4-H Veterinary Science Genetics And Disease Lesson

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Veterinary SciencePreparatory Training for the Veterinary AssistantFloron C. Faries, Jr., DVM, MS

GeneticsFloron C. Faries, Jr., DVM, MS

Objectives Describe the structure of genetic material Describe the replication and expression ofgenetic material Discuss the role of biotechnology in theanimal industries Describe the process of selection forconvenience traits

A Science Genetics Study of inheritance (heredity) Inherited genetics How characteristics are passed from generation togeneration Study of genetic codes of body cells Molecular genetics Heredity Transmission of characteristics from parent to offspring By means of genes on chromosomes in nucleus ofbody cells Controlled by genes (DNA)

Chromosomes Occur in pairs One from paternal parentOne from maternal parent Made of DNA with a strand of 4 nucleotidebases

Genes (Traits) Occur in pairs on chromosome pairs One from each parent Stores information on chromosomes Tells cell how to build protein (good or bad) Proteins made are coded by specific genes Genetic code of nucleotide bases

Alleles Alternative copy of same gene Dominant or recessive Protein construction slightly different Function slightly different Different forms Co-dominant Co-recessive One dominant and one recessive Recessive genes expressed if no dominant genes May be good or bad

Genetic Code Combination and order of nucleotide bases In DNA of chromosomes in nucleus of bodycells Ordered by genes to build proteins

Nucleotide Bases Found in DNA of chromosomes Bind on pairs of chromosome strands Building blocks of genetic codeDNADNAAdenine --- ThymineThymine --- AdenineCytosine --- GuanineGuanine --- Cytosine

Transcription To make protein from nucleotide code of RNAcopy of chromosome DNA RNA strand copy binds with DNA strandMessenger RNA (mRNA) Leaves nucleusShuttles between nucleus and cytoplasma of cellDNARNAAdenine --- UracilThymine --- AdenineCytosine --- GuanineGuanine --- Cytosine

Translation Process of protein synthesis from the code onmRNA Ribosome in cytoplasma bind to mRNA toread code Produces strand of amino acids protein Code of 3 nucleotide bases codon

The start codon is AUG. Methionine is the only aminoacid specified by just one codon, AUG. The stop codons are UAA, UAG, and UGA. Theyencode no amino acid. The ribosome pauses and fallsoff the mRNA.

Body Cells Divide Mitosis – 2 cells with copies of chromosomepairs (diploid) Meiosis- 4 cells with copies of chromosomesingles (haploid)

Reproduction Transfer of haploids from both parents’(gametes) to form embryo (diploid) Phenotype A particular trait that is observed Genotype Genetic makeup of a single traitNot visible

Expression of Genes (Phenotype) Homozygous Genes are alike Dominant alleles PP Recessive alleles pp Heterozygous Genes are different One dominant and one recessive alleles Pp Carriers

AA x AABlack x BlackSireAAAA AA ABlackBlackDamAAAABlackABlackAll black

AA x AAAAAAAAAAAAAA

AA x aaBlack x RedSireAaAA aA aBlackBlackDamaAAaBlackaBlackAll black

AA x aaaaAAAaAaAaAa

Aa x AaBlack x BlackSireAAaA AA aBlackBlackDamaAaaBlackRed¾ Black¼ Reda

Aa x AaAaAaAAAaAaaa

Aa x aaBlack x RedSireAaaA aa aBlackRedDamaAaaBlackRed½ Black½ Reda

Aa x aaaaAaAaAaaaaa

Biotechnology A science- study of techniques Genetic Engineering (Biotechnology) Gene mappingRecombinant DNA processes – producesvaccines, hormones, testsGene deletion – produces vaccinesGene transfer – produces improved productionand resistanceNuclear transfer – produces cloning (twinning)

Transcription To make protein from nucleotide code of RNA copy of chromosome DNA RNA strand copy binds with DNA strand Messenger RNA (mRNA) Leaves nucleus Shuttles between nucleus and cytoplasma of cell

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