SISTER NIVEDITA UNIVERSITY SYLLABUS FOR THREE

SISTER NIVEDITA UNIVERSITYSYLLABUSFORTHREE YEARS B.Sc. (DEGREE) COURSEINBIOTECHNOLOGYUNDERUGC-CBCS SYSTEM2019

Credit DistributionName of Department: BiotechnologyName of the UG program: B.Sc. (Hons.)Duration of program: 6 Semester (3 years)Head/ In-Charge of the department: Rajat PalSemesterst12nd3rd4th5th6thTotal Credit / CourseTotal EC21211144USC22Total/ Sem2727232318184136CC: Core Courses; GE: General Elective; AECC: Ability Enhancement Compulsory Course; SEC: SkillEnhancement Courses; DSE: Discipline Specific Elective; USC: University specified course

B.Sc. Biotechnology (H) Course StructureCategoryCourse nameCodeCreditTeaching SchemeLTPSemester – ICC – 1CC – 2DSE – 1GE – 1AECC – 1SEC – 1USC – 1Basic concepts of BiologyCell BiologyFundamentals of ChemistryGeneric ElectiveCommunicative EnglishMentored Seminar – IForeign Language I (German/ Spanish/ Japanese)CC – 3CC – 4DSE – 2DSE – 3GE – 2AECC – 2SEC – 2USC – 2Total Credit 27Semester – IIMolecular Biology and Genetic EngineeringBiochemistryBiosafety and BioethicsBiostatisticsGeneric ElectiveEnvironmental ScienceMentored Seminar – IIForeign Language II (German/ Spanish/ Japanese)CC – 5CC – 6DSE – 4GE – 3SEC – 3CC – 7CC – 8DSE – 5GE – 4SEC – 4CC – 9CC – 10CC - 11CC – 12CC – 13Total Credit 27Semester – IIIIntroduction to MicrobiologyBiophysics and InstrumentationEssential PhysicsGeneric ElectiveMentored Seminar – IIITotal Credit 23Semester – IVIntroduction to ImmunologyPrinciples of GeneticsMedical Microbiology and Drug deliveryGeneric ElectiveMentored Seminar – IVTotal Credit 23Semester –VBioprocess Technology and FermentationIntroduction to Bioinformatics, genomics & proteomicsBiodiversity and TaxonomyTotal Credit 18Semester – VIAnimal and plant BiotechnologyProjectTotal Credit 1136664212444321200010004440000Teaching Hour 22/12712366244212442332120001100044000000Teaching Hour ching Hour ching Hour 29114251114252114253666404404404Teaching Hour 241142611143636124040024Teaching Hour 30

CORE COURSES

First SemesterCC 1: Basic concepts of BiologyUnit 1: Organization in Nature;The Scientific Method; Linnaean hierarchical classification system; Evolutionas a Unifying Theme of Life; Origins of Life: Early Chemical and BiologicalEvolution; Oparin-Haldane model; Miller-Urey experiment; Structure of Water;Properties of waterUnit II: Basic units of life;Cellular Foundations; Cells as the Structural and Functional Units of all livingorganisms; Cellular dimensions; Three Distinct Domains of life; Commonstructural features of bacterial cells; Chemical Foundations ; Representationsof molecules; Molecular asymmetry: chiral molecules in life; Conformations;Complementary fit between a macromolecule and a small molecule.Unit III: Physical Foundations;Living Organisms Exist in a dynamic steady state; Macromolecules are majorconstituents of Cell; Three-Dimensional Structure determined byConfiguration and Conformation; Stereospecific interactions betweenbiomolecules; The flow of electrons provides energy for organisms; Energycoupling links reactions in Biology; Enzymes promote sequences of chemicalreactions; Metabolism regulate and achieve balance and economy.Unit IV: Genetic Foundations;Material of heredity; The Structure of DNA allowing for Its replication andrepair with Near-Perfect Fidelity; Mendel and Genetic Continuity; Centraldogma of Molecular Biology; The Linear Sequence in DNA Encodes Proteinswith Three-Dimensional StructuresUnit V: Evolutionary Foundations;Changes in the Hereditary instructions allow evolution; Chemical evolutioncan be simulated in the laboratory; Abiogenesis; The First Cell Was Probablya Chemoheterotroph; Eukaryotic cells evolved from prokaryotes in severalstages; Molecular Anatomy Reveals Evolutionary Relationships; populationgeneticsUnit VI: Macromolecules:Carbohydrates: Structure – Introduction & Occurrence, Classification ofMono-, Di- and Polysaccharides, Reducing & Non-reducing Sugars,Constitution of Glucose & Fructose, Osazone formation, Pyranose & Furanoseforms, Determinationof ring size, Inter-conversion of mono-saccharides

Lipids: Structure– General introduction, Classification & Structure of Simple &Compound lipids, Properties of Lipid aggregates (elementary idea), Biologicalmembrane, Membrane protein – structural aspects, Lipoproteins (elementaryidea).Proteins: Structure– General introduction, Classif ication & Generalcharacteristics, Structure of Primary, Secondary, Tertiary & Quaternaryproteins (elementary ideaText/ Reference Books1. Principles of Biochemistry A. Lehninger2.Molecular Biology of the Cell B.Alberts et alCC1-LAB: Basic concepts of Biology LAB1. Introduction to good lab practice, waste management and safety.2. Preparation of buffers. Concepts of molarity, molality3. pH measurements4. pH Titration of the Glycine HCl solution5. Microscopes and its parts.6. DNA absorption spectra.7. Staining of Plant and Animal Tissues using Single and Double StainingTechniques8. Study of Photomicrographs of Cell Organelles

CC -2 : Cell BiologyUnit 1 : Structure of CellPlasma membrane: Structure and transport of small molecules. Cell Wall:Eukaryotic cell wall, Extracellular matrix and cell matrix interactions, Cell-CellInteractions - adhesion junctions, tight junctions, gap junctions, andplasmodesmata (only structural aspects).Mitochondria, chloroplasts and peroxisomes.Cytoskeleton: Structure andorganization of actin filaments, association of actin filaments with plasmamembrane, cell surface protrusions, intermediate filaments, microtubules.Nuclear envelope, nuclear pore complex and nuclear lamina. Chromatin –Molecular organization. Nucleolus.Unit II: Protein Sorting and TransportEndoplasmic Reticulum – Structure, targeting and insertion of proteins in theER, protein folding, processing and quality control in ER, smooth ER and lipidsynthesis, export of proteins and lipids Golgi Apparatus – Organization,protein glycosylation, protein sorting and export from Golgi Apparatus.Lysosomes.Unit III: Cell SignallingSignalling molecules and their receptors. Function of cell surface receptors.Pathways of intracellular receptors – Cyclic AMP pathway, cyclic GMP andMAP kinase pathway.Unit IV: Cell Cycle, Cell Death and Cell Renewal No. of Hours: 14Eukaryotic cell cycle and its regulation, Mitosis and Meiosis. Development ofcancer, causes, types, Diagnosis and therapy. Programmed cell death. Stemcells. Types: Embryonic stem cell, induced pluripotent stem cells.Text/ Reference Books1. Molecular Biology of the Cell. Bruce Alberts, Alexander Johnson, JulianLewis, David Morgan, Martin Raff, Keith Roberts, and Peter Walter.2. The Cell: A Molecular Approach. Geoffrey M. Cooper, Robert E.Hausman.

3. Essential Cell Biology-Bruce Alberts, Dennis Bray, Karen Hopkin,Alexander D.Johnson, Julian Lewis, Martin Raff, Keith Roberts, and PeterWalter.CC2 LAB: Cell Biology Biology LAB1. Study a representative plant and animal cell by microscopy.2. PTC testing3. .Isolation of chloroplast and Hill reaction.4. Mitochondrial marker enzyme assay.5. Vital staining of mitochondria.6. Differential staining of DNA and RNA.7. Study of different stages of Mitosis by permanent slides.8. Study of different stages of Meiosis by permanent slides9. Mitosis is onion root tip and effect of Colcichine10.Demonstration of dialysis.

Second SemesterCC 3: Molecular Biology and Genetic EngineeringCOURSE PERPOSEpurpose of this course is to introduce students to basic molecular biologicalconcepts and techniques used in the fields of biotechnology and geneticengineering.Unit I : Introduction to Molecular BiologyDNA AND RNA Scope and History. Structure of DNA-Nucleoside, Nucleotide,Base pairing, Base stacking, Double Helix, features of Watson and Crickmodel, major and minor groove, Supercoiling- twist, writhe and linkingnumber. Forms of DNA- A, B, Z. Structure and function of mRNA, rRNA, tRNA.Secondary structures in RNA.Unit II:Replicaiton and Repair:Types and functions of DNA polymerases in Prokaryote and Eukaryote.Replication in prokaryote and Eukaryote. Proof reading activity, 5’- 3’exonuclease activity, topoisomerase activity, Telomeric DNA replication andPlasmid Replication-theta model, strand displacement model and rollingcircle model. DNA Repair- Nucleotide excision repair, base excision repair,mismatch repair, photo-reactivation, recombination repair and SOS repair,mechanisms of DNA recombination; mutagenesis and processes of DNArepairUnit III: Transcription and Post Transcriptional Modifications)Fine structure of prokaryotic and eukaryotic gene, structure and function ofthe promoters in mRNA, rRNA, tRNA genes. RNA polymerases in prokaryoteand eukaryote, types and function. Transcription of mRNA, rRNA, and tRNAgenes in Prokaryote and eukaryote. Post transcriptional processing of mRNA

– 5’capping, splicing (including different types), polyadenylation and RNAediting.Unit IV: Translation and Post Translational ProcessingGenetic code and Wobble hypothesis. Translation in prokaryote andeukaryote. Post translational modifications. Principles protein sorting andtargeting into endoplasmic reticulum, mitochondria, chloroplast, and nucleus.Various forms of Bacterial immunity ( RM, CRISPR etc.)Site-directedmutagenesis and its applications; transposons, site specific recombination;polymerase chain reaction (PCR); applications of reverse transcription PCR(RT-PCR) and real time PCR; principles and applications of DNA fingerprinting; gene mapping by restriction fragment length polymorphism (RFLP);application of differential display and subtractive hybridization., genetargeting and CRISPR-CASUnit V: Gene RegulaitonPrinciples of gene regulation- Transcriptional and post transcriptional s,co-suppressors,moderators, silencers, insulators, enhancers. Operon-lac operon, trp operon,ara operon and gal operon. regulation of gene expression; Gene Silencing:PTGS & TGS; eukaryotic RNA splicing and processingUnit VI: Genetic engineeringRestriction modification enzymes; cloning vectors: plasmids, phages,cosmids, phagemids, yeast and bacterial artificial chromosomal vectors;construction cDNA and genomic libraries; screening of libraries: by DNAhybridization, immuno and protein assays; gene cloning and expression inprokaryotes and eukaryotes; recombinant protein expression in E. coli, yeastand baculovirus; mammalian cell expression vectors (Selectable markers,Two-hybrid expression system); chimeric vectors;Texts/ References1. 1.B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter,Molecular Biology of Cell, 4th Ed., Garland Publishing, 2002.2. 2.H. Lodish, A. Berk, S. L. Zipursky, M. P. Scott and J. Darnell,Molecular Cell Biology, 4th Ed., W. H. Freeman & Co., 2003.3. B. Lewin, Genes VIII, International Edition, Pearson Education, 2004.4. B. R. Glick and J. J. Pasternak, Molecular Biotechnology: Principlesand Applications of Recombinant DNA, 3rd Ed., ASM Press, 2003.