FROM SMALL MOLECULES TO LIVE CELLS THE ASSAY

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FROM SMALL MOLECULES TO LIVE CELLSTHE ASSAY THATMEASURES IT ALLAlpha Technology Solutions

MANYAPPLICATIONSToday’s researchers study a widevariety of targets, from largeendogenous protein complexesto very small molecules, andeverything in between. Samplescan range from serum andplasma to crude cell lysatesand purified reagents. So whenyou need an efficient, highlyadaptable assay to study a variety of targets, there’s only onesolution: Alpha Technology. Alpha (amplified luminescent proximityhomogeneous assay) is a versatile, bead-based platform that givesyou the power to assay even the most complex samples – all in onewell, with no wash steps.ONE PROVENASSAYOur Alpha Technology is part of a complete solution that includes acomprehensive assay platform, Alpha-certified detection, automationinstrumentation, and custom assay development. It’s everything youneed to ensure consistent, reproducible results across a wide varietyof applications.2

ALPHA TECHNOLOGY SOLUTIONSChoose the Alpha solution that’s right for youAlpha Technology is a bead-based proximity assay. When Alpha donor and acceptor beads arebrought together, a cascade of chemical reactions is set in motion, creating a greatly amplified signal.The highly versatile beads can be coated with various biomolecules, enabling detection of uniquebiological events. There’s an Alpha Technology solution for just about every research requirement:Alpha Donor BeadAlpha Acceptor BeadAlphaLISA For results inAlphaScreen SureFire AlphaScreen Perfect forhalf the time of ELISA, withoutwash steps, and with excellentsensitivity in a variety of matrices,including serum and plasmaGreat for cellular kinase assays,allowing detection of endogenousphosphoproteins in cell lysatesand disease-related signaltransduction pathwaysstudying fusion-tagged proteinsor protein:protein interactions,and for testing cAMP, cGMP,phosphorylated peptides, orsamples in a simple matrixOn PointGST-taggedHDM2reagent servicesBiotinylatedP53Streptavidin-coatedAlpha Donor BeadAnti-GST-conjugatedAlphaLISA Acceptor BeadAlpha Toolbox Enables youOnPoint SM Reagent ServicesMultimode Readersto set up your own assay usingready-to-use Alpha beads fornumerous capture solutionsDelivering custom-built kits toyour exact specificationsAlpha-certified EnVision andEnSpire Multimode PlateReaders, enabling you to deliveroptimal results, time after timeAlpha Technology. It’s the simple, no-wash assay that puts you in close proximity – for better results sooner.How Alpha Technology Assays WorkThe simple, no-wash assay that allows for easy adoption, decreased variability, and results in half the time of a traditional ELISA.Add analyte tomicroplate.Add biotinylatedanti-analyte antibodyand anti-analyteantibody-conjugatedacceptor beads.Incubate for 60 minutes.Add streptavidin-coateddonor beads. Incubatefor 30 to 60 minutes. Theinteraction between beadbound molecules andanalyte brings the beadsinto ha-reagentsExcite the donor beadswith red light.Singlet oxygen generatedby donor beads excitesthe acceptor beads, whichemit light proportional tothe level of interaction.3

IT'S SIMPLECONSISTENTREPRODUCIBLEAND RIGHT ON TARGETSince the introduction of genetically engineered human insulin in the 1970s, biotherapeutic drugs have beenapproved for treatment of many diseases, including cancer, inflammatory disease, and rare diseases withhighly targeted and specific natures. As biotherapeutics is a highly regulated arena, researchers are looking tonovel techniques that will allow them to develop these drugs faster, while still meeting regulatory demands.That’s the idea behind our consistent, efficient Alpha Technology.TARGETIDENTIFICATIONCLONING &EXPRESSIONCELL CULTUREPROCESSDEVELOPMENTMANUFACTURING& QCTarget identificationCloning and expressionAlpha Technology provides results in half the time ofELISA, when looking at cell response to a stimulus.Alpha Technology is gaining in popularity amongresearchers because it requires no separation orwashing and runs in half the time of ELISA.To help you validate your targets, we offer specializedreagents for almost any biological event. cAMP, cGMP Phosphorylation Cytokine secretionThe technology simplifies your workflow andimproves data quality generated during the cloningand expression process. Clone screening and selection Isotyping Phage panning Functional screeningAlpha Technology: The Clear DifferenceCompared to standard assays, AlphaLISA delivers benefits that are easy to measure: Consistent results (%CVs less than 10%) Results in half the time of ELISA Superior sensitivity, exceeding FDA guidelines of 250-500 ng/mL, without wash steps Easy, automation-friendly protocol4SAFETY &PRE-CLINICAL

BIOTHERAPEUTICSAlpha TechnologyADD ACCEPTORBEADS &BIOTINYLATEDANTI-ANALYTEADD ANALYTE& INCUBATE30-60 MINSADD DONORBEADS &INCUBATE30-60 MINSREADStandard Immunoassay(including heterogeneous electrochemiluminescence assay)ADD IgGCAPTURE& INCUBATE4 HOURSADDBLOCKINGSOLUTION &INCUBATE1 HOURWASH UPTO 3 TIMESADD SAMPLE& ANTIBODYDETECTIONSOLUTIONBLOCK &INCUBATE1 HOURWASH UPTO 3 TIMESADDDETECTIONIgG &INCUBATE2 HOURSWASH UPTO 3 TIMESADD READBUFFER ORSUBSTRATEProcess developmentSafety and preclinicalIdentifying process-related impurities ensures thequality and safety of biotherapeutics and vaccines.Highly sensitive, robust Alpha kits are developed andmanufactured to satisfy strict performance requirementsfor the detection of key drug impurities. The simple, fastprotocol provides accurate results while allowingfor efficient transfer between departments.Safety of biotherapeutics – and ultimately, patients –depends on high-quality, reproducible data. Fucosylated IgG quantitation Cell culture additives Antibody glycosylation Host cell protein detection Residual Protein A Lot release assaysREADWhether your work involves monoclonal antibodies orproteins and peptides, the robust inter- and intra-assayconsistency, optimal detection of true positive samples,and simple protocol of Alpha Technology streamlineyour workflow and provide an ideal alternative to ELISAor ECL assays. Antidrug antibody (ADA) assays Neutralizing antibody (NAb) assays Pharmacokinetics (PK) Pharmacodynamics (PD)Manufacturing and QCYou can save precious time and avoid bottlenecks in manufacturing and QC processes using AlphaLISA assays.AlphaLISA reduces hands-on time and automation stress while improving reproducibility, delivering minimal lot-to-lotvariation between reagents and improved confidence in your crucial QC and lot-release assays. More than 120 biomarker detection kits Functional assays Process-contaminant detection and lpha-reagents5

IDENTIFYEPIGENETICDRUG CANDIDATESFASTEREpigenetics is a rapidly growing field that presents a significant opportunity for drug discovery. So thedevelopment of robust and biologically relevant assays for screening, lead identification, and optimizationis more critical than ever before.We deliver the first portfolio of epigenetics assays fully validated for mark specificity and optimized for highthroughput applications, while providing the flexibility to work across the full spectrum of substrates – frompeptides, histones, and nucleosomes to truly nonrecombinant cell lines. These screen-ready assays accelerateepigenetic drug discovery by improving the efficiency of lead-compound identification and enablinginterrogation of hit validity in more biologically relevant contexts.AlphaLISA for Peptide ModificationAlphaLISA for Full-length Protein ModificationsKBK EnzymeSAM EnzymeSAHSAMSAHMeKBMeK Eu-Ab ULight-SA SA-Donor Bead Biotinylated Ab Ab-Acceptor BeadFRETEuEm 665 nmMeULightBEmission615 nmExcitation320 or 340 nmKMeKExcitation680 nmBPerkinElmer epigenetics assays are available in two homogeneous formats: AlphaLISA detection assays for peptide and full-lengthprotein modifications. Let your science determine your screening strategy.6

EPIGENETICSBiochemical ToolboxThe Epigenetic Biochemical Toolbox reagents are available forboth AlphaLISA and LANCE Ultra TR-FRET platforms, highlysensitive and robust technologies that offer simple, no-washprotocols that are automation-friendly. All reagents havebeen tested extensively to ensure they detect the specificepigenetic mark of interest with minimal cross-reactivity.Assays are also optimized to deliver the highest sensitivity,even at low enzyme concentrations, and are completely HTScompatible. The toolbox provides the flexibility to work acrossthe full range of substrates, from peptides to histones towhole nucleosomes – an enabling feature when working withchallenging enzymes that exhibit substrate preferences and/orlow turnover.Cellular Detection KitsComplementing the Biochemical Toolbox are the novelAlphaLISA Epigenetic Cellular Detection Kits for cell-baseddetection of epigenetic modifications. These are the firstcellular assays on the market that offer the utmost sensitivityA Growing Portfolio of ReagentsAssays for key epigenetic enzymes, including: DOT1L EZH2 G9a HDAC1 LSD1 JMJD2A NSD2 PRMT1 SET7 TIP60For the most up-to-date portfolio information,visit eagentsand enable rapid and direct detection of endogenousmodification of epigenetic marks in a one-well, no-washassay. The simple, user-friendly protocol eliminates theneed for tedious ELISA and western blot assays. The kitsare suitable for endogenous and recombinant cell lines,providing the flexibility to work with biologically relevantcell models.ALL-IN-ONEWELL KITS:PROTOCOLCELLULARDETECTIONALL-IN-ONE WELL PROTOCOLPREPARATIONOvernightEXTRACTION25 minDETECTION90 minRead Plateon EnVision or EnSpire Seed cells in plate4 hoursAdd Cell-HistoneLysis Buffer15 minAdd Acceptor Beads andBiotinylated Anti-H360 minTreat cells –overnightAdd Cell-HistoneExtraction Buffer10 minAdd Donor Beads30 ents7

THEELISAALTERNATIVE FORBIOMARKERSWITH NO LIMITSImmunoassay technology continues to be a critical part of how we understand concentrations of biologicalmaterial in various sample media. ELISA and radioimmunoassays (RIA) have been the industry standard formany years but are known to be labor intensive and don’t always provide the reliability scientists require intoday's competitive environment. AlphaLISA no-wash immunoassays, as well as our complementary DELFIA and LANCE assay platforms, ensure that your immunoassays perform as sensitively, efficiently, and accuratelyas your research requires – in half the time of ELISA. For optimum results, use EnSpire and EnVision platereaders, which have been validated for use with Alpha, DELFIA, and LANCE technologies.A highly sensitive, homogeneous alternative to traditional ELISA technology, the AlphaLISA assay platformdelivers a wide dynamic range and easy automation for analysis of biomarkers and other analytes.AutophagyCentral Nervous SystemOur exclusive AlphaLISA platform offers results inhalf the time of ELISA with superior sensitivity anddynamic range for analyzing key autophagy targets,including LC3B and p62.CNS research faces the same hurdles as othertherapeutic areas – the need to move faster withlimited resources. Traditional methods fall short inmeeting these requirements. Save time and decreasecosts using AlphaLISA kits developed specifically forthe most important CNS markers, including Tau,amyloid β, and APP.Biologics/BioprocessAlphaLISA kits shorten the time it takes to quantitatemonoclonal antibodies and detect contaminants,impurities, host cell proteins, cell culture additives,and leachables to less than three hours while stillimproving %CVs.CancerMeasure more cancer biomarkers faster with a highlyreproducible ELISA alternative. Choose from a broadrange of AlphaLISA kits for key targets that utilize asimple protocol and small sample volumes (1-5 μl).CardiovascularAchieve results in half the time of ELISA and conserveprecious samples so multiple cardiovascular markerscan be run faster, significantly decreasing hands-ontime and costs with AlphaLISA.InflammationAlphaLISA allows the measurement of keybiomarkers and cytokines in assays developedspecifically for your targets. Elimination of transferor wash steps shortens the run time and improvesreproducibility, while maintaining wide dynamicrange and high sensitivity.MetabolicWith AlphaLISA you can shorten the time it takesto measure multiple biomarkers such as insulin,C-peptide, leptin, and FGF21, while conservingprecious samples.VirologyThe AlphaLISA HIV p24 research immunoassay kit isdesigned for the quantitative determination of HIVp24 in buffered solution or cell culture medium.8

BIOMARKERSAlphaLISA Kits for Biomarkers and 2LC3BBiologics/BioprocessAlbumin (human)CHO-PCHO-P broad reactivityIgAIgEIgGIgG1 (isotyping)IgG2 (isotyping)IgG3 (isotyping)IgG4 (pharmacokinetic)IgMNSO-PResidual Protein AE. coli HCPIgG1 (pharmacokinetic)IgG4 (isotyping)PER.C6CancerAFPEPOMMP1MMP9 (mouse)TFF3CA125Caspase-3 CL11/I-TACIFN-ßMMP9TNFαCardiovascularAlpha-2 macroglobulinICAM-1PCSK9Cardiac Troponin roreninEPOPAI-1tPACentral Nervous SystemAmyloid ß 1-15Amyloid ß 1-42Amyloid ß oligomersAmyloid ß 1-40Amyloid ß 1-42 (high specificity)sAPPα (C-term specific)Amyloid ß 1-40 (high specificity)Amyloid ß 1-42 (mouse/rat)TauAmyloid ß 1-40 (mouse/rat)Amyloid ß -CSF (mouse)IL1αIL2 (mouse)IL6IL8IL1ß (rat)IL15 (mouse)TNFαCCL2/MCP1CCL2/MCP1 (mouse/rat)CCL5 / RANTESCCL5 / RANTES (mouse)CXCL10/IP-10G-CSFIFN-αIFN-ΥIL1ßIL1ß (mouse)IL3IL4IL6 (mouse)IL7IL10IL10 (mouse)IL12 (p70)IL13IL17IL17A (mouse/rat)TNFα (mouse)CCL3/MIP-1αCRPGM-CSFIFN-Υ (mouse)IL2IL5IL7 (mouse)IL11IL15IL18MetabolicAdiponectinC-peptide (mouse/rat)IGF1Leptin (mouse)Adiponectin (mouse)Albumin (human)FGF21GLP-1IGF2InsulinProlactinAlbumin (mouse)Growth hormoneLeptinVirologyHIV p24 (high sensitivity)HIV p24TNFα (mouse)VEGFDVEGFA (mouse)VEGFWe are regularly adding new kits, so for the most up-to-date information, visit /www.perkinelmer.com/category/alpha-reagents9

HANDLE LARGEPROTEIN:PROTEININTERACTIONSWITH EASEProtein:protein interactions are implicated in a large number of biological processes. With Alpha Technology,you benefit from a remarkably efficient and sensitive assay, enabling you to study the interactions andcomplexities of protein epitopes interacting within 200 nm distance.A highly flexible, homogeneous, bead-based proximity assay, Alpha Technology offers the possibility to assaymany biological targets, including enzymes, receptors, ligands, second messengers, DNA, RNA, proteins,peptides, sugars, and small molecules and their corresponding interaction partners. The range of interactionaffinities measurable with Alpha is huge – even low affinity interactions like sugar/lectin can be measured.Get the Alpha Technology advantageChoose Alpha Technology for the study of proteins and theirinteractions using both biochemical and cell-based assays.GST-taggedHDM2AlphaLISA – Delivers the greatest flexibility with a toolboxrange of beads to mix and a Donor BeadAnti-GST-conjugatedAlphaLISA Acceptor BeadAlphaScreen – Best off-the-shelf solution, ideal for fusiontag detectionBinding of GST tag p53 to HDM2.Alpha Technology offers: i2{Ni 2 {N{Ni2 {Ni2 {Ni 2 Ability to detect interactionsinMEK1crude cell extracts or withHis-taggedAnti-pTpY ERK1/2PSPS nM and biologically relevantpurified proteins used atMEK1lowEmissionPT572 nmPYERK2concentrationsEmission520-620 nm680 nmi 2 {N{Ni2 i2{N{Ni2 {Ni 2 AlphaScreen Bead No separation steps; homogeneous no-wash assay{Ni2 {Ni2 Streptavidin-coatedAlpha Donor Bead{Ni 2 {N i2Alpha Donor Bead Flexibility – choosecustom or nylatedE6AP Peptide i2{GSH-coated{Ni2 Emission615 jugatedAlphaScreen Acceptor BeadAnti-GST-conjugatedHis-tagged MEK1AlphaScreen Acceptor BeadAnti-pTpY ERK1/2PSPSEmissionMEK1PT572 nmPYERK2GST-tagged ERK2sion20 nmExcitation680 nmGSH-coatedAlpha Donor BeadAnti-mouse-conjugatedAlphaScreen BeadonjugatedAcceptor Bead10GST-tagged ERK2 Cost-efficiency:use small amounts of samples and reagentsExcitation Easy miniaturizationNickel Chelate-coatedAlphaLISA Bead GST-tagged E6n i2{Ni2 Excitation680 nmEmission520-620 nmi 2 AlphaScreen with recombinant protein and peptide,Hsp90 with its cochaperone HOP.sion20 nm{N i2{NNickel Chelate-coatedAlphaScreen Acceptor Bead{Ni2 {Ni2 Ability to detect weak interactions that would be difficult toChelate-coateddetect usingNickeltraditionalassays; this allows highly sensitiveAlphaLISA BeadEmissionmeasurementof low-affinity and high-affinity interactions (low615 nmmillimolar to picomolar range){Ni 2 Streptavidin-coatedAlpha Donor Beadrimeric Complex-conjugatedphaScreen Acceptor Bead{N{Ni2 i2HOPBiotinylatedHsp90 Peptide{NHis-taggedHOP TPR2A{Ni2 Emission520-620 nm{Ni2 Excitation680 nmEmission520-620 nmAlphaScreen and AlphaLISA in a single well withrecombinant proteins. EnVision and EnSpire systems for studying interactions andcomplexities of proteins up to 200 nm in size.

PROTEIN:PROTEINCustom or off-the-shelf technologies – you decideAntibody CaptureFusion Tag DetectionThe Alpha Toolbox gives you a wide range of beads coated with commonly used antiprotein tag antibodies and binding motifs,allowing you to build an assay to measure complex biological interactions. We also provide unconjugated beads that you can coatyourself. It’s up to you – the possibilities are endless.Alpha Donor BeadsAlphaLISA Acceptor BeadsStreptavidinStrep Tactin Nickel ChelateGlutathione (GSH)Anti-FLAG StreptavidinStreptavidinStrepTactinTactin StrepNickelChelateNickel ChelateGlutathione(GSH)(GSH)GlutathioneAnti-FLAG x HisAnti-V5Anti-GFPAnti-Maltose-BindingProtein (MBP)LCA (Lens CulinarisAgglutinin)Alpha Donor BeadsAlphaLISA Acceptor BeadsProtein AAnti-rabbit IgG†Anti-mouse IgG†Protein AProtein GProtein LAnti-human IgG†Anti-human IgG1(pharmacokinetic)Anti-human IgG4(pharmacokinetic)Anti-human IgG1 (isotyping)Anti-human IgG2 (isotyping)Custom coatings and conjugations are available.†Fc-specific.*AlphaScreen acceptor beads emits 520-620 nm.**Kits include both a donor and acceptor bead.AlphaScreenAcceptor BeadsAlphaScreen Fusion TagDetection Kits**Nickel c-MycDIGStreptavidinFITCStrep FLAGTactin Chelate)Anti-FLAG Biotinylated-GSTBiotinylated-HISAlphaScreen Acceptor BeadsAnti-human IgG3 (isotyping)Anti-human IgG4 (isotyping)Anti-rabbit IgG†Anti-mouse IgG†Anti-rat IgG†Anti-goat IgG†Anti-sheep IgG†Anti-mouse IgMAnti-chicken IgYProtein AAnti-rabbit IgG†Anti-mouse IgG†Control BeadsOmnibeadsTruHits KitWe are regularly adding new kits, so for the most up-to-dateinformation, visit /www.perkinelmer.com/category/alpha-reagents11

KINASESKINASE ASSAYSTHAT DELIVERTHE POWER OFCHOICEKinases are an important target class in drug development, not only due to their importance in diseaseetiology, but also because they are considered to be highly “druggable” – in other words, they’re a targetthat can be chemically modulated by small molecules (cytosolic kinases) or larger biologics (receptortyrosine kinases).Alpha Technology is ideal for developing nonradioactive, homogeneous assays for the direct measurement ofphosphorylated peptides and full-length protein substrates – with no wash steps required – in a biochemicaland cellular format.Biochemical assaysExcitation680 nmEmission615 nmBiotin-ProductPrimary AntibodyBoth AlphaScreen and AlphaLISA technologies can be used forbiochemical assays, but AlphaLISA is an ideal solution for whenyou need to work with complex matrices such as cell lysates.It's compatible with substrates such as lipids, peptides, and fulllength proteins, and provides a simple protocol for performingtyrosine, serine/threonine, and lipid kinase assays.Cellular assaysEmission520-620 nmExcxciitatattionExcitationnm680 nmPhospho-substrateStreptavidin-coatedAlpha Donor BeadProtein A ConjugatedAlphaScreen Acceptor BeadCytokineReceptorsGPCRsGrowth nalingSPTyrosineKinaseReceptorSTAT1,, STAT3,STSTATTATAT33, STAT5STStress/InflammationSignalingALK, C-MMETET, EGEGFGF, ErbB2,C-MET,EGF,ErbB2,IGF-1, Insulin, VEGFIκB,B IKKIKK,KK, NNFNFF-κBB,,SMAD1,SMAD2, SMAD3AKT/mTORSignaling4E-BP1BP1P11, AAKTKT,KTT BABBADAD,4E-BP1,AKT,BAD,Caspase 9, GSK-3, mTOR,p70S6K, PDK-1, S6 RPp70S6KOtherTargetsbetacaterin,betacateterinrini , ChChk1,Chkhk , Chk2,hk1Ccofilin, CREB, eIF2a, GAPDH,Histone H3, p53, Topo IIAMAPKSignalingATF1, ATFTFF2, c-Jun,c Junc-Juc-Jun c-Raf,un,ATF2,elF4E, Elk-1, ERK,7 JNK, MEK, MKK3/6,HSP27,MKK4, p38 MAPKAlphaScreen SureFire signaling targets.12When testing the response of endogenous phosphorylatedprotein receptors, AlphaScreen SureFire delivers results – withrobust S/B ratios to Z1 values. Established cell lines as wellas primary and stem cells can be used, and you can rely onendogenous or overexpressed targets as desired.As the assay can be run in just a few hours, you move quicklyfrom cell lysis to quantitative results. It's a nice, homogeneousalternative to western blots that allows you to use fewer cellswhile getting more replicates of each condition tested.

GPCRsTHE SIMPLE WAYTO MEASURECOMPLEX GPCRsYou need versatile and reliable assays to study multiple signaling pathways activated by GPCRs. So we providea variety of high-performance Alpha Technology solutions for you to choose from, enabling you to measurecAMP levels or endogenous levels of phosphorylated MEK, ERK, AKT, and CREB, quickly and easily. What’smore, the EnSpire Multimode Plate Reader with Alpha and Corning Epic label-free technology allows you tostudy subconfluent cells and difficult GPCR targets using two orthogonal approaches – labeled and label-freeanalyses on one versatile platform.Emission520-620 nmExcitation680 MPRTKGbGaiBiotinylBiotinylated-cAMPGasAnti-cAMP ConjugatedAlphaScreen Acceptor BeadDetection of cAMP with AlphaScreen is based onthe competition between cAMP produced by cellsand a biotinylated cAMP probe that’s recognized bythe streptavidin-donor and anti-cAMP conjugatedacceptor beads. AlphaScreen cAMP provides veryhigh sensitivity for measuring Gi- and Gs-coupledGPCRs and delivers very large signal-to-backgroundratios and high Z1 values, in a technology that’sextremely simple to automate.mSoscAMPIP3PKAPKCCAlphaScreen qAdenylateCyclaseStreptavidin-coatedAlpha Donor BeadG?Shcb-Arrestinb-ArSrcCA2 CaMKIICaMKKIIRapRaRaffRPI3KMEK 1MPPIP3PDKImTorERK 1/2AKTAlphaScreen SureFire MEK, ERK, AKT, andCREB assaysTo measure endogenous levels of phosphorylated ERKwith the highest levels of sensitivity, you can rely onour AlphaScreen SureFire technology. It allows youto screen tough GPCR targets not optimally coupledthrough cAMP or calcium pathways. Plus, it enables youto evaluate secondary pathway activation/inhibition in ahigh-throughput format.Expand your potential with orthogonal analysisEnSpire Multimode Plate Reader withCorning Epic label-free pha-reagentsThe EnSpire Multimode Plate Reader delivers establishedCorning Epic label-free technology in addition to Alphalabeled technology. Multimode detection means you cancharacterize GPCRs in all classes, taking a complementary,confirmatory approach that enables you to describemultiple receptor subtypes and/or modes of action.13

WE CARE ABOUTYOUR APPLICATIONYOUR CONSUMABLESYOUR SCIENCEAt PerkinElmer, we share your commitment to answering challenging biological questions. Our broadportfolio of instruments, reagents, assays, informatics, and services empowers you to do more. You’llhave the flexible, efficient solutions you need to study a large number of biological processes andinteractions. No matter where your research stands, we can help you move it forward with the dataand analysis that lead to insight – and breakthrough answers.Ask an expert about your applicationWant to create your own assay? Scientist to scientist, our team of fieldapplication experts is here to give you the in-lab support, guidance, andexpertise to accelerate your science and drive your business.Assay Support Knowledge Base (ASK)Find concise technical overviews, help with assays, video tutorials, and practicaltips and troubleshooting – all intended to help you use PerkinElmer reagentsfor a wide range of applications. More at www.perkinelmer.com/ASKPerkinElmer Life Sciences Citations LibraryExplore hundreds of useful citations and peer-reviewed scientific literaturepublished by scientists who use our products. Search by abstract, key words,journal title, or author at www.perkinelmer.com/citationsPerkinElmer MicroplatesWhy trust your precious samples to generic labware plates when you canhave the microplates that are exclusively certified for your Alpha assay? Allour microplates have been designed to optimize your assay results with lowbackground, optimum light transmission, and little or no crosstalk. And theymeet SBS/ANSI standards.Choose the plates that best fit the research you do, including: AlphaPlates OptiPlates ProxiPlates Half-area plates14AlphaPlates are specially designedto optimize your Alpha assay results,reducing crosstalk that may result fromdifferences in sample concentrationson the same plate.

SERVICE AND SUPPORTOnPoint Custom and Reagent ServicesYour scientists are your most valuable resource. But when they haveto develop their own assays and reagents, they have less time tospend investigating and innovating. That's the thinking behind ourOnPoint Custom and Reagent Services – the complete solution foraccelerating and simplifying life science research and drug discovery.We know that no two projects are exactly alike. If you're workingon a proprietary target or don't find what you need in our catalog,we can design a customized solution for you. OnPoint scientistsare experts in our state-of-the-art assay and reagent technologiesand detection instrumentation, so they’re able to recommendsolutions from one of the industry's broadest portfolio ofinnovative technologies – the platform of choice for many leadingbiotechnology and pharmaceutical companies around the world.These solutions include: Assay development for biotherapeutics, epigenetics,protein:protein interactions, protein:DNA interactions,protein:RNA interactions, biomarker, GPCR, kinase, phosphatase,or ELISA conversion assays Custom labeling services Custom microplate barcoding and coatingOn Pointreagent servicesThe OnPoint Assay Development ProcessOnPoint Scientific ConsultationOur Assay Development Scientists conduct a technical assessment,discussing assay and reagent specifics and performance criteria.Assay and Reagent Development ProposalThe OnPoint team creates a customized quote and serviceproposal for your project, including a detailed description of theassay development plan, deliverables and estimated timelines.Development and OptimizationThe team provides preliminary feedback on progress and reportskey milestones and any suggested changes or additions to theproject plan, which are only implemented with your approval.Complete Technology TransferThe transfer process includes: Proof-of-concept report Training on your assay, in your lab by one of our expertDrug Discovery Application Specialists Custom radiosynthesisWe can also give you advice and support, enabling you to developthe assay yourself, in your own lab.New ways to integrate and visualize your dataMake new discoveries by combining data from different sources and looking at them from different angles. TIBCO Spotfire software is the collaborative platform for data aggregation and visualization. It makes it easy for you to integrate data acquiredfrom experiments using Alpha and other detection technologies, such as luminescence, absorbance, fluorescence, and label-free,with compound data, such as structures and chemical properties, enabling you to create intuitive visual dashboards to analyzecomplex data in minutes. With a few clicks, you’ll be running visualizations and making new discoveries.To accelerate your biological discovery processes even further, our E-Notebook for Biology solution delivers an intuitive, flexible,collaborative electronic lab notebook that minimizes the time you spend collecting, collating, analyzing, and managing data,giving you more time to concentrate on your -reagents15

PerkinElmer, Inc.940 Winter StreetWaltham, MA 02451 USAP: (800) 762-4000 or( 1) 203-925-4602www.perkinelmer.comFor a complete listing of our global offices, visit www.perkinelmer.com/ContactUsCopyright 2013-2021, PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.Corning and Epic are registered trademarks of Corning Incorporated. AlphaLISA and AlphaScreen reagents are for research use only.438473 (011097 01) PKI

interaction between bead-bound molecules and analyte brings the beads into proximity. Excite the donor beads with red light. Singlet oxygen generated by donor beads excites the acceptor beads, which emit light proportional to the level of interaction. ALPHA TECHNOLOGY SOLUTIONS AlphaScreen Per

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