Thermo Scientific Orion - Thermo Fisher Scientific
U ser ManualThermo Scientific OrionROSS Ultra, ROSS Ultra Triodeand ROSS pH Electrodes263745-001 Revision B November 2014
ContentsIntroduction4ROSS Electrode Families4Required Equipment5Electrode Preparation6Sample Requirements8Measuring Hints9Electrode Calibration10pH Measurements12Electrode Storage12Electrode Maintenance12Electrode Cleaning Procedures12Electrode Characteristics13ROSS Ultra ElectrodesROSS Ultra pH/ATC Triode ElectrodesROSS ElectrodesROSS Sure-Flow ElectrodesROSS Semi-micro ElectrodesROSS micro pH ElectrodesPreparation for Refillable ROSS ElectrodesPreparation for ROSS Sure-Flow ElectrodesPreparation for Gel-Filled ROSS Ultra Triode ElectrodesGeneral Calibration ProcedureOne Buffer CalibrationTwo Buffer CalibrationGeneral CleaningCleaning SolutionsTemperature EffectsSure-Flow Electrode Leak RateROSS Micro pH ElectrodeROSS Ultra pH/ATC Triode Electrodes445555678101011121313141415ROSS Electrode Operation with Orion Versa Star pH/LogR Meters15Electrode ty18RoHS Statement18Ordering Information19Specifications21pH MeterElectrodeSample/ApplicationTechnique17171717
IntroductionThis user manual contains information on the preparation, operationand maintenance of the Thermo Scientific Orion ROSS Ultra ,Thermo Scientific Orion ROSS , and Thermo Scientific Orion ROSS Triode electrodes.All ROSS pH electrodes have a pH range of 0 to 14, pH precision of0.01 and temperature range of 0 to 100 C. ROSS electrodes can beused in samples that contain TRIS, sulfides and proteins since they donot contain silver or mercury.ROSS electrodes incorporate the distinctive ROSS internal referencesystem that provides superior measurement stability, faster response,greater accuracy and more reproducible results than conventionalelectrodes. ROSS electrodes have a double junction reference, sothe fill solution can be modified to more closely match the samplecomposition in high pH, low pH or non-aqueous solutions.ROSS electrodes provide readings stable to 0.01 pH in lessthan 30 seconds, even in the extreme case of samples varying fromone another by 50 C or more. Results are three to five times moreprecise than those obtained with conventional electrodes. Over thelifetime of the electrode, ROSS reference potential drifts less than anaverage 0.12mV per day, which corresponds to 0.002 pH units, sorecalibration is minimized. The majority of ROSS electrodes with a BNC waterproof or BNCconnector have a one meter (three foot) long cable, except wherenoted. All electrodes with a screw cap connection require a separate cable. Electrodes with a waterproof BNC connector can be used on any pHmeter with a BNC connection. The 800500U ROSS Ultra reference half-cell and 800300 ROSSreference half-cell can be used with the 8101BNWP and 8101SCROSS pH half-cell electrodes or 8411BN ROSS sodium half-cellelectrode.ROSS Electrode FamiliesROSS Ultra ElectrodesROSS Ultra electrodes offer the ultimate measurement stability and theleast long-term drift, plus a two year warranty for refillable models andan 18 month warranty for gel-filled models.ROSS Ultra pH/ATC Triode ElectrodesROSS Ultra pH/ATC Triodes have a built-in temperature probe,so pH and temperature are measured with one electrode.Gel-filled ROSS Ultra pH/ATC Triode electrodes have an 18 monthwarranty and refillable ROSS Ultra pH/ATC Triode electrodes have a 24month warranty.4 ROSS Ultra, ROSS Ultra Triode and ROSS pH ElectrodesThermo Scientific Orion
ROSS ElectrodesROSS electrodes provide precise pH measurements and measurementstability regardless of temperature changes or sample composition.ROSS Sure-Flow ElectrodesROSS Sure-Flow electrodes incorporate a free-flowing junction that iseasy to clean and never clogs. These electrodes are ideal for dirty orviscous samples that clog traditional electrodes.ROSS Semi-micro ElectrodesROSS semi-micro electrodes are designed for applications wheresample volume is limited and can take measurements in samples of 0.2ml volume. They are available with chemically-resistant glass (6 mmtip) or epoxy (8 mm tip) bodies, Sure-Flow junction and ROSS Ultralong-life models.ROSS micro pH ElectrodesROSS micro pH electrodes have been designed to measureextremely small samples. The measuring tip is 3 mm in diameter and40 mm long. The minimum depth of immersion is 4.5 mm and theminimum sample size is 15 μL in 384 well plates.Required Equipment1. Thermo Scientific Orion Versa Star , Thermo Scientific OrionStar A300 series, Thermo Scientific Orion Star A200 series,Thermo Scientific Orion Star A100 series or Thermo Scientific Orion Dual Star meter with pH measurement capability,such as the Versa Star benchtop meter with pH module, StarA211 pH benchtop meter or Star A321 pH portable meter;except 8107BNURCA, 8157BNURCA and 8302BNURCA Triodeelectrodes which are designed for use with a meters which supporta thermister with a 30K NTC curve and have an RCA plug, such asthe Five and Seven Series METTLER TOLEDO pH meters.ROSS electrodes can be used on any pH meter with a BNCconnection. The electrodes can also be used on meters witha variety of inputs when an adapter cable is used. Visit www.thermoscientific.com/water for details.2. Orion ROSS Ultra, and ROSS Triode electrodes. The 8302BNUMD, 8107BNUMD, 8107UWMMD, 8157BNUMDand 8157UWMMD pH/ATC Triode electrodes have atemperature connector that is compatible with Orion Star Aseries, Versa Star and Dual Star pH meters. The 8107BNURCA, 8157BNURCA and 8302BNURCA pH/ATC Triode electrodes have a temperature connector that iscompatible with meters which support a thermister with a 30KNTC curve and have an RCA plug, such as the Five and SevenSeries METTLER TOLEDO pH meters.3. ROSS fill solution, Cat. No. 810007.Note: Do not use any fill solution that contains silver, as silverwill damage ROSS electrodes.Thermo Scientific OrionROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes 5
Note: The 8107BNUMD, 8107UWMMD and 8107BNURCAelectrodes are gel-filled and do not require any fill solution.4. ROSS pH electrode storage solution, Cat. No. 810001, or standardpH electrode storage solution, Cat. No. 910001. For enhancedelectrode performance, use ROSS pH electrode storage solution.5. pH buffers – at least two pH buffers are recommended for precisemeasurements. One buffer should be near pH 7 and buffers shouldbe one to four pH units apart.6. Beakers, plastic or glass.7. Magnetic stirrer or Orion stirrer probe, Cat. No. 096019. The stirrerprobe can be used with Orion Star A series, Versa Star and DualStar benchtop meters.8. Distilled or deionized water.Electrode PreparationPreparation for Refillable ROSS Electrodes1. Remove the shipping cap, sleeve or bottle from the sensing bulband save it for later storage of the electrode.2. Clean any salt deposits from the exterior of the electrode by rinsingit with distilled water. Handle the 8220BNWP ROSS micro pHelectrode with great care.3. The electrode may arrive with the chamber partially filled withelectrolyte solution. Please fill the chamber with electrolyte solutionbefore use. Uncover the fill hole by removing the tape and addROSS fill solution, Cat. No. 810007, to the electrode up to the fillhole. To maintain an adequate flow rate, the level of fill solutionmust always be above the reference junction and at least one inchabove the sample level. The fill hole should remain open wheneverthe electrode is in use.4. Place the electrode in an electrode holder and suspend theelectrode in air for 5 minutes to thoroughly wet the referencejunction. Omit this step for electrodes which come shipped in astorage bottle.5. Shake the electrode downward (similar to a clinical thermometer)to remove any air bubbles. The 8220BNWP electrode is extremelyfragile. Do not shake the 8220BNWP electrode unless there areair bubbles in the fill solution and then shake gently and with greatcare.6. Soak electrode in ROSS pH electrode storage solution,Cat. No. 810001, or standard pH electrode storage solution, Cat.No. 910001, for 30 to 60 minutes. ROSS Ultra Triode electrodesdo not need to be soaked prior to use, since they are shipped in astorage bottle with storage solution.7. Connect the electrode to the meter.6 ROSS Ultra, ROSS Ultra Triode and ROSS pH ElectrodesThermo Scientific Orion
Fill ncejunctionpH sensingbulbROSS Refillable Combination pH ElectrodePreparation for ROSS Sure-Flow Electrodes1. Remove the shipping cap, sleeve or bottle from the sensing bulband save it for later storage of the electrode.2. Clean any salt deposits from the exterior of the electrode by rinsingit with distilled water.3. Uncover the fill hole by removing the tape and add ROSS fillsolution, Cat. No. 810007, to the electrode up to the fill hole. ToFill holemaintain an adequate flow rate, the level of fill solution must alwaysbeabove the reference junction and at least one inch above theFill holesample level. The fill hole should remain open whenever theelectrode is in use. Fill solutionROSSinternalchamber4. Wetreferencethe junction by pressingdown on the electrode cap to let a fewFillsolutiondropsof the ROSS fillsolution flow out of the electrode and thenSure-FlowROSSchamberreferencepH sensingany lost fill solution.internalreplenishjunctionreferencebulb5. Shake the electrodeReferencedownward (similar to a clinical thermometer) tojunctionremove any air bubbles.pH sensingbulb6. Soak electrode in ROSS pH electrode storage solution, Cat.No. 810001, or standard pH electrode storage solution, Cat.No. 910001, for 30 to 60 minutes. For enhanced electrodeperformance, use ROSS pH electrode storage solution.7. Connect the electrode to the meter.Fill holeROSSinternalreferencepH sensingbulbThermo Scientific OrionFill solutionchamberSure-FlowreferencejunctionROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes 7
Preparation for Gel-Filled ROSS Ultra TriodeElectrodes1. Remove the electrode storage bottle from the sensing bulb andsave it for later storage of the electrode.Note: Unscrew the cap on the electrode storage bottle beforeremoving the electrode.2. Clean any salt deposits from the exterior of the electrode by rinsingit with distilled water.3. Connect the electrode to the meter.4. If the electrode will not be used immediately, soak it in ROSSpH electrode storage solution, Cat. No. 810001, or standard pHelectrode storage solution, Cat. No. 910001.ROSS Gel-filled Combination pHElectrodeROSSinternalreferenceGel solutionchamberReference junctionpH sensing bulbSample RequirementsA benefit of the ROSS pH electrodes is that the fill solution compositionmay be changed depending on the sample requirements. The ROSSpH electrode fill solution, Cat. No. 810007, is 3 M KCl. For solutionsthat precipitate in the presence of chloride ions, the electrode can befilled with 10% KNO3, Cat. No. 900003.Electrodes with an epoxy body should only be used in aqueoussolutions.Electrodes with a glass body may be used in non-aqueous solutionsand solutions that contain organic solvents. A minimum of 20% watermust be present in the sample for the best measurement results. Ifthere is a great deal of drift when using the ROSS electrode filled withROSS fill solution, try filling the electrode with a mixture of methanol8 ROSS Ultra, ROSS Ultra Triode and ROSS pH ElectrodesThermo Scientific Orion
and water saturated with KCl. The ratio of methanol to water should besimilar to the sample composition. For tips on measuring pH in nonaqueous samples, see Thermo Scientific Water Analysis InstrumentsApplication Note 007.If using the 8220BNWP ROSS micro pH electrode, great care mustbe taken to thoroughly rinse out the reference chamber and junctionbefore changing the reference solutions.Measuring Hints Always use fresh buffers for calibration. Choose buffers that are oneto four pH units apart. Check the electrode slope daily by performing a two buffercalibration. The slope should be 92 to 102%. Except as noted in the Sample Requirements section, only useROSS fill solution, Cat. No. 810007. Do not use any fill solution thatmay contain silver. If a refillable electrode is used, always remove the fill hole coverduring measurements to ensure a uniform flow of fill solution. If a ROSS Ultra Triode is used, immerse the electrode at least 2inches (5 cm) in solution for best temperature response. Between measurements, rinse the electrode with distilled water andthen with the next solution to be measured. Stir all buffers and samples at a uniform rate. Place a piece of insulating material, such as polystyrene foam orcardboard, between the magnetic stir plate and beaker to preventmeasurement errors from the transfer of heat to the sample. SinceROSS electrodes respond quickly to temperature changes, pHchanges due to temperature variations will be noticed. To reduce the chance of error due to polarization, avoid rubbing orwiping the sensing bulb. Use a lint-free tissue and gently blot thesensing bulb. If a refillable electrode is used in dirty or viscous samples orthe electrode response becomes sluggish, empty the electrodecompletely and hold the junction under running water. Empty anywater from the electrode and refill it with fresh ROSS fill solution.For a thorough cleaning method, refer to the Electrode CleaningProcedures section. If a Sure-Flow electrode is used in dirty or viscous samples orthe electrode response becomes sluggish, empty the electrodecompletely and hold the junction open under running water. Emptyany water from the electrode and refill it with fresh ROSS fillsolution. Press down on the electrode cap to let a few drops of theROSS fill solution flow out of the electrode and then replenish anylost solution. For a thorough cleaning method, refer to the ElectrodeCleaning Procedures section. Handle the 8220BNWP ROSS micro pH electrode with care. Donot touch the pH bulb and stem against the bottom or walls of thesample containers.Thermo Scientific OrionROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes 9
Filling hole shouldbe uncoveredFilling solution levelmust be higher thansample levelReference junctionmust be immersedFresh pH bufferPlace insulationbetween stirrerand beakerStir all samplesand buffersMeasuring Hints Flat surface ROSS pH electrodes may be used on anymoist surface or in liquids.Using a Flat Surface ElectrodeElectrode CalibrationGeneral Calibration ProcedureFor detailed instructions on pH calibration, manual pH calibration andtemperature compensation, consult your meter user guide. Whenusing ROSS electrodes with a Versa Star pH/LogR meter, refer to theVersa Star pH/LogR meter user guide for instructions on temperaturecalibration and LogR temperature compensated pH measurements.Note: If the meter is set to automatically recognize the pH buffer at themeasured temperature and an ATC probe is used, you do not need tomanually adjust the pH values of the buffers.One Buffer Calibration1. Choose a buffer near expected sample pH.2. The buffer should be at same temperature as the sample. If thebuffer and samples are at varying temperatures, temperaturecompensation is recommended.3. Prepare the meter according to the meter user guide.4. Rinse the electrode first with distilled water and then with the bufferbeing used for calibration.10 ROSS Ultra, ROSS Ultra Triode and ROSS pH ElectrodesThermo Scientific Orion
5. Place the electrode into the buffer. When the reading is stable,set the meter to the pH value of the buffer at the measuredtemperature. Refer to the meter user guide for a detailedprocedure. Table 1 provides pH buffer values at varioustemperatures.6. Proceed to the pH Measurement section.Two Buffer CalibrationThis procedure is recommended for precise measurements.1. Select two buffers that bracket the expected sample pH. The firstbuffer should be near the electrode isopotential point (pH 7) andthe second should be near the expected sample pH (pH 4 or pH10). Buffers should be one to four pH units apart.2. The buffers should be at same temperature as the sample. If thebuffers and samples are at varying temperatures, temperaturecompensation is recommended.3. Rinse the electrode first with distilled water and then with the firstbuffer.4. Place the electrode into the first buffer. When the reading isstable, set the meter to the pH value of the buffer at the measuredtemperature. Refer to the meter user guide for a detailedprocedure. Table 1 provides pH values at various temperatures.5. Rinse the electrode first with distilled water and then with thesecond buffer.6. Place the electrode into the second buffer. When the readingis stable, set the meter to the pH value of the buffer at themeasured temperature. Refer to the meter user guide for adetailed procedure. Table 1 provides pH buffer values at varioustemperatures.90 C80 C70 C60 C50 C40 C30 C20 C10 C5 CTemperature0 CNominalBufferValue at25 C1.681.67 1.67 1.67 1.68 1.68 1.69 1.71 1.72 1.74 1.77 1.794.014.00 4.00 4.00 4.00 4.02 4.03 4.06 4.09 4.12 4.16 4.216.866.98 6.95 6.92 6.87 6.85 4.03 6.83 6.84 6.85 6.86 6.887.007.11 7.08 7.06 7.01 6.98 6.97 6.96 6.97 6.99 7.03 7.089.189.46 9.40 9.33 9.23 9.14 9.07 9.01 8.96 8.92 8.89 8.8510.0110.32 10.25 10.18 10.06 9.97 9.89 9.83 9.79 9.78 9.78 9.8012.4613.47 13.24 13.03 12.64 12.29 11.99 11.73 11.50 11.30 11.13 11.98Table 1: pH Values of Buffers at Various TemperaturesThermo Scientific OrionROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes 11
pH Measurements1. Calibrate the electrode as described in the Electrode Calibrationsection.2. Rinse the electrode with distilled water and then with the sample.3. Place the electrode into the sample.4. When the reading is stable, record the pH and temperature of thesample.Electrode StorageTo ensure a quick response and free flowing junction, the sensing bulband reference junction must not dry out.Short Term Storage (up to one week)Soak the electrode in ROSS pH electrode storage solution, Cat. No.810001, or standard pH electrode storage solution, Cat. No. 910001.For enhanced electrode performance, use ROSS pH electrode storagesolution.To minimize the chance of breakage, the 8220BNWP ROSS micro pHelectrode should be attached to an electrode stand and suspended ina beaker that contains storage solution. The electrode should not touchthe side or bottom of the beaker.Long Term Storage (more than one week)For refillable pH electrodes, fill the reference chamber and securelycover the fill hole. Cover the sensing bulb and reference junction withthe protective cap, sleeve or storage bottle containing a few drops ofstorage solution. Before returning the electrode to use, prepare it as anew electrode.Electrode Maintenance1. Inspect the electrode for scratches, cracks, salt crystalbuildup or membrane/junction deposits.2. Rinse off any salt buildup with distilled water. Remove anymembrane/junction deposits as directed in the General Cleaningsection.3. If a refillable electrode is used, drain the reference chamber, flushit with fresh ROSS fill solution and refill the chamber with freshROSS fill solution.Electrode Cleaning ProceduresGeneral CleaningWe offer a variety of Orion pH cleaning solutions for your convenience,including solutions for general cleaning, protein removal, bacteriaremoval, and oil and grease removal. See the list of Orion cleaningsolutions below the General Cleaning section of this manual. If youwould like to prepare your own cleaning solutions, the general cleaningprocedures contain several recommendations.12 ROSS Ultra, ROSS Ultra Triode and ROSS pH ElectrodesThermo Scientific Orion
1.a. For general cleaning, prepare a 0.1 M hydrochloric acid (HCl)or 0.1 M nitric acid (HNO3) solution.b. For cleaning oil or grease, prepare a 0.1 to 0.5 % liquiddetergent solution mixed with hot water.c. For cleaning bacterial contaminants, prepare a 1:10 dilution ofhousehold laundry bleach.2.a. Soak the electrode in the 0.1 M HCl or HNO3 for half an hourand stir t
Thermo ScientificThermo Scientific Orion ROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes 9 and water saturated with KCl. The ratio of methanol to water should be similar to the sample composition. For tips on measuring pH in non-aqueous samples, see Thermo Scientific Water Analysis Instruments Application Note 007.
tion (Thermo Orion 12.9 mS Conductivity Standard, Orion 011006), 0.01 molar KCl (Thermo Orion 1413 S Conductivity Standard, Orion 011007) or entry of the temperature-compensated conductivity of another known calibration solution. To protect the batteries, the meter switches off automati-cally when not operated for a longer time than specified.
PULSE ARC / MICRO TIG WELDERS Orion 200i Welder Orion 200 i Welding Power Supply, (welder, swing arm, microscope and all acc. come in one box) Orion 250s Arm Orion 250s Micro Pulse Arc Welder with Free-Floating Microscope Package (Orion Arm) (2 boxes) Orion 250s ADL Orion 250s Micro Pulse A
Thermo Scientific Orion AquaMate 8000 UV-Vis and AquaMate 7000 Vis spectrophotometers include a USB memory stick that contains over 260 preprogrammed methods for use with Orion AQUAfast, Merck and CHEMetrics reagent chemistries. Preprogrammed methods provide values for the test . Thermo Fisher Scientific .
10 Orion Versa Star Pro Meter User Manual Thermo Scientific Meter Connections 1. Connect a Thermo Scientific Orion Star series stirrer probe (Cat. No. 096019) to the STIRRER 1 and/or STIRRER 2 input on the meter. The stirrer probes are controlled and powered by the meter, eliminating the need for magnetic stir bars and plates.
Thermo Fisher Scientific Legal Entity Reference Guide Issue Date: April 1, 2014 Expiration Date: October 30, 2014 3 Introduction The Thermo Fisher Scientific Legal Entity Reference Guide is a map to the legal entities within Thermo Fisher. It presents two views of our company information: 1- A cross reference of former brands and product lines
Preface Safety and Special Notices ii Thermo Scientific BindIt Software for KingFisher Instruments User Manual Thermo Scientific Thermo Scientific KingFisher Presto User Manual (Cat. no. N17413) Thermo Scientific KingFisher 96 User Manual (Cat. no. 15018890) The Thermo Scientific BindIt Software user manual can be found in PDF format in the
Thermo ScientificTM Orion AQ3010 便携式浊度仪 Thermo ScientificTM Orion AQ3140 便携式 COD 测量仪 �䰳謋餑檯櫁 崬鎬胔 Orion COD㝵緒䰳獤㵸騩衊0-15000mg/L 僇圀婓栞蟭䰳斦紛讈穢 4饛7徠胾燪詭胾䰳楅傰崬㮅銁妭紛 17嚖枙悷5000瀐獤㵸 IP67䇝憠譀鈖
A programming manual is also available for each Arm Cortex version and can be used for MPU (memory protection unit) description: STM32 Cortex -M33 MCUs programming manual (PM0264) STM32F7 Series and STM32H7 Series Cortex -M7 processor programming manual (PM0253) STM32 Cortex -M4 MCUs and MPUs programming manual (PM0214)
