EZ1 Advanced XL User Manual - Frank's Hospital Workshop

Safety Information1.5ChemicalsWARNINGHazardous chemicals[W8]Some chemicals used with this instrument may behazardous or may become hazardous after completionof the protocol run.Always wear safety glasses, gloves, and a lab coat.The responsible body (e.g., laboratory manager) musttake the necessary precautions to ensure that thesurrounding workplace is safe and that the instrumentoperators are not exposed to hazardous levels of toxicsubstances (chemical or biological) as defined in theapplicable Material Safety Data Sheets (MSDSs) orOSHA, ACGIH, or COSHH documents.Venting for fumes and disposal of wastes must be inaccordance with all national, state, and local health andsafety regulations and laws.OSHA:Occupational Safety and Health Administration (United States of America).ACGIH:American Conference of Government Industrial Hygienists (United States of America).COSHH: Control of Substances Hazardous to Health (United Kingdom).Toxic fumesIf you work with volatile solvents, toxic substances, etc., youmust provide an efficient laboratory ventilation system toremove vapors that may be produced.1.6Waste disposalUsed labware, such as reagent cartridges and filter-tips, maycontain hazardous chemicals or infectious agents from thepurification process. Such wastes must be collected anddisposed of properly in accordance with the local safetyregulations.For disposal of waste electrical and electronic equipment(WEEE), see page A-6.1-6EZ1 Advanced XL User Manual 05/2009

Safety Information1.7Mechanical hazardsThe worktable of the EZ1 Advanced XL moves duringoperation of the instrument.WARNINGMoving partsNever open the EZ1 Advanced XL door while the EZ1Advanced XL is operating.WARNINGRisk of overheating[W10]To ensure proper ventilation, maintain a minimumclearance of 15 cm and 30 cm at the rear and sides ofthe EZ1 Advanced XL, respectively.Slits and openings that ensure the ventilation of the EZ1Advanced XL must not be covered.1.8[W9]Heat hazardThe EZ1 Advanced XL worktable contains a heating system.WARNING1.9WARNINGHot surface[W11]The heating system can reach high temperatures. Avoidtouching it when it is hot.UV radiationThe EZ1 Advanced XL contains 2 UV lamps[W12]Avoid looking directly into UV light. Do not expose yourskin to UV light.The wavelength of the UV light produced by the UV lamp is253.7 nm. This wavelength corresponds to ultraviolet lighttype C, which can be used for decontamination procedures.EZ1 Advanced XL User Manual 05/20091-7

Safety InformationWARNING1.10Symbol1-8UV lamp[W13]The UV lamp consists of a robust glass cylinder. It issafely located inside the door of the EZ1 Advanced XL. Incase of accidental breakage of the lamp, contactQIAGEN Technical Services or your local distributor toarrange proper disposal of the lamp.Symbols on the EZ1 Advanced XLLocationDescriptionHeatingsystemHeat hazard — the temperature of theheating system can reach up to 95ºCNear thetip rackBiohazard — the tip rack may becontaminated with biohazardous materialand must be handled with glovesOn theback of theinstrumentUV radiation hazard. UV light could burnyour skin and cause damage to your eyeNear thepipettorheadCrush hazard — the nozzle unit may crushyour fingers or handNear thetip rackUV lamp heat hazard — the UV lamp is hot.Do not touch the UV lampEZ1 Advanced XL User Manual 05/2009

Safety pe plateon theback of theinstrumentCE mark for EuropeType plateon theback of theinstrumentUL listing mark for Canada and the USAType plateon theback of theinstrumentRoHS mark for China (the restriction of theuse of certain hazardous substances inelectrical and electronic equipment)Type plateon theback of theinstrumentWEEE mark for EuropeType plateon theback of theinstrumentSerial numberType plateon theback of theinstrumentLegal manufacturerAdditional symbols in this user manualLocationFrontcoverDescriptionCatalog numberEZ1 Advanced XL User Manual 05/20091-9

Safety bookSymbol on the heating system.Symbol near the tip rack.1-10EZ1 Advanced XL User Manual 05/2009

Safety InformationSymbol on back of the EZ1 Advanced XL.EZ1 Advanced XL User Manual 05/20091-11

Safety InformationThis page intentionally left blank1-12EZ1 Advanced XL User Manual 05/2009

Introduction2IntroductionThank you for choosing the QIAGEN EZ1 Advanced XL. Weare confident it will become an integral part of yourlaboratory.Before using the instrument, it is essential to read thismanual carefully and to pay particular attention to anyadvice it contains concerning hazards that may arise fromuse of this instrument.2.1About this user manualThis manual provides information about the EZ1 AdvancedXL. It guides you systematically through the followingsections:1. Safety Information2. Introduction3. General Description4. Installation Procedures5. General Operation6. Preventive Maintenance7. Troubleshooting8. GlossaryAppendicesThe Appendices contain the following: Technical data Liability clause2.2General information2.2.1Technical assistanceAt QIAGEN we pride ourselves on the quality and availabilityof our technical support. Our Technical Service Departmentsare staffed by experienced scientists with extensive practicaland theoretical expertise in molecular biology and the use ofQIAGEN products. If you have any questions regarding theEZ1 Advanced XL User Manual 05/20092-1

IntroductionEZ1 Advanced XL or QIAGEN products in general, please donot hesitate to contact us.QIAGEN customers are a major source of informationregarding advanced or specialized uses of our products. Thisinformation is helpful to other scientists as well as to theresearchers at QIAGEN. We therefore encourage you tocontact us if you have any suggestions about productperformance or new applications and techniques.For technical assistance and more information, please seeour Technical Support Center at www.qiagen.com/Supportor call one of the QIAGEN Technical Service Departments orlocal distributors (see back cover or visit www.qiagen.com).2.2.2Policy statementIt is the policy of QIAGEN to improve products as newtechniques and components become available. QIAGENreserves the right to change specifications at any time.In an effort to produce useful and appropriatedocumentation, we appreciate your comments on thispublication. Please contact QIAGEN Technical Services.2.2.3Version managementThis document is the EZ1 Advanced XL User Manual, revisionR1.2.3Intended use of the EZ1 Advanced XLThe EZ1 Advanced XL is designed to perform automatedisolation and purification of nucleic acids.It is intended to be used only in combination with QIAGENkits indicated for use with the EZ1 Advanced XL instrumentfor the applications described in the kit handbooks.The system is intended for use by professional users, such astechnicians and physicians trained in molecular biologicaltechniques and the operation of the EZ1 Advanced XL.2-2EZ1 Advanced XL User Manual 05/2009

Introduction2.3.1Requirements for EZ1 Advanced XL usersThis table covers the general level of competence andtraining necessary for transportation, installation, use,maintenance, and servicing of the EZ1 Advanced XL.Type of taskPersonnelTraining and experienceTransportationNo specialrequirementsNo special requirementsInstallationLaboratorytechnicians orequivalentAppropriately trained andexperienced personnelfamiliar with use of computersand automation in generalRoutine use(runningprotocols)Laboratorytechnicians orequivalentAppropriately trained andexperienced personnelfamiliar with use of computersand automation in generalRegular, daily,and weeklymaintenanceLaboratorytechnicians orequivalentAppropriately trained andexperienced personnelfamiliar with use of computersand automation in generalServicing andannual lists onlyEZ1 Advanced XL User Manual 05/20092-3

IntroductionThis page intentionally left blank2-4EZ1 Advanced XL User Manual 05/2009

General Description3General DescriptionThe EZ1 Advanced XL performs fully automated nucleic acidpurification from up to 14 samples using magnetic particles.The automated steps include: Reading reagent and sample information with ahandheld bar code scanner connected to the EZ1Advanced XL Lysis of samples Binding of nucleic acids to magnetic particles Washing and elution of nucleic acids Generating a report file that either will be transmitted toa PC or printed on an external printer after the protocolrun is finished Using UV radiation for decontaminationThe user inserts an EZ1 Advanced XL Card containingprotocol(s) into the EZ1 Advanced XL. After starting worktablesetup using the control panel and bar code reader, the userloads samples, reagent cartridges, filter-tips in tip holders,and elution tubes onto the EZ1 Advanced XL worktable.The user then closes the EZ1 Advanced XL door and startsthe protocol. The door locks automatically at the start of theprotocol. The protocol provides the necessary instructions forthe EZ1 Advanced XL to carry out automated nucleic acidpurification.The aspiration and dispensing of samples and reagents andthe separation of magnetic particles are performed by the14-channel pipettor head. The temperature of samples isregulated by a heating system.EZ1 Advanced XL User Manual 05/20093-1

General Description3.1External features of the EZ1 Advanced XL4231Front view of the EZ1 Advanced XL.3.1.11EZ1 Advanced XL door3EZ1 Advanced XL Card slot2Control panel4Status LEDsEZ1 Advanced XL doorThe EZ1 Advanced XL door can be manually opened by theuser in order to gain access to the worktable when noprotocol is running. A protocol cannot be started until theEZ1 Advanced XL door is closed.3.1.2Control panelThe control panel is the user interface that allows the user tooperate the EZ1 Advanced XL. It consists of a keypad and avacuum fluorescent display (VFD).3-2EZ1 Advanced XL User Manual 05/2009

General DescriptionKeypadThe following keys of the control panel are available to theuser.KeyDescription0–9Selects a menu or specifies processparameters (e.g., sample volume).STARTInitiates an action or starts a protocol.STOPInterrupts a protocol run.ESCDisplays the previous menu or text.Up/DnUp and down arrow key used, forexample, in the system setup menu.ENTEnter key used, for example, in the systemsetup menu to acknowledge data entry.BSBackspace key used to remove incorrectlyentered values during data entry.SHIFT Up/DnUsed to enter the installation date.The other keys of the control panel are for service personnelonly.Vacuum fluorescent display (VFD)The display consists of 4 lines with 20 characters per line.EZ1 Advanced XL User Manual 05/20093-3

General DescriptionVacuum fluorescent display (VFD).3.1.3EZ1 Advanced XL Card slotThe EZ1 Advanced XL Card slot accepts the EZ1 Advanced XLCard. Each EZ1 Advanced XL Card contains one or moreprotocols that allow the EZ1 Advanced XL to perform nucleicacid isolation.CAUTIONDamage to the instrument[C3]Only use QIAGEN EZ1 Advanced XL Cards with the EZ1Advanced XL. Other EZ1 Cards cannot be used with theEZ1 Advanced XL.Note: Do not insert the EZ1 Advanced XL Card while the EZ1Advanced XL is switched on. EZ1 Advanced XL Cards mustnot be exchanged while the EZ1 Advanced XL is switched on.Take care not to expose the EZ1 Advanced XL Card toelectric shock, water, or dirt.3-4EZ1 Advanced XL User Manual 05/2009

General DescriptionEZ1 Advanced XL Card inserted into EZ1 Advanced XL Card slot.3.1.4Status LEDsThere are 2 light-emitting diodes (LEDs) at the front of theEZ1 Advanced XL: Green LED — indicates that the instrument is receivingpower Red LED — indicates that an error has occurred3.1.5Connector panelThe connector panel is located at the rear of the EZ1Advanced XL.EZ1 Advanced XL User Manual 05/20093-5

General DescriptionConnector panel.It contains the power switch, the socket for the power cord,the fuse box, and two RS232 connectors. The left connectoris used to connect the bar code reader. The right connector isused to connect the EZ1 Advanced XL to a PC or an RS232printer (optional).3.2Internal features of the EZ1 Advanced XLThe interior of the EZ1 Advanced XL contains the worktableand the pipettor head.3.2.1WorktableThe worktable contains various racks for holding thelabware, samples, and reagent cartridges that are requiredfor the protocol run. The worktable also contains a heatingsystem for controlling the temperature of samples.3-6EZ1 Advanced XL User Manual 05/2009

General DescriptionPipettor headHeating systemReagent cartridges incartridge rackSample tubesTip holders with tipsin tip rackElution tubesThe EZ1 Advanced XL worktable.Tip rackThis rack is located at the front of the worktable. The frontrow holds up to fourteen 1.5 ml elution tubes. Therecommended elution tubes are screw-capped, made ofpolypropylene, supplied by Sarstedt (cat. no. 72.692), andprovided in EZ1 Kits.The next 2 rows hold up to 28 tip holders containing filtertips. (Note: Some protocols require only one row of filtertips.) The remaining row holds up to 14 sample tubes.Sample tubes are 2 ml in volume, screw-capped, made ofpolypropylene, supplied by Sarstedt (cat. no. 72.693), andprovided in EZ1 Kits.EZ1 Advanced XL User Manual 05/20093-7

General DescriptionTip rack.Both tip holders and filter-tips are made of polypropyleneand are provided in EZ1 Kits. The filter-tips have a capacityof 50–1000 μl.3-8EZ1 Advanced XL User Manual 05/2009

General DescriptionTip holders and filter-tips.Cartridge rackThis rack is located behind the tip rack and holds up to14 reagent cartridges. Insert the cartridge rack with thearrow pointing towards the instrument.Cartridge rack.Reagent cartridges are made of polypropylene, containprefilled reagents, and are available from QIAGEN.EZ1 Advanced XL User Manual 05/20093-9

General DescriptionHeatingpositionsReagent wellsReagent cartridges.A reagent cartridge consists of 10 reagent wells and2 heating positions. One heating position is a well, the otheris a slot that can accept a tube.Heating systemThe heating system is located under the far end of thecartridge rack. It holds the heating positions of the reagentcartridges.TrayThe tray is located under the racks and collects any drops ofliquid that may fall.Tray.3-10EZ1 Advanced XL User Manual 05/2009

General Description3.2.2Pipettor headThe pipettor head is mounted above the worktable andmoves in the Z direction (i.e., up and down) in order to reachthe samples and reagents on the worktable. The worktableitself moves in the Y direction (i.e., front to back) in order topresent different samples and reagents under the pipettorhead.The pipettor head contains 14 high-precision syringe pumpsthat are connected to tip adapters that can be attached tofilter-tips. The syringe pumps operate simultaneously to allowaspiration or dispensing of small volumes of liquid (50–1000 μl) via the filter-tips.The pipettor head also contains a magnet whose distancefrom the attached filter-tips can be varied. This feature allowsthe capture of magnetic particles that are present in theliquid aspirated into the filter-tips.Located behind the tip adapters is the piercing unit, a row of14 metal spikes for puncturing the foil that covers thereagent cartridges.During operation, the piercing unit, controlled by the EZ1Advanced XL, opens the reagent cartridges. The pipettorhead then automatically picks up filter-tips from the tip rackand performs aspiration and dispensing operations atdifferent locations on the worktable before ejecting the tipsback into the tip rack.EZ1 Advanced XL User Manual 05/20093-11

General DescriptionPipettor head.3.2.3UV lampThe EZ1 Advanced XL is equipped with 2 UV lamps, locatedunder the blue EZ1 Advanced XL door. The UV lamps can bemanually switched on and off in a separate menu, asdescribed later in this manual.Do not touch the UV lamp with your fingers since it mayreduce the lamp’s lifetime. The EZ1 Advanced XL door will belocked during a UV light cycle in order to protect theoperator from UV radiation. The EZ1 Advanced XL willprovide a warning when the lifetime of the lamp is going toexpire. Call QIAGEN Technical Services when a UV lampneeds to be replaced.WARNING3-12The EZ1 Advanced XL contains 2 UV lamps[W12]Avoid looking directly into UV light. Do not expose yourskin to UV light.EZ1 Advanced XL User Manual 05/2009

General DescriptionCAUTIONLifetime UV lamp[C4]The UV lamps need a minimum switch-on time of20 minutes. Do not interrupt a UV light cycle before20 minutes have passed since it will reduce the lamp’slifetime.UV lamps and reflector case.UV lampsLocation of UV lamps under the EZ1 Advanced XL door.EZ1 Advanced XL User Manual 05/20093-13

General DescriptionCAUTION3-14Cover of UV lampsBe careful not to damage the cover of the UV lampswhen loading and unloading the worktable.[C5]EZ1 Advanced XL User Manual 05/2009

Installation Procedures4Installation ProceduresThe EZ1 Advanced XL is a plug-and-play instrument, makingunpacking and installation easy. A member of your groupwho is familiar with laboratory equipment should oversee theinstallation.4.1RequirementsSiteThe EZ1 Advanced XL must be located out of direct sunlight,away from heat sources, and away from sources of vibrationand electrical interference. Refer to Appendix A for theoperating conditions (temperature and humidity).Use a level workbench that is large and strong enough toaccommodate the EZ1 Advanced XL. Refer to Appendix A forthe weight and dimensions of the EZ1 Advanced XL.The EZ1 Advanced XL must be placed near to a properlygrounded (earthed) AC power outlet. The power line to theinstrument should be voltage regulated and surge protected.4.2AC power connectionPower requirementsThe EZ1 Advanced XL operates at: 100–120 V AC 10%, 50/60 Hz, 600 VA 200–240 V AC 10%, 50/60 Hz, 600 VAMake sure the voltage rating of the EZ1 Advanced XL iscompatible with the AC voltage available at the installationsite.Grounding requirementsTo protect operating personnel, the EZ1 Advanced XL mustbe correctly grounded (earthed). The EZ1 Advanced XL isequipped with a 3-conductor AC power cord that, whenconnected to an appropriate AC power outlet, groundsEZ1 Advanced XL User Manual 05/20094-1

Installation Procedures(earths) the EZ1 Advanced XL. To preserve this protectionfeature, do not operate the EZ1 Advanced XL from an ACpower outlet that has no ground (earth) connection.Installation of AC power cordConnect one end of the AC power cord to the socket locatedon the rear of the EZ1 Advanced XL and the other end to theAC power outlet.The fuse box of the EZ1 Advanced XL is located under thesocket for the power cord and contains 2 fuses, which arelabeled as follows: 110–120 V — a 6.3 A (250 V) time-delay fusecompatible with 100–120 V power supplies 220–240 V — a 3.15 A (250 V) time-delay fusecompatible with 200–240 V power suppliesBefore connecting the instrument to the power supply, y

4.4.3 Installing the EZ1 Advanced Communicator software 4-16 4.5 Installing multiple EZ1 Advanced XL instruments 4-16 4.6 Transportation and removal of the EZ1 Advanced XL 4-20 5 General Operation 5-1 5.1 Overview 5-1 5.2 Inserting and removing the EZ1 Advanced XL Card 5-2 5.2.1 Inserting the EZ1 Advanced XL Card 5-2