AssayMAP Bravo Platform Installation Guide

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AssayMAP Bravo PlatformInstallation GuideOriginal Instructions

NoticesManual Part NumberWarrantySD-V1000175 Revision DThe material contained in this document isprovided “as is,” and is subject to beingchanged, without notice, in future editions.Further, to the maximum extent permittedby applicable law, Agilent disclaims all warranties, either express or implied, withregard to this manual and any informationcontained herein, including but not limitedto the implied warranties of merchantabilityand fitness for a particular purpose. Agilentshall not be liable for errors or for incidentalor consequential damages in connectionwith the furnishing, use, or performance ofthis document or of any information contained herein. Should Agilent and the userhave a separate written agreement withwarranty terms covering the material in thisdocument that conflict with these terms,the warranty terms in the separate agreement shall control.November 2019Copyright Agilent Technologies, Inc. 2019No part of this manual may be reproducedin any form or by any means (includingelectronic storage and retrieval ortranslation into a foreign language) withoutprior agreement and written consent fromAgilent Technologies, Inc. as governed byUnited States and international copyrightlaws.Contact InformationAgilent Technologies Inc.Automation Solutions5301 Stevens Creek Blvd.Santa Clara, CA 95051USAWeb:https://www.agilent.comContact mentation wledgementsMicrosoft and Windows are eitherregistered trademarks or trademarks of theMicrosoft Corporation in the United Statesand other countries.Technology LicensesThe hardware and/or software described inthis document are furnished under a licenseand may be used or copied only inaccordance with the terms of such license.Restricted Rights LegendIf software is for use in the performance ofa U.S. Government prime contract orsubcontract, Software is delivered andlicensed as “Commercial computersoftware” as defined in DFAR 252.227-7014(June 1995), or as a “commercial item” asdefined in FAR 2.101(a) or as “Restrictedcomputer software” as defined in FAR52.227-19 (June 1987) or any equivalentagency regulation or contract clause. Use,duplication or disclosure of Software issubject to Agilent Technologies’ standardcommercial license terms, and non-DODDepartments and Agencies of the U.S.Government will receive no greater thanRestricted Rights as defined in FAR 52.22719(c)(1-2) (June 1987). U.S. Governmentusers will receive no greater than LimitedRights as defined in FAR 52.227-14(June1987) or DFAR 252.227-7015 (b)(2)(November 1995), as applicable in anytechnical data.Safety NoticesWARN I N GA WARNING notice denotes a hazard. It calls attention to an operatingprocedure, practice, or the like that, ifnot correctly performed or adheredto, could result in personal injury ordeath. Do not proceed beyond aWARNING notice until the indicatedconditions are fully understood andmet.CAUT IONA CAUTION notice denotes a hazard.It calls attention to an operating procedure, practice, or the like that, if notcorrectly performed or adhered to,could result in damage to the productor loss of important data. Do not proceed beyond a CAUTION notice untilthe indicated conditions are fullyunderstood and met.

Contents1. Before you start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .About this guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Related guides and where to find them . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12242. Installing the platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Overview of the hardware components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Computer requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Installation workflow for standard configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Installing the Bravo risers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Before you install the AssayMAP head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Installing the Wash Station and Pump Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Installing the standard platepads. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Installing the Peltier Thermal Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26Installing and connecting the Orbital Shaking Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313. Setting up the computer and software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Setting up the computer and installing the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Turning on the platform and setting up the VWorks software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Verifying the profile settings and configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .About verifying the deck configuration in the profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Verifying the Autofill Station configuration (wash station) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Verifying the Peltier Thermal Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Verifying the Orbital Shaking Station configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Initializing the profile that you are verifying . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Testing the accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .353641434749515355564. Setting the teachpoints. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Teaching part 1: Setting teachpoints in AssayMAP 1 profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Teaching part 2: Adjusting teachpoints for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Teaching part 3: Updating other profiles based on AssayMAP 1. . . . . . . . . . . . . . . . . . . . . . . . . . . .Checking and adjusting the gripper offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61626773775. Verifying the installation and setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Verification workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .About installing the Bravo pendant, Light Curtain, and shields . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Opening the Workbench and accessing the utilities. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Running the Startup utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Testing the safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Washing the syringes to prepare for first time use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Performing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8384848687899193AssayMAP Bravo Platform Installation Guideiii

ContentsRunning the Syringe Test utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103Running the Shutdown utility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104A. Manually updating teachpoints in each profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105Workflow for setting teachpoints in each profile manually. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106Adjusting the teachpoint for the Bravo Plate Riser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107Adjusting the teachpoint for the red PCR plate insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109ivAssayMAP Bravo Platform Installation Guide

1 Before you start “About this guide” on page 2 “Related guides and where to find them” on page 2 “Service documentation” on page 4 “Safety information” on page 41

1 Before you startAbout this guideAbout this guideIntentThis installation guide is intended for use by qualified and authorized AgilentTechnologies service personnel who possess an in-depth service background and havereceived training in the installation of the AssayMAP Bravo Platform.What this guide coversThis guide describes the following software versions, which are required for theAssayMAP Bravo Platform: Protein Sample Prep Workbench 3.2 VWorks Automation Control 13.1.5Microsoft Excel 2016, which is not included, is required for the reagent volumecalculators and method setup tools in the Protein Sample Prep Workbench.What is new in this revisionandFeature and descriptionSee Added a caution that the 25 µL cartridgesrequire a wash station with wide-borechimneys.“Overview of the hardwarecomponents” on page 6Updated the software requirements.“Installing the software” on page 40Revised the verification procedures to reflectchanges in Protein Sample Prep Workbenchv3.2.“Verifying the installation andsetup” on page 83Related guides and where to find themChecklistsYou can find the following checklists on the Agilent AssayMAP Bravo Platform productpage at atform2 Site Preparation Checklist. Specifies the site requirements that must be met beforeinstalling the AssayMAP Bravo Platform. Installation Checklist. Lists the tasks to be performed to install the AssayMAP BravoPlatform.AssayMAP Bravo Platform Installation Guide

1 Before you startRelated guides and where to find themUser guidesPrinted copies of the following guides are provided with the platform.GuideDescriptionBravo Platform Unpacking GuideUnpacking instructions for the Bravo PlatformBravo Platform Safety and Installation GuideSafety guidelines, site specifications, and installationproceduresGeneral Safety GuideSafety label descriptions and guidelines for Agilent AutomationSolutions products.The following guides are available as links in the Literature Library page of the ProteinSample Prep Workbench and available in the online VWorks Knowledge Base.GuideDescriptionBravo Platform User GuideSetting up, operating, maintaining, and troubleshooting theBravo PlatformAssayMAP Bravo Platform Error RecoveryGuideError messages, and how to handle themAssayMAP Bravo Platform LabwareReference GuideLabware photo reference and descriptionsAssayMAP Syringe Test Utility User GuideRunning a syringe test of the Bravo 96AM HeadAssayMAP Bravo Syringe Replacement UserGuideReplacing a defective syringe in the Bravo 96AM Head96 Channel Wash Station MaintenanceGuideReplacing chimneys and troubleshooting problems in a 96channel wash station.VWorks Knowledge BaseCreating and editing VWorks protocols and applicationinterfacesTo access the VWorks Knowledge Base, do one of the following: From within VWorks software, select Help Knowledge Base or press F1. From the Windows desktop, select StartVWorks Knowledge Base. Go to the online VWorks Knowledge Base at www.agilent.com/chem/askb andclick the link for PDF user guides. Agilent Technologies To access the context-sensitive help feature in the VWorks software:1In the main window of the VWorks software, click the help button. The pointerchanges to. Notice that the different icons or areas are highlighted as youmove the pointer over them.2Click an icon or area of interest. The relevant topic or document opens.AssayMAP Bravo Platform Installation Guide3

1 Before you startSafety informationService documentationAgilent field service engineers can find instructions on how to maintain and repair theBravo Platform in the following guides: Bravo Platform Service Guide Pipette Head Service GuideChanges to existing procedures and additional service procedures are published asservice bulletins and posted in the Support Portal support/default.aspxSafety informationBefore installing and using the Bravo Platform, make sure you are aware of the potentialhazards and understand how to avoid being exposed to them. You must be properlytrained in the correct and safe installation and operation of the device. For details, seethe Bravo Platform Safety and Installation Guide.Do not substitute parts or make any unauthorized modifications to the Bravo Platformor the supplied safety equipment.WAR NING4Changing or modifying the Bravo Platform safety equipment may prevent the safeoperation of the device, invalidate its safety compliance, and lead to personal injuryor property damage. Any customer who does not use the supplied safety equipmentor who modifies the supplied safety equipment assumes full responsibility forproviding an appropriate level of safety for its operators and for providing theapplicable safety compliance marking and documentation.AssayMAP Bravo Platform Installation Guide

2 Installing the platform “Overview of the hardware components” on page 6 “Computer requirements” on page 7 “Installation workflow for standard configuration” on page 8 “Installing the Bravo risers” on page 9 “Before you install the AssayMAP head” on page 12 “Installing the Wash Station and Pump Module” on page 15 “Installing the standard platepads” on page 25 “Installing the Peltier Thermal Station” on page 26 “Installing and connecting the Orbital Shaking Station” on page 315

2 Installing the platformOverview of the hardware componentsOverview of the hardware componentsThe following figure and table show the components of the AssayMAP Bravo Platform,which are required for running the AssayMAP protocols.Figure AssayMAP Bravo Platform componentsItemC AU T I O N6Required hardware1Gripper upgrade2Bravo 96AM Head396AM Wash Station or the later model 96 Channel Wash Station4Pump Module 2.0 and two carboys596AM Cartridge & Tip Seating Station6Risers, 146 mm7STC controller8Peltier Thermal Station with custom plate nest9Thermal plate insert10Orbital Shaking Station with Control UnitTo avoid a hardware crash and equipment damage, ensure that the wash stationcontains the white wide-bore chimneys when using the 25 µL cartridges.AssayMAP Bravo Platform Installation Guide

2 Installing the platformComputer requirementsNote: The white wide-bore chimneys work for both 5-µL and 25-µL cartridges and arestandard on wash stations purchased in 2020 onward. The wide-bore chimneys arewhite plastic, whereas the standard-bore chimneys are a semi-clear plastic. For details,see the 96 Channel Wash Station Maintenance Guide.In addition to the components listed in the preceding table, the AssayMAP BravoPlatform includes: Bravo Standard Light Curtain and the emergency-stop pendant shown in thefollowing figures Computer workstationFigure Standard configuration with Light Curtain66.5 cm84.3 cm52.5 cmFigure Emergency-stop pendantComputer requirementsThe software user interface for the AssayMAP applications is optimized for a monitorwith a resolution of 1920 x 1080 (full HD). For a full list of the computer requirements,see the software release notes.AssayMAP Bravo Platform Installation Guide7

2 Installing the platformInstallation workflow for standard configurationInstallation workflow for standard configurationStep1For this task.See.Verify that the installation site meets the site requirements.Bravo Platform Safety andInstallation GuideIMPORTANT Ensure that the laboratory table is not bowedand is perfectly flat and rigid. A bowed or wobbly table willcause placement errors during protocol execution.Clear the table of all other material that may interfere with theinstallation.2Unpack and connect the computer.Manufacturer’s instructions3Unpack the Bravo Platform and accessories.Bravo Platform Unpacking GuideIMPORTANT Do not remove the y-axis shipping stop fromthe head mount until after you have installed the risers(“Installing the Bravo risers” on page 9).84Install the Bravo deck risers.“Installing the Bravo risers” onpage 95Connect the Bravo Platform power cord, communicationscable, and pendant cable.Bravo Platform Safety andInstallation Guide6Install the Bravo 96AM Head.“Before you install the AssayMAPhead” on page 127Install the 96AM Wash Station and Pump Module.“Installing the Wash Station andPump Module” on page 158Install the standard platepads at the specified Bravo decklocations.“Installing the standard platepads”on page 259Install the Peltier Thermal Station and the Inheco STCController.“Installing the Peltier ThermalStation” on page 2610Install the Orbital Shaking Station.“Installing and connecting theOrbital Shaking Station” onpage 3111Set up the software, and configure the device files.“Setting up the computer andsoftware” on page 3512Set the AssayMAP head teachpoints in every one of theAssayMAP profiles. Verify the gripper offset in every profile.“Setting the teachpoints” onpage 6113Install the Bravo shields and Light Curtain.Bravo Platform Safety andInstallation Guide14Verify the installation and setup.“Verifying the installation andsetup” on page 83AssayMAP Bravo Platform Installation Guide

2 Installing the platformInstalling the Bravo risersInstalling the Bravo risersBefore you startWAR NINGC AU T I O NThe Bravo device weighs approximately 52.1 kg (114.9 lb). Attempting to move theBravo device without assistance could cause personal injury. Request assistanceand use proper lifting techniques when moving the Bravo device.Tugging on the tie bar or using it to lift the Bravo Platform can damage the device. Donot grab the tie bar when lifting the Bravo Platform.00233BravoProper lift00234BravoImproper lift00233IMPORTANT00234The following procedure is intended only for fully qualified and trained Agilent serviceengineers.Ensure that you have the following: Two people to lift the Bravo Platform Foam pad (3-inch thick) or similar cushioning material to use when you tilt theBravo Platform onto its back plateNote: EPE foam is preferred due to its firmness. Risers Hex driversProcedureC AU T I O NWhen tilting the Bravo Platform backwards to rest on its back plate, the head mountcan slide back rapidly along the y-axis and collide with the back plate. To preventpotential equipment damage, ensure that the y-axis shipping stop remains installedon the head mount until after you install the risers. If the shipping stop has alreadybeen removed during unpacking, ensure that the head mount is resti

2 AssayMAP Bravo Platform Installation Guide About this guide Intent This installation guide is intended for use by qualified and authorized Agilent Technologies service personnel who possess an in-depth service background and have received training in the installation of the AssayMAP Bravo Platform. What this guide covers

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