or poor instrumentperformance.3. Observe all “Warning”, “Caution”, and “Note” statements as well assafety symbols and markings on the instrument and in thedocumentation.4. Never open any other covers of the Varioskan Flash than thedispenser sliding cover (Figure 2–2) or measurement chamber door(Figure 2–2) while the instrument is plugged into a power source.5. Never open the measurement chamber door while the instrument isbusy (when the LED indicator is orange).6. You can push in the tray manually only when the instrument isswitched off.7. Never force a microplate into the instrument.8. The Varioskan Flash is intended for laboratory research use only.Observe proper laboratory safety precautions, such as wearingprotective clothing and following approved laboratory safetyprocedures. It is recommended that Good Laboratory Practices(GLP) are followed to guarantee reliable analyses.9. Preventative maintenance instructions should be followed closely tokeep the instrument in the best condition for maximum reliability.A poorly maintained instrument will not give the best results.6 Thermo Scientific Varioskan Flash User ManualThermo Fisher Scientific
ContentsIntended users . 3How to use this user manual . 3For more information . 3Safety symbols and markings. 4Safety symbols and markings used on the Varioskan Flash . 4Warning and other markings used in the documentation . 5Instrument safety and guidelines for use. 6Thermo Fisher ScientificChapter 1Introduction to the Varioskan Flash. 15Overview. 15Intended use. 16Advantages of using Varioskan Flash . 16Chapter 2Functional Description . 19Instrument layout. 19Front view. 19Back view . 19Internal view . 20Measurement techniques. 21Fluorescence intensity . 21Time-resolved fluorescence. 21Absorbance. 22Luminescence. 22Optical system. 23Principle of the optical system . 24Excitation optics. 25Measurement optics . 26Emission reading module . 27Photometric measurement module. 28Fluorometric bottom reading . 28Varioskan LumiSens measurement module . 28Dispensers. 29Control switches . 31Incubator . 31Track mechanism. 32Tray options. 33Tray composition. 34Universal tray with adapters . 35Robotic tray . 37How to change the location of the holder for the tip primingvessel . 38How to remove or replace the robotic tray adapter . 39Thermo Scientific Varioskan Flash User Manual7
Contents8Chapter 3Installation.41Installation checklist. 41What to do upon delivery . 42How to unpack . 42Checking delivery for completeness. 42Checking for damage during transport . 42Environmental requirements . 43Things to avoid . 43Technical prerequisites . 43Installation setups. 43How to release the transport locks. 43Tray holder transport lock. 44Varioskan LumiSens optics transport lock . 47How to install the tray . 48How to setup the dispensers . 49Installation of SkanIt Software for Varioskan Flash . 51Cable connections . 51Varioskan LumiSens module installation. 52Varioskan LumiSens measurement position alignment. 56Varioskan LumiSens scaling factor . 58Varioskan LumiSens module filter installation . 58Chapter 4Routine Operation .61Do’s and Don’ts of the Varioskan Flash . 61Do . 61Don’t . 62Switching on . 63Operational check . 64Loading the microplate . 65Automatic runtime calibration . 66Fluorometric measurement. 67Fluorometric spectrum scanning . 70Photometric measurement. 70Photometric spectrum scanning . 71Luminometric measurement . 71Luminometric spectrum scanning . 73Settle delay . 73Other functions. 73Orbital shaking . 73Incubating. 75Dispensing . 76Priming. 78Tip priming . 78Pull-back feature . 79Dispensing and measurement. 79Emptying . 80Dispenser washing. 80Helpful hints. 80Chemical resistance of the dispensers. 81Thermo Scientific Varioskan Flash User ManualThermo Fisher Scientific
ContentsShutdown . 84Emergency situations . 85Thermo Fisher ScientificChapter 5Maintenance. 87Maintenance checklist . 87Regular and preventive maintenance . 88How to clean the measurement chamber. 89How to clean the optics in the measurement chamber. 90How to clean the tray. 91How to clean the reagent basin and dispensing area . 91How to clean the dispensing base . 92How to clean the Varioskan LumiSens optics. 92How to clean the Varioskan LumiSens base . 94Routine maintenance of the dispensers. 96Daily maintenance . 96Weekly maintenance . 96Weak detergent or 10% bleach. 97Weak base and acid in sequence . 97Periodic maintenance . 98If the plunger is stuck. 98Replacing the aspirate tube assembly or the complete dispensingtube assembly . 98Replacing a dispensing tip . 99Replacing a dispenser syringe . 100Replacing the 3-port valve. 101Disposal of materials . 102Decontamination procedure. 102How to refit the transport locks . 104Tray holder transport lock. 104Varioskan LumiSens optics transport lock . 106Maintaining a system log. 107How to pack for service. 107Service contracts. 108Disposal of the instrument . 108Chapter 6Technical Specifications . 111General specifications. 111Performance specifications . 112Safety specifications. 115In conformity with the requirements. 116Chapter 7Frequently Asked Questions. 119Q&As . 119Chapter 8Troubleshooting Guide . 125Error and warning codes . 125Service request protocol. 129Certificate of Decontamination. 129Thermo Scientific Varioskan Flash User Manual9
Contents10Chapter 9Ordering Information .131Varioskan Flash. 131List of spare parts and accessories . 131Upgrade kits. 132Chapter 10References.133Useful web links. 133Literature . 135Fluorescence intensity . 135Time-resolved fluorescence. 135Absorbance. 136Luminescence. 136Appendix ASystem Log.137Appendix BCertificate of Decontamination .139Appendix CThermo Scientific Varioskan Flash Feedback Form .141Appendix DPreparation of Luminometric Alignment Plate.143Required reagents, materials and equipment. 143Product components . 143Reconstitution of assay components . 144Pipetting instructions . 144Thermo Scientific Varioskan Flash User ManualThermo Fisher Scientific
FiguresFiguresFigure 1–1. Varioskan Flash spectral scanning multimode reader. 15Figure 2–2. Varioskan Flash front view. 19Figure 2–3. Varioskan Flash back view . 19Figure 2–4. Close-up of the computer and mains supply connectors . 20Figure 2–5. Varioskan Flash internal views . 20Figure 2–6. Varioskan Flash optics and measurement positions F and L. 24Figure 2–7. Excitation optics . 25Figure 2–8. Principle of the double monochromator. 26Figure 2–9. Measurement optics . 27Figure 2–10. Emission optics. 27Figure 2–11. Photometric measurement module . 28Figure 2–12. Fluorometric bottom reading optics . 28Figure 2–13. Varioskan LumiSens measurement module. 29Figure 2–14. Varioskan Flash dispensing system . 30Figure 2–15. Dispensing tip options: 0.40 mm and 0.25 mm . 31Figure 2–16. Control switches. 31Figure 2–17. Varioskan Flash incubator cross-section . 32Figure 2–18. Part of the Varioskan Flash track mechanism. 33Figure 2–19. Assembly picture of the universal tray . 34Figure 2–20. Robotic tray fitted with adapter for plate w/o lid, #126 . 38Figure 2–21. Changing the location of the tip priming vessel holder . 39Figure 2–22. Removing or replacing the adapter for plate w/o lid, #126 . 40Figure 3–23. Transport locks and transport lock tags present . 44Figure 3–24. Dispenser sliding cover and measurement chamber dooropened. 44Figure 3–25. Front cover removed . 45Figure 3–26. Transport lock fastened (screws 1 – 4 shown). 45Figure 3–27. Releasing the transport lock (screws 1 – 4 shown). 46Figure 3–28. Transport lock in its horizontal storage position (screws 3 and4 shown) . 46Figure 3–29. Varioskan LumiSens optics transport lock fastened. 47Figure 3–30. Varioskan LumiSens optics transport lock removed. 47Figure 3–31. Tray holder. 48Figure 3–32. Fastening the tray frame to the tray holder. 49Figure 3–33. Close-up of the positioning lever when the tray is out . 49Figure 3–34. Varioskan Flash with the dispenser sliding cover open . 50Figure 3–35. Protective cap removed from the dispensing tip (0.40 mm). 51Figure 3–36. Dispenser assembly . 51Figure 3–37. Connecting the mains supply cable . 52Thermo Fisher ScientificThermo Scientific Varioskan Flash User Manual11
FiguresFigure 3–38. Removing the dispensing base.53Figure 3–39. Releasing the green lock knob.53Figure 3–40. Removing the connector protection shield.54Figure 3–41. Connecting the cables .54Figure 3–42. Mounting the Varioskan LumiSens optics.55Figure 3–43. Pulling up the filter lift.59Figure 3–44. Pressing down the filter into the filter wheel .59Figure 4–45. Microplate loaded.65Figure 4–46. Dynamic range selection .68Figure 4–47. Structure of the TRF measurement cycle .69Figure 4–48. ON-OFF period time and total shaking time.74Figure 4–49. Speed & diameter combinations for different plate formats .74Figure 4–50. Identification of the dispensing tip position .76Figure 5–51. Front cover removed .90Figure 5–52. Internal view of the measurement chamber .90Figure 5–53. Opening the dispensing base for cleaning.92Figure 5–54. Removing the Varioskan LumiSens optics .93Figure 5–55. Cleaning the Varioskan LumiSens optics .94Figure 5–56. Cleaning the Varioskan LumiSens base from above.95Figure 5–57. Cleaning the Varioskan LumiSens base from the bottom.95Figure 5–58. Replacing the dispensing tip (A).99Figure 5–59. Replacing the dispensing tip (B) .99Figure 5–60. Dispenser assembly.100Figure 5–61. Replacing the dispenser syringe .101Figure 5–62. 3-port valve replacement.102Figure 5–63. Transport lock released (A) and fastened (B) (screws 1 – 4shown) .105Figure 5–64. Varioskan LumiSens optics transport lock unfastened.106Figure 5–65. Varioskan LumiSens optics transport lock fastened.10612Thermo Scientific Varioskan Flash User ManualThermo Fisher Scientific
TablesTablesTable 2–1. Compatibility of the universal tray and plate-specific adapters 36Table 2–2. Compatibility of the robotic tray and plate-specific adapter. 37Table 3–3. Installation checklist. 41Table 4–4. Front panel indicator light . 63Table 4–5. Readings carried out in measurement positions F and L. 77Table 4–6. Compatibility chart of solvents suitable with the plasticmaterials used in the dispensers. 82Table 5–7. Maintenance checklist . 87Table 5–8. Example of a system log. 107Table 6–9. General specifications . 111Table 6–10. Fluorometry . 112Table 6–11. Photometry. 113Table 6–12. Luminometry . 114Table 6–13. Incubator . 114Table 6–14. Shaker . 115Table 6–15. Dispensers . 115Table 7–16. Plates used with the Varioskan Flash . 120Table 7–17. Tray vs. plate-specific adapter . 120Table 8–18. Error codes reporte
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. This manual supersedes all previous editions. No liability for consequential damages Thermo Fisher Scientific shall not be liable for any
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3V 32M-Bit Serial Flash Memory with Dual and Quad SPI Brief Report Partial Image EN25Q64 EON Flash 64 Megabit Serial Flash Memory with 4Kbyte Uniform Sector Brief Report Partial Image FMND1GXXX3B FIDELIX Flash 3V/1.8V, x8/x16 1G-BIT NAND FLASH Brief Report Partial CA GD25Q64C GigaDevice Flash 3.3V Uniform Sector, Dual and Quad Serial Flash .
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