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STUDY & EVALUATION SCHEMEB. TECH. Biotechnology(w.e.f. – January 2017)2nd YearIV SemesterEvaluation SchemeCourseSl. Category SubjectNo.CodePeriodsName of the SubjectSessional BE-207 Immunology3104251540601002.DCBE-208 Enzymology3104251540601003.DCBE-209 Molecular Biology3104251540601004.DCBE-210 Introduction to Bioinformatics210** 25* 15* 40*60*100*5.ESAME-222 Heat Transfer 40100BE-211 Immunology Lab004230306040100BE-213 Plant Biochemistry6.DEBE-214 Clinical BiochemistryApplied Thermodynamics forBioengineersES -202/ Disaster Management/CS-203 Cyber Law & Information SecurityBE-2157.8.ESADC9.DCBE-212 Molecular Biology Lab00423030604010010.ESAME-224 Heat Transfer Operation Lab00423030604010017 7 12 27 245 195 440460900Total** A non-credit foundation course. Candidate has to pass the course by securing at least 50 % marks up tosecond semesterL – LectureT – TutorialP – PracticalC – CreditsCT – Class TestTA – TeacherAssessmentSessional Total (CA) Class Test Teacher AssessmentBS – Basic SciencesHM – HumanitiesDE – Departmental ElectiveCourses)DC – Departmental CoreOE – Open ElectiveESA– Engineering Sciences & Arts (Foundation Course & Engineering

IMMUNOLOGYBE-207(w.e.f. – January 2017)LTPC3104Unit I[8]THE IMMUNE SYSTEM: Introduction, Characteristics of innate and adaptive immunity,Humoral and cell mediated immunity, Cells and molecules of immune system, Primary andSecondary lymphoid organs, antigens, antigenicity and immunogenicity, Factor affecting theimmunogenicity.Unit II[8]IHUMORAL IMMUNITY: B-Iymphocytes and their activation, structure and function ofimmunoglobulin, immunoglobulin classes and subclasses, Clonal selection of antibodyproduction, polyclonal and monoclonal antibody and diagnosis, idiotypic antibodies, Structureand function of MHC molecules.Unit III[8]CELLULLAR IMMUNOLOGY: Thymus derived Lymphocytes (T Cells) their classification,antigen presenting cells (APC), Exogenous and endogenous pathways of antigen processing andpresentation, cytokines and their role in immune response macrophage activation and granulomaformation, immunosuppression, immune tolerence. Structure, function and application ofcytokines.Unit IV[8]ANTIGEN AND ANTIBODY REACTION: serological techniques-ELISA, RIA andImmunoblotting.IMMUNITY TO INFECTION: Hypersensitivity reactions, complement system adjuvants,dose of antigens, types of vaccine preparation, Immunity against infectious diseases (virus,bacteria and protozoan).Unit V[8]TRANSPLANTATION: Graft rejection, evidence and mechanisms of graft rejection,prevention of graft rejection, immunosuppressive drugs, HLA and disease, mechanisms ofimmunity to tumour antigens.AUTO IMMUNITY: Auto antibodies in humans, pathogenic mechanisms, experimental modelsof autoimmune disease treatment of auto immune disorders and cancer.Books recommended:1. Essential Immunology- I. M. Roitt, 10th ed., Blackwell Scientific Publications, Oxford- 4th ed.2. Kuby’s Immunology- Murry, 5th ed.3. Ivan M. Roitt, J. Brostoff and David K. Male. Immunology, Glower Medical Publishers,London.4. Basic and Clinical Immunology, 5th ed., D. P. Sites, J. D. Stobo, H. H. Fudenberg and J. V.Wells. Longe Medical Publications, Singapore.

ENZYMOLOGYBE-208(w.e.f. – January 2017)LTPC3104Unit I[8]Introduction, classification and nomenclature of enzymes. Active site, isoenzymes,coenzymes, cofactors, turn over number, enzyme specificity, enzyme activity,specific activity, multienzyme complexes, Physico-chemical characteristic ofenzymes. Effect of pH, temperature and substrate concentration on enzyme actvity.Unit II[8]Enzyme kinetics, derivation of Michelis Menten equation, measurement of Km andVmax, Enzyme inhibition, kinetics of competitive, non-competitive and uncompetitive inhibition of enzymes. Effect of pH, temperature and substrateconcentration on enzyme kinetics. Allosteric enzymes and their kinetics.Unit III[8]Introduction to industrial enzymes: Topoisomerases, chymotrypsin, glyceraldehyde3- phosphate dehydrogenase, lysozyme, carboxypeptidase, ribonuclease, lactasesand ribozyme and their mechanism of action.Unit IV[8]Molecular folding and defolding of enzymes. Stability of enzymes. Methods ofenzyme immobilizations and their applications.Unit V[8]Isolation, purification, and characterization of the enzymes from microorganisms.Intra cellular and extra cellular enzymes, Methods for lysis of plant, animal andmicrobial cell. Use of detergents in isolation of membrane proteins. Industrial,diagnosis and therapeutic application of enzymes.Books recommended:1. Methods in enzymology series2. Murray Moo-Young- Comprhensive Biotechnology, II & IV th Vol.3. Biochemistry- D. J. Voet & J. G. Voet, 6th ed.4. Methods in Enzymology- A series.5. Enzyme Kinetics- Hans Bisswanger, Wiley Publication6. Biocatelysis: Fundamentals and Application- A.S. Bommarius et al., Wiley Publication

MOLECULAR BIOLOGYBE-209(w.e.f. – January 2017)LTPC3104Unit I[8]Central Dogma, Mutation: Spontaneous, induced; Chemical and physical mutagens; Non sensemutation; Missense mutation; Frame shift mutation; Suppressor mutation; Different methods ofDNA repair and SOS response; transposable elements: retrotransposon, mechanism oftransposition.Unit II[8]DNA replication in prokaryotes: Roles of DnaA, Helicase, HD protein, Primase, DNA gyrase,Topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase, Fidelity, Regulation;DNA replication in eukaryotes: Autonomously replicating sequence, Origin recognitioncomplex, Minichromosome maintenance proteins, DNA polymerases α, δ, ε, Nucleases, DNAligase, Telomeres, Regulation; Mitochondrial DNA replication; Reverse transcriptase.Unit III[8]Transcription in prokaryotes: Outline of process, Promoter, RNA polymerase; Transcription ineukaryotes: Outline of process, Promoters, Enhancers, RNA polymerase I, II, III; Posttranscriptional modifications: End modifications (Addition of 5 cap and 3‟ Poly A tail inmRNA), RNA splicing - Self splicing and Spliceosome mediated splicing, Cutting events oraction of ribonucleases, Covalent modifications, RNA editing, Alternative splicing.Unit IV[8]Genetic code, Wobble hypothesis; Translation in prokaryotes and eukaryotes: Outline of process,Types of RNA, Structure of ribosome, Aminoacyl RNA transferase.Unit V[8]Post - translational processing: Intein splicing, Chemical modification, Proteolytic cleavage,Zymogen activation; Regulation of gene expression: Operon concept: Lac and Trp.Books recommended:1. Albert B, Bray Denis et al.: Molecular Biology of The Cell, latest ed.2. Watson, Hopkin, Roberts et al.: Molecular Biology of the Gene, 4 th ed3. Genetics – Strickberger, 2 nd .4. Microbial Genetics- D. Frifielder5. Baltimore- Molecular Biology of the Cell6. Benjamin Levin - Genes VIII, 8 th ed.7. Advance Genetics by G.S. Miglani, Narosa Publishing House

INTRODUCTION TO BIOINFORMATICSBE-210(w.e.f. January 2017)LT PC2 10 0Unit I[08]Definition of Bioinformatics, Biological databases: Nucleotide databases (e.g., GenBank,EMBL, and DDBJ), Biological search engines (e.g., Entrez, SRS, and ARSA).Unit II[08]Biological databases: Protein databases- Primary protein databases (e.g., SwissProt, Tr-EMBL,and PIR), Secondary protein databases (e.g., PROSITE, PRINTS, IDENTIFY, BLOCK, andPFAM), Composite database (e.g., OWL, and NRDB).Unit III[08]Biological databases: Literature databases (e.g., PubMed and PubChem), Biological file formats(e.g., GenPept/GenBank, FASTA, and EMBL), Applications of Bioinformatics.Practicals[08]1. Retrieval of nucleotide sequences from different nucleotide databases and their analysis.2. Retrieval of protein sequences from different protein databases and their analysis.3. Retrieval of small chemical compounds from PubChem compound databases and theiranalysis.4. Briefing about retrieval of scientific articles from PubMed database.Books recommended:1. D. W. Mount: Bioinformatics-sequence and genome analysis, Cold Spring Harbor lab Press.2. Jin Xiong: Essential Bioinformatics, Cambridge University press.

HEAT TRANSFER OPERATIONSME-222(w.e.f.- January 2017)LTPC210 3Unit I[8]Introduction to heat transfer and general concepts of heat transfer by conduction,convection and radiation. Conduction: Basic concepts of conduction in solids,liquids and gases, steady state temperature fields and one dimensional conductionwithout heat generation e.g. through plane walls, Cylindrical, Spherical surface andComposite layers etc. Insulation materials, critical and optimum insulationthickness. Extended surface, fines and their practical applications. Introduction tounsteady state heat transfer.Unit II[8]Convection: Fundamentals of convection, Basic concepts and definitions, Naturaland forced convection, Introduction to hydrodynamics and thermal boundarylayers, Dimensional analysis of force and free convection,Heat transfer with phase change: Condensation of pure and mixed vapors, filmwise and drop wise condensation, heat transfer in boiling liquidsUnit III[8]Heat Transfer equipment: Classification, principles and design criteria, types ofexchanger’s viz. double pipe, shell and tube, plate type, Fouling factors; Overallheat transfer coefficient; Logarithmic mean temperature difference (LMTD)method. Furnaces and their classification and applicationsUnit IV[8]Radiation & Evaporation: Basic laws of heat transfer by radiation, black body andgray body concepts, view factors, Krichoff‟s law, Black-body radiation exchange,solar radiations, Combined Heat Transfer Coefficient by convection and radiation,Elementary principles, types of evaporators, Single and multiple effect evaporatorsBooks Recommended:1. Holman, J. P. "Heat Transfer" McGraw Hill Books Co.2. Mc Adams W.H. "Heat Transmision" McGraw Hill Books Co.3. Kern D.Q. " Process Heat Transfer" McGraw Hill Books Co.4. Bedger W.L. and Bancharo J. T. "Introduction to Chemical Engineering" TataMcGraw publication House.5. Heat Transfer; R. Yadav, Central Publishing House, Allahabad.

DISASTER MANAGEMENTES-202(w.e.f.- July 2016)LTPC21 0 3Objective:The objective of this course is to familiarize the student with basic management principlesrelating to disaster management and mitigation techniques.Units I[8]Concept of disaster management. Types of disaster and their impact: Natural and Man- Made,Like- Earthquakes, Floods, Tsunami, Droughts, Cyclones, Avalanches, Forest Fire, Terrorismrelated Disaster etc. Assessment of human and Economic losses.Units II[8]Impact of extensive Industrialization. Impact of Global Warming and EnvironmentalDegradation. National and global disasters.Units III[8]National policy for disaster Management, Elementary Knowledge of the Disaster ManagementAct 2005. Types of Responses: Central, State, District Level, Peoples Community participationin Disaster Management. Post-Disaster Management and Rehabilitation measures.Unit IV[8]Capacity Building for meeting disasters. Long term measures for preventions of disasters.Mitigation technique/strategies: Early warning systems, Data sharing at national andinternational level.References:1. Disaster management by Dr. V.K Sethi2. The great Sumatra Earthquakes and Indian Ocean tsunami of December 2004- the effects ofmain land India and in the Andaman and Nicobar Island, published by IIT Kanpur.3. Environmental managaenet by Dr. Shakeel Ahmad4. Hazards, disasters and your community, ministry of home affairs.

CYBER LAW AND INFORMATION SECURITYCS-203(w.e.f.- January 2017)LTPC21 0 3Unit1[8]Fundamentals of Cyber Law: Jurisprudence of Cyber Law, Object and Scope of the IT Act 2000,Introduction to Indian Cyber Law, Unicitral Model Law, ISP Guideline. Intellectual propertyissues and cyber space, Indian perspective, Overview of Intellectual property related legislationin India, Patent, Copy Right, Trademark law, Law related to semiconductor layout & design.Unit 2[8]E - Commerce: Security Threats to E - Commerce, Virtual Organization, Business Transactionson Web, E-Governance and EDI, Concepts in Electronics payment systems, E-Cash,Credit/Debit Cards, E- Agreement, Legal recognition of electronic and digital records, ECommerce Issues of privacy, Wireless Computing- Security challenges in Mobile devices.Digital Signatures - Technical issues, legal issues, Electronic Records, Digital Contracts,Requirements of Digital Signature System.Unit 3[8]Investigation and Ethics: Cyber Crime, Cyber jurisdiction, Cyber crime and evidence act,Treatment of different countries of cyber crime, Ethical issues in data and software privacy,Plagiarism, Pornography, Tampering computer documents, Data privacy and protection, DomainName System, Software piracy, Issues in ethical hacking. Internet security treats: Hacking,Cracking, Sneaking, Viruses, Trojan horse, Malicious Code & logic bombs. Introduction tobiometric security and its challenges, Finger prints. Cyber crime forensic: CASE STUDY inCyber Crime.Unit 4[8]Information security- Information Systems and its Importance, Role of Security in Internet andWeb Services, Principles of Information Security, Classification of Threats and attacks, SecurityChallenges, Security Implication for organizations, Security services - Authentication,Confidentiality, Integrity, Availability and other terms in Information Security, InformationClassification and their Roles. Introduction to Cryptography, Issues in Documents Security,Keys: Public Key, Private Key, Firewalls, Basic Concepts of Network Security, Perimeters ofNetwork protection & Network attack, Need of Intrusion Monitoring and Detection.References:1. Harish Chander “Cyber Law and IT Protection” , PHI Publication, New Delhi2. Merkov, Breithaupt “ Information Security”, Pearson Education3. “Cyber Law in India” - Farooq Ahmad-Pioneer books.4. K. K. Singh, Akansha Singh “Information Security and Cyber law”, Umesh Publication, Delhi

IMMUNOLOGY LABBE-211(w.e.f.- January 2017)L TPC0 0 4 21. Isolation, purification and identification of Antibodies.2. Double diffusion techniques for identification of Antigen-Antibodysamples.3. Immunoelectrophoresis techniques.4. ELISA (Enzyme Linked Immunosorbent Assay)5. Immunoblotting Using ELISA-dot6. Western blot technique (Demonstration)7. ABO blood group typing8. RBC and WBC count by Heamocytometer

MOLECULAR BIOLOGY LABBE-212(w.e.f.- January 2017)L T PC0 0 4 21.Estimation of DNA content in the given sample by diphenylamine method.2.Estimation of RNA content by the Orcinol method.3.Determination of Tm of DNA and RNA.4.Isolation of plasmid DNA.5.Isolation of bacterial genomic DNA6.Isolation of plant DNA.7.Visualization of DNA by Agarose Gel electrophoresis.

HEAT TRANSFER OPERATION LABME-224(w.e.f. – January 2017)LTPC0 04 21. To determine the Steffen’s Boltzmann Constant2. To determine the surface heat transfer coefficient for a vertical tube losing heatby natural Convection.3. To find the heat transfer coefficient of forced convection in internal pipe flow4. To determine the efficiency and effectiveness of a fin and study the temperaturedistribution in a simple pin fin.5. To study heat transfer through the pipe of different metals.6. To determine the LMTD, overall heat transfer coefficient and effectiveness of aheat Exchanger working in parallel flow mode.7. To determine the LMTD, overall heat transfer coefficient and effectiveness of aheat Exchanger working in counter flow mode.8. To determine the overall heat transfer coefficient for the composite wall and tocompare the same with that calculated from the equation.

PLANT BIOCHEMISTRYBE-213(w.e.f. January 2017)LTPC2103Unit 1[8]Photosynthesis – Photosynthetic apparatus, pigments of photosynthesis, role of carotenoids,photosystems I and II, their location; Hill reaction, photosynthetic electron transport andgeneration of NADPH & ATP, cyclic and non-cyclic photophosphorylations, complexesassociated with thylakoid membranes; light harvesting complexes, path of carbon inphotosynthesis – C3 and C4 pathway of carbon reduction and its regulation, Photorespiration.Unit 2[8]Nitrate assimilation, structural features of nitrate reductase and nitrite reductase, incorporationof ammonia into organic compounds, regulation of nitrate assimilation.Unit 3[8]Plant Hormones – Biosynthesis of growth regulating substances- auxins, cytokinins, gibberllicand abscisic acids.Unit 4[8]Stress metabolism in plants – Environmental stresses, salinity, water stress, heat, chilling,anaerobiosis, pathogenesis, heavy metals, radiations and their impact on plant growth andmetabolism, criteria of stress tolerance.Reference Books1.2.3.4.Plant Biochemistry by P. M. Dey and J. B. Harborne, Harcourt Aria PTE Ltd. Singapore.Plant Physiology by Salinbury.Plant Physiology by Davin.Lehninger Biochemistry

CLINICAL BIOCHEMISTRYBE-214(w.e.f. January 2017)LTPC2103Unit I Hydrogen ion and electrolyte homeostasis[8]Hydrogen ion homeostasis: Factors regulating blood pH- buffers, respiratory and renalregulation. Acid-base balance- causes, biochemical findings and management of metabolic andrespiratory acidosis and alkalosis. Water, sodium and potassium homeostasis: Distribution ofwater and electrolytes in the ECF and ICF.Unit II Inborn errors of metabolism[8]Disorders of amino acid metabolism- aminoaciduria, phenylketonuria, alkaptonuria, cystinuria,and maple syrup urine disease. Disorders of carbohydrate metabolism- glycogen storagediseases, galactosemia, fructose intolerance Disorders of purine, pyrimidine and porphyrinmetabolism- Hyperuricemia and gout, Hypouricemia. Disorders of lipid metabolism- lipidstorage diseases, fatty liver.Unit III Kidney and nutritional disorders[8]Kidney function tests: Glomerular and tubular function tests. Abnormal constituents of urine.Pathogenesis, biochemical findings and management of glomerulonephritis, renal failure,nephrotic syndrome Protein energy malnutrition- Marasmus and Kwashiorkor.Unit IV Liver and gastrointestinal disorders, Diabetes, and Cancer[8]Liver function tests (excretory, synthetic, detoxification and metabolic). Plasma enzymes in liverdisease. Jaundice- neonatal. Gastric function tests. Pathogenesis, biochemical findings andmanagement of peptic ulcer and gastritis. Pancreatic and intestinal function tests. Classificationof diabetes. Metabolic abnormalities – glycosuria. Acute and long term complications, Diagnosisand management. Cancer: Differences between benign and malignant tumours. Morphologicaland biochemical changes in tumour cells. Tumour markers (AFP, CEA, hCG only).Books for Reference:1. Principles of Internal Medicine. Harrison’s Vol 1 & 2, 14th edition McGraw Hill, 1998.2. Harper’s Biochemistry McGraw Hill, 27th ed, 2006.3. Biochemistry – A case oriented approach. Montgomery et al. Mosby. 1990 5th editon.4. Clinical Chemistry – Principles, procedures, correlations – Bishop, Lippincott, 2005, 5thedition.5. Guyton and Hall. Textbook of Medical Physiology 12 ed 2011

APPLIED THERMODYNAMICS FOR BIOENGINEERSBE-215(w.e.f- January 2017)LT PC2 1 03UNIT I08First Law of thermodynamics, a generalized balance equation and conserved quantities,Volumetric properties of fluids exhibiting non ideal behavior; residual properties; estimation ofthermodynamic properties using equations of state; calculations involving actual propertyexchanges.UNIT II08Partial molar properties; concepts of chemical potential and fugacity; ideal and non-idealsolutions; concepts and applications of excess properties of mixtures; activity coefficient;composition models.UNIT III08Equilibrium criteria for homogeneous chemical reactions; evaluation of equilibrium constant;effect of temperature and pressure on equilibrium constant; calculation of equilibrium conversionand yields for single and multiple reactions.UNIT IV08Thermodynamics of microbial growth stoichiometry thermodynamics of maintenance,Calculation of the Operational Stoichiometry of a growth process at Different growth rates,Including Heat using the Herbert –Pirt Relation for Electron Donor, thermodynamics andstoichiometry of Product Formation.TEXT BOOKS:1. Smith J.M., Van Ness H.C., and Abbot M.M. “Introduction to Chemical EngineeringThermodynamics”, 6th Edition. Tata McGraw-Hill, 2003.2. Narayanan K.V. “A Text Book of Chemical Engineering Thermodynamics”,PHI,2003.3. Christiana D. Smolke, “ The Metabolic Pathway Engineering Handbook Fundamentals”,CRC Press Taylor & Francis Group, 2010.REFERENCE:1. Sandler S.I. “Chemical and Engineering Thermodynamics”, John Wiley,1989.

DC BE-208 Enzymology 3 1 0 4 25 15 40 60 100 3. DC BE-209 Molecular Biology 3 1 0 4 25 15 40 60 100 4. DC BE-210 Introduction to Bioinformatics 2 1 0 ** 25* 15* 40* 60* 100* 5. ESA ME-222 Heat Transfer Operations 2 1 0 3 25 15 40 60 100 . Methods of enzyme immobilizations and their applications.

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