CHROMATOGRAPHY - Bharathidasan University
CHROMATOGRAPHYBiochemistry of Medics1
ChromatographyBiochemistry of Medics2
ChromatographyBiochemistry of Medics3
Chromatography- PrincipleBiochemistry of Medics4
Chromatography- ClassificationBiochemistry of Medics5
Chromatography- Classification(Contd.)Biochemistry of Medics6
Partition ChromatographyBiochemistry of Medics7
Paper chromatographyBiochemistry of Medics8
Paper chromatographyBiochemistry of Medics9
Paper chromatography- ProcedureBiochemistry of Medics10
Paper chromatography- ProcedureBiochemistry of Medics11
Ascending and Descending Paper ChromatographyBiochemistry of Medics12
Paper Chromatography -AnalysisBiochemistry of Medics13
Rƒ valueBiochemistry of Medics14
Two dimensional chromatographyBiochemistry of Medics15
Significance of Paper chromatographyBiochemistry of Medics16
Thin layer chromatography (TLC)Biochemistry of Medics17
TLC- ProcedureBiochemistry of Medics18
TLC -OverviewBiochemistry of Medics19
Significance of TLCBiochemistry of Medics20
Advantages of TLC over paper chromatographyBiochemistry of Medics21
2)Adsorption chromatographyBiochemistry of Medics22
2)Adsorption chromatography- ElutionBiochemistry of Medics23
Adsorption chromatographyThe substance loosely adsorbed to the stationary medium comes in the earlier fraction.Biochemistry of Medics24
Ion – Exchange chromatographyBiochemistry of Medics25
Ion – Exchange chromatographyBiochemistry of Medics26
Ion – Exchange chromatography- ProcedureBiochemistry of Medics27
Ion – Exchange chromatographyProcedure (Contd.)Biochemistry of Medics28
Types of ion exchanges resinsBiochemistry of Medics29
Ion – Exchange chromatographyAn OverviewBiochemistry of Medics30
4) Gel filtration chromatography orMolecular Sieve chromatographyBiochemistry of Medics31
Gel filtration chromatography orMolecular Sieve chromatographyBiochemistry of Medics32
Gel filtration chromatography-AnOverviewLarger particles come out first, while smaller particles come in later fractionsBiochemistry of Medics33
5) Affinity chromatographyBiochemistry of Medics34
Affinity chromatography- SignificanceBiochemistry of Medics35
Affinity chromatography- SignificanceBy using antibodies, antigens can be easily separated.Conversely, antibodies can be purified by passing through a columncontaining the antigen.Specific Antibody binds to a specific antigen attached to the stationary medium, theremaining antibodies come out in earlier fractions.Biochemistry of Medics36
6) High performance liquid chromatography-Biochemistry of Medics37
High performance liquid chromatography-Biochemistry of Medics38
High performance liquid chromatographySignificanceBiochemistry of Medics39
High performance liquidchromatography- ApparatusBiochemistry of Medics40
6) Gas liquid chromatography-Biochemistry of Medics41
Gas liquid chromatography-Biochemistry of Medics42
Gas liquid chromatography-ApparatusBiochemistry of Medics43
SummaryBiochemistry of Medics44
Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions Biochemistry of Medics 33. 5) Affinity chromatography . High performance liquid chromatography-Significance Biochemistry of Medics 39. High performance liquid chromatography- Apparatus Biochemistry of Medics 40.
2.3.1 Liquid chromatography (LC) 20 Size-exclusion chromatography (SEC) 20 Ion-exchange chromatography (IEC) 22 Reversed-phase chromatography (RP) 23 Reversed-phase ion pairing chromatography (RPIP) 24 2.3.2 Gas chromatography (GC) 25 2.3.3 Electrophoretic techniques 25 2.3.4 Isotope dilution analysis (IDA) 26 3 EXPERIMENTAL RESEARCH 27 Aims 27
Bharathidasan University, Trichy. Qualification x MPhil (English Literature) Bishop Heber College, (Autonomous), Affiliated to Bharathidasan University, Trichy. x MA (English Literature) - Bishop Heber College, (Autonomous), Affiliated to Bharathidasan University, Trichy. x BA (English Literature
Chromatography 481 27.4.2 Partition (Liquid–Liquid) Chromatography 482 27.4.2.1 Introduction 482 27.4.2.2 Coated Supports 483 27.4.2.3 Bonded Supports 483 27.4.3 Ion-Exchange Chromatography 483 27.4.4 Size-Exclusion Chromatography 485 27.4.5 Affinity Chromatography 488 27.5 Analys
1. A LC technique which separates solutes based on their adsorption to an un-derivatized solid particles is known as adsorption chromatography, or liquid-solid chromatography. 2. Adsorption chromatography was the first type of column liquid chromatography developed (Tsweet, 1903). However, it is currently
Affinity chromatography, (g) Gas chromatography, (h) Supercritical fluid chromatography, (i) High Performance Liquid Chromatography, (j) Capillary electrophoresis, 4. Classification of chromatographic methods - according to separation methods, according to development procedures. (i)Thin Layer Chromatography: Theory and principles, outline of the
Gas Chromatography Like other methods of chromatography, a partitioning of molecules must occur between the stationary phase and the mobile phases in order to achieve separation. This is the same equilibrium that is seen between the stationary phase and mobile phase in column chromatography or thin-layer chromatography. .
“Laboratory Chromatography Guide” – A close look at preparative liquid chromatography The present “Laboratory Chromatography Guide” is dedicated to preparative liquid chromatography, a common purification techni-que in most chemical or life science laboratories. T
Araling Panlipunan . 2 Araling Panlipunan 2 Ma. Ther Inilimbag sa Pilipinas ng _ Department of Eduction-Instructional Materials Council Secretariat (DepEd-IMCS) Office Address: nd 2 Floor Dorm G, PSC Complex Meralco Avenue, Pasig City Philippines 1600 Telefax: (02) 634-1054 or 634-1072 E-mail Address: imcsetd@yahoo.com Mga Bumuo ng Kagamitan ng Mag-aaral Consultant: Zenaida E. Espino .
