Flash Purification, Metal Scavenging, Reagents & Scavengers

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Biotage Scale Up SolutionsFlash Purification, Metal Scavenging,Reagents & Scavengers

CONTENTS124This is BiotageThe World of BiotageRegulatory and QualityFlash Purification810131618202224262832Flash PurificationBiotage Flash Purification PlatformsFlash Purification Case HighlightsBiotage SelektIsolera LSBiotage Flash 75Biotage Flash 150Biotage Flash 400UV MonitorPurification ColumnsScaling Columns Metal Scavenging36414244Metal ScavengingMetal Scavenging Case HighlightsMetal Scavenging Tool KitsBiotage Metal ScavengersReagents and Scavengers4851Efficient Delivery of Reagent to ReactionsReagents and Scavengers Scale-up and Method Development Resources586466Scale-up and Method Development ResourcesService SolutionsOrdering InformationHighlights12Purification of Lipids usedin mRNA Vaccines14PhospholipidPurifying 147 kg onIonizable lipidNatural ProductNucleic acidCholesterolPegylated lipid402MP-TMT Best in Class13Biotage Flash 400 in APIManufacturing Workflow15Type 2 Diabetes Candidate41Metal Scavenging vs.Recrystallization Biotage Scale Up Solutions Biotage 2022

This is BiotageThis is BiotageFlash Purification SolutionsBiotage is a Global Impact Tech Company committed to solvingsociety’s problems. We offer workflow solutions and productsto customers in drug discovery and development, analyticaltesting and water and environmental testing.Biotage is contributing to sustainable science with thegoal to make the world healthier, greener and cleaner –HumanKind Unlimited. Our customers span a broad range ofmarket segments including pharmaceutical, biotech, contractresearch and contract manufacturers as well as clinical, forensicand academic laboratories in addition to organizations focusedon food safety, clean water and environmental sustainability.Biotage is headquartered in Uppsala in Sweden and employsapproximately 485 people worldwide. The Group had sales of1,092 MSEK in 2020 and our products are sold in more than70 countries. Biotage’s share (BIOT) is listed in the Mid Capsegment on the NASDAQ Stockholm.Metal scavengersReagents and ScavengersHQ – Uppsala, SwedenCardiff, UKSalem, USASan Jose, USACharlotte, USALund, SwedenOxford, UKSouthampton, UKSeoul, South KoreaNew Delhi, IndiaTokyo, JapanOsaka, JapanShanghai, ChinaScale-up ResourcesOffice SiteR&D and ManufacturingDirect SalesDistributor1

The World of BiotageThe World of BiotageBiotage offers workflow solutions and products tocustomers in drug discovery and development, analyticaltesting and water and environmental testing.BiomoleculeproductiontioPlasmid CloningdGranngginiokuptsh caFla urifiPWorenavScSynthesisnid iom atas cPl urifiPBiotageSCALE-UP LINEPurificationCentrnBIOMOLECULE LINEecsfanTrPlasmidConstruct DesignEvaDrug DesignSample PreparationePrtenmattreORGANIC LINEZone?Sample CollectionANALYTICAL LINE2BIOTAGE SUBWAYBiotage Scale Up Solutions Biotage 2022

The World of BiotageFlash Purification SolutionsSDS-PAGEralBiomoleculePurificationScaling UpEvaporationReagents and ScavengersWhen a drug candidate is going to betested, volumes must be scaled up.This requires larger capacity equipmentfor purification. From traditional smallmolecules to peptides, natural productextraction, targeted synthesis of targetsin the latest high-tech HP-APIs, or novellipids used in COVID-19 formulations,Biotage purification platforms andscavenging technologies are trustedand underpin efficient processes,from research to cGMP campaign orcommercial production, the world over.Metal scavengersELISAScale-up ResourceseLiGaqusCMidashrHCsShr igomhopem Patctat reogrosorasgmra urephetpyryhyaporation3

Regulatory and QualityAbout IconsRegulatory and QualityIt is critical that equipment used in process is reliable, reproducible,robust and traceable. Biotage has worked with suppliers and a numberof internationally recognized bodies to attain global standards ofbenchmarked quality and compliance in our scale-up products.SustainabilityAt Biotage Sustainability is in the forefront of our work. Ourtagline “HumanKind Unlimited” underscores our ambition tostrive towards improving global health. Whilst we focus on thedevelopment of our products, we also monitor our own wastematerials and emissions and how they affect our environment.From a social perspective, we focus on our employee’s jobsatisfaction, their working environment and ensuring we offerrewarding terms and conditions.On an international perspective, we support the Universal Declaration of Human Rights andthrough our Code of Conduct ensure our interactions withstakeholders adhere to its principles. We fully support effortsagainst corruption and enhancing local societies through taxcontributions, diversity, work opportunities, fair competition,consumer protection and product safety. Globally, we are asignatory of the United Nations Global Compact, which encourages businesses to embrace sustainability and socialresponsibility.SDS supportBiotage supported reagents and scavengers are made in anISO9001:2008 compliant manufacturing facility. Each materialis batch and lot controlled, with benefits of full traceability.The products are readily available in multi-kg quantitiesoff-the-shelf and supported by a comprehensive regulatoryqualification support package comprising certificate ofanalysis, extractables, chemical loading, lot information, batchidentity and consistency, MSDS/SDS, and full instructions andsuggestions for use. Biotage can provide extensive technicalsupport relating to the efficient use of these products in variousprocesses.4Environmental AccreditationsNatural Resource Wales, Environmental PermitRegulations 2010, registration #EPR/DP3832EFPreserving our shared environment is fundamental to Biotage,as it is to our employees, customers, and other stakeholders.Biotage has attained an environmental permit from NaturalResources Wales, under The Environmental Permitting (England& Wales) Regulations 2010. Our registration number is EPR/DP3832EF.Quality AssuranceISO9001QualityManagementISO 9001:2008Biotage is accredited to the world-renownedBritish Standards Institute ISO 9001:2008standard (registration number FM31206), with the scopedefined as “Design and manufacture of sorbent and resinmaterials for sample preparation and purification products.Manufacture of laboratory automation equipment.” Quality isbuilt into our everyday principles and practices. 2015 was amilestone in our commitment to quality as we have now heldthis ISO accreditation for over 20 years. All Biotage productsare manufactured in controlled conditions. Consumables are lotcontrolled and traceable, and instruments carry ASME, TÜV andCE registration as necessary.ISO14001:2004Biotage is also accredited to the British Standards InstituteISO 14001:2004 standard (registration number EMS640981)with the scope defined as “Design and manufacture of sorbentand resin materials for sample preparation and purificationproducts. Manufacture of laboratory automation equipment.”Ensuring we play our part responsibly is important to us andour registration to ISO 14001:2004 system was yet anothermilestone in our achievements and commitment to all ourfutures.Biotage Scale Up Solutions Biotage 2022

Regulatory and QualityFlash Purification SolutionsImpurity and Chemical ControlTSE/BSE StatementsAll Biotage polystyrene backbone resin materials and silicabased products in the consumables range are produced fromeither petroleum based chemicals or inorganicsalts. Raw materials are traceable and do not contain materialsof animal or biologicalorigin; nor do ancillary chemicals used in production processes.ICH Q3DThe ICH (International Conference on Harmonisation) Q3Dguidelines were accepted in December 2014. The guidelinesrelate to impurities in new drugs and formulations, and affecta large number of pharmaceutical industries. To support ourclients commitment to ICH Q3D, Biotage has liaised withsuppliers to provide data to support a statement which can berisk assessed in the context of the proposed industrial process,supporting compliance. Reagents and ScavengersBiotage scale up purification platforms come complete with aCE mark, an ASME “UM” stamp and are certified for usage inJapan, Europe and North America. To assist you in developingSOPs, Biotage Flash 75/150/400 systems are fully supportedby user friendly and icon-based instructions manuals.Biotage Flash 75/150/400 are supported by a comprehensiveengineering documentation package, a certificate of performance and a certificate of compliance for validation filing.Please enquire for more details.We all try to avoid plastics, but where a process or industryapplication demands a single use plastic for the purpose ofimpurity control, or safety/risk mitigation, we can provideseveral assurances. Biotage Flash 75, Flash 150 and Flash 400cartridges are constructed of medium or high density polyethylene or polypropylene, and meet the FDA extractable requirement specified in 21 CFR 177.1520. Resin and silica productsare efficiently washed and tested during production phases tominimize any downstream contamination risk and reduce theburden of solvent utilization for our client base. Extractabledata for all scavengers and stationary phases is available onrequest.Our metal scavengers have been demonstrated to bethe cleanest available. In a study a few years ago, we simplyextracted small quantities of metal scavenger from varioussuppliers, into a number of different commonly used solvents.We analysed the extracts by GC. Biotage Si-Thiol (a metalscavenger) significantly cleaner than other thiol based metalscavengers, resulting in much cleaner extracts and the assurance that impurities would not inadvertently be added to API orintermediates during metal scavenging processing steps.Metal scavengerscGMP qualifiedExtractablesShelf-Life StatementsDue to the highly stable nature of components and rawmaterials, the shelf-life of Biotage consumables products isactually very long-indefinite, however for Quality Assurance orplanning purposes; our clients typically use 1 year as guidancefor expiration. See individual product notes for further details.Scale-up ResourcesREACHATEXATEX is the framework of legislation and guidance for controlling explosive atmospheres and the standards of equipmentand protective systems used in them within Europe. BiotageFlash 75/150/400 systems are compliant with the ATEX ProductDirective, 2014/34/EU.Our suppliers have confirmed that the raw materials used tomanufacture our products do not contain any substances thatrequire registration. We will continue to monitor our suppliersto ensure that any changes in raw materials will be recordedand if required they will be registered as stated in the REACHDirective. Products manufactured and sold by Biotage do notcontain any of the listed Substances of Very High Concern inamounts greater than 0.1% as defined in REACH article 57,Annex XIV.5

Regulatory and Quality6Biotage Scale Up Solutions Biotage 2022

Regulatory and QualityFlash Purification SolutionsMetal scavengersReagents and ScavengersFlash PurificationScale-up Resources7

Flash PurificationFlash PurificationIn 1994 Biotage was the first company to develop pre-packed cartridges for flashpurification and has been the leader in quality, performance, and innovationever since. Biotage has a long history with purification instruments, pioneeringinnovation since the late 1970s. Our research scale purification instruments arethe most technologically advanced and effective purification systems available.Our method development and purification algorithms helpscientists convert traditional regular flash purification to faster,greener, and more economical processes for reliably isolatingpure compounds at scale. Our development systems enableyou to leverage these value added features in silica choice andflow rate in method development, and apply key benefits tolarger scale purification, radically enhancing the efficiency of aproduction processes. Many of the high performance sphericalstationary phases (which can accept twice a normal sampleload, or give higher plates when packed into columns) areavailable as standard in our large scale development or processcartridge range.Purification Platforms and accessoriesBiotage lab scale systems come with software that can automatically create linear gradients from TLC Rf spots; and thenautomatically convert those linear gradients into step gradientsfor application on a larger scale. Each large scale system maybe configured with either ‘M’ or ‘L’ cartridge capability to furtherFlash Purification Systemsand Platformsincrease its flexibility and range.Moving from a traditional lab based synthetic process to morecommercially orientated and efficient processes with a limitedtimeline used to be quite problematic, however advances inscale up purification techniques along with improvementsin stationary phase science has meant that many of theconveniences previously enjoyed by the lab scale medicinalchemist are now available for scale up, along with the additionalbenefits conferred by process efficiencies. Biotage Flash 75,150 and 400 systems are extremely tough, economically viableindustrial purification systems that have been developed forthis purpose. Scaling up flash purification methods is easy andstraightforward. Any method developed using a Biotage labscale cartridge can be transferred to a larger cartridge usingthe scale-up factors in the table below. Once the development cartridge is determined, the lab scale method can bereplayed, providing peak elution profiles for the scale up systemconsistent with the development process and suitable for finaloptimization. Stationary PhasesScale up resources and calculationssee page 28see page 57see page 168Biotage Scale Up Solutions Biotage 2022

Flash PurificationFlash Purification SolutionsFlash chromatography is a preferred purification technique of organic,medicinal, natural product chemists, and more recently peptidechemists because it has the power to separate a broad variety ofcompounds more efficiently than other crude purification techniquessuch as crashing out of solution or liquid-liquid extraction.Scope and Application GuideNormal PhaseReversed PhaseAmino PhaseAlkaloidsCocaine, morphine, nicotine, quinine Amino acidsAnalgesicsAspirin, acetaminophen, ibuprofenAromatics Basic PhospholipidsNatural productsTerpenes, saponins, polyphenols (Oligo) nucleotidesSteroidsTanninsVitaminsTocopherols (vitamin E), retinol(vitamin A), vitamin D, vitamin K Scale-up Resources Peptides Reagents and ScavengersExamplesMetal scavengersProduct 9

Biotage Flash Purification PlatformsBiotage Flash Purification PlatformsMeet the FamilyFlash SystemIsolera Biotage SelektIsolera LS FormatScaleDevelopmentDevelopmentDevelopment & productionInput Sample SizeNormal Phase (g)80150*150*Flow Rate (mL/min.)200300500Solvent Reservoir Volume (L)Multiple bottles/drum sizes possibleMultiple bottles/drum sizes possibleMultiple bottles/drum sizes possibleCartridge Size d x h (mm)VariousVariousVariousCompatible cartridgemass***5–900 g5–1800 g50–1800 gSystem Part NumberSeveral modelsavailableSeveral modelsavailableSeveral models availableAdditional CompressionModules*** With pressure releasing safety valve kit P/N 417115SP for cartridges with CV 0.8 L (i.e. 340 g).** Additionally available and interchangeable within the M/L format to extend the range of the systems. See ordering information section for more detail.*** See detailed column silica masses in the resrouces and scale up guidance in this brochure.10Biotage Scale Up Solutions Biotage 2022

Biotage Flash Purification PlatformsFlash Purification SolutionsMetal scavengersBiotage Flash 75Biotage Flash 150 MBiotage Flash 400 0010006000600012123760N/AN/A75 x 15075 x 300150 x 300150 x 600400 x 300400 x 600400 g800 g2.5 kg5 kg20 kg40 eavailableavailableavailableScale-up Resources50Reagents and ScavengersDevelopment & production 11

Flash Purification Case HighlightsFlash Purification Case HighlightsPhospholipidIonizable lipidNucleic acidCholesterolPegylated lipidCommon examples of lipid purification using normal phase and reversed phase flash.Scaling Up with Flash 400 for the Purificationof Lipids used in mRNA VaccinesCase StudyIn 2020, Biotage collaborated with the specialty chemical company Croda on aproject focused on the production of a potential key novel small-molecules usedto stabilize mRNA in COVID-19 vaccine formulations, for global distribution.The Biotage team contributed their expertise in large scale andcommercial purification and with the help of Biotage Flash 400,Croda managed to scale up their processes from developmentto production in just four months. This not only resulted in astable, commercial-grade manufacturing process, it also savedCroda valuable time and reduced their solvent consumptioncompared to existing, traditional methods.”This is a success story that we are proud to share. Usually ittakes 2–3 years to complete major projects such as this but in 6months we got the technical solution in place. Using the BiotageScale-Up platform saved enormous amounts of solvents. Crodadesigned and built a new factory in six months for this purpose.We are proud of the hard work our respective organizationshave achieved and we feel proud of being part of solving aserious societal problem using Smart science to improve lives”– Steve Mellor, Research & Technology Director, CrodaRead morePPS654: Collaborating with Croda to helpsupply lipids for the global response toCOVID-19 and mRNA vaccine development.Smart Science to Improve Lives Croda is the name behind thehigh performance ingredients andtechnologies in some of the biggest, mostsuccessful brands in the world: creating,making and selling speciality chemicalsthat are relied on by industries and consumers everywhere.https://www.croda.com/en-gb12Biotage Scale Up Solutions Biotage 2022

Flash Purification Case HighlightsFlash Purification SolutionsMetal scavengersBiotage Flash 400 system in use at CordenPharma. Adopting Biotage Flash 400 Into anExisting API Manufacturing Workflow Biotage Flash 400 large-scale chromatographysystem and pre-packed cartridges were evaluatedalongside a custom-made alternative as part of aplan to increase the efficiency of the workflow. Thepurification was of a large multi-kg batch of crudeAPI (the nature of the API was proprietary).In total, 550 kg of loose silica was replaced by one Flash 400column (40 kg) and 5.5 m less waste was produced by the morestreamlined process, which was easier to integrate into theproduction environment, resulting in more efficient planning forresource needs in future projects.Reagents and ScavengersCase Study3Read morePPS647: Evaluating the adoption of theBiotage Flash 400 system into an existing APImanufacturing workflow at CordenPharmain Switzerland CordenPharmaScale-up ResourcesThe original purification process custom-built for API manufacture involved using a filter as a housing for silica, and anexternal pump to move the solvent. New process using BiotageFlash 400 reduced the overall process time from 18 days (for2 operators) to 6 days, retaining the same excellent purity of89.8% by HPLC-UV, but with an increased mass recovery of34.4 kg (89.8%). The overall operating costs of the process werereduced by 50%. There were other noted process advantages.Due to the higher column loading, the concentration of elutedproduct was much higher using Biotage Flash 400 silica, saving5,400 L of solvent and therefore much less evaporation wasrequired.CordenPharma is a full-serviceCDMO for a global market,specializing in APIs, drug products,and associated packaging servicesoperating through a growing network of cGMP facilitiesacross Europe and the US organized under five technologyplatforms: Peptides, Lipids & Carbohydrates; Highly Potent& Oncology; Injectables; Small Molecules; Antibiotics.13

Flash Purification Case HighlightsFlash Purification Case HighlightsPurifying 147 kg of Natural ProductSatori PharmaceuticalsBiotage Flash 400 cartridges were used to purify large crude batches for anAlzheimer’s drug candidate. Gamma-secretase modulators (GSMs) are promisingcompounds for Alzheimer’s disease therapy. As part of thesynthetic pathway to obtain the GSM SPI-1865, an internationalresearch group synthesized large quantities of two cycloartenoltri terpenoid glycosides from roots of Actaea racemosa, knownas black cohosh. In doing so, no less than 147 kg of extractsolution was purified on a Biotage Flash 400L KP-Sil cartridge,installed on a Biotage Flash 400 system. The fractionscontained 11.44 kg of extracted product. Ruichao Shen, a leading scientist at Satori PharmaceuticalsInc. explains: “The problem we faced was to find an efficientmethod to purify the crude mixture as fast as possible andmaintain a good recovery at the same time. At that time Biotagehad the largest pre-packed SiO2 cartridge available in theindustry. We chose the product and it helped us solve theproblem well.”Read MoreRuichao Shen et al., 2014. Multikilogram-Scale Production ofCycloartenol Triterpenoid Glycosides as Synthetic Intermediatesfor a γ-Secretase Modulator. Organic Process Research &Development 2014 18 (6), 676-682DOI: 10.1021/op5000732Root nodules of Actaea racemosa, a well known medicinal herb containinga candidate precursor for a novel Alzheimer remedy.17 kg Sampleover 2 DaysA major UK Major Pharmaceutical Company usedBiotage Flash 400 for bulk drug purification.A sample (17 kg made up in DCM), containing 4 kg of productwas split and 3 x 5.7 kg injections performed. Using normalphase conditions (hexane/ethyl acetate) with a flow rate of5 L/min, 3 kg of purified product was isolated in 95% purity,representing 75% recovery. Each injection was 50 minutes andthe total time for batch purification was only 2 days.14Biotage Scale Up Solutions Biotage 2022

Flash Purification Case HighlightsType 2 Diabetes CandidateFlash Purification SolutionsUS Major Pharmaceutical CompanyA US Major Pharmaceutical Company developinga Type 2 Diabetes candidate (a G Protein-coupledreceptor 119 (GPR119) agonist) had 13 kg of racemicacetate, which was hydrolyzed in 2 batches to targetone chiral form.2 Flash 400 runs, with 6.5 kg injections was performed, usingnormal phase (KP-Sil 40–63, average 50 micron particle size)conditions and a 50/50 ethyl acetate/heptane isocratic solventmix. Final mass yield was 4.1 kg (36.5%, theoretical 5.627 kg),and due to the chemical control in hydrolysis, an ee of 99.4%Metal scavengers» Overall mass yield 4.1 kg (36.5%, theoretical 5.627 kg)» ee 99.4% (see next slide)Read moreOrganic Process Research and Development 2015, 19, 819–830 US Major Pharmaceutical CompanyReagents and ScavengersProcess Improvement with Biotage Flash 400Biotage Flash 400M system was used to improvea pre-existing but inefficient process which used astainless-steel self-packed column, and 26 x 1.5 kginjections in order to process a 40 kg batch.Scale-up ResourcesThe original SS column needed to be packed and unpackedbetween each run, and the overall cycle time of 3 days, withno silica re-use resulted in a final process that took 6 weeks tocomplete, using a 3-per-day shift pattern supporting 20 hourruns. Application of Biotage Flash 400M enabled 6 runs to becompleted per day, during a single 8 hour working shift, andthe surrounding optimization requiring only 1 week to completethe project. Only one cartridge was used, resulting in silica andlabour costs being reduced by 75%.15

ISO9001QualityManagementratorybocticPra eBiotage Selekt Good LaBiotage SelektDevelopment Scale Flash SystemBiotage Selekt Systems are the latest in state of the art automatedpurification systems designed to autonomously separate upto 150 gof sample per run, at 300 mL/min, whether natural product extracts orother organic compound mixtures from chemistry. Biotage’s approach to laboratory scale applications usingSelekt is built around speed, productivity and reduced environmental impact. This is achieved by the combination of Selektwith Sfär high-performance flash columns. And of course, themore efficient a small scale or developmental run is, the greaterthe likelihood of a robust and successful tech transfer and scaleup process.The Smallest ColumnsSelekt makes use of the Sfär flash columns. These columnshave very high loading capacity when compared to ‘traditional’flash columns, achieved through the use of high-qualityspherical silica. The result is that you can load the same amount16of sample as you can on a traditional column onto a Sfärcolumn half the size. A smaller column means reduced solventconsumption and more concentrated fractions with no loss inresolution, in a fraction of the time. Faster and greener!Greatest ProductivityThe Selekt system operates at the highest flow rates andpressures of any flash instrument. As a result Sfär columnscan be run at very high flows without compromising separations – faster flows mean faster results. Also Sfär columns areequilibrated using a patented high pressure method, resultingis ultra fast equilibration and full wetting of the column forrapid, reproducible chromatography.Biotage Scale Up Solutions Biotage 2022

Biotage SelektWEIGHT23–25 kg (50–54 lbs.)depending on the systemconfigurationDIMENSIONSCOLUMN CHANNELSTwo144 fractions with no rackchange (288 with two collection trays) using 13 x 100 mmracks.AMBIENT TEMPERATUREOperating: 15–32 C (59–90 F). Storage and transportation: -25–60 C (-13–140 F).FLOW RATE RANGE1–300 mL/min, in 1 mL/minincrements.SOLVENT SUPPLYA maximum of 4 x 5 literreservoirs on the optionalsecondary solventcontainment.INTERNAL DETECTORWAVELENGTH200–400 nm (UV) or 198–810nm (UV-VIS).RACK TYPES13 x 100 mm, 16 x 100 mm, 16x 150 mm, 18 x 150 mm, 25 x150 mm, 120 mL, 240 mL, and480 mL.PRESSURE RANGE0–30 bar (0–3000 kPa; 0–435psi).ELECTRICAL SUPPLY100–127, 220–240 VAC,50/60 Hz. Connect only to agrounded outlet.Metal scavengersFootprint: (W x D) 335/550 mmx 393 mm (13.2/21.7” x 15.5”).The width depends on usingone or two collection trays.Height: 545 mm (21.5”)excluding secondary solventcontainment.MAX NUMBER OFFRACTIONSFlash Purification SolutionsSpecificationsReagents and ScavengersLarge scale applicationsSafety firstWhen running large scale columns, Selekt can be fitted with asafety valve that prevents over-pressuring of the columns. AtBiotage safety is paramount, giving you peace of mind across allof your applications is important to us.What’s in the Box?Everything you need to get started, a base system (includingpump, specified uv detector, fraction collection tray),accessory kit including manual, documentation package,starter columns, 5–350 g column holders, 3 racks (16 x150 mm) for fraction collection, tubing, connections.Scale-up ResourcesBiotage Selekt is also capable of performing large scalepurification running 750 g and 1.5 kg columns. Scale up to largesample sizes couldn’t be easier than with Selekt. A simplescale-up route from laboratory columns means that methodscan be created using small-scales, and then quickly and easilyadapted for larger sample sizes, all in the Selekt software. Aseamless and simple approach to large sample sizes.17

Isolera LSGood LaISO9001QualityManagementratorybocticPra eIsolera LS Flash System for Scaling UpIsolera Spektra LS flash systems are automated purification systemsdesigned up to 150 g of sample per run, whether natural product extracts orother organic compound mixtures from chemistry. Isolera LS is the definitive flash chromatography system forreliable scaling up from grams to multi, even hundred gramscale purification, using the industry standard original Isolerainterface. Enjoy all of the sophisticated software featuresof laboratory scale systems in a robust instrument built forscale-up labs. Isolera Spektra LS adds an integrated pumpassisted loading mechanism for larger samples, and a funnelrack collection option which allows samples to be collected inlarger fraction collection vessels.Up to 30% solvent savings withGradient Optimization “GO”Step-gradients can be short and powerful providing separationsfor one or more compounds but can be challenging to developand optimize. Isolera Spektra provides gradient optimizationthrough TLC-to-Step Gradient technology. Using solvent and18TLC Rf data, Isolera Spektra builds a gradient to separate allthe compounds (up to 6) in the sample. This new technologywill also provide cartridge selection guidance based on thecartridge loading capacity and purification speed. The stepgradient can also be used to isolate a targeted compoundreducing run time and solvent use further.Improve fraction and compound purity withλ-All detection and PDA spectral analysisPDA scanning and λ-All technology detects any UV absorbingcompound eluting from a flash cartridge while measuring anddisplaying each eluting compound’s individual UV spectrum.Combined with novel baseline rise correction, yield losses toimproper wavelength selection and large fraction volumes areno longer a concern. The PDA spectra can be reviewed and usedto determine fraction purity eliminating the need for post-flashpurity analysis.Biotage Scale Up Solutions Biotage 2022

Isolera LSFlash Purification SolutionsBaseline CorrectionMany chromatographic solvents absorb UV light. We don’t wantto be limited in our choice of solvents, Isolera Spektra usesadvanced real gradient blanking for true baseline correction toeliminate background shifts.Digging Deeper With PDA Spectral AnalysisElute complex samples with the Quatro-binary gradientEnhance productivity with on-the-fly editingIsolera methods can easily be edited either in front of theIsolera or from the comfort of your office. Edit the gradie

innovation since the late 1970s. Our research scale purification instruments are the most technologically advanced and effective purification systems available. Our method development and purification algorithms help scientists convert traditional regular flash purification to faster, greener, and more economical processes for reliably isolating

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