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Instructions for Use fori-controlDocument Part No.: 300186682012-08Document Version No.: 2.0Software Version No.: 1.10

Tecan Customer SupportTecan Asia Pte Ltd.Tecan Australia Pty Ltd.AddressContact18 Boon Lay Way,#09-140 TradeHub 21Singapore 609966Singapore21 / 3 Westside AvenuePort Melbourne Vic 3207AustraliaT 65 6444 1886F 65 6444 [email protected] 1300 808 403 (free in Australia)T 61 3 9647 4100F 61 3 9647 [email protected] Austria GmbHUntersbergstr. 1AA-5082 GrödigAustriaBusinesspark E19Battelsesteenweg 455 B2800 MechelenBelgiumIndustrieweg 30NL-4283 GZ GiessenNetherlandsWerner-von-Siemens-Straße 23D-74564 CrailsheimGermanyTecan Benelux bvbaTecan Benelux bvbaTecan Deutschland GmbHTecan France S.A.S.Tecan Ibérica Instrumentation S.L.(Spain)Tecan Ibérica (Spain)Tecan Ibérica (Portugal)Tecan Italia S.r.l.Tecan Japan Co. LtdTecan Nordic ABTecan (Shanghai) Trading Co., Ltd.Tecan Schweiz AGTecan Systems Inc.Technical support forcomponentsTecan UKTecan USTechnical support forTecan instruments226 avenue Tony GarnierF-69007 LyonFranceGran Via de Carlos III, 98, planta 10Edificios Trade (Torre Norte)08028 Barcelona, SpainN VI - km 23,300,c/ Pollensa, 4 - Oficina 8E-28230 Las Rozas de MadridSpainQuinta da Fonte Edificio Pedro IP-2780-730 Paço D'ArcosPortugalVia Brescia, 39I-20063 - Cernusco Sul Naviglio (MI)ItalyKawasaki Tech Center580-16, Horikawa-cho, Saiwai-kuKawasaki, Kanagawa 212-0013JapanTaljegårdsgatan 11BSE-431 53 MölndalSwedenRoom 1802-1804, No.388,Fushan Road, Pudong New Area200122 Shanghai, P.R. ChinaSeestrasse 103CH-8708 MännedorfSwitzerland2450 Zanker RoadSan Jose, CA 95131USATheale Court11-13 High StreetThealeUK-Reading RG7 5AHUnited Kingdom9401 Globe Center DriveSuite 140Morrisville, NC27560 USAT 43 6246 8933 256F 43 6246 72 [email protected] 32 15 709 054 (English)T 32 15 709 055 (Fench)F 32 15 421 [email protected] 31 20 708 4773F 31 183 44 80 [email protected] 49 1805 8322 633 orT 49 1805 TECAN DEF 49 7951 9417 [email protected] 33 820 88 77 36F 33 4 72 76 04 [email protected] 34 93 49140 26F 34 93 33087 [email protected] 34 91 151 71 07F 34 91 151 [email protected] 35 21 000 8216T 39 800 11 22 91F 39 (02) 92 72 90 [email protected] 81 44 556 7311(Kawasaki)F 81 44 556 7312 (Kawasaki)T 81(0) 6305 8511 (Osaka)[email protected] 46 317 54 40 00F 46 317 54 40 [email protected] 86 21 2206 3206T 86 21 4008213888F 86 21 2206 [email protected] 41 44 922 82 82F 41 44 922 89 [email protected] 1 408 953 3100F 1 408 953 3101Toll Free in the US:T 1 866 798 [email protected] 44 118 930 0300F 44 118 930 [email protected] 1 919 361 5200F 1 919 361 5201Toll Free in the US:T 1 800 TECAN US orT 1 800 832 [email protected] for Use for i-control No. 30018668 Rev. No. 2.02012-08

Warnings, Cautions, and NotesThe following types of notices are used in this publication to highlight importantinformation or to warn the user of a potentially dangerous situation:NoteGives helpful information.CAUTIONINDICATES A POSSIBILITY OF INSTRUMENT DAMAGE OR DATALOSS IF INSTRUCTIONS ARE NOT FOLLOWED.WARNINGINDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY,LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONSARE NOT FOLLOWED.TrademarksThe following product names and any registered and unregistered trademarksmentioned in this document are used for identification purposes only and remainthe exclusive property of their respective owners: i-control , magellan , Infinite , MultiCheck , NanoQuant Plate , Tecanand the Tecan Logo are registered trademarks of Tecan Group Ltd.,Männedorf, SwitzerlandTMTM TMTM Windows and Excel are registered trademarks of Microsoft Corporation,Redmond, WA, USA BRET2 TMis a trademark of Perkin Elmer Corporation, MA, USA Chroma-GloTM is a trademark of Promega Corporation, WI, USA Greiner and µClear and are registered trademarks of Greiner LabortechnikGmbH, Frickenhausen, Germany HTRF is a registered trademark of CisBio International, France Hellma is a registered trademark of Hellma GmbH & Co. KG, Müllheim,Germany AlphaScreen and AlphaLISA are registered trademarks of Perkin Elmer,Inc., Waltham, USA2012-08Instructions for Use for i-control No. 30018668 Rev. No. 2.03

WARNINGCAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDEDIN THIS MANUAL BEFORE OPERATING THE INSTRUMENT.NoticeEvery effort has been made to avoid errors in text and diagrams; however, TecanAustria GmbH assumes no responsibility for any errors which may appear in thispublication.It is the policy of Tecan Austria GmbH to improve products as new techniquesand components become available. Tecan Austria GmbH therefore reserves theright to change specifications at any time with appropriate validation, verification,and approvals.We appreciate any comments on this publication.ManufacturerTecan Austria GmbHUntersbergstr. 1AA-5082 Grödig, AustriaT: 43 6246 8933F: 43 6246 72 770www.tecan.comE-mail: [email protected] InformationThe contents of this manual are the property of Tecan Austria GmbH and are notto be copied, reproduced or transferred to another person or persons withoutprior written permission.Copyright Tecan Austria GmbHAll rights reserved.Printed in Austria.About the Instructions for UseThis document describes i-control, which is a software to control Infinite SeriesTecan microplate readers. It is intended as a reference and instruction for theuser.This manual instructs how to: Install the software Operate the softwareRemarks on ScreenshotsData and parameters displayed in screenshots vary depending on the instrumentconnected. Details and examples are described in the respective Instructions forUse of the connected instrument.4Instructions for Use for i-control No. 30018668 Rev. No. 2.02012-08

Table of Contents1. Introduction . 71.11.21.31.4Area of Application . 71.1.1 i-control Intended Use . 7Specifications . 71.2.1 Hardware Requirements . 71.2.2 Reader Compatibility . 91.2.3 CE Declaration for Europe . 9Software Installation . 101.3.1 Software Installation under Windows Vista, Windows 7 . 111.3.2 Hardware Wizard (only valid for WindowsXP) . 13Starting i-control . 161.4.1 Connected Instrument . 161.4.2 Simulated Instrument . 182. Measurement Parameter Editor . 212.12.22.32.4Introduction . 21Control Bar. 222.2.1 Lab Ware . 232.2.2 Measurements . 252.2.3 Actions . 462.2.4 Kinetic . 512.2.5 Miscellaneous . 53Workflow Pane. 552.3.1 Hierarchy of Elements . 56Info Pane . 563. Defining Measurements . 573.13.23.33.4Defining End Point Measurements . 573.1.1 Plate Size – Part of the Plate . 59Defining Multilabel Measurements . 60Defining Kinetic Measurements . 643.3.1 Defining Well Kinetic Measurements with Injections . 663.3.2 The Difference between “Inject” and “Dispense” . 67Indenting and Releasing Program Elements . 683.4.1 Ways to Indent or Release Program Elements . 694. Menus . 704.12012-08Menu4.1.14.1.24.1.34.1.44.1.54.1.6Bar . 70File Menu . 70Edit Menu . 71View Menu. 71Instrument Menu. 72Settings Menu . 75Help Menu . 91Instructions for Use for i-control No. 30018668 Rev. No. 2.05

4.2Toolbar . 925. Batch Processing . 955.15.25.35.45.5Introduction . 95Microplate Requirements for Batch Processing . 95Start Stacker Run . 96Restacking . 97Stacker Kinetics (available for Infinite F500, M1000 andM1000 PRO) . 976. Gas Control Module (GCM) Enhanced Support . 996.16.26.36.46.56.66.7Introduction . 99Prerequisites . 99Connecting to GCM Enhanced . 100Data Logging . 1006.4.1 Importing Logged Data Into Microsoft Excel . 102GCM Enhanced Data Displayed in Status Bar . 102GCM Enhanced Data Displayed in Excel . 103Precautions Before Starting a Measurement . 103Index . 1056Instructions for Use for i-control No. 30018668 Rev. No. 2.02012-08

1. Introduction1.Introduction1.1 Area of Applicationi-control is an easy-to-use and flexible tool, which gives the user complete controlover Tecan readers.i-control presents the raw data for further use in Excel, offering excellent featuresfor research purposes.NoteDepending on the instrument connected and the modules equipped,certain i-control features may be disabled or invisible.1.1.1i-control Intended UseThe i-control software is a general-purpose software accessory to a TecanInfinite Series reader, designed for professional use according to the softwarespecifications.i-control is designed for use with Excel for data presentation.1.2 Specifications1.2.1Hardware RequirementsThe following hardware requirements and operating system requirements have tobe met to use the i-control dWindows XP (32-bit):Windows compatible PC with aPentium compatible processorrunning at 1 GHz2 GHz(Dual Core)Windows Vista (32 bit):Windows compatible PC with aPentium compatible processorrunning at 1 GHz2 GHz(Dual Core)Windows 7 (32- or 64-bit):Windows compatible PC with aPentium compatible processorrunning at 1 GHz2 GHz(Dual Core)Windows XP (32-bit) SP3Windows Vista (32-bit)Windows 7 (32-bit)Windows 7 (64-bit)Windows XP(32-bit) SP3Instructions for Use for i-control No. 30018668 Rev. No. 2.07

1. IntroductionMemoryMinimumRecommendedWindows XP:512 MB RAM1 GB RAMWindows Vista (32-bit):1 GB RAM2 GB RAMWindows 7 (32-bit):1 GB RAMWindows 7 (64-bit):2 GB RAMSpaceRequirements700 MBMonitorSuper VGA GraphicsResolution1024 x 768Color Depth256MouseMicrosoft mouse or compatiblepointing deviceCommunication1 x USB 2.0Devices1 x CD-ROM drive2 GB RAM3 GB RAM1 GB1280 x 10242 x USB 2.01 x RS232(Serial)Windows Vista:DirectX 9 graphics and 32 MB ofgraphics memory (for Home Basic);128 MB of graphics memory plusWDDM support for all otherversionsWindows 7:DirectX 9 graphics device withWDDM 1.0 or higher driver8.NETMicrosoft .NET Framework 2.0If this version is not present, theinstall/upgrade program will install itside-by-side with any existinginstallation of the .NET Framework.WindowsInstaller3.1If this version is not present, theinstall/upgrade program will install it.Microsoft Excel2002200320072010 (32-bit) – Starter edition NOTsupported!Instructions for Use for i-control No. 30018668 Rev. No. 2.020032012-08

1. Introduction1.2.2Reader CompatibilityThe following Tecan readers can be used with i-control:Instrument TypesMeasurement ModeInfinite M200Infinite M200 PROFluorescence / Absorbance / LuminescenceInfinite F200Fluorescence / Absorbance / Luminescence /Fluorescence PolarizationInfinite F200 PROFluorescence / Absorbance / Luminescence /Fluorescence Polarization / AlphaScreen/AlphaLISAInfinite F500Fluorescence / Absorbance / Luminescence /Fluorescence PolarizationInfinite M1000Fluorescence / Absorbance / Luminescence /Fluorescence PolarizationInfiniteM1000 PROFluorescence / Absorbance / Luminescence /Fluorescence Polarization / AlphaScreen/AlphaLISAInfinite F50AbsorbanceNoteThe Connect stacker can be used together with several instruments inorder to measure batches of plates. Please refer to the ConnectInstructions for Use for more information.With the Infinite M1000 and Infinite M1000 PRO instruments, only thebuilt-in stacker can be used.1.2.3CE Declaration for Europei-control is not a CE-marked product. Therefore no CE declaration for Europe isavailable.2012-08Instructions for Use for i-control No. 30018668 Rev. No. 2.09

1. Introduction1.3 Software InstallationCAUTIONYOU MUST HAVE ADMINISTRATIVE RIGHTS TO INSTALL THESOFTWARE.CAUTIONINSTALL THE SOFTWARE BEFORE PLUGGING THE INSTRUMENTINTO THE COMPUTER.The i-control software is installed using the following procedure:1.2.3.Insert the installation CD into the appropriate disk drive or CD ROM drive.Open the Windows Explorer and browse to folder Software on theinstallation CD. Double-click i-Control version .exe to start theinstallation procedure.First of all Setup Prerequisites have to be installed:Depending on your operating system different prerequisites have to be installed.Click Next to continue.10Instructions for Use for i-control No. 30018668 Rev. No. 2.02012-08

1. Introduction4.A message box indicates that the prerequisites have been installedsuccessfully. Click OK to continue.5In the course of the installation a series of dialog boxes will appear. Readeach one, enter any necessary information and click Next to continue.The files are installed and the program icon is created.When the Installation Complete dialog box appears, click Finish andthe i-control program is ready to be used.6.1.3.1Software Installation under Windows Vista, Windows 7When installing the software under Windows Vista, for security reasons, the userhas to decide whether to install the device driver software or not.The following dialogs appear (example):Click Install on both to continue.2012-08Instructions for Use for i-control No. 30018668 Rev. No. 2.011

1. IntroductionIn the bottom right screen corner, the operating system informs you on theprogress of installation:Clicking Click here for status and the system displays in detail which driver hasbeen installed. The following window appears:If Click here for status is not clicked, several windows appear with information inappearing and fading balloons about the current status of the installation (thisscreenshot shows the last balloon, confirming successful installation of thesoftware):12Instructions for Use for i-control No. 30018668 Rev. No. 2.02012-08

1. Introduction1.3.2Hardware Wizard (only valid for WindowsXP)If the instrument is plugged in after the software has been installed, the followingHardware Wizard dialog boxes appear:Depending on system configuration and installed drivers, this dialog box mayappear first:Select No, not this time and click Next.Select Install the software automatically and click Next.2012-08Instructions for Use for i-control No. 30018668 Rev. No. 2.013

1. IntroductionThe Hardware Wizard searches for the device.After the device has been found, click Next.Depending on existing previous drivers, the following dialog box appears:The wizard suggests the appropriate device.Select Next to complete the New Hardware Wizard.14Instructions for Use for i-control No. 30018668 Rev. No. 2.02012-08

1. IntroductionClick Finish to complete installation. The software is ready for use.2012-08Instructions for Use for i-control No. 30018668 Rev. No. 2.015

1. Introduction1.4 Starting i-controli-control can be used either with a connected instrument or in simulation mode.1.4.1Connected InstrumentCAUTIONINSTALL THE SOFTWARE BEFORE CONNECTING THE INSTRUMENTTO THE COMPUTER.Connect the instrument to your computer and switch the instrument on. Start theprogram by selecting Programs/Tecan/i-control from the Windows Start menu.Select Connect from the Instrument menu or click the connect buttonthe following dialog box appears:Example for the Infinite 200 instrument:andIn the Connect to: dialog box select the instrument name.In the Additionally connect to: field, select Connect, if a Connect stacker isconnected (for batch processing).16Instructions for Use for i-control No. 30018668 Rev. No. 2.02012-08

1. IntroductionExample for the Infinite M1000 instrument:In the Connect to: dialog box select the instrument name.Connect built-in stacker:With the Infinite M1000 and Infinite M1000 PRO instruments, only the built-instacker can be used (see screenshot).Click OK to start i-control.2012-08Instructions for Use for i-control No. 30018668 Rev. No. 2.017

1. Introduction1.4.2Simulated InstrumentStart the program by selecting Programs/Tecan/i-control from the WindowsStart menu. In the Connect to Instrument dialog box, select Show simulatedinstruments; from the Instrument Name list, select the demo instrument toconnect to.After selecting the simulated instrument, a drop-down list appears offering severaloptions, depending on the instrument selected above.For the Infinite 200, for example, these options are: Filter: F200 normal or F200 enhanced or F200 with FP Option Monochromator: M200 normal or M200 enhancedFor the Infinite 200 PRO, for example, these options are: F200PRO (PMT NORMAL) F200PRO (PMT ENHANCED) F200PRO ALPHA F200PRO WITH FP OPTION (PMT NORMAL) M200PRO (PMT NORMAL) M200PRO (PMT ENHANCED)For the Infinite F500, for example, these options are: GF500 (PMT NORMAL) 384 GF500 (PMT ENHANCED) 1536/384 FI.TOP/ABS/HEA/SHK ONLY (PMT Normal) 1536/384 GF500 WITH FP (PMT NORMAL) 38418Inst

France T +33 820 88 77 36 F +33 4 72 76 04 99 [email protected] Tecan Ibérica Instrumentation S.L. (Spain) Gran Via de Carlos III, 98, planta 10 Edificios Trade (Torre Norte) 08028 Barcelona, Spain T +34 93 49140 26 F +34 93 33087 00 [email protected] Tecan Ibérica (Spain) N VI - km 23,300, c/ Pollensa, 4 - Oficina 8