2019 Annual Meeting Midwestern Section American Society Of .

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2019 Annual MeetingMidwestern SectionAmerican Society of Plant BiologistsMarch 16 – 17, 2019South Agricultural Sciences Buildingand Agricultural Sciences BuildingWest Virginia UniversityMorgantown, WV

Thanks to our Sponsors!United States Department of AgricultureNational Institute of Food and AgricultureThis conference is supported by the Agriculture and Food Initiative (AFRI) [award no. 2019-6701429243/project accession no. 1018778] from the USDA National Institute of Food and Agriculture.

American Society of Plant Biologists Midwestern SectionSection Officers 2018 – 2019ChairKathrin Schrick (kschrick@ksu.edu) Kansas State UniversityVice ChairHarkamal Walia (hwalia2@unl.edu)University of Nebraska-LincolnSecretary-TreasurerSen Subramanian (senthil.subramanian@sdstate.edu)South Dakota State UniversityMeeting OrganizerMichael Gutensohn (michael.gutensohn@mail.wvu.edu)West Virginia UniversityExecutive Com RepGustavo MacIntosh (gustavo@iastate.edu) Iowa State UniversityPast Chair, ex officioDavid Rosenthal (rosentha@ohio.edu) Ohio UniversityPublications ManagerJennifer Robison (jenrobis@umail.iu.edu )Indiana University Purdue University IndianapolisShort ProgramFriday, March 152:00 – 4:00 . Guided Tour of the WVU Evansdale Greenhouse and the InternationalCulture Collection of (Vesicular) Arbuscular Mycorrhizal Fungi (INVAM)(Meet in Greenhouse Lobby)2:00 – 4:00 .Guided Tour of the WVU Arboretum (Meet at Coliseum Parking Lot)Saturday, March 167:00 – 8:00 .Registration/Check-in/Poster Set-up/Breakfast8:00 – 8:15 .Welcoming Remarks8:15 – 8:45 . Featured Speaker Dr. Gregg Howe8:45 – 10:15 .Oral Session I (T1-T6)10:15 – 11:30 . Poster Session I (Even #s)/Refreshments11:30 – 1:00 . Oral Session II (T7-T12)1:00 – 2:30 . Lunch1:30 – 2:30 .Career Panel2:30 – 3:00 . Featured Speaker Dr. Steve DiFazio3:00 – 4:30 . Oral Session III (T13-T18)4:30 – 4:45 . Coffee Break

4:45 – 5:45 . Keynote Speaker Dr. Elizabeth Haswell5:45 – 7:15 . Poster Session II (Odd #s) / Appetizers7:30 – 10:00 . Banquet DinnerSunday, March 177:00 – 8:00 . Registration/Check-in/Breakfast8:00 – 9:30 . Oral Session IV (T19-T24)9:30 – 10:00 . Morning Refreshments/ Remove Posters10:00 – 10:30 . Featured Speaker Dr. Jean-Michel Ané10:30 – 12:00 . Oral Session V (T25-T30)12:00 – 12:30 . Business Meeting, Announcements, AwardsMeeting LocationsRegistration .South Ag Sciences BldgAll Presentations including Keynote Address .James H. Arbuckle Lecture HallSaturday Career Panel Discussion .Lecture Hall G006Poster Viewing .Agricultural Sciences BldgSaturday and Sunday Breakfast . South Ag Sciences BldgSaturday Lunch. Agricultural Sciences BldgSaturday Coffee Break .South Ag Sciences BldgSaturday Dinner. Evansdale Crossing BldgVegetarian options will be available as part of all meals provided; however, preference will be given toindividuals who requested vegetarian meals during meeting registration.Posters: Posters need to be mounted on poster boards between 7:00 am and 9:30 am on Saturday, March 16.Due to space limitations, poster dimensions should be NO larger than 36 in. (width) by 42 in. (height). Pins willbe available on-site. Posters need to be removed from poster boards on Sunday, March 17, between 9:30 am and1 pm. Items remaining on display after 1 pm on Sunday will be discarded.Photographing, videotaping, or recording of any kind is prohibited (including but not limited to cameraphones and digital devices).Notice to Oral Presenters: The preferred format for presentations is PowerPoint. In order to expedite sessionsand remain on schedule, we ask you, if possible, to email your presentations to Michael Gutensohn(michael.gutensohn@mail.wvu.edu) by 5 pm on Thursday, March 15th. Alternatively, you may bring a copy ofthe presentation with you on a USB Flashdrive (labeled with your name) to present at the registration check-indesk. Please name your presentation file with your name, the date you are speaking and the presentation numberas listed in the meeting program. For example: Barbara Smith March 16 Talk 10. This will assist us further inmaking sure your presentation is uploaded to the correct session on the lecture hall computer. We ask all oralpresenters to arrive 10 minutes prior to the start of their session to ensure their presentations are loaded andready. The presentation room will have a computer, projector, screen, remote control and laser pointer for youruse.

2019 Annual MW ASPB Meeting, West Virginia UniversityFriday, March 152:00 – 4:00Guided Tour of the WVU Evansdale Greenhouse and theInternational Culture Collection of (Vesicular) Arbuscular Mycorrhizal Fungi(INVAM) Meet in the Greenhouse LobbyThe tour will include a visit to the new 18,615 square-foot Evansdale Greenhouse built in /centers-initiatives/wvu-evansdale-greenhouse). The 16bay greenhouse is conveniently located next to the Agricultural Sciences Building. The tour will alsoinclude an introduction to the INVAM (https://invam.wvu.edu/), the world’s largest collection ofvesicular-arbuscular mycorrhizal fungi. Parking will be available in lot 43 across from the main entranceto the greenhouse.2:00 – 4:00Guided Tour of the WVU Arboretum . .Meet at Coliseum Parking LotThe WVU Core Aboretum is a 91 acre tract of mesophytic hardwood forest located along the bluffs andbottomlands of the Monongahela River near the WVU campus (https://arboretum.wvu.edu/). The tourwill cover a 2 mile loop, involving some rough and possibly muddy terrain. Parking is available at theWVU Coliseum, and the tour will depart from the Arboretum parking lot at 2 pm. Directions are availablehere: https://arboretum.wvu.edu/#map

Schedule of Sessions and SpeakersSaturday, March 167:00 – 8:00Registration/Check-in/Poster Set-up/Continental Breakfast .South Ag Sci Bldg8:00 – 8:15Welcoming Remarks . . .James H. Arbuckle Lecture Hall8:15 – 8:45Featured Speaker – Dr. Gregg Howe, Michigan State UniversityPlant Immunity to Insect Herbivores: The Dilemma to Grow or Defend8:45 – 10:15Oral Session I .Moderator – Dr. Zhihua Hua, Ohio University8:45T1.Studying the metabolic crosstalk in the plant terpenoid biosynthetic networkErin Hartzell, Division of Plant and Soil Sciences, West Virginia University9:00T2.Acylglucose biosynthesis: Specialized metabolic diversity in wild tomatoDaniel Lybrand, Dept. of Biochemistry and Molecular Biology, Michigan State University9:15T3.Transporting polyamines, amino acids and GABA from the chloroplastMenaka Ariyaratne, Dept. of Biological Sciences, Bowling Green State University9:30T4.Application of CRISPR-Cas9 genome editing technology to discover biological functions of plant cellwall proteins in ArabidopsisYuan Zhang, Dept. of Environmental and Plant Biology, Ohio University9:45T5.Developing a novel protein expression system: Expression of lignolytic enzymes from white-rot fungi inPhyscomitrella patensParul Singh, Dept. of Biology, University of Louisville10:00T6.The biological function of Rab-GTT in Physcomitrella patensHyun Jin Jung, Dept. of Biology, University of Louisville10:15 – 11:30 Poster Session I (Even # Posters) and Refreshments .Agricultural Sciences Bldg.11:30 – 1:00Oral Session II Moderator – Dr. Susanne Hoffmann-Benning, Michigan State University11:30 T7.PLAFP as a phosphatidic acid dependent signal for systemic stress responseAmanda Koenig, Dept. of Biochemistry and Molecular Biology, Michigan State University11:45 T8.Persistant water stress inhibits stomatal development in Betula nigra and Cercis canadensisNoel Mano, Dept. of Botany and Plant Pathology, Center for Plant Biology, Purdue University12:00 T9.Selection of Rhizobacteria with the ability to enhance plant growth under drought and low-nutrientconditionsNathan Nordstedt, Dept. of Horticulture and Crop Science, Ohio State University12:15 T10.In vitro functional analyses of the root-associated bacterial endophytes predict their impact in supportingplant growth in tomatoTri Tien Tran, Dept. of Botany and Plant Pathology, Center for Plant Biology, Purdue University

12:30 T11.Maize and cotton primary root growth responses to water deficit: Comparative physiological andmetabolic analysisJian Kang, Interdisciplinary Plant Group, University of Missouri, Columbia12:45 T12.Determining and comparing hydraulic responses between trees with different wood typesKelsey Bryant, Dept. of Environmental and Plant Biology, Ohio UniversityLunch and Afternoon, March 161:00 – 2:30Lunch . . Agricultural Sciences Bldg.1:30 – 2:20Career Panel Discussion .Lecture Hall G0062:30 – 3:00Featured Speaker - Dr. Steve DiFazio, West Virginia UniversitySex in the Salicaceae3:00 – 4:30Oral Session III .Moderator –Dr. Sen Subramanian, South Dakota State University3:00T13.Transcriptomics identifies modules of differentially expressed genes and novel cyclotides in ViolapubescensAnne Sternberger, Dept. of Environmental and Plant Biology, Ohio University3:15T14.Essential roles of Chlamydomonas galactoglycerolipid lipase PGD1 thylakoid membrane remodeling inresponse to adverse environmental conditionsZhi-Yan Du, Dept. of Biochemistry and Molecular Biology, Michigan State University3:30T15.Functional characterization of nutrient mobilization-related genes in petunia petal senescenceYiyun Lin, Dept. of Horticulture and Crop Science, Ohio State University3:45T16.Profiling genome-wide DNA methylation in identical twin almonds to explore aging and non-infectiousbud-failureKatherine D’Amico-Willman, Dept. of Horticulture and Crop Science, Ohio State University4:00T17.Mapping downy mildew resistance QTL in the progeny of two native North American grapevinesGaurab Bhattarai, Dept. of Biology, Missouri State University4:15T18.The subcellular localization of Ralstonia solanacearum K60 Type III EffectorsRachel Hiles, Dept. of Botany and Plant Pathology, Center for Plant Biology, Purdue University4:30 – 4:45Coffee Break4:45 – 5:45KEYNOTE SPEAKERDr. Elizabeth Haswell, Washington University, St. LouisA Feeling for the Organism: Mechanoperception in the Green Lineage5:45 – 7:15Poster Session II (Odd # Posters) and Appetizers . .Agricultural Sciences Bldg.7:30– 10:00Banquet Dinner and Networking . . Evansdale Crossing Bldg.

Sunday, March 177:00 – 8:00Registration/Check-In/Continental Breakfast .South Ag Sci Bldg8:00 – 9:45Oral Session IV .Moderator – Dr. Nicole Waterland, West Virginia University8:00T19.An evolutionary perspective on recent duplications in the ubiquitin-26S proteasome systemAlexander Bochenek, Dept. of Environmental and Plant Biology, Ohio University8:15T20.Biocontrol fungi in the genus Metarhizium produce ergot alkaloids in a conditionally dependent mannerCaroline Leadmon, Division of Plant and Soil Sciences, West Virginia University8:30T21.Deletion and truncation mutants to probe functional domains of Compromised Hydrolysis ofTriacylglycerols 7 (CHT7) protein in Chlamydomonas reinhardtiiChase Lindeboom, Dept. of Biochemistry and Molecular Biology, Michigan State University8:45T22.Optimizing GmDGAT1b for increasing oil in soybeanKayla Flyckt, Corteva Agriscience, Johnston, Iowa9:00T23.Development of enzymes for robust aryloxyphenoxypropionate and synthetic auxin herbicide tolerancetraits in maize and soybean cropsClayton Larue, Bayer Crop Science9:15T24.Hormonal regulation of defense-related secondary metabolism in polyamine-enriched tomato fruit fieldtested in contrasting ecosystemsTahira Fatima, Dept. of Horticulture and Landscape Architecture, Purdue University9:30 – 10:00Refreshment Break South Ag Sci Bldg10:00 – 10:30 Featured Speaker, Dr. Jean-Michel Ané, University of Wisconsin, MadisonDiscoveries to Improve Nitrogen Fixation in Cereals10:30 – 12:00 Oral Session V. .Moderator – Dr. Gustavo MacIntosh, Iowa State University10:30 T25.Exome sequencing highlights the role of historic wild relative introgression in broadening the adaptivepotential of modern bread wheatFei He, Dept. of Plant Pathology, Kansas State University10:45 T26.Insights into the genetic architecture of growth-defense tradeoffs in a foundation forest tree speciesJennifer Riehl, Dept. of Entomology, University of Wisconsin, Madison11:00 T27.Novel components of plastids with potential roles in plant stress and defenseZeeshan Banday, Dept. of Molecular Genetics and Cell Biology, University of Chicago11:15 T28.Light-induced stabilization of ACS contributes to hypocotyl elongation during the dark-to-light transitionin Arabidopsis seedlingsDong Hye Seo, Dept. of Botany and Plant Pathology, Center for Plant Biology, Purdue University11:30 T29.Control of multicellularity in the moss Physcomitrella patens through modifying ROP-GTPase activityLiang Bao, Dept. of Biology, University of Louisville

11:45 T30.Natural fumigation as a mechanism for volatile transport between flower organsJoseph Lynch, Dept. of Biochemistry, Purdue University12:00 – 12:30 Business Meeting, Announcements, Award Presentations James H. Arbuckle Lecture Hall12:30 pmMeeting ends. Safe travels.Poster TitlesP1.A Scanning Electron Microscopy Technique for Viewing Plant-Microbe Interactions at Tissue and Cell TypeResolutionDenise Caldwell, Department of Botany and Plant Pathology, and Center for Plant Biology, Purdue UniversityP2.What is the role of auxin in root-mediated resistance to Ralstonia solanacearum?Katherine Rivera-Zuluaga, Department of Botany and Plant Pathology, and Center for Plant Biology, PurdueUniversityP3.A Regional Education Program in Sustainable Land Reclamation Management in Central AppalachiaNan Nan, Division of Forestry and Natural Resources, Renewable Materials and Bioenergy Research Center,West Virginia UniversityP4.The role of trichome-derived monoterpenes and sesquiterpenes in the interaction of cultivated tomato and potatoaphidFumin Wang, Division of Plant and Soil Sciences, West Virginia UniversityP5.MiR156 Regulation of Flowering Time of Elevated CO2-Grown PlantsGiavonna Picknally, Department of Biology, Saint Joseph's UniversityP6.Characterizing the Function of PP2-A13 F-box Protein in Plant DevelopmentAbigail G. Moore, Department of Environmental and Plant Biology, Ohio UniversityP7.Quantitative Expression Reduction of Differentiation and Green Like by iCRISPRi Reveals It’s Function inMultiple Metabolic PathwaysPaymon Doroodian, Enviromental and Plant Biology Department, and Interdisciplinary Program in Molecularand Cellular Biology, Ohio UniversityP8.Epigenetic Modifications are Essential for the Rhythmic Control of volatile Organic Compounds Emission inPetunia hybridaShannon Stirling, Department of Biochemistry, and Purdue Center for Plant Biology, Purdue UniversityP9.Time-Course Transcriptomic Analysis of Petunia hybrida ‘Mitchell Diploid’ under Water Deficit Stress UsingRNA SequencingSuejin Park, Division of Plant and Soil Sciences, West Virginia UniversityP10.Using a Poplar Hybrid to Investigate Genetic Control of Associating Insect and Fungal CommunitiesSandra Simon, Department of Biology, West Virginia UniversityP11.Analysis of RIN4 homologs in regulating R-mediated immunity in Important Crop Species

Maheen Alam, Department of Biology, Lahore University of Management SciencesP12.Elucidation of Genetic Components for Acidity Tolerance Using Arabidopsis GWAS PanelMenuka Bhandari, Department of Biology West Virginia State UniversityP13.Compartmentation of Putrescine Synthesis in PlantsKumud Joshi, Department of Biological Sciences, Bowling Green State UniversityP14.Nucleic Acid Programmable Protein Array (NAPPA) Technology as a Functional Genomics Tool for Plant CellWall BiosynthesisMatrika Bhattarai, Department of Environmental and Plant Biology, Ohio UniversityP15.Natural Variation in Arabidopsis thaliana to Discover Candidate Genes in Response to Aluminum ToxicityArjun Ojha Kshetry, Gus R. Douglass Institute and Department of Biology, West Virginia State UniversityP16.The Metabolic and Nutritional Response of Salix spp. to Aluminum StressPhillip Agosti, Biology Department, West Virginia UniversityP17.Exploration into Natural Variation for Detecting Novel QTLs for Arsenic Effects in Arabidopsis thalianaYadira Peña-García, Department of Biology, Gus R. Douglass Institute, West Virginia State UniversityP18.Are timber management practices impacting the insect pollinators in the Allegheny National Forest?Craig Larcenaire, Division of Plant and Soil Sciences, West Virginia UniversityP19.Higher order knock-out mutants of Arabidopsis galactosyltransferases: A reverse genetic approach to revealfunctional roles of O-galactosylation of arabinogalactan-proteins (AGPs)Dasmeet Kaur, Molecular and Cellular Biology Program, Department of Environmental and Plant Biology, OhioUniversityP20.Impact of Simulated Climate Change on Flower Development of PetuniaSarah A. Mills, Division of Plant and Soil Sciences, West Virginia UniversityP21.Genetic regulation of anticancer and neuroprotective glyceollins in soybeanAsraful Jahan, Division of Plant and Soil Sciences, West Virginia UniversityP22.Quantitative Expression Reduction of Differentiation and Green Like by iCRISPRi Reveals Its functioninaNovel Stress Responsive PathwayPaymon Doroodian, Enviromental and Plant Biology Department, and Interdisciplinary Program in Molecularand Cellular Biology, Ohio UniversityP23.Cuticular Composition and Gene Expression Analysis of Cutin Genes by RNA-seq in Habanero Pepper FruitsTolulope Akinmoju, Department of Biology, Gus R. Douglass Institute, West Virginia State UniversityP24.Candidate Genes for Gravity Signal Transduction Identified from Spaceflight ProteomicsProma Basu, Department of Environmental and Plant Biology, Interdisciplinary Program in Molecular andCellular Biology, Ohio UniversityP25.Role of Lipid Binding Proteins and Phosphatidic Acid on Stress Response and Plant DevelopmentBriaunna Murray, Department of Biochemistry and Molecular Biology, Michigan State UniversityP26.Root-Fungal Associations Enhance the Plant Performance of the Invasive Plant Species, Lesser CelandineAllison Paolucci, Deptartment of Environmental and Plant Biology, Ohio UniveristyP27.Three beta-Glucuronosyltransferases impact physiological and biochemical functions of plant cell wallarabinogalactan-proteins in Arabidopsis thaliana

Oyeyemi O. Ajayi, Molecular and Cellular Biology Program, Department of Environmental and Plant Biology,Ohio UniversityP28.De Novo Domestication of Solanum cheesmaniae by Genome Editing Via CRISPR/Cas9: Harnessing SalinityTolerance from a Wild Tomato SpeciesEstefania Tavares Flores, Division of Plant and Soil Sciences, West Virginia UniversityP29.Caffeine Transport in the Coffee Plant: Understanding Caffeine Distribution and Accumulation at the SubcellularLevelRenan T. Pinto, Federal University of Lavras, Brazil, and Division of Plant and Soil Sciences, West VirginiaUniversityP30.Identification of Mobile Transcripts Across the Reciprocal Grafts Involving Watermelon and Bottle gourd byRNA-seq AnalysisMarleny Garcia-Lozano, Department of Biology, Gus R. Douglass Institute, West Virginia State UniversityP31.A Battle of the Regulators: Contrasting Effects of ABA and Transcription Factors on Glyceolin BiosynthesisKiersten Jacobs, Department of Biology, West Virginia UniversityP32.Investigating Floral Transition and Optimizing Germination in Viola pubescensBrett L. Kalfas, Department of Biological Sciences, Ohio UniversityP33.Genetic Improvement of Switchgrass for Energy ProductionSanju Sanjaya, Energy and Environmental Science Institute, Department of Biology, Agriculture ExperimentalResearch Station. West Virginia State UniversityP34.Plant Response Across a Gravitational Gradient Aboard the International Space StationAlexander Meyers, Department of Environmental and Plant Biology, and Interdisciplinary Program inMolecular and Cellular Biology, Ohio UniversityP35.The Effect of Metal Composition and Particle Size on Nanostructure-Toxicity in PlantsNatalie Smith, Department of Biology, Missouri State UniversityP36.Molecular Comparison of the Glyceraldehyde 3-Phosphate Dehydrogenase Gene in Common Ragweed andCuman RagweedDave L. Robinson, Department of Biology, Bellarmine UniversityP37.Towards an improved metric of Cryptophonectria parasitica infection to quantify species based responses toinfectionSam Kukor, Department of Environmental and Plant Biology, Ohio UniversityP38.Gene Expression at the Intersection of Microgravity and Reorientation Implicates New Regulators of GravitySignalingCalvin M. Coffin, Department of Environmental and Plant Biology, and Interdisciplinary Program in Molecularand Cellular Biology, Ohio UniversityP39.Patterns of Recombination and Segregation Distortion in the Populus GenomeChanaka Roshan Abeyratne, Department of Biology, West Virginia UniversityP40.Evolutionary history and diverse functions of NODULE INCEPTION-LIKE proteins (NLPs) in root developmentNarender Kumar, Department of Botany and Plant Pathology, Purdue UniversityP41.Comparative GWAS for Seedling Root Variation in Acidic Conditions across the Pepper Species ComplexesTosin Akinsipe, Department of Biology, Gus R. Douglass Institute, West Virginia State University

P42.Functional analysis of MtMATE30, a nodule-specific membrane effluxer in the model legume MedicagotruncatulaLina Yang, Division of Plant and Soil Sciences, West Virginia UniversityP43.The Effects of Carbon-Based Nanoparticles on Tomato Germination and GrowthSusan Eiben, Department of Environmental and Plant Biology, Ohio UniversityP44.Understanding genetic and environmental factors to enable cultivation of winter malting barley in Ohio and acrossthe MidwestBen Eggers, Department of Horticulture and Crop Science, Ohio State UniversityP45.Physiological and Metabolomic Responses of Black Cottonwood to Water StressMarvin W. Wright, Department of Biology, West Virginia University

List of PresentersLast NameFirst NameTalk or PosterLast rinFeiRachelKierstenAsrafulKumudHyun JinBrettJianDasmeetAmandaSamP39P16P27P23P41P11T3T 27T 29P24P12T 17P14T 19T 12P1P38T 16P7, P22T 14P44P43T 24T 22P30T1T 25T 18P31P21P13T6P32T jha KshetryPaolucciParkPena ayaSeoSimonSinghSmithSternbergerStirlingTavares FloresTranWangWrightYangZhangFirst DaveSanjuDong arvinLinaYuanTalk or PosterP40P18T 23T 20T 15T 21T2T 30T8P34P20P6P25P3T9P15P26P9P17P5P29T 26P2P36P33T 28P10T5P35T 13P8P28T 10P4P45P42T4

List of RegistrantsLast lfasKangKangiKaurKleinsasserKoenigFirst achelSusanneGreggZhihuaKierstenMd il o.edujlk0037@mix.wvu.edukoenigam@msu.edu

Last z ayNanNordstedtOjha KshetryPainPanaccionePaolucciParkPena Tavares dYangYuZhangFirst nDong nEmail .edu

Evansdale Crossing Bldg Vegetarian options will be available as part of all meals provided; however, preference will be given to individuals who requested vegetarian meals during meeting registration. Posters: Posters need to be mounted on poster boards between 7:00

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