Principles And Techniques Of Biochemistry Molecular Biology

:. Revy. Courtesy ofience Photo library.k Image/Scienceurtesy of ScienceEvans. Courtesy ofPrinciples and Techniques ofBiochemistry andMolecular BiologySeventh editionEdited byKEITH WILSON AND JOHN WALKER.,.}:, . CAMBRIDGE:::UNIVERSITY PRESS

CONTENTSPreface to the seventh editionList of contributorsList of abbreviations · .page xixiiiXV1 Basic principlesK. WILSON1ues of Practicalues of Practical1.11.21.31.41.51.62 Cell culture techniques6-II. Walker,3616353738A.R. BAYDOUN 1iques of Biochemistryn,Biochemical and molecular biology studiesUnits of measurementWeak electrolytesQuantitative biochemical measurementsSafety in the laboratorySuggestions for further reading2.12.22.32.42.52.62. 72.82.9IntroductionThe cell culture laboratory and equipmentSafety considerations in cell cultureAseptic techniques and good cell culture practiceTypes of animal cell, characteristics and maintenance in cultureStem cell cultureBacterial cell culturePotential use of cell culturesSuggestions for further reading3 Centrifugation38394344496168717273K. OHLENDIECK3.13.23.33.43.53.6vIntroductionBasic principles of sedimentationTypes, care and safety aspects of centrifugesPreparative centrifugationAnalytical centrifugationSuggestions for further reading737479869599

c)IviContents4 Microscopy100S. W. PADDOCK4.14.24.34.44.54.64.74.8IntroductionThe light microscopeOptical sectioningImaging living cells and tissuesMeasuring cellular dynamicsThe electron microscope (EM)Image archivingSuggestions for further reading5 Molecular biology, bioinformatics and basic techniques100103116123126129133136138R. ductionStructure of nucleic acidsGenes and genome complexityLocation and packaging of nucleic acidsFunctions of nucleic acidsThe manipulation of nucleic acids - basic tools and techniquesIsolation and separation of nucleic acidsMolecular biology and bioinformaticsMolecular analysis of nucleic acid sequencesThe polymerase chain reaction (PCR)Nucleotide sequencing of DNASuggestions for further mmurImmurLateralEpitopImmmFlu oreCell a1ImmuImmuAntihTheraThe fiSuggc8 Protfand·J. Wf.8.1 Ionic8.2 Prot 8.3 Prott8.4 Prott8.5 Protc8.6 Sug 9 Ma5A. A6 Recombinant DNA and genetic analysis195R. ductionConstructing gene librariesCloning vectorsHybridisation and gene probesScreening gene librariesApplications of gene cloningExpression of foreign genesAnalysing genes and gene expressionAnalysing whole genomesPharmacogenomicsMolecular biotechnology and applicationsSuggestions for further reading7 lmmunochemical . BURNS7.1 Introduction7.2 Making asDetcStnAmCorSu 10J.10.1 G10.210.310.410.5 c10.6 Mi10.7 S't

127.1 37.147.15ContentsImmunoassay formatsImmuno microscopyLateral flow devicesEpitope mappingImmunoblottingFluorescent activated cell sorting (FACS)Cell and tissue staining techniquesImmunocapture polymerase chain reaction (PCR)Immunoaffmity chromatography (IAC)Antibody-based biosensorsTherapeutic antibodiesThe future uses of antibody technologySuggestions for further reading2832912912922932932942952952962972992998 Protein structure, purification, characterisationand function analysis300J. WALKER8.18.28.38.48.58.6Ionic properties of amino acids and proteinsProtein structureProtein purificationProtein structure determinationProteomics and protein functionSuggestions for further reading9 Mass spectrometric techniques300304307328340351352A. Mass analysersDetectorsStructural information by tandem mass spectrometryAnalysing protein complexesComputing and database analysisSuggestions for further reading10 Electrophoretic techniques352354359377379390394397399J. WALKER10.110.210.310.410.510.610.7General principlesSupport mediaElectrophoresis of proteinsElectrophoresis of nucleic acidsCapillary electrophoresisMicrochip electrophoresisSuggestions for further reading399403407422427431432

,.-cviiiContents11 Chromatographic techniquesContentsix43315 EnzymK. nciples of chromatographyChromatographic performance parametersHigh-performance liquid chromatographyAdsorption chromatographyPartition chromatographyIon-exchange chromatographyMolecular (size) exclusion chromatographyAffinity chromatographyGas chromatographySuggestions for further reading12 Spectroscopic techniques: I Spectrophotometric techniquesK. WILS433435446453455459462465470476477A. nUltraviolet and visible light spectroscopyFluorescence spectroscopyLuminometryCircular dichroism spectroscopyLight scatteringAtomic spectroscopySuggestions for further reading13 Spectroscopic techniques: II Structure and 315.415.515.6Charac1EnzymAnalytEnzymContraSugge16 PrinciJ. FY FI16.116.216.316.416.5PrinciClinicExamSugg Ackn117 Cell1K. WI17.117.217.317.417.517.6ReceJQuanLiga1MedReceA. nInfrared and Raman spectroscopySurface plasmon resonanceElectron paramagnetic resonanceNuclear magnetic resonanceX-ray diffractionSmall-angle scatteringSuggestions for further reading14 Radioisotope techniques522523527530536546549551553R.J. SLATER14.114.214.314.414.514.6Why use a radioisotope?The nature of radioactivityDetection and measurement of radioactivityOther practical aspects of counting of radioactivity and analysis of dataSafety aspectsSuggestions for further reading5535545615735775801818.118.2 D18.318.4IndTh1

2 Cell culture techniques A.R. BAYDOUN 2.1 Introduction 2.2 The cell culture laboratory and equipment 2.3 Safety considerations in cell culture 2.4 Aseptic techniques and good cell culture practice 2.5 Types of animal cell, characteristics and maintenance in culture 2.6 Stem cell culture 2. 7 Bacterial cell culture