Research Safety And Training Manual - Veterans Affairs
Research Safety and Training Manual(Safety Plan)VA Hospital, Madison, WI (607)Research Service IHPPER&DRARCUW OBSWOCanimal biosafety levelAdministrative Officer for ResearchAnimal Research CommitteeAnimal Research FacilityBiosafety in Microbiological and Biomedical Laboratoriesbiosafety levelCenters for Disease ControlGreen Environmental Management SystemHazardous MaterialsMaterial Safety Data SheetsNational Institutes of HealthPersonal protective equipmentResearch and DevelopmentResearch Animal Resources Center (UW)University of Wis. Office of Biological Safetywithout compensation employeeModified from Research & Development Service VA Medical Center,Sioux Falls, SD) prepared by David A. Maddox, Ph.D.,Coordinator/R&D with assistance of Karen Munger, Ph.D., ResearchSafety Coordinator. Special thanks to Steve Breese, Gerene Denning, andSandi Rowe (Research Service, Iowa City VA) for permission to use theirmanual as a template for portions of this manual.Draft September 20101
Contents2,34ContentsTablesDraft September, 20102
I.II.III.EMERGENCY PLANA.The Research Disaster PlanB. Emergency Preparedness and Incident Response Plan1. Contact informationTable 1- Emergency Phone NumbersTable 2- Emergency Contact Numbers for ARF Disasters/Utility FailureTable 3- Members of the Subcommittee on Research Safety2. Fire/Explosion3. Tornado Procedure4. Personal Injury or Serious Illnessa)Severe Injury or Illnessb)Chemical Burnsc)Thermal Burnsd)Acute Inhalatione)Animal Bites5. Biological Spills6. Chemical Spills7. Radioactivity Spills8. Police9. Animal Evacuation10. Utility Failure Plana)Utility Failure in the Animal Research Facilityb)Electrical Failurec)Steam Failured)Communication Equipment Failuree)Water Failuref)Heating/Ventilation/Air Conditioning (HVAC) Failureg)VA Police supportRESEARCH SAFETY PROGRAMA. Subcommittee on Research SafetyB. Principal InvestigatorGENERAL LAB SAFETYA. Working in the Laboratory1. Non-emergency Injuries2. Housekeeping & ApparelB.Security PlanC. CentrifugesD. RefrigeratorsDraft September, 16161718192020
E.Glassware & Needles1. Glassware2. NeedlesF.Mouth PipettingG. Emergency ShowersH. Ultraviolet LightI.Biological safety cabinets, fume hoods, and laminar flow hoodsIV.CHEMICALSV.GAS CYLINDERSA. HandlingB. Identification & ProtectionC. Liquid NitrogenVI. BIOSAFETY PLAN (Biosafety Manual)A. Universal PrecautionsB. Using Toxic/Hazardous Substances/infectious agents/recombinant DNAC. Shipping Biological MaterialsVII. LABORATORY TRAININGVIII. TRAINING FOR USE OF ANIMALS IN RESEARCHIX.TRAINING FOR NON-RESEARCH PERSONNELDraft September, 201021212121222222222222232527283033354
TablesTable 1Emergency Phone Numbers5Table 2Emergency Contact Numbers for Utility Failure in the ARF6Table 3Subcommittee on Research Safety7Table 4SUMMARY OF RECOMMENDED BIOSAFETY LEVELS24Table 5SUMMARY OF RECOMMENDED BIOSAFETY LEVELS FORACTIVITIES IN WHICH EXPERIMENTALLY OR NATURALLYINFECTED VERTEBRATE ANIMALS ARE USED25Draft September, 20105
I. Emergency PlanA. The Research Disaster PlanThe Madison VA HOSPITAL has a facility-wide call-back plan in the event of anexternal disaster. The Administrative Officer (AO) for Research and the Call Center maintain alist of the telephone numbers of R&D employees (including WOCs) to be contacted in the event ofa disaster. All full-time research employees are responsible for reporting to the Hospital whenrequested. They should report to the Research Office (C-3127) and await directions from the AOfor Research.See also the Disaster Plan for the Animal Research Facility:Animal Research Committee Policy #09B. Emergency Preparedness and Incident Response PlanSee Also: Hospital Memo 001S-3Hospital Emergency Operations Plan (Policy 001S-3) andHospital Continuity of Operations Plan (COOP)1. Contact InformationEmergency Phone NumbersFIRE – Code Red.911MEDICAL – Code Blue/Rapid Response .911BEHAVIORAL – Code Orange .911MISSING PATIENT – Code White .911MISSING CHILD – Code Amber/Adam .911INCIDENT INVOLVING ACTIVE SHOOTER – Code Black .911BOMB THREAT .911VA POLICE .17270VA ENGINEERING .17042VA EMERGENCY/SAFETY MANAGER . 17383/17386VA CELL PHONE EMERGENCY CALL-IN NUMBER.13911NON-HAZARDOUS SPILLS .17045X.SMALL HAZARDOUS SPILLS 13422XI.LARGE HAZARDOUS SPILLS 9-911UTILITY/MEDICAL EQUIPMENT FAILURE .11811COMPUTERS FAILURES & SECURITY BREACHES .44357MADISON POLICE – NON EMERGENCY9-266-4275Employee HealthDraft September 2010172716
Table 2- Emergency Contact Numbers for ARF Disasters/Utility FailureNameTitleemailPhoneRobin FaustARF SupervisorRobin.Faust@va.gov256-1901ext. 17875Dr. RobertTaylorARF VeterinaryConsultantRtaylor atmatcmadison.edu246-6078Marvin RuppAdministrative Officerfor ResearchMarvin.rupp@va.gov280-7222Beverly BirdsallCoordinator for R&DBEVERLY.BIRDSALLat va.gov(608) 2561901 ext17007Draft September 2010AlternatephoneAfter hours,contact VAoperator at256-1901After hours,contact VAoperator at256-1901contact VAoperator@ 256-1901contact VAoperator@ 256-19017
Table 3: Research & Development Service Subcommittee on Research SafetyNameMembershipRoleChair/ScientistUW-M fessordra at medicine.wisc.edu263-1545ARFSupervisorRobin.Faust at archBiologistJamie.Swanlund at va.govExt.11405Tami LTishlerChemicalHygieneOfficerGEMSTami.Tishler at va.govDianeMerandaEOC LiaisonRadiationSafetyOfficerDiane.Meranda at va.gov256-1901Ext17427280-7014RandyWolff, PhDResearchSafety OfficerIACUC .gov 256-1901Ext17802RobStriker,MDIRB LiaisonR&D LiaisonAssistantProfessorRtstriker at wisc.edu2632994263-1545ToddEndresAlternate ForT.T. and D.M.Todd.endres2 at itySafetyOfficerComplianceOfficerJohn.Hudson3 at gAO ResearchMarvin.rupp at va.gov280-7222DavidAndes,MDRobinFaustDraft September 2010AlternatephoneContactVAoperator@ 2561901ContactVAoperator256-1901256-1901Ext 17386VAoperator@ 2561901Ext 17007or contactVAoperatorVAoperator@ 25619018
2. Fire/Explosion(See also Hospital memo 001S-2 SAFETY AND FIRE PROTECTION PLAN)FIRE PLANR-A-C-E1. Rescue: Remove patients/visitors from room/area of the fire as quickly as possible. Close door behind you.2. Alarm:a.b.c.3.Confine:a.b.c.4.Initiate alarm by activating nearest pull station.Call switchboard at extension 911 and give your name and the fire location.When alarm sounds, the Madison Fire Department responds automatically.After evacuating the patient/visitor, close room doors.Close all corridor doors in patient areas. Corridor doors provide 20 minutes of fire/smoke resistancewhen latched.Smoke and fire barrier doors close automatically when the fire alarm system is activated. Check tomake sure they have closed: if not, close them. DO NOT store items in front of these doors that wouldprevent them from closing.Evacuate/Extinguish:a.b.c.d.The first route of evacuation is horizontal, past the smoke or fire doors to a smoke free area.In a fully engaged fire, vertical evacuation, including the building. May be required if ordered by theDirector, Safety Manager, or Madison Fire Department.Use fire extinguishers only on small fires AFTER steps 1-3 have been completed.To use extinguishers: Break seal and pull the pin and aim at the base of the fire from 6-10 feet.Squeeze lever until fire is out.3. Tornado ProcedureDuring severe weather, tornadoes can occur quickly. Listen for the following overheadannouncements or radio announcements:1. Tornado Watch – Conditions are favorable for a tornado.Return to your work area.Review your tornado procedures.Prepare to move patients to safety.2. Tornado Warning – Funnel cloud sighted locally.Take immediate action to protect patients, visitors, and staff.*** DURING A TORNADO WARNING ***1. The Call Center initiates the plan after warning from the weather radio or sirens. Theoverhead announcement and Lynx Pop-Up message is:―Tornado Warning – Take Action Immediately‖2. Move ambulatory patients into the corridor and close the doors. All patients should be onthe same side of the hallway to maintain access.3. Protect non-ambulatory patients in their rooms by closing blinds and privacy curtains, andcovering the patients with blankets.4. Staff and visitors should take cover in corridors or away from windows.Draft September 20109
5. Areas without windows are not affected.6. Wait for the Call Center to announce ―All Clear.‖NOTE: Elevators may be used 4. Personal Injury or Serious Illness(See also Hospital memo 001S-2 SAFETY AND FIRE PROTECTION PLAN)PATIENT(S): Patient injuries should be treated by the Emergency Dept physician. Assistpatient in getting treatment. Within two days, track incident by completing Form 10-2633 andcontact the Patient Safety Officer at 17742.VISITOR(S): Go to Emergency Dept for immediate evaluation and treatment if necessary.VA employee should assist visitor to Emergency Dept, and report the incident to the SafetyManager at 17386.a. Severe Injury or IllnessInjuries that involve broken bones, excessive bleeding, unconsciousness,extensive burns, or serious illness suggesting heart attack, stroke, shock, et cetera, should bereported by dialing the emergency telephone number 911. Be sure to provide the location of theperson in need of assistance to the emergency personnel. If possible, send someone to meet anddirect the response team to the location. If serious bleeding occurs, use direct pressure on thewound with a gauze pad, towel, et cetera, until medical assistance arrives. Do NOT applytourniquets.b. Chemical BurnsIf a chemical burn occurs to the eye or skin, get the person to a Safety Shower orEye Wash as quickly as possible and flush with large amounts of cool water to rinse away thechemical. If the chemical penetrates the clothing, promptly remove the clothing and flush theskin with water. Call Employee Health Nurse (x17271) or call the Emergency Department (608280-7066, 24 hr triage line) and/or transport the victim to Emergency Department in the mainhospital. It is best to have someone call ahead to alert the Emergency Department that you arecoming. Bring along the MSDS or any other available information available about thechemical.In order to have the MSDS available during an emergency, make sure a short cutto one of the MSDS databases is available on your Desktop. Having paper copies of MSDSs onhand has the benefit of not requiring electricity or internet connection at the time of theaccident.For VA networked computers: http://vaww.ceosh.med.va.gov/ceosh/MSDS.shtmlFor UW networked computers: http://hazard.com/msds/index.phpDraft September 201010
c. Thermal BurnsThermal burns such as those caused by hot plates or flames should be immersedin ice-cold water or an ice pack placed over the burned area, but AVOID the use of ointments.Continue to cool the burn and Call Employee Health Nurse (x17271) or call the EmergencyDepartment (608-280-7066, 24 hr triage line) and/or transport the victim to EmergencyDepartment in the main hospital. It is best to have someone call ahead to alert the EmergencyDepartment that you are coming.d. Acute InhalationInhalation of gas, fumes, dust, et cetera can cause severe illness, unconsciousness, or even death.Some examples are chlorine, hydrogen sulfide, carbon monoxide, hydrogen cyanide, andhydrochloric acid. Gases such as nitrogen and carbon dioxide (from dry ice) are not corrosiveor toxic but are injurious due to the lack of oxygen. First aid in all such circumstancesrequires the quick removal of the victim from the affected area. If the victim is breathing, CallEmployee Health Nurse (x17271) or call the Emergency Department (608-280-7066, 24 hrtriage line) and/or transport the victim to Emergency Department in the main hospital. It is bestto have someone call ahead to alert the Emergency Department that you are coming. If thevictim has stopped breathing, call 911 (Code Blue) and begin CPR until help arrives.e. Animal BitesThe possibility of animal bites is a legitimate concern for those labs working with rats andrabbits. In the event of a bite, flush the wound with soap and water and report to EmployeeHealth, call Employee Health Nurse (x17271) or call the Emergency Department (608-2807066, 24 hr triage line) for treatment and consultation. The event must be reported to the person‘semployer. Rodent bites do not pose a rabies threat, but this type of wound can be severe, with thepotential for infection and sepsis. Bitten employees should report to Employee Health fortreatment of the wound and a prophylactic tetanus shot if it has been more than 5 years sincetheir last DPT immunization.5. Sample Protocols for Containing Biohazardous he following sample protocols are provided to facilitate emergency planning and should be modified to cover thepotentially biohazardous materials used in each laboratory. These instructions should be displayed in thelaboratory and periodically reviewed with personnel. Download PDF copyBIOHAZARDOUS SPILLS - OUTSIDE Containment (Biological SafetyCabinet) Alert co-workers and leave lab area immediately.Close door, post lab with "Do Not Enter" sign.Remove and put contaminated garments into a container for autoclaving.Wash hands/face with soap/antimicrobial agent.Notify PI/supervisor.Wait at least 30 minutes before re-entry (to allow aerosols to dissipate).Wear PPE upon re-entry (disposable gown, mask/eye protection, doublegloves, etc.).Cover spill with disinfectant soaked paper towels.Draft September 201011
Carefully pour an appropriate disinfectant solution (1:10 dilution ofhousehold bleach) around spill, taking care not to create aerosols whilepouring.Let stand for at least 20 minutes.Transfer all contaminated materials into an autoclave bag.Wash and mop entire area with appropriate disinfectant.Remove & discard PPE. Autoclave.Shower or wash hands with soap/antimicrobial agent.Report incident to the BSO (Biological Safety Officer), Occupational Health andthe PI (if not already contacted).BIOHAZARDOUS SPILLS - INSIDE Containment (Biological SafetyCabinet) Immediately stop all work, but leave BSC blower fan on during clean-up.Wear PPE (gloves, lab coat, eye protection).Cover spill with disinfectant soaked paper towels and pour an appropriatedisinfectant solution around spill.Using paper towels and appropriate disinfectant detergent, wipe down walls,work surfaces and equipment.Flood work surface and drain pan (Type II BSC) with disinfectant. Let stand forat least 20 minutes.Wipe up all excess disinfectant.Autoclave all contaminated materials.Report incident to the PI (if not already contacted).6. Chemical Spills Report non-hazardous spills and accidental exposures to Environmental Support Service at13422 and the Industrial Hygienist at 17749. Report large hazardous spills to the Madison Fire Department HAZMAT Team at 9-911. ( 5gallons)POLICY: The hospital will safely manage chemical use from point of purchase to final disposal.RIGHT TO KNOW: Every employee has the right to know the health hazards associated withany chemicals he/she works with.SUPERVISORS:1. Make Material Safety Data Sheets (MSDS) for each chemical in your area available to youremployees upon request. Contact IT if a MSDS online icon is not located on your Desktop.2. Label all containers containing hazardous chemicals.3. Train employees in the safe handling of chemicals.4. Provide appropriate personal protective equipment.EMPLOYEES:1. Consult MSDS to determine hazards of chemicals in use.2. Follow all safety instructions and use of protective equipment.3. Follow service spill plan for cleanup of minor chemical spill.4. Report all spills as noted above, in absence of supervisor.Draft September 201012
DISPOSAL: Questions regarding disposal of hazardous materials and waste should be directedto the GEMS Coordinator at 608-256-1901 ext 17427.7. Radioactivity SpillsFrom Hospital Memorandum No. 001S-09-13 ―In case of a spill or other accident,alert nearby personnel to call the RSO. Confine spill, block off and mark area,decontaminate, and monitor before moving temporary sign or barricade. The locationof the nearest Radiation Material Spill Kit should be listed on the emergencyinformation sheet posted on the door of every laboratory authorized to use radiation.‖Report radioactivity spills and accidental exposures to the Radiation Safety Officer atx17014, (or the Safety Manager at x17386) and Environmental Support Service atx170458. PoliceHospital police officers should be notified in an emergency situation because theyhave radios, keys, and quick access to emergency aid. For all Emergencies dial 911.For non-emergencies dial ext.17270 .8. Animal EvacuationIn the case of an immediate life-threatening disaster, call in an alarm (911), evacuatepeople, wait outside for the Fire Department (in case of fire) or seek shelter as appropriate. Donot evacuate animals.If there is not an immediate threat to human life, but the situation poses a potential threat(e.g., approaching toxic fumes, fire in the main hospital, possibility of smoke/other damagespreading to the Animal Research Facility (ARF), or in the event of utility failure) seekauthorization from the individual in charge- ARF Supervisor, Research AO, Fire Chief,Coordinator/R&D, - to evacuate the animals as follows:a. to appropriate housing in labs, and if necessary outbuildings (downstairs Building2, Laundry), with notification to ARF Supervisor at this time, and/orb. to other Animal Care facilities- call ARF Supervisor extension 17875 (or if theARF Supervisor is not available the Research Office (extension 17007) make thenecessary arrangements at this time (after hours, See Table 2 above for contactinformation).If the situation poses no threat to human life, remain with relocated animals; otherwise,be advised by the ARF Supervisor. Following the disaster, as specified by the ARF Supervisoror his/her designated representative, return animals to the ARF, have the animals remain at thelocation to which they were moved, or move them to a new location; and continue care.Draft September 201013
9. Utility Failure PlanIf a utility failure occurs during normal administrative working hours (Mon-Friday, 8:00AM - 4:30 PM), notify the Research Office (17007). Research Office personnel will notifyEngineering Service if necessary. Utility failure at any other time should be reported to theEngineering Service (17042 or 11811). Please contact Principal Investigator on Lab EmergencyInformation Data sheet as identified outside lab door.If the utility failure creates a hazardous situation, immediate action to secure the area willbe taken by the individuals in the area prior to contacting the Research Office. Upon notifyingthe Research Office, the individual will report the nature and extent of the hazardous situation.The Research Office will relay this information to the Research Safety Officer (see Table 3) andEngineering Service.a. Utility Failure in the Animal Research FacilityIn the event of a utility failure in the Animal Research Facility (ARF), the ARFSupervisor, the Administrative Officer for Research, and the C
Research Safety and Training Manual (Safety Plan) VA Hospital, Madison, WI (607) Research Service (151) Acronyms ABSL animal biosafety level AO Administrative Officer for Research ARC Animal Research Committee ARF Animal Research Facility BMBL Biosafety in Microbiological and Biomedical Laboratories BSL biosafety level
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