UTRGV Laboratory Safety Manual

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UTRGV LaboratorySafety ManualLAST REVISED: OCTOBER 2019The University of Texas Rio Grande ValleyDEPARTMENT OF ENVIRONMENTAL HEALTH, SAFETY AND RISK MANAGEMENT 956-665-3690

Table of ContentsA. INTRODUCTION . 8Note to Users of This Laboratory Manual. 8B. RESPONSIBILITIES . 9I.University-Wide Safety Responsibilities . 9The President . 9Environmental Health, Safety and Risk Management . 9Campus Facilities Operations and Utilities . 9II.Departmental Safety Responsibilities . 10Dean . 10Department Chair and Directors . 10Chemical Hygiene Officer . 10Principal Investigator and Laboratory Supervisor. 11Employees and Other Laboratory Personnel . 11C. EMERGENCY PROCEDURES . 13I.Immediate Threat to Life or Health . 13II.Fire . 13III.Accidental Exposure or Injury . 13IV.Major Spill . 13V.Minor Spill . 14VI.Radioactive Hazard. 14VII.Biological Hazard . 14VIII.Building Evacuation . 15D. FUNDAMENTALS OF LABORATORY SAFETY . 16I.Procurement, Distribution, and Storage . 16Procurement . 16Stock Rooms/Storerooms . 16Distribution . 16Laboratory Chemical Storage . 16II.Storage Cabinets . 17Flammable Storage Cabinets . 17Acid Storage Cabinets . 18UTRGV Laboratory Safety Manual2

Compressed Gas Cylinder Cabinets. 18III.Personal Protective Equipment and Clothing . 18IV.Signs and Labels . 18V.Records . 19VI.Medical Program . 19Procedures for Authorizing Medical Treatment . 19Physician’s Written Opinion . 20First Aid . 20VII.Information and Training Program . 20Hazard Communication Training . 21VIII.Chemical Waste Disposal Program . 22Chemical Waste Containers . 22All Waste Containers . 22Accumulation of Chemical Waste . 23Labeling Chemical Waste Containers for Pick-Up . 23Submitting Requests for Disposal of Chemical Waste . 23IX.Inspections. 23X.Consequences of Non-Compliance . 24References . 24E. BASIC RULES AND PROCEDURES FOR WORKING WITH CHEMICALS . 25I.General Rules . 25Laboratory Protocol . 25Personal Safety Practices . 25House-Keeping. 25Accidents and Spills . 25Spill Response Equipment . 26Avoidance of “Routine” Exposure . 26Choice of Chemicals . 26Eating, Drinking, Smoking, etc. . 26Equipment and Glassware. 26Glassware. . 26Assembly of Laboratory Apparatus. . 27Centrifuges. . 27Ultraviolet Lamps. . 27Lasers. . 28Separatory Funnels. . 28UTRGV Laboratory Safety Manual3

Cooling Baths and Cold Traps. . 28Vacuum Pumps. . 28Electrical. 28II.Personal Protection. 29Protective Eyewear. . 29Protective Gloves. . 29Other Protective Clothing. . 30III.Planning . 30IV.Unattended Operations . 30V.Fume Hood . 30VI.Proper Use of Fume Hoods . 31VII.Storage of Chemicals in the Laboratory . 31VIII.Working With Allergens . 31IX.Working With Embryotoxins . 31X.Working With Chemicals of Moderate Chronic or High Acute Toxicity . 33Alkylating Agents. . 33Acylating Agents. 33Organohalogen Compounds. 33Natural Products. 33Inorganic Compounds. . 34Aromatic Amines. . 34XI.Working With Substances of High Chronic Toxicity . 35References . 37F. SAFE PROCEDURES FOR SPECIFIC CLASSES OF HAZARDOUS MATERIALS . 38I.Flammable Solvents . 38General Characteristics. . 38Examples. . 39Use and Storage. . 39Health Hazards. 39Acute Health Effects. . 39Chronic Health Effects. . 40Flammable Groups Exhibiting Similar Health Effects. . 40First Aid. . 40Personal Protective Equipment. . 40II.Oxidizers . 41General Characteristics. . 41UTRGV Laboratory Safety Manual4

Examples. . 41Use and Storage. . 41Perchloric Acid. . 41Health Hazards. 42Acute Health Effects. . 42Chronic Health Effects. . 42First Aid. . 42Personal Protective Equipment. . 43III.Corrosives. 43General Characteristics. . 43Examples. . 43Use and Storage. . 43Hydrofluoric Acid. . 44Health Hazards. 44Acute Health Effects. . 45Chronic Health Effects. . 45First Aid. . 45Personal Protective Equipment. . 45IV.Reactives . 46General Characteristics. . 46Polymerization Reactions. 46Water Reactive Materials. . 46Pyrophorics. . 46Peroxide-Forming Materials. . 46Other Shock-Sensitive Materials. . 47Use and Storage. . 47Water Reactive Materials. . 47Pyrophorics. . 47Peroxide-Forming Materials. . 48Other Shock-Sensitive Materials. . 48Health Hazards. 48First Aid. . 48Personal Protective Equipment. . 49V.Compressed Gas Cylinders . 49General Characteristics. . 49Use and Storage. . 49UTRGV Laboratory Safety Manual5

Purchase Policy. 50References . 51G. BIOLOGICAL HAZARDS AND CONTROL . 52I.Recommended Laboratory Practices to Prevent Exposure . 52How to Prevent Contact with Skin or Mucous Membranes. . 52How to Prevent Ingestion. . 52How to Prevent Inhalation. . 52How to Prevent Inoculation. . 53II.Biosafety Levels . 53BSL1. . 54Examples. . 54Standard Practices. 54Special Practices. . 54Safety Equipment (Primary Barriers). . 55Laboratory Facilities (Secondary Barriers). . 55BSL2. . 55Examples. . 55Special Practices. . 55Safety Equipment (Primary Barriers). . 57Laboratory Facilities (Secondary Barriers). . 57BSL3. . 57Examples. . 58Special Practices. . 58Safety Equipment (Primary Barriers). . 58Laboratory Facilities (Secondary Barriers). . 59III.Laboratory Equipment . 60Biological Safety Cabinet. . 60Classes. . 60Proper Use. . 60Certification. . 61Clean Bench. . 61Pipetting Device. . 61Centrifuges, Sonicators, Homogenizers and Blenders. . 61Centrifuge. . 62Sonicators, Homogenizers and Blenders. . 62IV.Personal Protective Equipment . 62UTRGV Laboratory Safety Manual6

V.Waste Disposal . 62VI.Bloodborne Pathogens . 63Universal Precautions. 63Working with Animals. . 64VII.Emergency Procedures. 64BSL1. . 64BSL2. . 64BSL3. . 65References. . 65UTRGV Laboratory Safety Manual7

Laboratory SafetyMarch 2016A. INTRODUCTIONNote to Users of This Laboratory Safety ManualThe Department of Environmental Health, Safety and Risk Management (EHSRM) hasprepared this manual to ensure that laboratory activities are conducted in compliance withany applicable rules or regulations and best practices applicable to a laboratory environment.The health and safety policy of The University is to take every reasonable precaution toprotect the health and safety of faculty, staff, students, volunteers and the public. Mandatorysafety standards, as interpreted by the requirements and policies stated in this manual applyto faculty, staff, researchers, students, and volunteers engaged in laboratory operationsinvolving the utilization of chemical products and in performing common laboratoryprocedures.This manual includes information concerning safe laboratory practices, the use of personalprotective equipment, emergency procedures, use and storage of chemicals, and the propermethods of waste disposal. This manual covers safety practices for any area containingchemical, biological and/or radiological hazards and/or waste. This manual also covershazard communication and incident response. This information is intended to help those inthe laboratory minimize hazards to themselves and their colleagues.In view of the wide variety of chemical products handled in laboratories, it should notbe assumed that the precautions and requirements stated in this manual are allinclusive. Faculty, researchers, and students are expected to learn about the hazardsof chemical products before handling them. It is expected that the local departmentalChemical Hygiene Officers and Principal Investigators will append to this manual anysupplementary information pertinent to their specific areas.Throughout this manual the terms "employee", "laboratory worker", and "laboratorypersonnel" are used interchangeably and are intended to include students working in alaboratory, unless the context indicates otherwise.Please contact EHSRM Edinburg Campus: 956-665-3690 or Brownsville Campus: 956882-5929 for more information or for assistance.UTRGV Laboratory Safety Manual8

B. RESPONSIBILITIESEach individual conducting chemical reactions, using chemical materials, or performinglaboratory procedures is required to have proper training in the safe handling and disposal ofall materials he or she uses. Each is responsible for conducting activities in a manner that willnot endanger him- or herself and each must comply with the applicable requirements of stateand federal law as well as with University policies and procedures described in this manual.Oversight responsibility for ensuring that laboratory activities involving hazardous chemicalsconform to prescribed standards is assigned as follows:I.University-Wide Safety ResponsibilitiesThe PresidentThe UTRGV President has ultimate responsibility for safety within the institution.Environmental Health, Safety and Risk Management Provides hazard communication and laboratory safety training.Conducts periodic and unannounced laboratory inspections to assure compliance withfederal, state and local regulations, as well as the policies and procedures contained inthis manual and those contained in any supplementary information developed in thecollege in response to specific activities or areas of research.Undertakes necessary enforcement actions to insure full compliance with all institutionalsafety policies, up to and including independent authority to shut down laboratories forviolations of these policies. Approval of the Dean is not required.Provides for the proper disposal of all regulated waste streams generated in thelaboratories.Provides limited hazardous material spill response services.Reviews laboratory construction, modification and renovation plans for safety design.In conjunction with Campus Facilities Operations, oversees fume hood surveys andtesting.Oversees biological safety cabinet testing.Performs exposure monitoring upon request to determine if the permissible exposure limitor action level has been exceeded. Notification shall be provided to laboratory supervisor.Provides guidance for maintaining compliance with federal, state, and local regulations,as well as the procedures stated in this manual.Conducts laboratory safety evaluations when requested by laboratory supervisors ordepartment chairs.Provides assistance in obtaining personal protective equipment.Maintains copies of medical consultations and examinations for possible exposures fromhazardous chemicals.Campus Facilities Operations and Utilities Maintains facilities and facility-related safety systems to assure continuous operation oflaboratories.UTRGV Laboratory Safety Manual9

II.In conjunction with EHSRM, oversees fume hood testing.Oversees certification of fume hoods.Departmental Safety ResponsibilitiesDean Has the responsibility to insure the safe operation of all laboratories and other sites in theUniversity where chemicals are used or laboratory procedures are conducted.Will ensure compliance with the policies and procedures contained in this manual andthose contained in any supplementary information developed within the University inresponse to specific activities or areas of research.Has independent enforcement authority to close a laboratory for safety violations.Approval of the Director of EHSRM is not required.Department Chairs and Directors Oversee chemical and biological hygiene within departmental laboratories by ensuringthat supervisory personnel reporting to them assume their responsibilities for adheringto all safety policies, regulations and procedures.Complete and update annual inventories of hazardous chemicals as required by theTexas Hazard Communication Act and the UTRGV Department of EnvironmentalHealth, Safety and Risk Management’s Laboratory Safety Program.If authorized by the dean, appoint and transfer appropriate enforcement authority to aDepartmental Chemical Hygiene Officer (CHO). The Department Chair or Directorassumes all the responsibilities of the CHO when there is not a specifiedChemical Hygiene Officer.Chemical Hygiene Officer Advises the Dean, Department Chair, or Director on matters of chemical safetypolicies and practices.Works with employees to develop and implement the chemical hygiene policies andpractices outlined in this manual and those contained in any supplementaryinformation developed in the college in response to specific activities or areas ofresearch.Monitors compliance with policies and procedures for the procurement, safe use,and proper disposal of chemicals.Investigates and retains records of accidents involving hazardous materials.Conducts informational and general training sessions.Maintains a resource file of references and publications on safety matters.Assists laboratory supervisors and principal investigators in writing StandardOperating Procedures (SOPs) pertinent to their needs.Ensures that action is taken to correct laboratory practices and conditions that mayresult in the release of hazardous materials.Ensures that action is taken to correct laboratory practices and conditions identifiedas unacceptable on laboratory safety self-evaluations and safety inspections.UTRGV Laboratory Safety Manual10

Principal Investigator/Laboratory Supervisor Designs and conducts laboratory processes and operations to assure that employeeexposure to risk conforms to the policies, procedures and objectives contained in thismanual and those contained in any supplementary information developed in theUniversity in response to specific activities or areas of research.Monitors the procurement, safe use, and proper disposal of chemicals.Writes Standard Operating Procedures and other information relevant to lab processes intheir specific areas as needed to supplement those contained in this manual.Instructs employees on the contents of this manual, its appendices, and anysupplements, and the location of the manual and related materials within the workplace.Takes all reasonable precautions to protect the safety and health of laboratory workersand the environment.Schedules services for hazardous waste disposal and oversees the handling ofhazardous waste pending proper disposal.Conducts regular laboratory safety self-evaluations.Completes and updates annual laboratory chemical inventories in accordance with theinstructions and schedule provided by the Department of Environmental Health, Safetyand Risk Management.Informs employees of the permissible exposure limits for the hazardous chemicals listedon inventories and the signs and symptoms associated with exposures to thesechemicals.Provides site specific training on laboratory hazards as described in the University ofTexas-Rio Grande Valley Laboratory Safety Manual.Determines the required levels of personal protective equipment, fire extinguishers, fumehoods, flammable liquid storage cabinets, biological safety cabinets, eye washes, safetyshowers, and spill cleanup kits. Assures that all required equipment is available and inworking order and that appropriate training for each item has been provided.Has readily available a current copy of a Safety Data Sheet for all hazardous chemicals inthe laboratory.Posts emergency telephone numbers on the outside of each laboratory door and by alltelephones in the area.Reports to EHSRM if there is reason to believe that exposure levels for a hazardouschemical exceed the action level or the permissible exposure limits and document theincident.Forwards documentation on laboratory accidents and exposures to EHSRM.Provides for the safety of visitors. Laboratory Employee and Other Personnel Maintains a thorough understanding of and follows the laboratory policies andprocedures in this manual and those contained in any supplementary informationdeveloped for UTRGV in response to specific activities or areas of research for allprocesses using chemical materials.Uses and maintains personal protective equipment (i.e. lab coat, chemical splashgoggles, face shield, respiratory protection, and gloves) as mandated in this manual forlaboratories.Uses flammable liquid storage cabinets, acid storage cabinets, biological safetycabinets, fume hoods, and other laboratory safety equipment provided.Informs supervisor immediately of any laboratory safety equipment that is needed butnot available or that is not in good working order.UTRGV Laboratory Safety Manual11

Informs supervisor immediately of exposure symptoms, accidents, or chemical releasesand documents incident.Successfully completes all applicable training sessions.UTRGV Laboratory Safety Manual12

C. EMERGENCY PROCEDURESAll accidents, hazardous material spills or oth

UTRGV Laboratory Safety Manual 8 Laboratory Safety March 2016 A. INTRODUCTION Note to Users of This Laboratory Safety Manual The Department of Environmental Health, Safety and Risk Management (EHSRM) has prepared this manual to ensure that laboratory activities are conducted in compliance with

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