AP STATE COUNCIL OF HIGHER EDUCATION ZOOLOGY COURSE .

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AP STATE COUNCIL OF HIGHER EDUCATIONZOOLOGY COURSE STRUCTUREUNDERCBCS (w.e.f. 2015-16, Revised)YEARSEMESTERPAPERTITLEMARKSCREDITSBiology of Non-chordates10003IIPractical - I5002IBiologyofChordates10003IIIIPractical - ractical - III5002IIEmbryology, Physiology and10003IVIVEcologyPractical - IV5002AnimalBiotechnology10003VPractical - V5002VAnimal Husbandry10003VIPractical - VI500210003*Any oneVII (A)* ImmunologyPracticalVII(A)5002Paper from10003VII (B)* Cellular Metabolism andA, B and CMolecular BiologyPractical - VII (B)5002** Any oneBioinformatics10003VII (C)*clusterPractical - VII (C)5002from I, IICluster Electives –VIII-A :ClusterMedical Diagnosticsand III1. Clinical Biochemistry10003VIII-A**2. Haematology100033. Clinical Microbiology10003Practical – VIII: 15002Practical – VIII: 25002Project culture10003VIII-B** 1. Principles of Aquaculture2. Aquaculture Management100033. Postharvest Technology10003Practical – VIII: 15002Practical – VIII: 25002Project Work5002Cluster Electives – VIII-C :SericultureCluster10003VIII-C** 1. Gen. Sericulture,Mulberry cultivation andManagement2. Biology of Mulberry10003Silkworm and Silkwormrearing Technology100033. Silk Technology, SilkMarketing and ExtensionPractical – VIII: 15002Practical – VIII: 25002Project Work5002

AP STATE COUNCIL OF HIGHER EDUCATIONw.e.f. 2015-16 (Revised in April, 2016)ZOOLOGY SYLLABUS FOR I SEMESTERZOOLOGY - PAPER - IANIMAL DIVERSITY - NONCHORDATESPeriods:601.11.21.32.12.2Max. Marks:100Brief history, Significance of Diversity of Non ChordatesProtozoa1.2.1 General characters1.2.2 Classification of Protozoa up to classes with examples1.2.3 Elphidium (type study)Porifera1.3.1 General characters1.3.2 Classification of Porifera up to classes with examples1.3.3 Sycon – External Characters, Types of cells,1.3.4 Skelton in Sponges1.3.5 Canal system in spongesUnit - IICoelenterata2.1.1 General characters2.1.2 Classification of Coelenterata up to classes with examples2.1.3 Obelia - External Characters, Structure of Polyp and Medusa2.1.4 Polymorphism in coelenterates2.1.5 Corals and coral reef formationPlatyhelminthes2.1.1 General characters2.1.2 Classification of Platyhelminthes upto classes with examples2.1.3 Fasciola hepatica - External Characters, Excretory system, Reproductive System,Life History and pathogenicityUnit - III3.13.2Nemathelminthes3.1.1 General characters3.1.2 Classification of Nemathelminthes up to classes with examplesAnnelida3.2.1 General characters3.2.2 Classification of Annelida up to classes with examples3.2.3 Hirudinaria granulosa - External Characters, Digestive System, Excretory Systemand Reproductive System3.2.4 Coelomoducts3.2.5 Vermiculture - Scope, significance, earthworm species, processing, Vermicompost,economic importance of vermicompost

Unit - IV4.14.25.15.25.3Arthropoda4.1.1 General characters4.1.2 Classification of Arthropoda up to classes with examples4.1.3 Prawn - External Characters, Appendages, Respiratory system and CirculatorySystem4.1.4 Peripatus - Structure and affinitiesMollusca4.2.1 General characters4.2.2 Classification of Mollusca up to classes with examples4.2.3 Pearl formation in Pelecypoda4.2.4 Torsion in gastropodsUnit - VEchinodermata5.1.1 General characters5.1.2 Classification of Echinodermata up to classes with examples5.1.3 Water vascular system in star fishHemichordata5.2.1 General characters5.2.2 Classification of Hemichordata up to classes with examples5.2.3 Balanoglossus - Structure and affinitiesNon-Chordata larval forms5.3.1 Amphiblastula5.3.2 Ephyra5.3.3 Trochophore5.3.4 Nauplius5.3.5 Glochidium5.3.6 Bipinnaria5.3.7 Tornaria

ZOOLOGY MODEL PAPER FOR I SEMESTERZOOLOGY - PAPER - IANIMAL DIVERSITY - NONCHORDATESTime : 3 hrsMax. Marks : 75I. Answer any FIVE of the following :Draw labeled diagrams wherever necessary1.2.3.4.5.6.7.8.II. Answer any FIVE of the following:Draw labeled diagrams wherever necessary9.5x5 255x10 50OR10.OR11.OR12.OR13.OR

ZOOLOGY PRACTICAL SYLLABUS FOR I SEMESTERZOOLOGY - PAPER - IANIMAL DIVERSITY - NONCHORDATESPeriods: 24Max. Marks: 50Observation of the following slides / spotters / modelsProtozoa: Elphidium, Paramecium - Binary fission and conjugationPorifera: Spoonbill, Euspongia, Sycon, Sycon - T.S and L.SCoelenterata: Obelia - colony and medusa, Physalia, Velella, Corallium, Gorgonia,PennatulaPlatyhelminthes: Planaria, Fasciola hepatica, Fasciola larval forms - Miracidium, Redia,Cercaria, Echinococcus granulosusNemathelminthes: Ascaris - Male and female, Ancylostoma duodenaleAnnelida: Neries, Heteroneries, Aphrodite, Hirudo, Trochophore larvaArthropoda: Mouth parts of male and female Anopheles and Culex, Mouth parts ofhousefly, Mouth parts of Scorpion, Nauplius, Mysis, Zoea larvae, crab,prawn, Scolopendra, Sacculina, Limulus, PeripatusMollusca: Chiton, Murex, Sepia, Loligo, Octopus, Nautilus, Glochidium larvaEchinodermata: Ophiothrix, Echinus, Clypeaster, Cucumaria, Antedon, Asterias,Bipinnaria larvaHemichordata: Balanoglossus, Tornaria larvaDemonstration of dissection / dissected / virtual dissection :1. Leech / Prawn / Scorpion / Crab - Digestive system2. Prawn - Appendages3. Prawn / Scorpion / Crab - Nervous system4. Pila / Unio - Digestive system5. Mounting of Statocyst6. Mounting of Radulab Laboratory record work shall be submitted at the time of practical examinationb Compulsory one species to be adopted for demonstration only by the facultyb Computer aided techniques should be adopted as per UGC guide lines

ZOOLOGY SYLLABUS FOR II SEMESTERZOOLOGY - PAPER - IIANIMAL DIVERSITY - CHORDATESPeriods:60Max. Marks: 100Unit - I1.11.2General characters of ChordataProchordata1.2.1 Salient features of Cephalochordata1.2.2 Structure of Branchiostoma1.2.3 Affinities of Cephalochordata1.2.4 Salient features of Urochordata1.2.5 Structure and life history of Herdmania1.2.6 Significance of Retrogressive metamorphosisUnit - II2.12.2Cyclostomata2.1.1 General characters of Cyclostomata2.1.2 Comparision of the Petromyzon and MyxinePisces2.2.1 General characters of Fishes2.2.2 Classification of fishes up to sub - class level with examples2.2.3 Scoliodon - External features, Digestive system,Heart, Brain2.2.4 Migration in Fishes2.2.5 Types of Scales2.2.6 DipnoiRespiratorysystem,Unit - III3.1Amphibia3.1.1 General characters of Amphibian3.1.2 Classification of Amphibia upto orders with examples.3.1.3 Rana hexadactyla - External features, Digestive system, Respiratory system, Heart,Brain3.2Reptilia3.2.1 General characters of Reptilia3.2.2 Classification of Reptilia upto orders with examples3.2.3 Calotes - External features, Digestive system, Respiratory system, Heart, Brain3.2.4 Identification of Poisonous snakes and Skull in reptiles

Unit - IV4.1Aves4.1.1 General characters of Aves4.1.2 Classification of Aves upto subclasses with examples.4.1.3 Columba livia - External features, Digestive system, Respiratory system,Heart, Brain4.1.4 Migration in Birds4.1.5 Flight adaptation in birdsUnit - V5.15.25.3Mammalia5.1.1 General characters of Mammalia5.1.2 Classification of Mammalia upto sub - classes with examplesComparision of Prototherians, Metatherians and EutheriansDentition in mammals

ZOOLOGY MODEL PAPER FOR II SEMESTERZOOLOGY - PAPER - IIANIMAL DIVERSITY - CHORDATESTime: 3 hrsI. Answer any FIVE of the following:Draw labeled diagrams wherever necessary1. Amphioxus2. Placoid scale3. Quill feather4. Prototheria5. Anadromous migration6. Draco7. Emu8. ApodaII. Answer any FIVE of the following:Draw labeled diagrams wherever necessary9. Explain the life history of HerdmaniaORExplain the origin and general characters of chordates10. Compare the characters of Petromyzon and MyxineORDescribe the structure of heart of Scoliodon11. Describe the brain of Rana hexadactylaORExplain the external features of Calotes12. Write an essay on flight adaptations in birdsORExplain the respiratory system of Columba livia13. Compare the characters of Metatheria and EutheriaORWrite an essay on dentition in mammalsMax. Marks: 755x5 255x10 50

ZOOLOGY PRACTICAL SYLLABUS FOR II SEMESTERZOOLOGY - PAPER - IIANIMAL DIVERSITY - CHORDATESPeriods: 24Max. Marks: 50Observation of the following slides / spotters / modelsProtochordata: Herdmania, Amphioxus, Amphioxus T.S. through pharynxCyclostomata: Petromyzon, MyxinePisces: Pristis, Torpedo, Channapleuronectes, Hippocampus,Eheneis, Labeo, Catla, Clarius, Auguilla,ProtopterusExocoetus,Placoid scale, Cycloid scale, Ctenoid scaleAmphibia: Ichthyophis, Amblystoma, Siren, Hyla, RachophousAxolotl larvaReptilia: Draco, Chemaeleon, Uromastix, Vipera russeli, Naja, Bungarus,Enhydrina, Testudo, Trionyx, CrocodilusAves: Passer, Psittacula, Bubo, Alcedo, Columba, Corvus, Pavo, Study ofdifferent types of feathers : Quill, Contour, Filoplume downMammalia: Ornithorthynchus, Tachyglossus, Pteropus, Funambulus, Manis, Loris,HedgehogOsteology: Appenducular skeletons of Varanus, PigeonRabbit - Skull, fore limbs, hind limbs and girdlesDemonstration of dissection / dissected / virtual dissection:1. V, VII, IX, X cranial nerves of shark / locally available fishes2. Arterial system, venous system of Shark / Calotes / Fowl / Rat3. Digestive system of fishb Laboratory record work shall be submitted at the time of practical examinationb Compulsory one species to be adopted for demonstration only be the faculty

ZOOLOGY SYLLABUS FOR III SEMESTERZOOLOGY - PAPER - IIICYTOLOGY, GENETICS AND EVOLUTIONPeriods:60Max. Marks:100Unit - I1.Cytology - I1.1 Definition, history, prokaryotic and eukaryotic cells, virus, viroids, mycoplasma1.2 Electron microscopic structure of eukaryotic cell.1.3 Plasma membrane –Different models of plasma membrane.Unit – II2. Cell organelles2.1 Structure and functions of Endoplasmic Reticulum2.2 Structure and functions of Golgi apparatus2.3 Structure and functions of Lysosomes2.4 Structure and functions of Ribosomes2.5 Structure and functions of Mitochondria2.6 Nucleus2.7. Chromatin - Structure and significance, Chromosomes - Structure, types, functionsUnit - III3.1Genetics - I3.1.1 Mendel’s work on transmission on traits3.1.2 Principles of inheritance3.1.3 Incomplete dominance and codominance3.1.4 Lethal alleles, Epistasis, PleiotropyUnit - IV4.1Genetics - II4.1.1 Sex determination4.1.2 Sex linked inheritance4.1.3 Linkage and crossing over4.1.4 Extra chromosomal inheritance4.1.5 Human karyotypingUnit - V

5.1Evolution5.1.1 Origin of life5.1.2 Lamarckism, Darwinism, Neo – Darwinism, Hardy-Weinberg Equilibrium.5.1.3 Variations, isolating mechanisms, natural selection5.1.4 Types of natural selection (directional, stabilizing, disruptive)5.1.5 Artificial selection and forces of evolution5.1.6 Speciation (Allopatric and Sympatric)5.1.7 Macro evolutionary principles (Example: Darwin’s finches)ZOOLOGY MODEL PAPER FOR III SEMESTERZOOLOGY - PAPER - IIICYTOLOGY, GENETICS AND EVOLUTIONTime: 3 hrsMax. Marks: 75I. Answer any FIVE of the following:Draw labeled diagrams wherever necessary1.2.3.4.5.6.7.8.II. Answer any FIVE of the following:Draw labeled diagrams wherever necessary9.5x5 255x10 50OR10.OR11.OR12.OR

13.ORZOOLOGY PRACTICAL SYLLABUS FOR III SEMESTERZOOLOGY - PAPER - IIICYTOLOGY, GENETICS AND EVOLUTIONPeriods: 24Max. Marks: 50I. Cytology1. Preparation of temporary slides of Mitotic divisions with onion root tips2. Observation of various stages of Mitosis and Meiosis with prepared slides3. Mounting of salivary gland chromosomes of ChiranomousII. Genetics1. Study of Mendelian inheritance using suitable examples2. Study of linkage recombination, gene mapping using the data3. Study of human karyotypesIII. Evolution1. Study of fossil evidences2. Study of homology and analogy from suitable specimens and pictures3. Phylogeny of horse with pictures4. Darwin’s finches (pictures)5. Visit to natural history museum and submission of report

ZOOLOGY SYLLABUS FOR IV SEMESTERZOOLOGY - PAPER - IVEMBRYOLOGY, PHYSIOLOGY AND ECOLOGYPeriods:601.11.21.31.4Max. Marks: 100Unit - IDevelopmental Biology and Embryology1.1.1 Gametogenesis1.1.2 Fertilization1.1.3 Types of eggs1.1.4 Types of cleavagesDevelopment of Frog upto formation of primary germ layersFormation and functions of Foetal membrane in chick embryoDevelopment, types and functions of Placenta in mammalsUnit - II2.13.1Physiology - I2.1.1 Elementary study of process of digestion2.1.2 Absorption of digested food2.1.3 Respiration - Pulmonary ventilation, transport of oxygen and carbondioxide2.1.4 Circulation - Structure and functioning of heart, Cardiac cycle2.1.5 Excretion - Structure of nephron, urine formation, counter current mechanismUnit - IIIPhysiology - II3.1.1 Nerve impulse transmission - Resting membrane potential, origin andpropagation of action potentials along myelinated and non-myelinated nervefibers3.1.2 Muscle contraction - Ultra structure of muscle fibre, molecular andchemical basis of muscle contraction3.1.3 Endocrine glands - Structure, secretions and the functions (of hormones)of pituitary, thyroid, parathyroid, adrenal glands and pancreas3.1.4 Hormonal control of reproduction in a mammalUnit - IV4.1Ecology - I4.1.1 Meaning and scope of Ecology4.1.2 Important abiotic factors of Ecosystem - Temperature, light, water, oxygen and CO24.1.3 Nutrient cycles - Nitrogen, carbon and phosphorus

4.1.4 Components of Ecosystem (Example:lake),energy flow in ecosystemfood chains and food web,Unit - V5.1Ecology - II5.1.1 Habitat and ecological niche5.1.2 Community interactions - Mutualism, commensalism, parasitism, competition,predation5.1.3 Ecological succession5.1.4 Population studies5.2Zoogeography5.2.1 Zoogeographical regions5.2.2 Study of physical and faunal peculiarities of Oriental, Australian and EthiopianregionsZOOLOGY MODEL PAPER FOR IV SEMESTERZOOLOGY - PAPER - IVEMBRYOLOGY, PHYSIOLOGY AND ECOLOGYTime: 3 hrsMax. Marks: 75I. Answer any FIVE of the following:Draw labeled diagrams wherever necessary1.2.3.4.5.6.7.8.II. Answer any FIVE of the following:Draw labeled diagrams wherever necessary9.5x5 255x10 50OR10.OR

11.OR12.OR13.ORZOOLOGY PRACTICAL SYLLABUS FOR IV SEMESTERZOOLOGY - PAPER - IVEMBRYOLOGY, PHYSIOLOGY AND ECOLOGYPeriods: 24Max. Marks: 50I. Embryology1. Study of T.S. of testis, ovary of a mammal2. Study of different stages of cleavages (2, 4, 8 cell stages)3. Study of chick embryos of 18 hours, 24 hours, 33 hours and 48 hours of incubationII. Physiology1. Qualitative tests for identification of carbohydrates, proteins and fats2. Qualitative tests for identification of ammonia, urea and uric acid3. Study of activity of salivary amylase under optimum conditions4. Study of prepared slides of T.S. of duodenum, liver, lung, kidney, spinal cord, boneand cartilageIII. Ecology1. Determination of pH of given sample2. Estimation of dissolved oxygen of given sample3. Estimation of total alkalinity of given sample4. Estimation of salinity of given sample

ZOOLOGY SYLLABUS FOR V SEMESTERZOOLOGY - PAPER - VANIMAL BIOTECHNOLOGYPeriods:60Max. Marks:100Unit 1: Tools of Recombinant DNA technology - Enzymes and VectorsRestriction modification systems: Types I, II and III. Mode of action, nomenclature, applicationsof Type II restriction enzymes in genetic engineeringDNA modifying enzymes and their applications: DNA polymerases. Terminal deoxynucleotidyltransferase, kinases and phosphatases, and DNA ligasesCloning Vectors: Plasmid vectors:pBR and pUC series, Bacteriophage lambda and M13 basedvectors, Cosmids, BACs, YACs,Unit 2 Techniques of Recombinant DNA technologyCloning: Use of linkers and adaptorsGene delivery: Microinjection, electroporation, biolistic method (gene gun), liposome and viralmediated deliveryPCR: Basics of PCR.DNA Sequencing: Sanger’s method of DNA sequencing- traditional and automated sequencingHybridization techniques: Southern, Northern and Western blotting,Genomic and cDNA libraries: Preparation and usesUNIT 3 Animal Cell TechnologyCell culture media: Natural and SyntheticCell cultures: primary culture, secondary culture, continuous cell lines; Protocols for Primary CellCulture; Established Cell lines (common examples such as MRC, HeLa, CHO, BHK, Vero); Organculture; Cryopreservation of cultures.Hybridoma Technology: Cell fusion, Production of Monoclonal antibodies (mAb), Applicationsof mAbStem cells: Types of stem cells, applicationsUnit 4 Reproductive Technologies & Transgenic AnimalsManipulation of reproduction in animals: Artificial Insemination, In vitro fertilization , superovulation, Embryo transfer, Embryo cloningTransgenic Animals: Strategies of Gene transfer; Transgenic - sheep, - fish; applicationsUnit 5 Applied BiotechnologyIndustry: Fermentation: Different types of Fermentation: Short notes on - Submerged & Solidstate; batch, Fed batch & Continuous; Stirred tank, Air Lift, Fixed Bed and Fluidized; Downstreamprocessing - Filtration, centrifugation, extraction, chromatography, spray drying and lyophilizationAgriculture: fisheries – monoculture in fishes, polyploidy in fishes; DNA fingerprinting

ZOOLOGY MODEL PAPER FOR V SEMESTERZOOLOGY - PAPER - VANIMAL BIOTECHNOLOGYTime: 3 hrsI. Answer any FIVE of the following:Draw labeled diagrams wherever necessary1.2.3.4.5.6.7.8.II. Answer any FIVE of the following:Draw labeled diagrams wherever necessary9.Max. Marks: 755x5 255x10 50OR10.OR11.OR12.OR13.OR

ZOOLOGY PRACTICAL SYLLABUS FOR V SEMESTERZOOLOGY - PAPER - VANIMAL BIOTECHNOLOGYPeriods: 24Max. Marks: 50Any SIX of the following:1. Maintenance and storage ofE.coli DH5 alpha cells.2. Isolation of Plasmid DNA from E.coli3. Preparation of genomic DNA from E. coli/animals/ human.4. DNA quantification using agarose gel electrophoresis (by using lambda DNA as standard).5. Restriction digestion of lambda (λ) DNA using EcoR1 and Hind III.6. Preparation for insertion and vector for ligation.7. Performance of ligation reaction using T4 DNA ligase.8. Preparation of competent cells9. Transformation of E. coli with plasmid DNA using CaCl2,10. Selection of transformants on X-gal and IPTG11. Techniques: Western Blot, Southern Hybridization, DNA Fingerprinting12. Interpretation of sequencing gel electropherograms13. Amplification of DNA by PCR14.Packing and sterilization of glass and plastic wares for cell culture.15,Preparation of culture media.SUGGESTED READING1. Brown TA. (2010). Gene Cloning and DNA Analysis. 6th edition. Blackwell Publishing, Oxford,U.K.2. Clark DP and Pazdernik NJ. (2009). Biotechnology: Applying the Genetic Revolution. ElsevierAcademic Press, USA3. Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7thedition. Blackwell Publishing, Oxford, U.K.4. Sambrook J and Russell D. (2001). Molecular Cloning-A Laboratory Manual. 3rd edition. ColdSpring Harbor Laboratory Press5. Wiley JM, Sherwood LM and Woolverton CJ. (2008). Prescott, Harley and Klein’sMicrobiology. McGraw Hill Higher Education6. Brown TA. (2007). Genomes-3. Garland Science Publishers7. Primrose SB and Twyman RM. (2008). Genomics: Applications in human biology. BlackwellPublishing, Oxford, U.K.8. Animal Cells Culture and Media, D.C. Darling and S.J. Morgan, 1994.BIOS ScientificPublishers Limited.9. Methods in Cell Biology, Volume 57, Jennie P. Mathur and David Barnes, 1998. Animal CellCulture Methods Academic Press.10. P.K. Gupta: Biotechnology and Genomics, Rastogi publishers (2003).11. B.D. Singh: Biotechnology, Kalyani publishers, 1998 (Reprint 2001)

ZOOLOGY SYLLABUS FOR V SEMESTERZOOLOGY - PAPER - VIANIMAL HUSBANDRYPeriods:60UNIT – IMax. Marks: 100:10 HoursGeneral introduction to poultry farming. Principles of poultry housing. Poultry houses. Systemsof poultry farming. Management of chicks, growers and layers. Management of Broilers.UNIT – II:10 HoursPoultry feed management – Principles of feeding. Nutrient requirements for different stages oflayers and broilers. Methods of feeding. Poultry diseases – viral, bacterial, fungal and parasitic(two each); symptoms, control a

1.2.1 Salient features of Cephalochordata 1.2.2 Structure of Branchiostoma 1.2.3 Affinities of Cephalochordata 1.2.4 Salient features of Urochordata 1.2.5 Structure and life history of Herdmania 1.2.6 Significance of Retrogressive metamorphosis Unit - II 2.1 Cyclostomata 2.1.1 General characters of Cyclostomata

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