QUESTION BANK - Jeppiaar Engineering College

JEPPIAAR ENGINEERING COLLEGEB.TECH – BIOTECHNOLOGY (R- 2013)BT6502 BIOPROCESS ENGINEERINGIII YEAR & V SEMBATCH: 2016-2020QUESTION BANKPREPARED BYMs. R. SUGANYA, ASST. PROFESSOR

VISION OF THE INSTITUTION To build Jeppiaar Engineering College as an institution of academic excellence intechnological and management education to become a world class UniversityMISSION OF THE INSTITUTION To excel in teaching and learning, research and innovation by promoting the principlesof scientific analysis and creative thinking. To participate in the production, development and dissemination of knowledge andinteract with national and international communities. To equip students with values, ethics and life skills needed to enrich their lives andenable them to meaningfully contribute to the progress of society. To prepare students for higher studies and lifelong learning, enrich them with thepractical and entrepreneurial skills necessary to excel as future professionals andcontribute to Nation’s economyPROGRAM OUTCOMES (PO)PO 1PO 2PO 3PO 4PO 5PO 6PO 7PO 8Engineering knowledge: Apply the knowledge of mathematics, science,engineering fundamentals, and an engineering specialization to the solution ofcomplex engineering problems.Problem analysis: Identify, formulate, review research literature, and analyzecomplex engineering problems reaching substantiated conclusions using firstprinciples of mathematics, natural sciences, and engineering sciences.Design/development of solutions: Design solutions for complex engineeringproblems and design system components or processes that meet the specifiedneeds with appropriate consideration for the public health and safety, and thecultural, societal, and environmental considerationsConduct investigations of complex problems: Use research-based knowledgeand research methods including design of experiments, analysis andinterpretation of data, and synthesis of the information to provide validconclusions.Modern tool usage: Create, select, and apply appropriate techniques, resources,and modern engineering and IT tools including prediction and modeling to complexengineering activities with an understanding of the limitations.The engineer and society: Apply reasoning informed by the contextualknowledge to assess societal, health, safety, legal and cultural issues and theconsequent responsibilities relevant to the professional engineering practice.Environment and sustainability: Understand the impact of the professionalengineering solutions in societal and environmental contexts, and demonstratethe knowledge of, and need for sustainable development.Ethics: Apply ethical principles and commit to professional ethics andresponsibilities and norms of the engineering practice.

PO 9PO 10PO 11PO 12Individual and team work: Function effectively as an individual, and as amember or leader in diverse teams, and in multidisciplinary settings.Communication: Communicate effectively on complex engineering activitieswith the engineering community and with society at large, such as, being able tocomprehend and write effective reports and design documentation, make effectivepresentations, and give and receive clear instructions.Project management and finance: Demonstrate knowledge and understandingof the engineering and management principles and apply these to one’s ownwork, as a member and leader in a team, to manage projects and inmultidisciplinary environments.Life-long learning: Recognize the need for, and have the preparation and abilityto engage in independent and life-long learning in the broadest context oftechnological change.VISION OF THE DEPARTMENT To pursue excellence in producing bioengineers coupled with research attributesMISSION OF THE DEPARTMENTM1To impart quality education and transform technical knowledge into career opportunitiesM2To establish a bridge between the program and society by fostering technical educationM3To generate societal conscious technocrats towards community developmentM4To facilitate higher studies and research in order to have an effective career /entrepreneurshipPROGRAM EDUCATIONAL OBJECTIVES (PEOS)PEO - 1To impart knowledge and produce competent graduates in the field of biotechnologyPEO - 2To inculcate professional attributes and ability to integrate engineering issues to broadersocial contexts.PEO - 3To connect the program and community by fostering technical education.PEO - 4To provide a wide technical exposure to work in an interdisciplinary environmentPEO - 5To prepare the students to have a professional career and motivation towards highereducation.

PROGRAM SPECIFIC OUTCOMES (PSOs)PSO 1Professional Skills: This programme will provide students with a solid foundation in thefield of Biological Sciences and Chemical engineering enabling them to work on engineeringplatforms and applications in Biotechnology as per the requirement of Industries, andfacilitating the students to pursue higher studies.PSO 2Problem-solving skills: This programme will assist the students to acquire fundamentaland problem solving knowledge on subjects relevant to Biotechnology thereby encouragingthem to understand emerging and advanced concepts in modern biology.PSO 3Successful Career and Entrepreneurship: Graduates of the program will have a strongsuccessful career and entrepreneurial ability with the blend of inputs from basic science,engineering and technology, thereby enabling them to translate the technology and tools invarious industries and/or institutes.

BIO PROCESS ENGINEERING/BT6502OBJECTIVES:To provide the students with the basics of bioreactor engineering.To develop bioengineering skills for the production of biochemical product usingintegrated biochemical processes.UNIT I OPERATIONAL MODES OF BIOREACTORS9Fed batch cultivation, Cell recycle cultivation, Cell recycle cultivation in waste water treatment,two stage cultivation. Packed bed reactor, airlift reactor, fluidized bed reactor and bubble columnreactor.UNIT II BIOREACTOR SCALE – UP8Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors –microbial oxygendemands; methods for the determination of mass transfer coefficients;mass transfer correlations.Scale up criteria for bioreactors based on oxygen transfer, power consumption and impeller tipspeed.UNIT III BIOREACTOR CONSIDERATION IN ENZYME SYSTEMS8Analysis of film and pore diffusion effects on kinetics of immobilized enzymereactions;formulation of dimensionless groups and calculation of effectiveness factors. Design ofimmobilized enzyme reactors – packed bed, fluidized bed and membrane reactors.UNIT IV MODELLING AND SIMULATION OF BIOPROCESSES11Study of structured models for analysis of various bioprocess – compartmental models,models ofcellular energetics and metabolism, single cell models, plasmid replication and plasmid stabilitymodel. Dynamic simulation of batch, fed batch, steady and transient culture metabolism.UNIT V RECOMBINANT CELL CULTIVATION9Different host vector system for recombinant cell cultivation strategies and advantages. E.coli,yeast Pichia pastoris/ Saccharomyces cereviseae, Animal cell cultivation, plant cell cultivation,Insect cell cultivation. High cell density cultivation, process strategies, reactor considerations inthe above system.TOTAL : 45 PERIODSOUTCOMES:Upon completion of Bioprocess Engineering course graduates will be able toSelect appropriate bioreactor configurations and operation modes based upon the nature ofbioproducts and cell lines and other process criteria.

Apply modeling and simulation of bioprocesses so as to reduce costs and to enhance thequality of products and systems. Plan a research career or to work in the biotechnology industrywith strongfoundation about bioreactor design and scale-up.Integrate research lab and Industry; identify problems and seek practical solutions forlarge scale implementation of Biotechnology.TEXT BOOKS:1. Jens Nielson, John Villadsen and Gunnar Liden, “Bioreaction engineering principles”,2ndEdition, Kulwer Academic, 20022. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, IncREFERENCES:1. Anton Moser, “Bioprocess Technology: Kinetics and Reactors”, , Springer Verlag 2011.2. Tapobrate Panda, “Bioreactors: Analysis and Design”, Tata McGraw Hill, 20113. Shijie Liu “Bioprocess Engineering” Elsevier, 20134. Atkinson, B, Mavituna, F, “Biochemical Engineering and Biotechnology Handbook”Macmillan Publishers Ltd, New York, 1992.5. James E. Bailey & David F. Ollis, “Biochemical Engineering Fundamentals”, McGraw Hill.6. James M. Lee, “Biochemical Engineering”, PHI, USA 2002.7. E. Heinzle, A. Biwer and C.Cooney “ Development of Sustainable Bioprocesses” John Wiley& sons, 2006.COURSE OUTCOMES (CO)BT6502: BIOPROCESS ENGINEERINGThe course graduates will be able to Select appropriate bioreactor configurations and operationC302.1 modes based upon the nature of bioproducts and cell lines and other process criteriaC302.2The students will be able to Apply modeling and simulation of bioprocesses so as to reduce costsand to enhance the quality of products and systemsC302.3The students will be able to Plan a research career or to work in the biotechnology industry withstrong foundation about bioreactor design and scale-up.C302.4The students will be able to Integrate research lab and Industry; identify problems and seekpractical solutions for large scale implementation of BiotechnologyThe students will be able to understand the design of plant cultivation reactors, animal cellC302.5 cultivation reactors and recombination techniques.

S. No.1.2.3.4.5.TitleReference BookUNIT I OPERATIONAL MODES OF BIOREACTORS (9)Fed batch cultivationJames E. Bailey & David F. Ollis, “BiochemicalEngineering Fundamentals”, McGraw-Hill.Cell recycle cultivationJames E. Bailey & David F. Ollis, “BiochemicalEngineering Fundamentals”, McGraw-Hill.Cell recycle cultivation inJames E. Bailey & David F. Ollis, “Biochemicalwaste water treatmentEngineering Fundamentals”, McGraw-Hill.Two stage cultivation.James E. Bailey & David F. Ollis, “BiochemicalEngineering Fundamentals”, McGraw-Hill.Packed bed reactorJames E. Bailey & David F. Ollis, “BiochemicalPage No.600-602602-603603-605605-609609-610Engineering Fundamentals”, McGraw-Hill.6.Airlift reactorJames E. Bailey & David F. Ollis, “Biochemical641-645Engineering Fundamentals”, McGraw-Hill.7.Fluidized bed reactorJames E. Bailey & David F. Ollis, “Biochemical614-617Engineering Fundamentals”, McGraw-Hill.8.Bubble column reactor.James E. Bailey & David F. Ollis, “Biochemical610-614Engineering Fundamentals”, McGraw-Hill.9.10.11.UNIT II BIOREACTOR SCALE – UP(8)Regime analysis of bioreactorJames M. Lee, “Biochemical Engineering”, PHI, USA.processesOxygen mass transfer inJames M. Lee, “Biochemical Engineering”, PHI, USA.bioreactorsMicrobial oxygen demands;James M. Lee, “Biochemical Engineering”, PHI, USA.240-247241-242261-262James M. Lee, “Biochemical Engineering”, PHI, USA.264-265James M. Lee, “Biochemical Engineering”, PHI, USA.248-25014. Scale up criteria for bioreactorsJames M. Lee, “Biochemical Engineering”, PHI, USA.based on oxygen transfer,power consumption andimpeller tip speed.UNIT III BIOREACTOR CONSIDERATION IN ENZYME SYSTEMS (8)15.Analysis of film and poreJames M. Lee, “Biochemical Engineering”, PHI, USA.diffusion effects on kinetics ofimmobilized enzymereactions;272-27412.13.16.Methods for the determinationof mass transfer coefficients;Mass transfer correlations.Formulation of dimensionlessgroups and calculation ofeffectiveness factors.17. Design of immobilized enzymereactors – packed bedHarvey W. Blanch, Douglas S. Clark, “BiochemicalEngineering”, Marcel Decker Inc.James E. Bailey & David F. Ollis, “Biochemical202,216208128,122609-611