ANIMAL HEALTH CENTRE Services And Fee Schedule - British Columbia

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ANIMAL HEALTH CENTREServices and Fee ScheduleFebruary 2021

Table of ContentsGeneral Information . 1Animal Health Centre . 1Mandate . 1Mission . 1Diagnostic Testing and Services . 1Billing. 2Disclosure of Results . 3Location . 4Contact Information . 5Our Management Team . 6Accreditations. 7General Submission Procedures . 8Submission Forms. 8Whole Animal Submissions . 9How to Package Your Samples for Submission. 9Submission Methods . 12Common Disease Conditions . 14Abortion . 14Diarrhea . 16Pneumonia . 19Fish Pathology and Diagnostic Testing . 22Departments, Fee Schedules and Submission Procedures . 24Pathology . 24Post Mortem Examination . 25Tissue Specimens . 26Abortion Specimens . 26Specimen Packaging and Shipment . 26

Pathology (Necropsy): Tests and Fees . 28Parasitology . 30Parasitology: Tests and Fees. 30Bacteriology . 31Antimicrobial Susceptibility Testing. 32Specimens for Bacterial and Fungal Culture - Submission Requirements . 32Collection and Storage . 32Submission Guidelines . 32Bacteriology: Tests and Fees. 35Histopathology. 37Histopathology - Submission Requirements . 37Preparation of Fixed Tissue with Formalin. 38Histopathology: Tests and Fees . 38Molecular Diagnostics . 39Diagnostic and Proficiency Testing. 39Molecular Diagnostics - Submission Requirements . 39Collection and Storage . 40Molecular Diagnostics: Tests and Fees . 40Serology. 46Serology Samples - Submission Requirements. 46Guidelines for Serum Samples. 47Serology: Tests and Fees . 48Virology. 49Specimens for Virology and Molecular Diagnostics - Submission Requirements. 50Virology: Tests and Fees . 51External Testing and Fees . 54External Lab Testing - Client Requested . 54Notifiable Diseases. 55Reportable Diseases . 59Additional Information Regarding Common Notifiable and Reportable Diseases. 64

Salmonella Enteritidis Facts for Small Flock Owners . 64Historic Information on West Nile Virus Infection in British Columbia . 68Wildlife Diseases of Concern . 68Bovine Tuberculosis . 68Chronic Wasting Disease . 69White Nose Syndrome . 70Pneumonia in Wild Sheep . 71Frequently Asked Questions . 72

General InformationThe Animal Health Centre works to diagnose disease and monitor animal health which is essentialto British Columbia’s agrifood industry. Laboratory findings are used to monitor the status ofanimal health in British Columbia. Ongoing surveillance and reporting of listed diseases toappropriate agencies is a crucial role of the Animal Health Centre and has important implicationsfor product export certification. In addition, the diagnosis of diseases that may be transmitted fromanimals to people is important to health authorities.Staff veterinarians and laboratory scientists investigate and identify major livestock andcompanion animal diseases that could have potentially devastating effects on the food supply orpose a threat to public health. Up to 70% of all diseases affecting people come from animals. Aneven larger percentage of newly-emerging diseases originate with animals.Animal Health CentreThe Animal Health Centre provides world class veterinary diagnostic services that protect thehealth of all animals in B.C. to support disease prevention, control and eradication. The AnimalHealth Centre protects human health, with the timely and accurate diagnosis of zoonotic diseasesthat transmit from animals to humans, in both the public health and the food safety sectors. TheAnimal Health Centre is a leading accredited full-service veterinary laboratory in western Canada,offering more than 400 laboratory diagnostic tests for agents that may be found in wild anddomestic birds, mammals, fish, reptiles and amphibians.MandateThe mandate of the Animal Health Centre is to diagnose, monitor and assist in controlling andpreventing animal disease in British Columbia.MissionOur mission is to support the sustainability of animal agriculture while serving to protect the wellbeing of the people of British Columbia through the surveillance, regulatory compliance, riskassessment and development of strategies to address risk.Diagnostic Testing and ServicesA full range of fee-for-service diagnostic testing, including Bacteriology, Histopathology,Molecular Diagnostics, Necropsy, Serology and Virology are accepted from veterinarians,livestock producers, the general public and other government agencies.While primarily concerned with food-producing animals, the Animal Health Centre also providesdiagnostic services for companion animals, captive and free-ranging wildlife, zoo animals, fish,fur-bearers and bees.1

BillingIn accordance with the Animal Health Act, the Lieutenant Governor in Council may makeregulations designating laboratories as provincial laboratories, and respecting fees that may becharged by provincial laboratories, including fees for diagnostic examinations and post-mortemservices, services performed for the purpose of gathering evidence for a legal proceeding andproviding analytical or interpretive reports. Section 5 of the Laboratory Fees Regulation states, “a provincial laboratory may chargethe fees set out in the [Fee] Schedule in respect of services performed or laboratoryreports provided under the Animal Health Act or the Ministry of Agriculture and FoodAct”. Note that all charges are payable at the time of submission. Results will not be releaseduntil payment has been received. Accepted Methods of Payment: VISA, MasterCard, American Express, Debit, Cheque(payable to “Minister of Finance”) or Electronic Fund Transfer. Fees listed do not include applicable taxes. Out of province submissions will be charged at the fee plus 50%.2

Disclosure of Results The veterinary professional responsible for your case will report their findings, diagnosesand recommendations for control (if applicable), in a written report. Whenever possible, it is preferable for our veterinary professionals to report directly to aveterinarian. The veterinarian has an established relationship with the animal and client,and can help to interpret results for the client. If results are urgently required, contact the Animal Health Centre directly at 604-556-3003before submitting the specimen. Preliminary results can often be available within a day ortwo by contacting the Animal Health Centre directly. Results are sent primarily to the client. Reports will only be sent to third parties if indicatedon the sample submission form. Exceptions will only be made in the instance that anotifiable or reportable disease is suspected. In this case both the Chief Veterinarianof British Columbia and the Canadian Food Inspection Agency (CFIA) must benotified. Cases of suspected animal abuse will also be reported to the Society for thePrevention of Cruelty to Animals (SPCA). Results will only be sent upon receipt ofpayment.3

LocationThe Ministry of Agriculture’s Animal Health Centre is located in the Abbotsford AgriculturalCentre. Our mailing address is:1767 Angus Campbell RoadAbbotsford BC V3G 2M3From Highway 1 Take exit 92 toward MissionMerge onto BC-11 NTurn Right onto Delair RoadTurn Right onto Angus Campbell Road4

Contact InformationLocal phone: 604-556-3003Toll free: 1-800-661-9903Front office email: PAHB@gov.bc.caSubmission forms can be emailed to: PAHB.Submissions@gov.bc.caWebsite: www.gov.bc.ca/animalhealthcentreHours of operation: The Animal Health Centre is open Monday to Friday from 8:30 A.M. until4:30 P.M. Please note that the Animal Health Centre is closed on Statutory Holidays.5

Our Management TeamUrsula Viney, CPA, CGAExecutive Director Plant and Animal Health BranchPhone: 778-666-0546Email: Ursula.Viney@gov.bc.caChelsea Himsworth, DVM, MVetSc, PhD, Dipl ACVPLeader, Veterinary Science and DiagnosticsPhone: 778-666-0589Email: Chelsea.Himsworth@gov.bc.caTomy Joseph, DVM, M.Sc., PhDDirector (acting)/Veterinary VirologistPhone: 778-666-0522Email: Tomy.Joseph@gov.bc.caKazal Krishna Ghosh, DVM, MS, MSc, Dipl. ACVMVeterinary MicrobiologistPhone: 604-666-0603Email: Kazal.Ghosh@gov.bc.caErin Zabek, BSc, RMCCM.Biological Safety Officer/Section Head /Laboratory OperationsPhone: 778-666-0575Email: Erin.Zabek@gov.bc.ca6

AccreditationsThe Animal Health Centre is accredited by:The American Association of Veterinary Laboratory Diagnosticians (AALVD) - fullaccreditation: https://www.aavld.org/accredited-labsThe Standards Council of Canada (SCC) for ISO/IEC 17025:2017 - standard for accreditedtechniques and specific tests listed on our scope of -plant-andanimal-health-laboratories7

General Submission ProceduresSubmission of samples or whole animals can be facilitated in several ways. Please call the AnimalHealth Centre at 604-556-3003, or visit our website for more information. All submissions musthave a submission form accompanying them. If the correct paperwork is not received within 24hours for fresh fish, horses, and cattle, then the animal will be disposed of without necropsy ortesting. All other animals and samples will be held for 5 business days prior to disposal. Pleaseunderstand that any delay may compromise the diagnostic integrity of the specimen.Submission FormsSubmission forms may be completed at the branch either written or typed at the front office of theAnimal Health Centre reception desk. We will only accession animals and samples submitted withthe current official Animal Health Centre submission forms, which can be accessed via our websitewww.gov.bc.ca/animalhealthcentre. Non-official (e.g., clinic-specific) forms, prior versions ofAnimal Health Centre forms, and species inappropriate forms (e.g., using the Mammalian formfor an Avian submission) will not be accepted.A completed submission form must be received with every submission in order for that submissionto be processed. Incomplete submission forms will not be processed. At a minimum, this shouldinclude: Complete contact information for the client and veterinarian (if a veterinarian is involved) The species and/or breed, sex, and age of the animal A complete history of affected animal(s) indicating suspected disease(s) if possible. Takingthe time to write out a thorough but concise history will help to ensure that all factors aretaken into consideration when examining the animal/specimen, selecting tests, and makingthe final diagnosis and recommendations. Medical records or other laboratory results (i.e.,bloodwork) can be included with the submission form if they are relevant to the case.However, please do not attach medical records in lieu of writing out a complete history onthe submission form. Any relevant treatments or vaccinations.If the form we receive is incomplete or incorrect, then our front office staff will contact you torequest the needed paperwork/information. However, it is ultimately the responsibility of thesubmitter to ensure that the correct paperwork/information is submitted to the Animal HealthCentre in a timely manner.Please note that samples submitted without a submission form will not be accepted. Animals orsamples sent to the Animal Health Centre with an incomplete submission form will be placed onhold. Fresh fish, whole horses and cattle will be held for 24 hours only. All other samples will beheld for 5 business days prior to disposal.8

For necropsy cases and ‘portions’ cases (i.e., cases where a necropsy has been done in the field),tests will be selected at the discretion of the pathologist.For all other cases, clearly indicate on the submission form the specific test(s) requested.The submission form should accompany the animal or specimen. For specimens shipped to theAnimal Health Centre, enclose submission information in a separate plastic bag and pack it at thetop of the box.The Animal Health Centre is able to send animals 40 kg for private cremation at the discretionof the pathologist. For animals 40 kg, special cremation options are available. Contact the AnimalHealth Centre for more information about cremation.Whole Animal SubmissionsUp to 3 animals, 8 birds or 5 fish can be submitted within a single submission so long as theanimals within the submission represent the same disease process (e.g., show similar clinical signsof disease). If separate tests on individual animals are required, then those animals should besubmitted separately.Samples from animals included in a single submission will be pooled and a single report will beissued. If it is important to separate animals for testing, then that should either be clearly indicatedon the submission form (extra charges will apply) or those animals should be separated out intodifferent submissions.Poultry 8 kg and fish of any size may be submitted alive for euthanasia (live fish must besubmitted in water).All other animals must be submitted dead. The Animal Health Centre will not euthanize liveanimals other than poultry or fish.How to Package Your Samples for SubmissionGeneral Sample Packing Guidelines: Before collecting specimen(s), check the sample requirements for the particular test yourequire. You can find this in the appropriate section of the fee guide or online at:www.gov.bc.ca/animalhealthcentre. Ensure that all samples are adequately labelled with the animal identification and any otherrelevant information. Paperwork should be placed in a zip lock bag within the outer layer of packaging to prevent itfrom getting wet from ice packs or contaminated from the sample itself.9

Ideally do not tape paperwork to the outside of the shipping box as if it is not seen right awayit is often cut when opening packages upon arrival. When sending in samples from multiple animals, be sure to indicate whether you would likethe samples to be pooled or run individually so we know how to process the samples. Eachsample container should be labelled clearly with the animal identification, so it is known whichsample is from which animal.Liquid Samples or Samples in Formalin: Ensure sample is in a twist top, leak proof container. Tape around the top of the lid to prevent leakage and the lid from coming off. Ensure that this container is placed inside another sealed container, i.e. an individual zip lockbag, or other larger container. It is best to send in a box with packing material around it to reduce impact during shipping. If sending in a shipping bag, ensure that the sample is tightly wrapped with bubble wrap orother protective material. Paperwork should be placed outside of the original packaging for the formalized/liquidsamples in its own zip lock bag. This way if the sample leaks or the ice packs sweat, thepaperwork does not get wet or contaminated.Examples of Well Packaged SamplesIndividually labelled, sealedcontainers for fecal analysis.Twist top container, tapedaround the edges to preventleakage.10

Place inside of a ziplock bag toprevent leaking from the firstcontainer.Place inside a third protectiveouter layer. Ice packs added tokeep sample frozen/cool duringtransport. Add packing materialif there is a lot of space in the boxto prevent the sample frommoving around too much.Place paperwork in its own ziplockbag to keep it dry and place it on theouter layer of the package, but stillwithin the box.11

Improper Sample Packaging: Contaminates paper work.Can make submission form unreadable causing delays in the processing time of your sample.Paper soaked in formalin from animproperly packed sample.Submission MethodsEnsure that containers used to transport and ship specimens meet the requirements of theTransportation of Dangerous Goods Act (TDG). In most cases, Type 1B packaging, i.e., watertightinner packaging surrounded by absorbent material, watertight secondary inner packaging andsturdy outer packaging (corrugated cardboard) should be used. For cases where there may be azoonotic pathogen present, ensure that samples are transported and labelled accordingly.For more information about TDG visit their website at: www.tc.gc.ca/eng/tdg/act-menu-130.htmDirect Delivery Weekday delivery between 8:30 a.m. and 4:30 p.m., deliver as early in the day as possible. Large animals are not accepted for unloading between 12:30 p.m. and 1:00 p.m. Please check in with the front office and complete the appropriate submission form beforeunloading whole animals. Live animal submissions must arrive in a disposable container. Once a container enters thepost mortem room it will be destroyed.Mail12

Samples must be well preserved so that they arrive in suitable condition for examination. Samples must be adequately packed so they do not leak per TDG guidelines. Each tissue must be submitted in a separate, labelled, sealed bag. Please indicate:othe source of the sample (e.g. rectal, liver, etc.), andothe number of vials included with each submission. Separate labelled samples are required for each test (e.g. bacteriology, virology, etc.) asthey are sent to different sections of the Animal Health Centre for processing. Additionalcharges will apply for any sample that needs to be split for processing. Paired sera must be tested on the same day to render a meaningful comparison. A completed history sheet and submission form is best received in its own separate plasticbag. Clearly indicate the total number of items submitted for each submission, on the submissionform.13

Common Disease ConditionsIf in doubt about how to proceed with a case submission, please consult our lists of Tests andPreferred Specimens on our website: www.gov.bc.ca/animalhealthcentre or consult the dutypathologist at the Animal Health Centre at 1-800-661-9903 or 604-556-3003 during regular officehours.AbortionA. History:Please provide as complete information as possible on the dam, herd, or management system: e.g.herd size, general level of management and herd health status, recent additions to herd, numbersof pregnant animals in herd, previous abortions or indications of infertility, number ofabortions/stillbirths/weak neonates in herd, parity of the dam, stage of gestation, nutritional statusof dam, previous illnesses or stresses, vaccination status of the dam and any signs of illness. TheAHC Mammalian Submission Form provides a valuable template to compile pertinent farmidentification, historical information and signalment.B. Selecting and Submitting Samples: Based on the degree of autolysis, maceration and possible scavenging, the placenta andfetal remains should be submitted for post mortem examination. Submission of a fetus,placenta and if possible, a serum sample from the dam afford the best likelihood ofrendering a diagnosis. As an agent and/or lesions may not be present in all fetuses, if possible, please submitmultiple fetuses (up to 3 fetuses may be presented in a single submission). If submitting the entire fetus is not possible, please submit as complete a set as possible oftissues as listed below. A serum or milk sample from the dam may also be provided.C. Samples Required: Frozen or refrigerated: for routine bacteriology, virology, molecular studies, trace mineralanalysis and ancillary diagnostic studies, fresh tissues, including: placenta, stomachcontents (1-2 ml), fetal heart blood (1-2 ml), heart, lung, spleen, liver, kidney, and brainshould be collected. Antibiotic resistance profiles may also be requested (Kirby Bauer discdiffusion). Formalin fixed: placenta, lung, liver, kidney, spleen, heart, adrenal gland, thymus, thyroid,small intestine (2 pieces), large intestine with meconium (2 pieces including meconium),brain, eyelid, and skeletal muscle.14

Bovine Abortion Bacteriology: placenta, lung, and stomach content (1-2 ml). Histology: placenta (cotyledon and intercotelydonary regions), lung, liver, kidney, spleen,heart, adrenal gland, thymus, thyroid, small intestine (2 pieces), large intestine withmeconium (2 pieces including cecum and spiral colon), brain, eyelid, and skeletal muscle.o In-house immunohistochemistry is available on formalin-fixed tissues for BovineViral Diarrhea Virus (BVDV), Bovine Herpesvirus-1/ Infectious BovineRhinotracheitis (BHV-1/IBR), Leptospira spp., and Neospora caninum. PCR/Virology: heart, lung, spleen, liver, kidney, and brain. Radioimmunodiffusion/Ig Quantification/Serology: heart blood (1-2 ml). Serology/Trace Mineral/Vitamin: dam serum submission.Ovine/Caprine AbortionNote: all small ruminant abortions should be considered a potential zoonotic risk due toCoxiella burnetii infection and should be handled with appropriate precautions, includinguse of personal protective gear and thorough hand washing and disinfection of fomites. Bacteriology: placenta, lung, and stomach content (1-2 ml). Histology: placenta (cotyledon and intercotelydonary regions), lung, liver, kidney, spleen,heart, adrenal gland, ileum, thymus, thyroid, small intestine (2 pieces), large intestine withmeconium (2 pieces including cecum and spiral colon), brain, eyelid, and skeletal muscle.o In-house immunohistochemistry is available on formalin-fixed tissues forToxoplasma gondii and Neospora caninum and tissue blocks can be sent to areference laboratory for Coxiella burnetii PCR/Virology: heart blood (1-2 ml), heart, lung, spleen, liver, kidney, and brain. Serology/Trace Mineral/Vitamin: dam serum submission.Equine Abortion Bacteriology: placenta, lung, stomach content (1-2 ml). Histology: placenta (including samples of cervical star), lung, liver, kidney, spleen, heart,adrenal gland, ileum, thymus, thyroid, small intestine (2 pieces), large intestine withmeconium (2 pieces including cecum and colon), brain, eyelid, and skeletal muscle.o In-house immunohistochemistry for Equine Herpes Virus 1 (EHV-1) is availableon formalin-fixed tissues. PCR/Virology: heart blood (1-2 ml), heart, lung, spleen, liver, kidney, and brain.15

Serology/Trace Mineral/Vitamin: dam serum submission.Porcine Abortion Bacteriology: placenta, lung, stomach content (1-2 ml). Histology: placenta (including samples of cervical star), lung, liver, kidney, spleen, heart,adrenal gland, ileum, thymus, thyroid, small intestine (2 pieces), large intestine withmeconium (2 pieces including cecum and colon), brain, eyelid, and skeletal muscle.o In-house immunohistochemistry is available on formalin-fixed tissues for PorcineReproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Circovirus 2(PCV-2). PCR/Virology: heart blood (1-2 ml), heart, lung, spleen, liver, kidney, and brain. Serology/Trace M

The Animal Health Centre works to diagnose disease and monitor animal health which is essential to British Columbia's agrifood industry. Laboratory findings are used to monitor the status of animal health in British Columbia. Ongoing surveillance and reporting of listed diseases to appropriate agencies is a crucial role of the Animal Health .

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