Question Bank Multiple Choice - Virginia Tech

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Question BankMultiple choice1. The desire to maintain a safe laboratory environment for all begins with ?a. preventionc. microbiologyb. ubiquityd. accidents2. When a chemical splashes in the eye rinse for ?a. 10 secondsc. 5 minutesb. 30 secondsd. 15 minutes3. Which of the following type(s) of Personal Protective Equipment (PPE) is frequentlyused?a. Safety glassesd. Glovesb. Lab Coatse. All of the abovec. Face Shields4. Chemical, reagents or broth cultures should be pipetted by ?a. mouthc. pipetterb. eard. nose5. Good work practices include,a. smelling and tasting chemicalsb. not washing hands before and after labc. confining long hair and loose clothingd. using damaged equipment and glassware.6. What is the name of the procedure performed under sterile conditions to eliminatecontamination in hopes to obtain a pure culture of one type of microorganism?a. sterilization techniquec. disinfectant techniqueb. aseptic techniqueb. pathogen technique

7. After a biohazard spill is covered with paper towels and disinfectant solution, it mustsit for minutes?a. 5c. 60b. 30d. 208. is needed as a source of nutrient for the growth and reproduction ofmicrobes.a. pathogensc. reagentsb. bacteriad. media9. To prevent the contamination of microscopes and surrounding areas disenfect/cleanused slides, prepared by student, witha. 70% ethanol and lens paperb. acetone and lens paperb. 5% methylene blue and lens paperd. water and lens paperTrue/False1. Virulent strains of microorganisms are used in your laboratory?2. You should always wash your hands before and after lab.3. Food and drinks are allowed in the lab.4. Lab coats much be taken off when exiting the lab and entering a non-laboratory area.5. Good Laboratory Practice (GLP) is a method employed in a laboratory setting toprevent contamination, accidents and injuries.

6. Following Good Laboratory Practices should only be used when your instructorreminds the class to do so.7. Cell phones can be used in lab if the instructor is not talking.8. Students must know the location of all safety equipment.9. It is good practice to carry the microscope with one hand.Short Answer1. What are the 3 most common ways accidents or incidents occur in the laboratory?2. Identify one safety violation in the picture.3. What type of solution(s) should be used to disinfect a bench top before and after lab?4. The sign below indicates what type of safety hazard?

5. The sign below indicates what type of safety hazard?6. Identify one safety violation in the picture.

Question Bank AnswersMultiple Choice1. a2. d3. e4. c5. c6. b7. b8. d9. aShort Answer1. Inappropriate use ofchemicals and equipment,Inexperienced students orworkers, Inappropriatebehavior or conduct2. Petri dish and chemicalbottle left open3. Bleach and/or ethanolTrue/False1. false2. true3. false4. true5. true6. false7. false8. true9. false4. Toxic chemical5. Biohazard6. Unorganized work area

Question Bank Multiple choice . 1. The desire to maintain a safe laboratory environment for all begins with _? a. prevention c. microbiology . b. ubiquity d. accidents . 2. When a chemical splashes in the eye rinse for _? a. 10 seconds c. 5 minutes . b. 30 seconds d. 15 minutes . 3.

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