SUCCESSFUL USE OF A HIGH- PROTEIN CO-PRODUCT (Still

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SUCCESSFUL USE OF A HIGHPROTEIN CO-PRODUCT (Still Pro50TM) IN TURKEY POULT DIETSDr. Pete Williams – Chief Nutritionist23rd Annual Distillers Grains Symposium15 May 2019 10.15-11.30

Who We AreFluid Quip Process Technologies, LLC (FQPT) provides fully integrated custom technologies and servicesto dry-grind ethanol and biochemical facilities which include full plant design, process optimization studies,yield improvement technologies, new co-product technologies, and turnkey capital projects.EngineeringSolutions ProcessOptimization1.9 billion gallons per year of biofuels production usingFQPT technologiesActive projects and client support in the USA, Canada,South America and Europe70 industry leading pinch-point andde-bottlenecking projectsFull ologiesA teamof dedicatedengineers todrive valuefor your plant.

PROTEIN?All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Global protein demand for feedAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Is it correct to refer to the Dry Grind Ethanol plant?An established global feed and energy industry27 bill gallons ethanol60 mill metric tons DDGS20 mill metric tons PROTEINWorld soy meal 234 millmetric tonsDDGS global volume greater than rapeseed plus fishmealAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

US feed industry has high demand for quality proteinUSA 23.6 mill tonnesof compound feed13 mill tonnes demandsspecialist protein (55%)4 mill tonnes high conc andbenign proteinAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Alternative sources of protein to replace animal proteinFocus on plant derived proteinsBy 2025 fishmeal and other animal protein sources an exception in livestock dietsAnimal derived proteinsMarket ConcernsPlant proteins Highly digestible Sustainability issues Ideal amino acid Political and public concern over safety composition Variation in composition Biogenic amines products History of useDioxin content Bacterial contamination (Ecoli/Salmonella) Viral issues (African Swine Fever) Safer alternativeInvariably cheaperLess digestibleAnti Nutritional FactorsProducts with improveddigestibilityLong term trend to replace animal derived proteins with plant proteinAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Alternative sources of protein to increase supplyInsects; Algae; Single cell proteins; Designer Crops (plant proteins) (Simm 2017)What constitutes commercially viable?NutritionalMarketRegulatory Compliant (safe and Resilience in the supply chainefficacious/ absence of ANF’s) Critical volume ( 100K tons per annum) Highly consistent quality Established logistics Least cost formulation cost competitive Realistic return on investment Well suited to market demandsConsumer acceptable All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Soybean meal is not the ideal alternative protein supplement0% SBM 0%SBM22%SBM47%SBM70%SBMSBM has a dose dependent impact on gut microbiotaHigh levels of SBM reduce lactic acid bacteria and increaselevels of opportunistic pathogensHigh levels of SBM have negative impact on histologyPossible contributory agent to enteritisShuyan Miao 2018 10% SBM35% SBMSBM has a significant impact on gut morphology 10% SBM induced severe morphological changesIncreased SBM levels reduced digestive enzyme capacityPossible causative agent of enteritisKrogdahl et al 2003All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Fore and Against DDGSForeVolumeLogisticsRegulationSafetyDiscounted priceEstablished feed adoptionLocationAgainstNutritional CompositionInconsistent qualityMedian valueBy-product reputationDiscounted priceImperfect feedLimited feed applicationEnergetically expensive productionStrong incentive to do betterAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

MSCTM processAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Commercial alternative protein SP 50Maximised Sillage CoproductsA high (50%) protein productderived from bioethanol stillage Corn gluten proteins plus post fermentation yeast (25%) Growth-promoting and immunomodulatory properties Applications in neonate and aquaculture nutrition Commercial tons growing (300K tons in 2019) High consistency within and between plants(SP 50: Sill Pro 50TM)Alternative High Value ProteinProximate Composition (as is)%Dry Mater (min)Crude Protein%Fat%Fiber%NDF%Starch%Sugar%Ash%TMEn (poultry)kcal/kgAME (poultry)kcal/kgIleal dig aa/Total aaAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights 450.90

Fermentation generates yeast in producing ethanol0.7M gal1000 tonsgrain100kgYeast 39 tons yeastperfermentation⅓ EtOH⅓ DDGS⅓ CO2275 tons DDGS:31% protein85 tons proteinDDGSDDGSDDGSDDGSYeast20% of DDGS protein is yeast protein* 16.5 t yeast protein (39 tons yeast)An operational bioethanol plant produces 8k tons spent yeast / annum*Han and Liu 2010All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

SP 50TM contains yeast cell wall and cell content materialYeast– Appetite stimulant – high glutamic acid content–As a probiotic – modulation of gut microbiota, improved innate immunity, improveddisease resistance, improved growth performanceYeast β-glucan– Improved innate immunity, improved disease resistance, improved growth performance.Yeast MOS– Modulation of gut microbiota, improved innate immunity, improved disease resistance,improved growth performance, improved gut morphology and immunity.Yeast nucleotides– Modulation of gut microbiota, improved innate immunity, improved disease resistance,improved growth, improved gut morphology and immunity, improved mucus productionVitamins & Minerals– B group vitamins; yeast biocomplexed mineralsAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.15

value of residual DDGS is keyProximate composition of DDGS pre and post MSCTM installation28.527.812.17.47.17.6As receivedPre SP50 STDvPre SP50 1.33Improved consistency of DDGS product (30%)All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Pre and Post MSCTM the nutrient composition of DDGSremains very similarAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Post MSCTM gentle drying improves protein valueAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Feeding trial summaryAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Summary FQPT feeding trials with Still Pro 50TMIn total 29 trials completed with partner organizationsComprehensive portfolio of trials demonstrates efficacyTrial TitleAnalytical dataIleal digestibility of SP 50 and standard DDGSEffect of SP 50 on broiler performanceEffect of SP 50 on the performance of broilersSwine digestibility of SP 50Fishmeal replacement with SP 50 for young swineReplacement of SPC with Still Pro50 in trout dietsStill Pro50 as a protein supplement for shrimp and TilapiaEvaluation of SP 50 as a ruminal proteinComparison of SP 50 with commercial RPAA in gas testMeasurement of yeast content in SP 50Available lysine in SP 50 (Carpenter Lysine)SP 50 Protein supplement for turkey poultsSP50 Protein supplement for Atlantic salmonImpact of MSC on DDGS qualityCentre and trials leaderProf P. WilliamsUniv of Ilinois: Prof C. ParsonsColorado Quality Research CQRColorado Quality Research CQRUniv of Ilinois: Prof H. SteinUniv of Ilinois: Prof H. SteinUniversity of Idaho: Prof R. HardyUniv of Auburn: Prof A DavisCumberland labsUniv of Nottingham: Dr J. MargerisonEnglyst carhohydrates: Dr K. EnglystSciantec: Prof P. WilliamsUniv Nottingham Trent: Dr E. BurtonCATC New Brunswick CanadaFQT dataResults49% protein89% ileal protein digestibiility5% inclusion of SP505% inclusion of SP50Standardised ID of aa 77.1%Partial replacement of FMReplacement of soya isolate15% inclusion of SP5074% RUPEquivalent to market leader24% yeast in DMAverage available lysine 98%Improved N & R retentionPendingImproved DDGS qualityAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Replacement of soybean meal with Still Pro 50TMDiet formulation for turkey poultsPeriodControlStill Pro 50Soy isolateHIPROSOYAMEALTG MAIZEHI-PROSUNFLOWERPROTEINME MJ/kg0.00.0Starter 0-21SP50 4% SP50 635.53.03.027.611.928.211.9Control0.00.0Grower 21-42SP50 4% SP50 912.225.512.226.112.224.912.2Dietary inclusion of SP50TM reduced the soybean inclusion by 11%Replaced a soy protein isolate in Premium dietUniversity of Nottingham Trent UKAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Application of Still Pro 50TM in neonatesReplacement of 15% soy protein with SP 50TM resulted in 6%increase in growth of turkey poultsBW d42 0 dietsDay 42 AMEn MJ/kg dietDay 42 N retention g/kg diet0baab14aab4% SP508% SP50Premiumb12108642Control4% SP508% SP50Premium0ControlImprovements in nitrogen and energy retentionAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Application of Still Pro 50TM in aquacultureSP50TM replacement of 16% soy protein concentrate in juvenile trout diets0400Mean weight gain (%)80012001600Survival (%)Feed consumed (g/fish)Weight gain (g/fish)Tendency for SP50TM to outperform controlsCountering soy-induced enteritis?Final weight (g/fish)Condition factorProtein efficiency ratio (PER)Feed conversion ratioDaily feed consumption (% Daily growth index (DGI, g1/3/d)Specific growth rate (SGR, %/d)R.Hardy: Aquaculture Research InstituteU of IdahoSP50TMControl01234All contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.5

Conclusions: A commercial protein supplement forturkey poults from dry grind corn ethanol processing SP 50TM high density protein meal separated from whole stillage A suitable protein supplement in starter diets (turkey poult, aqua etc.) BW gain and N retention improved by replacing 15% of soybean dietary N with SP 50TM Partial replacement of soybean meal; Reduced exposure to soya antigenic factors Reduced intestinal inflammation A positive change in gut architecture Changed gut microbiota to a more positive microbiome Significant growth and health benefits from SP 50TM inclusionAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

A paradigm shift in nutrition strategy for feed and foodFeeding the high performing Olympic athleteWhole grains based feedTraditional feed formulationMinimal raw material complexityStrong regional identityDesigner FeedsFunctional ingredient based feedsTarget functional characteristicsComplex raw material matrixNo regional identityIncrease in production cost requires economies of scaleMore complex diets are more profitableSimple grain processing toaccommodate mixingPelleting to eliminate selection20 – 25% proteinHigh /enzymes/pre-digestion/coatingNutrient dense: 40-80% proteinDEMAND high quality nutrients SP 50TMAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.

Protein: Still Pro 50TMA Solution at HandAll contents are to be considered confidential and proprietary work product of FQPT. 2018 Fluid Quip Process Technologies, LLC. All rights reserved.pwilliams@fluidquiptechnologies.com

May 07, 2019 · SUCCESSFUL USE OF A HIGH-PROTEIN CO-PRODUCT (Still Pro 50TM) IN TURKEY POULT DIETS Dr. Pete Williams – Chief Nutritionist 23 rd Ann

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