PowerEase 500 Power Supply - Thermo Fisher Scientific

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USER GUIDEPowerEase 500 Power SupplyA programmable power supply for electrophoresisCatalog Numbers EI8600, EI8700, and EI8675Document Part Number A12162Publication Number MAN0000738Revision 9.0For Research Use Only. Not for use in diagnostic procedures.

Table of ContentsWarning. 4Kit Contents . 5Product Specifications . 6Front and Rear View of PowerEase 500 . 7Introduction . 10Description of PowerEase 500 . 10Methods . 12Getting Started . 12Operational Modes . 13Using PowerEase 500. 14Printing . 21Custom Methods . 22Troubleshooting . 24Appendix . 25Instrument Symbols and Safety . 25Consignes De Securite . 26Sicherheits Anweisungen . 27Run Conditions for Gels . 28Repair, Maintenance, and Cleaning . 29Background Information on Voltage, Current, and Resistance . 31Accessory Products. 32Technical Support . 333

WarningFederalCommunicationsCommissionAdvisoryThis equipment has been tested and found to comply with the limits for a ClassA digital device, pursuant to part 15 of the FCC rules. These limits are designedto provide reasonable protection against harmful interference when theequipment is operated in a commercial environment. This equipmentgenerates, uses, and can radiate radio frequency energy and, if not installed andused in accordance with the instruction manual, may cause harmfulinterference to radio communications. Operation of this equipment in aresidential area is likely to cause harmful interference in which case the userwill be required to correct the interference at their expense.Changes or modifications not expressly approved by the party responsible forcompliance could void the user’s authority to operate the equipment.4

Kit ContentsTypes of KitsThis manual is supplied with the following kits:KitCatalog No.PowerEase 500 Power Supply (100/120 Vac 50/60 Hz)EI8600 Kit ComponentsPowerEase 500 Power Supply (220/240 Vac 50/60 Hz)EI8700PowerEase 500 Pre-Cast Gel Systemincludes PowerEase 500, Temperature Monitoring Probe,XCell SureLock Mini-Cell and XCell II Blot ModuleEI8675PowerEase 500 Power Supply contains:PowerEase 500 Power Supply1 eachInstruction Manual1 eachExtra Fuses2 eachPower Cord (U.S., Canada, Europe, Taiwan and Japan) 1 eachWarranty Card1 eachTemperature Probe (Optional)1 eachSee page 6 for specifications and detailed description of PowerEase 500 PowerSupply.NoteTo ensure safe, reliable operation, always operate the PowerEase 500 PowerSupply in accordance with the manufacturer’s instructions. Always wearprotective gloves and safety glasses when working in a laboratory environment.See safety information on pages 25–27.Warranty information is provided on page 33.5

Product SpecificationsSpecificationsInput Power100–120 VAC:100–120V, 50/60 Hz, 1.2 A at maximum loadFuse:One 1A/250V/AGC fast blow fuse220–240 VAC:220–240VAC, 50/60 Hz, 0.5 A at maximum loadFuses:Two 1A/250V/5 20 mm fast blow fusesOutput Range1–500 V (minimum step size 1 V)1–500 mA (minimum step size 1 mA)0.1–50 W (minimum step size 0.1 W)Automatic crossover on reaching set limits.AccuracyVoltage: 2% or 2 voltsCurrent: 2% or 2 mAWattage 2% or 0.2 wattDrift 1% in 8 hours after 30 minute warm-upwith constant supply voltageCircuit ProtectionOpen Circuit; Short Circuit; Thermal ProtectionOver Voltage, Current, WattageSafetyLoad DetectionThermal Shutoff (with optional temperature probe)Isolated Floating GroundMemory ControlNon-volatile, up to 24 hoursCustom Methods Stored to RAMComputer InterfaceRS232CDisplayLCD, view area 114 x 64 mm240 128 dots6Dimensions16.5 cm (w) 18 cm (h) 20.5 cm (d)Weight2.9 kg (6.4 lbs)OperatingEnvironmentalConditionsAmbient temperature 4–30 C, 80% relativehumidity, altitude 2000 meters, indoor use only,pollution degree 2

Front and Rear View of PowerEase 500Front View ofPowerEase 50018RSTA TPOWER5HIGH VOLTAGE7500S TO P6seSELECTrEPowea 4TEMP231.LCD Screen2.Power Output Jacks, Positive (Red)3.Power Output Jacks, Negative (Black)4.Temperature Probe Jack5.Functional Keys (Power, Start, Stop)6.Directional Keys (Up, Down, Left, Right)7.High Voltage LED8.Power LEDContinued on next page7

Front and Rear View of PowerEase 500, ContinuedRear View ofPowerEase rtPower Entry Modulewith On/Offmain power switchContinued on next page8

Front and Rear View of PowerEase 500, ContinuedKey PadPower button secondary On/Off switch for the high voltage components, fan, and LCDscreen.Start button used to start a selected method program.Stop button used to end the selected method and to backup to a previous screen.SELECTSelect button used to select a menu choice and to move forward to the next applicablescreen.Left directional button for moving left around the menu.Right directional button for moving right around the menu.Up directional button for moving up around the menu and to adjust numerical andcharacter values (higher).Down direction button for moving down around the menu and to adjust numerical andcharacter values (lower).9

IntroductionDescription of PowerEase 500ProductDescriptionThe PowerEase 500 Power Supply is a microprocessor-controlled power supplyfor electrophoresis of pre-cast and hand-poured mini-gels. The power supply isdesigned to simplify electrophoresis by combining high performance andprogramming flexibility with ease of use when running mini-gel and blottingapplications.The PowerEase 500 Power Supply is designed around the single gel concept:you need to think only about the electrical parameters and limits for a single gel.This manual describes the setup and operation of PowerEase 500 Power Supplyincluding important information on safety and maintaining the unit.Features ofPowerEase 500The important features of PowerEase 500 Power Supply are: Large LCD display allows electrical parameters to be displayed in eithergraphical or numerical formats Eight pre-programmed methods for running and blotting Novex pre-castmini-gels Four custom methods for running your custom applications Simple three step operating procedure to start running or blotting Novex pre-cast gels Microprocessor control provides highly accurate electrical outputs resultingin exceptional blotting capability Parallel port for printing experimental results to most printers Optional Temperature Probe to continuously monitor the buffer temperatureand shut the unit down in the event of overheating Two sets of output jacks allows electrophoresis of multiple mini-cell units inconstant voltage, current, or wattsContinued on next page10

Description of PowerEase 500, ContinuedFlowchartThe following flowchart describes the various screens displayed on thePowerEase 500 Power Supply and the keypad buttons (Start, Stop or Select)used to navigate through the screens.Power ONStart-Up ScreenStart/SelectGel Method ScreenSelectGel Quantity ScreenStartSelectMethod Edit ScreenStartMethod Run Large DisplayMethod Run Large DisplayStopPrevious ScreenStopPrevious ScreenSelect, onceMethod Run Graphic DisplaySelect, twiceSelectMethod Run/Edit ScreenSelect, twiceMethod Run Graphic DisplaySelect, onceMethod Run Large Display11

MethodsGetting StartedInstallingPowerEase 500Installing anOptional Printer1.Place the PowerEase 500 Power Supply on a level bench. Keep the areaaround the power supply clear to ensure proper ventilation of the unit.2.Position the unit properly such that the On-Off switch and the power cordattachment module located on the rear of the unit are easily accessible.3.Check the label located near the power entry module to ensure that the unitis of the proper local voltage. Attach the power cord to the power entrymodule. Use only properly grounded AC outlets.The PowerEase 500 Power Supply has a DB25 parallel connector located on therear of the unit. This allows the PowerEase 500 Power Supply to print a reportof your electrophoresis results in ASCII format to most commonly availableprinters.Use the following steps to install a printer:1.Using a parallel printer cable, connect the male DB25 end of the printer cableinto the port marked “Printer” located on the rear of the PowerEase 500Power Supply.2.Connect the opposite end of the cable into the appropriate connector on theprinter. Refer the user manual of your printer for instructions on connectingthe cable to the printer.RS232 PortThe RS232 Port is designed to be connected to a computer during diagnosticsand/or servicing by qualified field service engineers only.Installing anOptionalTemperatureProbeIf you have ordered the Temperature Monitoring Probe, use the followinginstructions to install the probe:121.Unpack the Temperature Monitoring Probe and inspect for any possibledamage to the wire or connector which may have occurred during shipping.2.Plug the connector end of the Temperature Monitoring Probe into the portlocated at the front lower right corner of the PowerEase 500 Power Supplymarked “TEMP”.3.Place the opposite end of the Temperature Monitoring Probe with the bluecolored bead into an electrophoresis cell buffer chamber to displaytemperature on the LCD screen of the PowerEase 500 Power Supply. Youcan bent the wire, but avoid crimping or pinching the wire. Temperature willbe displayed at all times even when the PowerEase 500 Power Supply isstopped.

Operational ModesIntroductionThe PowerEase 500 Power Supply is capable of operating at limiting voltage,limiting current, and limiting power. We recommend operating the PowerEase 500 Power Supply at limiting voltage for most applications. See below for moredetails.Voltage LimitingThe recommended setting for operating the PowerEase 500 Power Supply isvoltage limited. For most electrophoresis methods, resistance increasesthroughout the run. Using voltage limiting provides the following advantages:Current Limiting Current and watts decrease throughout the run, providing a natural safetymargin. The same voltage setting can be used regardless of the number or thicknessof gels being electrophoresed.Discontinuous buffer systems and, to a lesser extent continuous systems,increase resistance during the run. If you use the current limiting setting on thePowerEase 500 Power Supply, the voltage will increase as resistance increasesto satisfy Ohm’s law (V IR, see page 21). If no voltage limit is set and a localfault condition occurs, such as a poor connection, very high local resistancemay cause the voltage to increase to a maximum of the power supply. This willlead to local overheating and damage to the electrophoresis cell or create unsafeconditions.When running under constant current conditions, set a voltage limit on thepower supply at or slightly above the maximum expected voltage.Wattage LimitingIf power is constant, voltage will increase and current will decrease during arun, but the total amount of heat generated by the system will remain constantthroughout the run. However, locally high resistance can cause a highproportion of the total heat to be generated over a small distance. This candamage the electrophoresis cell and/or gel(s).If operating at wattage limiting, set the voltage limiting to slightly above themaximum expected for the run.13

Using PowerEase 500IntroductionYou can operate the PowerEase 500 Power Supply immediately afterinstallation using three simple steps (see below). For programming customsettings, see page 22.StartingPowerEase 5001.Plug the power cord of the PowerEase 500 Power Supply into an availableelectrical outlet rated at the appropriate electrical values for the powersupply.2.Turn on the main power switch located on the rear of the PowerEase 500Power Supply (see page 8 for rear view of PowerEase 500 Power Supply).Always leave this switch in the ON position to keep the back-up battery fullycharged.3.Press the Power button located on the keypad (see page x for a figure of thekeypad). This Power button is a secondary power button and does notactually shut the instrument down, but shuts down the high voltage, fan,and LCD screen. When powered back up, the LCD screen will revert to thesame screen as when the secondary power was shut off.4.A Start-Up screen appears (see page 15).To avoid condensation, the main power switch located on the rear of the unitshould be left on between use during operation in a cold room or in highhumidity environments.OperatingPowerEase 500A simple operating procedure of the PowerEase 500 Power Supply is providedbelow. For more details on the different screens and custom methods, seepages 14–23.1. Turn on the PowerEase 500 Power Supply.2. Press Select once the Start-Up screen appears.3. Select your gel type from the Gel Method screen.4. Choose the number of gels to be electrophoresed from the Gel Quantityscreen.5. Press the Start button to begin electrophoresis.Note: To reset the PowerEase 500 Power Supply, press the Stop button.NoteThe maximum number of Novex mini-gels that can be electrophoresed at thesame time using PowerEase 500 Power Supply and the XCell SureLock MiniCell is listed in the following table:Gel TypeNumber of GelsNovex Tris-Glycine Gels10 gelsNuPAGE Novex Tris-Acetate Gel6 gelsNuPAGE Novex Bis-Tris Gels2 gelsNovex Tricine Gels4 gelsNovex TBE-Urea gels10 gelsContinued on next page14

Using PowerEase 500, ContinuedStart-Up ScreenThe Start-Up screen is the first screen to appear after pressing the Power buttonon the keypad.Press the Select button to advance to the Gel Method screen.Note: After first use, the last screen previously in use will appear after shuttingthe power off and back on again.Gel MethodScreenThe Gel Method screen (see figure below) allows you to select from a choice ofeight pre-programmed methods for Novex mini-gels or four user-definedmethods.1.Use the directional arrow buttons to navigate through the menu.2.Press the Select button to choose the method. You will advance to the GelQuantity screen (see next page) if a pre-programmed method is chosen, or tothe Custom Method Option screen (see page 22) if a user defined method isselected.Note: The pre-programmed method durations are set to the shortest run time pergel type, or buffer level and buffer strength to prevent over-running a gel.Different percentage gels within a single gel type can cause a gel to take longer torun. Additional time can be added to the pre-programmed method before,during, and after a run (see page 20).Tris-Gly GelTBE GelIEF GelWestern BlotUser-Custom1User-Custom3andTricine GelTBE-Urea GelNuPAGE GelNuPAGE BlotUser-Custom2User-Custom4moves cursorSELECT to continueContinued on next page15

Using PowerEase 500, ContinuedGel QuantityScreenThe Gel Quantity screen (see the following figure) allows you to scale the preprogrammed method’s current and power values by the number of gels that areto be run.1.Use the Up and Down arrows on the keypad to increase or decrease thenumber of gels from 1 to the maximum allowable quantity of gels which canbe run for the specified method.2.Press the Start button to begin the electrophoresis. The Method Run LargeDisplay screen will be displayed.3.If you need to edit any electrophoresis parameters, press the Select button toadvance to the Method Edit screen (see page 19).The Stop button returns you to the Gel Method screen (or the Custom MethodOptions screen if a custom method is in use).If the number of gels selected causes the current to be scaled such that thePowerEase 500 Power Supply maximum power limit of 50 watts is exceeded, anerror message “Power Limit Reached” will be displayed. Similarly, if the currentexceeds 500 mA, an error message “Current Limit Reached” will be displayed.Change the number of gels appropriately to continue electrophoresis.Note: Avoid changing the number of gels during the run, unless gels areremoved during the run. If you would prefer to see the total output parameters,leave the gel number at “1” before starting.Tris-Glycine GelNumber ofGels to be run:2adjust number of gelsSTART begins; SELECT editsContinued on next page16

Using PowerEase 500, ContinuedMethod Run LargeDisplayThe Method Run Large Display screen (see the following figure) is displayedafter the Start button is pressed from the Gel Quantity screen or the MethodEdit screen. This screen displays the status of the method during a run in aneasy to read format. The screen displays: Selected Method Status (running or paused) Number of Gels Run Condition (Limiting Parameters) Actual Voltage and Current (large display) Step Time Remaining Present Step Number Actual Wattage (small display) Detected Temperature (if installed)Pressing the Select button once takes you to the Method Run Graphic Displayscreen (see page 18) and to the Method Run/Edit screen (see page 19) if pressedtwice.The Stop button pauses the run if pressed once, stops the method if the methodis already in the paused state and takes you to the Method Edit screen.To resume, press the Start button.Tris-Glycine GelVoltage is constant125V30mA2 GelsRUNNINGTimeLeft:Step:Power:0:0712WTemp:24 CoSELECT views; STOP pausesContinued on next page17

Using PowerEase 500, ContinuedMethod RunGraphic DisplayThe Method Run Graphic Display screen (see the following figure) isdisplayed after the Select button is pressed from the Method Run LargeDisplay screen. This screen displays the status of the method during a run in agraphical format. The screen displays: Selected Method Status (running or paused) Present Run Time Voltage, Current, and Wattage (applied during the run) Run Condition (Limiting Parameter)The Select button takes you to the Method Run/Edit screen (see page 19).The Stop button pauses the run if pressed once, stops the method if pressedtwice and takes you to the Method Edit screen.To resume, press the Start button.IEF GelRUNNING500V6mAcurrent2:072WvoltspowerSELECT views; STOP pausesContinued on next page18

Using PowerEase 500, ContinuedMethod EditScreenThe Method Edit screen (see the following figure) is displayed after the Selectbutton is pressed from the Gel Quantity screen. The Method Edit screen allowsyou to edit the following method parameters: Step DurationThe maximum step duration is 24 hours. If the duration is set to zero, thestep will be deleted and all following steps will be moved up. If you set anempty step to non-zero, the voltage, current, and wattage settings from theprevious line will be copied. Step Voltage Limit Step Current Limit Step Power Limit Load Check StateThe Load Check state is either ON or OFF, and determines whether or notthe PowerEase 500 Power Supply unit will detect ‘No Load’ conditions. Thedefault value for Load Check is ON and is the recommended state. Gel QuantityThe gel quantity limit determines the maximum voltage, current, andwattage for the step. Values must be in the range of 1–500 volts, 1–500 mA,and 0.1–50 watts for one gel (the values for current and watts will be reducedif multiple gels are selected).If you have changed a pre-programmed method, the method will always run asedited until you cycle back to the Gel Method screen. At this point the preprogrammed method will revert to the default parameters.To edit the parameters on this screen:1.Press the Left or Right arrow buttons to move the cursor between differentfields. As the cursor moves from field to field, a status message on LCDdisplay is updated to show the affected parameters if changes are made.2.Use the Up and Down buttons to adjust field values.3.Press the Start button to begin the run and change the display to the monitorstate. The Stop button takes you to the Gel Quantity screen (see page 16).Tris-Glycine GelStep123Time1:30Load Check: ONo24 CLimits125V 100 mA 18.0Wmoves cursoradjusts run timeSTART begins; STOP for priorContinued on next page19

Using PowerEase 500, ContinuedMethod Run/EditScreenThe Method Run/Edit screen (see the following figure) is displayed after theSelect button is pressed from the Method Run Large Display screen or theMethod Run Graphic Display screen. This screen displays the status of themethod during a run and allows you to edit parameters during the run for thecurrent step displayed on this screen.The screen displays: Selected Method Status (running or paused) Number of Gels Total Time Elapsed Run Condition (limiting parameter or error condition if detected) Detected Temperature (if probe is installed) Present Step Number Present Parameter Set Points Step Time Elapsed Actual Voltage, Current and WattageMake the appropriate change using the directional arrow keys and press theStart button.The Select button takes you to the Method Run Large Display if pressed once,and to the graphic display if pressed twice.The Stop button pauses the run if pressed once and stops the method if themethod is already paused.20

PrintingPrint ScreenPowerEase 500 Power Supply is equipped with a parallel port, allowing you toprint your electrophoresis report.1.If you wish to print a report, you need to connect the PowerEase 500 PowerSupply to a printer. See page 12 for Installing an Optional Printer.2.After a run is completed (time left must be zero), the message “START printsSTOP quits” is displayed at the bottom of the screen.3.Press the Start button to print a report reflecting voltage, current, watts, andtemperature changes which occurred during the run.An example of a report is:Method name: IEF GelRun mVmAWKohms 2321

Custom MethodsCustom MethodOptions ScreenThe Custom Method Options screen (see the following figure) allows you toprogram a custom method. The screen displays the following menu choices: Edit or Run Method Save Method Change Method name Reset Method1. Use the Up and Down arrow buttons to choose from the menu items.2. Press the Select button to select the current menu choice.The Stop button returns you to the Gel Method screen (see page 15).Menu for User-Custom1Edit or Run MethodSave MethodChange Method nameReset Methodmoves cursorSELECT chooses; STOP for priorEdit or RunMethodThe Edit or Run Method option displays the Gel Quantity screen (see thefollowing figure) for the pre-programmed methods. Enter the number of gels tobe run and press Start to begin the run.User-Custom1Number ofGels to be run:1adjust number of gelsSTART begins; SELECT editsIf you need to edit run parameters, press Select to display the Method Editscreen (see figure below).User-Custom1Step123Load Check: ONo24 CTime0:300:301:00Limits25mA100V200V 50mA500V 110mA2.5W9.0W50.0Wmoves cursoradjusts run timeSTART begins; STOP for priorContinued on next page22

Custom Methods, ContinuedSave MethodThe Save Method option saves any changes made to the method during theprevious editing session. It is not necessary to save the method before themethod is run. Method is saved to RAM.Change MethodNameThe Change Method Name screen (see the following figure) allows you tochange a custom method user name. The screen displays the current defaultcustom method user name (i.e. User-Custom1) and a new name field that is usedto change the default method user name.1.Use the Left or Right arrow buttons to change the current character (onlycapital letters, numbers 0 to 9, and a few other characters such as “-” and “\”are available).2.Press the Start button to delete the character directly under the cursor and allremaining characters to the right of the cursor.3.Press the Select button to accept the new name as the name of the methodand return you to the Custom Method Options screen. The Stop buttonabandons changing the method name and returns you to the CustomMethod Options screen.Change Method NameOld Name:New Name:User-Custom1ZYMOGRAMchanges lettermoves cursorSTART erases remaining charsSELECT to accept; STOP exitsReset MethodThe Reset Method screen returns all method parameters including the customname back to the original default parameters.1.To reset, choose “Yes” with the Left button and then press the Selectbutton.2.The custom method is cleared and the Gel Method screen is displayed.Choosing “No” and pressing the Select button, or pressing the Stop buttonreturns you to the Custom Method Options screen.User-Custom1Method will be reset todefault values. Is this OK?YesNOmoves cursorSELECT choice23

TroubleshootingObservationCauseSolutionThe LCD screen remainsblank and the fan does notcome on when the mainpower is turned onPower cord not connected or thefuse has blownCheck power cord connections atboth ends or replace the fuse(see page 29).Secondary power offPress the Power button on thekeypad.Display shows PAUSEand Load ErrorElectrophoresis cell(s) are notconnected, are disconnectedduring the run, or there is abroken connection in theelectrophoresis cellCheck the connections to the powersupply and on your electrophoresiscell to make sure the connection isintact.High resistance due to tape left ona pre-cast gel, incorrect bufferconcentration, or incorrect buffervolumes in the electrophoresis cellPress the Stop button.Correct the condition by makingsure the tape is removed from thepre-cast gel, buffers are preparedcorrectly, and the recommendedvolume of buffer is added to theelectrophoresis cell.Restart the method.Display shows PAUSEand Hardware Error--Reset unit by holding front panelPower button down while switchingrear power switch off and then onagain. Release front Power button.Display shows PAUSE andTemperature Error (iftemperature probe isconnected)Buffer temperature exceeds thepreset temperature limitCheck the run conditions for thecorrect current and power settings.Make sure the buffer is preparedcorrectly.Raise the temperature limit.“Current Limit Reached” isdisplayed on the GelQuantity screenNumber of gels selected exceedsthe set current limitNumber of gels is limited by themaximum current available.Decrease the number of gels orchange the current settings.“Power Limit Reached” isdisplayed on the GelQuantity screenNumber of gels selected exceedsthe set power limitNumber of gels is limited bymaximum power available.Decrease the number of gels orchange the power settings.24

AppendixInstrument Symbols and SafetyAvoidingElectrical ShockThe PowerEase 500 Power Supply produces high voltage outputs which areelectrically isolated from earth ground to reduce the risk of electrical shock tothe user. Observe the following guidelines to ensure safe operation of the unit.The PowerEase 500 Power Supply has been designed for use withelectrophoresis cells with shielded banana plugs thus minimizing potentialshock hazard to the user. We do not recommend using other unshieldedbanana plugs.To avoid electrical shock:Avoiding Damageto the InstrumentSymbols1.Never connect or disconnect wire leads from the power jacks when theamber high voltage indicator light is on.2.Wait at least 5 seconds after stopping a run before handling output leads orconnected apparatus.3.Always make sure hands and work area is clean and dry before making anyconnections or operating the power supply.4.Only connect the power supply to a properly grounded

Plug the power cord of the PowerEase 500 Power Suppl. y into an available electrical outlet rated at the appropriate electrical values for the power supply. 2. Turn on the main power switch located on the rear of the PowerEase 500 Power Supply (see page . 8 for rear view of PowerEase 500 Power Supply). Always leave this switch in the . ON

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