Electronic Supporting Information - Theranostics
Electronic supporting informationAssessing therapeutic potential of magnetic mesoporous nanoassemblies for chemoresistant tumorsLina Pradhan a, d, ‡, B. Thakur b, ‡, R. Srivastava c,, P. Ray b, * , D. Bahadur d, *aCentre for Research in Nanotechnology and Sciences, IIT Bombay, Mumbai, 400076,India.bTata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.cDepartment of Biosciences and Bioengineering, IIT Bombay, Mumbai, 400076, India.dDepartment of Metallurgical Engineering and Materials Science, IIT Bombay, Mumbai, 400076 India.‘‡’ These authors contributed equally.*Corresponding authors. Tel.: 91 22 2740 5119 (P. Ray). Tel.: 91 22 25767632 (D. Bahadur).E-mail addresses: pray@actrec.gov.in (P. Ray), dhirenb@iitb.ac.in (D. Bahadur).1. Results and discussionTEM analysisThe TEM images of MMNA and LMMNA are seen as spherical stucture with an averagediameter of about 73 and 92 nm (size distribution is shown in our pevious study), respectively(ESI Figure S1). ESI Figure S1A shows the aggregate of smaller-sized Fe3O4 nanoparticles, anda single MMNA is magnified in ESI Figure S1B. As shown in ESI Figure S 1C, the lipid coatingon the surface of the MMNA slightly increased the size of the MMNA. ESI Figure S1D shows amagnified image of a single LMMNA and a thin layer of lipid over the MMNA markedasredlines with yellow arrows. This result suggests that the lipid-coated MMNA were successfullysynthesized.1
Figure S1. TEM images of (A) MMNA, (B) Magnified image of single MMNA, (C) Lipidcoated MMNA (LMMNA) and (D) Magnified image of single LMMNA and red lines withyellow arrow marked for thin lipid layer on the surface of MMNA.2
Figure S2. DLS measurement of LMMNA in PBS and DMEM showing mean hydrodynamicdiameter of (A) 157 nm in PBS and (B) 159 nm in 10% FBS supplemented .0120DOXTXL0.000Loading capacity (mg/mg)Encapsulation efficiency (%)Loading efficiency and loading capacity of DOX:TXL-LMMNALMMNA ratio (5:2:2:2)Figure S3. Encapsulation efficiency (%) with loading capacity (mg/mg) of DOX and TXL inLMMNA (5:2:2:2 ratio). All experiments were performed in triplicate.3
Figure S4. Magnetic hysteresis curves of (A) large intestine, (B) lung, (C) spleen, (D) liver, (E)small intestine, (F) stomach, (G) heart, (H) kidney, (I) brain and (J) skin of non-tumored nudemice post administration of DOX:TXL-LMMNA.4
Figure S5. TEM images of uptake and accumulation of nanoparticles in spleen and lung tissuesafter Day 7 treatment. Column (A) both tissues show the localization of the LMMNA into tissueand column (B) magnified image of the area indicated by the yellow box in column (A), showingthe morphology and accumulation of LMMNA.5
Temperature ( C)45376 Oe, 250 kHz403530010203040Time (min)Figure S6. Thermal profile of tumor surface after intratumoral administration of DOX:TXLLMMNA (1 mg) under ACMF.6
Figure S7. In vivo biodistribution using 50 mg/kg of DOX:TXL-LMMNA in theabsence/presence of ACMF at 376 Oe, 250 kHz frequency in A2780S tumor xenografts NUDEmice. (A) Highest magnetization values of vital organ tissues at Day 7 (B) Representativefluorescence images of A2780S tumor xenografts.7
Figure S8. The cell cycle distribution and apoptotic population percentages by PI staining onA2780S and A2780-CisR cell lines. The histograms show the untreated as a control, treated withLMMNA, DOX:TXL and DOX:TXL-LMMNA in the absence and presence of ACMF. In allcases, G1/G2 phase is arrested with LMMNA ACMF, only DOX:TXL, DOX:TXL-LMMNAand DOX:TXL-LMMNA ACMF, respectively.8
Figure S9. Comparison between efficacy of DOX:TXL-LMMNA in the presence of ACMF onA2780S and A2780-CisR tumor xenografts. A. Fold change (normalized with Day 0) inbioluminescence signal (A) and tumor volume (B) between sensitive and resistant tumorxenograft.9
Table S1: Number of mice used in biodistribution studyExperiment Condition UntreatedNumber of miceDays post DOX:TXL-LMMNA adminstrationDay 1Day 2Day 7Day 30Day 60555555Total30Table S2: Number of mice used for estimating In vivo therapeutic efficacy of DOX:TXLLMMNA on A2780 S tumor xenograftsExperiment ConditionNumber of miceUntreated DOX:TXL-LMMNA DOX:TXL-LMMNA ACMF Total55515A total of additional 10 mice, treated with DOX:TXL-LMMNA ACMF where kept for 6 monthfollowup study.Table S3: Number of mice used for estimating In vivo therapeutic efficacy of DOX:TXLLMMNA on A2780-CisR tumor xenograftsExperiment Condition Cisplatin ACMF DOX:TXL ACMFNumber of mice55LMMNA ACMFDOX:TXL-LMMNA ACMF Total552010
Electronic supporting information Assessing therapeutic potential of magnetic mesoporous nanoassemblies for chemo-resistant tumors Lina Pradhan a, d, . ESI Figure S1A shows the aggregate of smaller-sized Fe 3 O 4 nanoparticles, and a single MMNA is magnified in ESI Figure S1B. As shown in ESI Figure S 1C, the lipid coating
applications in drug delivery, tumor imaging, therapy and theranostics, illustrated the progress of clinical translation and made prospects. Key words: nanoparticles, stimuli-responsive, tumor microenvironment, diagnosis, theranostics, clinical . tumor tissues or cancer cells for achieving improved diagnostic and therapeutic efficacy. However .
69. electronic woodpecker 55 70. fish caller 55 71. electronic raindrops 56 72. pencil lead organ 56 73. electronic motorcycle 57 74. machine gun pulse detector 57 75. electronic siren 58 76. chirping bird 58 77. electronic cat 59 78. electronic bird 59 79. "horror movie" sound effect 60 80. electronic organ 60 81. soundmachinei 61 82.
50 Yonsei-ro, Seodaemun-gu, Seoul 120–749, Korea eunkim@yonsei.ac.kr This file includes: Supporting Information Figures S1 to S12 Supplementary discussion S1 to S4 Supporting Information Table S1 to S3 Supporting Video S1 to S4 References S1 to S10
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Electronic Devices used in Electronic Smoking Systems: Devices commonly known as electronic cigarettes are devices designed to heat electronic liquids or tobacco products as an alternative to smoking aerosol products, which may contain or not contain nicotine, inhaled through the mouth. Page 5 of 15 .
4 Electronic Discovery. 2/2/2011. Introduction to Electronic Discovery Electronic Discovery, or "e -discovery", is simply the extension of discovery to include data in . electronic format. referred to as Electronically Stored Information "ESI". Recent University of California at Berkeley Analysis : 93% of data created in 2003 is digital;
In today's informatics world, there are two main terms that are used: Electronic Health Record and Electronic Medical Record. According to the National Alliance for Health Information Technology Electronic Health Record (EHR) is an electronic record of health-related information on an individual that conforms to nationally
An Introduction to Modal Logic 2009 Formosan Summer School on Logic, Language, and Computation 29 June-10 July, 2009 ; 9 9 B . : The Agenda Introduction Basic Modal Logic Normal Systems of Modal Logic Meta-theorems of Normal Systems Variants of Modal Logic Conclusion ; 9 9 B . ; Introduction Let me tell you the story ; 9 9 B . Introduction Historical overview .
