Molecular And Synthetic Biology Solutions - Agilent
Molecular andSynthetic Biology SolutionsEmpowering the synthetic biology revolution— from molecules to measurement
The Next-Generation ofMolecular BiologyThe foundational techniques of molecular biology are changing.Synthetic biology approaches to engineering biological systemsand organisms have driven innovations in both DNA synthesis andassembly. Agilent's products bring these novel tools into the reachof every molecular biology lab, improving the speed and reliabilitywhile reducing the cost of next-gen cloning and mutagenesis.Stratagene LABS. Agilent-Backed Quality.Cutting-edge molecular and synthetic biology solutions to accelerate your research.Since 1984, Stratagene products have been used throughout the academic, industry and government research sectorsin fields spanning molecular biology, genomics, proteomics, drug discovery and toxicology. In 2007, Agilent Technologiesintegrated Stratagene’s labs, which now form the primary research and development branch of Agilent’s genomics division. Genomics2
Molecular and Synthetic Biology SolutionsEmpowering the synthetic biology revolution—from molecules to measurement.ContentsSureVector Next Gen Cloning Kits4Mutagenesis Products8Specialty Cloning Products10Viral Expression Systems13Competent Cells163
SureVector Next-Gen Cloning KitsYour Vision. Your Vectors.SureVector, the world’s first modular vector system, harnesses the power of synthetic biologyto provide quick, user-friendly customization of cloning and expression vectors. In contrast toalternative next-gen cloning technologies, SureVector offers a unique set of standard parts thatcan be assembled into an endless supply of custom vectors—all with a validated assemblysystem you can count on.How does SureVector work?A single SureVector kit contains a set of DNA fragments which are the functional “parts” of most cloning and expressionvectors. These parts can be assembled into any combination desired, resulting in customized vectors. The proprietarySureVector enzymes can assemble up to seven fragments into a circularized plasmid in a single, 20-minute reaction.4
XP2 EXPANSIONXP2 LinkerNeoRLacI LEU2XP2GOI USER SUPPLIEDPROMOTER-TAGPT7-His (Bacterial)PCMV-His (Mammalian)PGall-His (Yeast)arinofReBACTERIAL REPLICATIONFRAGMENTpUCp15ApBR322MigkeOrrP ro m o t e rXP1XP1 EXPANSIONyARSXP1 Linkerp licatio nS electableMARKER FRAGMENTAmpRkanRChlrRFast, Flexible, Reliable. Rapid custom vector generationLess than a day from design to vector, compared to fourweeks for custom vector servicesprecise assembly Reliable andSureVector is extensively validated to ensure standardparts can be interchanged without loss of functionalityMulti-Organism FunctionalityBacteriaBacterial expression using SureVector’s T7promoter. Pink colonies on the right expressfluorescent protein when T7 is present, whilenegative controls (left) do not. More flexible than traditional systemsAssemble new vectors in your lab as experimentalrequirements change, rather than ordering a new one Control your experimentsTake control of your experiments by troubleshootingyour DNA assembly—not your service provider’sYeastThe presence of LEU2 gene inthe SureVector expansion slot(right) allows yeast to grow onleucine deficient media.M8NeoStable010515MammalianStable mammalian cell lines usingthe neomycin resistant fragmentfrom the SureVector kit.5
SureVector Next-Gen Cloning Kits (Continued)Agilent SureVector System Fragments & Kit NumbersPromotersTagsE. coliMammalianYeastT7 (G7515A-B, G7518B-E)CMV (G7516A-B)GAL1 (G7517A-B)Tac (G7515A-B, G7518B-C)SV40 (G7516A-B)CUP1 (G7517A-B)Rhamnose (G7515A-B, G7518C)EF-1α (G7516A-B)ADH1 (G7517A-B)CBP (G7515A-B, G7518E)3xFLAG (G7516A-B)3xFLAG (G7517A-B)DsbA (N-term only) (G7515A)GFP (G7516A-B)GFP (G7517A-B)GST (N-term only)(G7515A, G7518D)HA (C-term only) (G7515B)3xHA (G7516A-B)3xHA (G7517A-B)6xHis (G7516A-B)6xHis (G7517A-B)6xHis (G7515A-B, G7518B-C)c-Myc (G7516A-B)c-Myc (G7517A-B)MBP (N-term only) (G7515A,G7518D)c-Myc (C-term only) (G7515B)SBP (G7516A-B)SBP (G7517A-B)Thioredoxin (C-term only)(G7515B, G7518E)AmpR (G7514A, G7518A-E)AmpR (G7514A, G7518A-E)AmpR (G7514A, G7518A-E)CamR (G7514A, G7518A)CamR (G7514A, G7518A)CamR (G7514A, G7518A)KanR (G7514A, G7518A)KanR (G7514A, G7518A)KanR (G7514A, G7518A)pUC (G7514A, G7518A-E)pUC (G7514A, G7518A-E)pUC (G7514A, G7518A-E)p15A (G7514A)p15A (G7514A)p15A (G7514A)pBR322 (G7514A)pBR322 (G7514A)pBR322 (G7514A)XP1 (G7514A, G7518A-E)XP1 (G7514A, G7518A-E)yARS (G7514A)SBP (G7515A-B, G7518D-E)Bacterial SelectionBacterial Origins of ReplicationXP1 FragmentsXP1 (G7514A, G7518A-E)XP2 FragmentsPromoter-Tag Fusions6LacI (G7514A, G7518A-E)Blasticidin (G7516A-B)URA3 (G7517A-B)XP2 (G7514A)Hygromycin (G7516A-B)HIS3 (G7517A-B)Puromycin (G7516A-B)Hygromycin (G7517A-B)NeoR (G7514A)LEU2 (G7514A)XP2 (G7514A)XP2 (G7514A)CMV-HIS6 (G7514A)GAL1-HIS6 (G7514A)T7-HIS6 (G7514A, G7518A-B,G7518D)
Mutagenesis ProductsEfficiency Without CompromiseFrom rational design to random mutations, Agilent offers mutagenesis solutions for any application.Agilent offers the only widely available commercial technology that is not PCR based, so you don’thave to sacrifice error rate for efficiency.Market-leading QuikChange MutagenesisQuikChange kits have provided researchers with a fast, easy and efficient non-PCR method to reliably perform site-directedmutagenesis since 1996. Other commercially-available kits utilize PCR-based techniques, which can propagate errors with eachsuccessive round of thermal cycling. The QuikChange method uses a linear amplification strategy with only the parental strandserving as the DNA template. Combining this with our highest fidelity polymerases leads to a significant reduction in unwantedsecond-site errors. The existence of such errors is likely to complicate and delay downstream screening and analysis.QuikChange Lightning MultiQuikChange LightningGeneMorph II F ast, reliable and easyQuikChange protocol 7 5% reduction in thermocycling timecompared to original QuikChangeenzyme blend M ore uniform mutational spectrumwhen performing error-prone PCR M utate up to three sitessimultaneously using a singleQuikChange reaction G eneMorph II kits utilize MutazymeII DNA polymerase, a novel errorprone PCR enzyme blend, withequivalent mutation rates at Asand Ts vs. Gs and Cs M ore efficient with improvedcolony yields 80% mutation efficiency for bothshort and long templates (up to 14 kb)The ‘Lightning Advantage’The QuikChange Lightning Kitcontains specially engineeredenzymes that have been designedto shorten the time necessary to complete our signature 3-stepPredominantprotocol. Extension times for theproduct from step 1thermal cycling process have beenreduced by 75% and digestion of thePredominantproduct from step 1non-mutated parental template hasbeen decreased to only five gThermal cyclesThermal cycles Multi1 Mutant Strand SynthesisPerformthermal cycling1 MutantStrandto:Synthesis Denature DNA templatePerformthermal Annealmutagenicprimers cycling to: DenatureDNAtemplate(all primersbind to thesamestrand) Extendligate nicks primerswith primersAnnealandmutagenicQuikChange Multi enzyme2(all primers bind to the same strand) Extend primers and ligate nicks withDpn I Digestionof TemplateQuikChangeMulti enzyme Digest methylated and hemimethylatedDNA with Dpn I2 Dpn I Digestion of Templatehemimethylated3 Transformation Digest methylated andTransform mutated ssDNA intowith Dpncells,I whichXL10-GoldDNAultracompetentsynthesize the complementary strand3 TransformationTransform mutated ssDNA intoXL10-Gold ultracompetent cells, whichsynthesize the complementary strand7
Mutagenesis Products (Continued)QuikChange HT Protein Engineering SystemQuikChange technology meets high-throughput DNA synthesis to provide access to rationally-designed oligo libraries forprotein engineering applications. The QuikChange HT Protein Engineering System provides rapid resolution of structuraland functional questions by creating libraries of rationally-designed mutants for applications such as single amino acidscanning, site saturations scanning or targeted combinatorial mutagenesis.Key Features:Use QuikScan1 to determinerelevant stability: Separatelyreplaces each amino acid in thewild type mutational region witha particular amino acid. Oftenused for Alanine scanning toquickly identify key functionalor structural amino acids. Rapidly generate a rational design library of proteinvariants—less than a full day of hands-on time comparedto weeks of waiting for a gene variant library Reduced cost of library generation—only pennies per mutantcompared to 20 or more for gene variant librariesUse QuikScan19 to identify single codonreplacements that improve binding,function or stability: Codon saturationscanning, systematically replaces eachamino acid in the wild type mutationalregion with all 19 other amino acids,resulting in 19 mutagenic oligos foreach amino acid position in themutational region.QC HT MethodsSub-Set1# Clones 7β8β9Y124VVCβ 94PG213NG233S*N116E11121314151617181Mutational region 1MutationOligoset 1PCR primer annealing sitesMutationOligoset 250AA x 19mut 950oligos 1 QuikChange reactionL184RKSSTTEM121EEQ R125HDK3β 10TargetSequence91 101 111 121 131 141 151 161 171 181 191 201 211 221 231Use QuikCombine to discover a multisitemutant with improved structure, functionand stability: Combine multiple mutantsin groups of 1–4 position with definedvariation at each site. Make up to 1.2x104libraries for a single 50AA set or combine Oligoset 3a few identified variants and validatefunctional relevance.Mutational region 3hrGFP aa positionAn example of the QuikChange HT kit applied to engineering of a GFP variantwith enhanced brightness. Using site saturation mutagenesis yielded severalbeneficial mutations.ProductThree possible mutational strategies using QuikChange HT: Alaninescanning, site saturation scanning and combinatorial mutagenesis.UsesPart NumberQuikChange MutagenesisQuikChange Lightning MultiUse for up to 3 mutations simultaneously, 10 or 30 reaction kitsQuikChange LightningSingle site mutagenesis, 10 or 30 reaction kits210514,210516210518,210519QuikChange HT Protein Engineering SystemQuikChange HTUse for targeting up to 10 different 50 amino acid long regions in a proteinG5900AQuikChange HTUse for targeting up to 20 different 50 amino acid long regions in a proteinG5900BQuikChange HTUse for targeting up to 10 different 67 amino acid long regions in a proteinG5901AQuikChange HTUse for targeting up to 20 different 67 amino acid long regions in a proteinG5901BMutagenic polymerase for balanced random mutagenesis200550,200552Random MutagenesisGeneMorph II8
Specialty Cloning ProductsA Solution for Every SituationWhen you have a difficult cloning project, Agilent offers everything from a traditional topoisomerasebased kit to a huge selection of catalog vectors for any application.StrataClone PCR Cloning KitThe StrataClone PCR Cloning Kit allows high-efficiency,5-minute cloning of PCR products at room temperature,using the efficient DNA rejoining activity of DNAtopoisomerase I and the DNA recombination activityof Cre recombinase. These kits are available for bothblunt-end and UA cloning.Incubate blunt PCR product withTopoisomerase I-chargedvector arms (5 minutes)1PCR ProductCotransfectTopoisomerase IPromoterPromoterGAL4 dbddbdGAL4PromoterPromoterGene ofof interestinterestIoxP GenepUC oriP facGAL4 UASUASGAL4Pathway-specific fusionfusionPathway-specifictrans-activator porter EnzymeEnzymeReporterMCSIacZamp/kanIoxPReporter plasmidplasmidReporterTopoisomerase IThe blunt end StrataClone kit is perfect for use with our new Cas9 programmable restrictionenzyme kit. Cas9 can be used to produce a linear fragment of DNA with blunt ends that can berapidly cloned into the StrataClone vector.IacZActivator domainpUC oriProtein of interest phosphorylates pathway-specificcZfusion trans-activator protein either directly or indirectlyIaPCRProductSMC2c MCSP laStrataClonePCR Cloning VectorpSC-B-amp/kanamycinxPIoPathDetect Cis and Trans-Reporting SystemsDetermine if a gene product or compound activates pathways leadingtoProtein of interestspecific enhancers with our PathDetect Cis and Trans-Reporting systems.13CotransfectOHOHGAL4dbdGAL4dbdPO4PO4ampi c illi n/kanPhosphorylated pathway-specificpathway-specific fusionfusion n bindsbinds asas aa dimerdimer withwith GAL4GAL4 UASUAS andand activatesactivatesproteintranscription ofof thethe reporterreporter AL4 dbdGeneGene ofof interestinterestGAL4GAL4 UASUASPathway-specific fusiontrans-activator orterReporterplasmidplasmidPO44 PO44GAL4GAL4 UASUAS2Protein of interestinterest phosphorylatesphosphorylates pathway-specificpathway-specificfusion trans-activatortrans-activator proteinprotein eithereither directlydirectly oror nzymeAssay forfor ReporterReporter EnzymeEnzymeAssayActivatorActivator domaindomainPO44 PO44ProteinProtein ofof interestinterestOHOHGAL4dbdGAL4dbdPOPO44GAL4GAL4 UASUASTATATAReporterReporterEnzymeEnzymeThe PathDetect in vivo signal transduction pathway trans-reporting system.3PhosphorylatedPhosphorylated pathway-specificpathway-specific fusionfusion trans-activatortrans-activatorproteinprotein bindsbinds asas aa dimerdimer withwith GAL4GAL4 UASUAS andand activatesactivatestranscriptiontranscription ofof thethe reporterreporter enzymeenzyme9
Specialty Cloning Products (Continued)InterPlay TAP Systems for Protein-Protein InteractionsThe InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. Tandem affinitypurification yields your tagged protein and interacting proteins using gentle washing and small molecule elution conditions.Two Easy Purification StepsTo purify proteins with the TAP protocol, apply the mammalian cell lysate to the streptavidin resin, then elute using biotin,and apply that eluate to a calmodulin resin. Once you elute with EGTA, you will get exceptionally clean proteins.Streptavidin-bindingpeptide (SBP)Protein of interest withinteracting partnerKey:Calmodulin-bindingpeptide (SBP)ContaminantsCalmodulin resinStreptavidin resinSpecialty VectorsampicillinSuperCos 1LacSwitch IISuperCos 1 is a novel, 7.9 kb cosmid vector thatcontains bacteriophage promoter sequencesflanking a unique cloning site.The LacSwitch IIinducible mammalianexpression systemutilizes an improvedvector system in whichseveral elements ofthe lac operon havebeen modified for usein eukaryotic cellsfor inducible geneexpression.BamH Iampicillincos sitecos recognition sequenceXba Icos sitepUC oriSuperCos 1cos recognition sequence7.9 kbneomycinP TKpOPRSVI/MCS5.6 kbpUC orif1 ori3’-splice site lacO 1P RSVTK pA5’-splice siteMCSlacO 2SV40 intronampicillinneomycinP TKpOPI3CATpUC ori6.3 kbf1 oriP SV40neomycin3’-splice site3x lacOTK pAWe have a vector system for any application you could imagine —visit www.genomics.agilent.com10P RSV5’-splice siteCATSV40 intron
ProductPart NumberStrataClone SystemsProductPart NumberPath Detect Cis-Reporting SystemsStrataClone PCR Cloning Kit240205AP-1 cis-Reporting System219073StrataClone Blunt Cloning Kit240207NF-κB cis-Reporting System219077StrataClone Ultra Blunt Cloning Kit240218SRF cis-Reporting System219081ISRE cis-Reporting System219092NFAT cis-Reporting System219094Trans-Reporting SystemsPathDetect c-Jun trans-Reporting System219000C/EBP cis-Reporting System240111PathDetect Elk1 trans-Reporting System219005DR3 cis-Reporting System240115PathDetect CREB trans-Reporting System219010Egr-1 cis-Reporting System240129PathDetect CHOP trans-Reporting System219015GRE cis-Reporting System240133pFA-ATF2 Plasmid219026pAP-1-hrGFP Plasmid240049pFA-cFos Plasmid219031pNF-κB-hrGFP Plasmid240051pFA-CMV Plasmid219036pLuc-MCS Plasmid219087pFR-CAT Plasmid219001CRE cis-Reporting System219075pFR-βGal Plasmid219002SRE cis-Reporting System219079pFR-SEAP Plasmid219004p53 cis-Reporting System219083pFA-CHOP Plasmid219054GAS cis-Reporting System219093pFA2-CREB Plasmid219068TARE cis-Reporting System219095pFA2-Elk1 Plasmid219062DR1 cis-Reporting System240113pFA2-cJun Plasmid219053DR5 cis-Reporting System240119pFR-Luc Plasmid219050LILRE cis-Reporting System240131DR4 cis-Reporting System240135pCRE-hrGFP Plasmid240050pNFAT-hrGFP Plasmid240053InterPlay TAP Systems for Protein-Protein InteractionsInterPlay N-Terminal Mammalian TAPSystem Kit240103InterPlay C-Terminal Mammalian TAPSystem Kit240104InterPlay N-Terminal Mammalian TAP Vectors,3 x 20 µg240101InterPlay C-Terminal Mammalian TAP Vectors,3 x 20 µg240102InterPlay Mammalian TAP Purification Kit240107InterPlay Adenoviral N-terminal TAP240213Interplay Adenoviral C-terminal TAP240215InterPlay N-Terminal Mammalian TAP Vectors,3 x 20 µg240214InterPlay C-Terminal Mammalian TAP Vectors,3 x 20 µg240216Specialty VectorsSuperCos (10 rxn kit)251301LacSwitch II system217450Additional components for Path Detect Cis-ReportingSystems can be found at www.genomics.agilent.com11
Viral Expression SystemsHigh-Efficiency Gene Delivery Starts HereAs synthetic biology moves out of the prokaryote and into eukaryotic systems, the need tostudy gene expression in a native host is becoming increasingly important. Many of thesehosts are difficult or impossible to transfect, meaning progress may be limited by hosts thateasily accept DNA using traditional transfection methods. To solve this problem, viral-basedgene delivery systems have been developed for exceptionally high-efficiency gene delivery toa broader range of hosts.ApplicationLong-Term GeneExpressionTransient, High-LevelGene ExpressionFunctional CloningAssaysSystemAAV Helper-FreeSystemAdEasy AdenoviralSystemsViraPort RetroviralExpression System I nfects both dividing andnon-dividing cells L ong-term, stable geneexpression U nparalleled biosafety profile H igh-level protein production I nfects both dividing andnon-dividing cells H omologous recombinationin E. coli saves weeks of work I ntegrates into host genomefor stable expression C opy number controlled bymultiplicity of infection F unctionally screen cDNAlibraries in mammalian cells P re-made libraries availablehrGFPhGH pApAAV-IRES-hrGFP Cloning Vectorrip f1 oriamβ-globin intron MCShGH pApHelperpAAV-MCS Cloning Vectorβ-globin intronhrGFPriCopUE4hGH pAamplacZSV40 pApAAV-RCf1 oripAAV-lacZ Control VectorA AVpCMV-MCSpApL - ITampamP CMVcn intron MCSlobiβ-gR f1 oriR-ITpAAV Vectors-2PCMVorif1hGHRpU12ep2rV-pAAV-hrGFP Control VectorhGH intronAAapMCS3x FLAG IRESAE2The AAV Helper-Free Systemimproves upon recombinantadeno-associated virus-2 (AAV-2)technology by eliminating theneed for helper virus. It allowssafe, high-efficiency gene deliveryand long- term expression in abroad range of hosts.β-globin intronoAAV Helper-FreeVA pUCAdvantagesCo riampp U C o ri
pShuttle-CMV-lacZ VectorP CMVMCS 3x HAhrGFPIRESSV40 pApShuttle-IRES-hrGFP-2 VectorP CMVMCS 3x FLAGhrGFPIRESSV40 pApShuttle-IRES-hrGFP-1 VectorP CMVMCS SV40 pAAdEasy XL and AdEasy SystemspShuttle-CMV VectorThe AdEasy XL and AdEasy Adenoviral Vector Systems save you aMCSmonth of work over traditional methods by producing the recombinantadenoviral plasmid by homologousrecombination in E. coli. Now youpShuttle Vectorcan obtain your recombinant plasmid after a simple transformation.P CMVESP CMVSV40 pAlacZpShuttle-CMV-lacZ VectorP CMVMCS 3x HASV40 pAhrGFPIRESpShuttle-IRES-hrGFP-2 VectorMCS 3x FLAGSV40 pAhrGFPIRESNumber of DaysAdEasy XLSystemoloc1Pa -ITRLAdEasy pShuttle VectorspShuttle-IRES-hrGFP-1 Vector49P CMVMCSepBKey:Clone geneof interestgyPac 1R-ITRPmTraditionalSystemMCS SV40 pApShuttle-CMV Vector1TM39gy16ri g h t a r m h o m6kan3R322o rilomohoObtain recombinedleft ar madenoviral vectorpShuttle VectorVirusproductiongyESpAdEasy-1 VectoroloAdEasy pShuttle VectorsViraPortOur ViraPort retroviral gene expression system is superior to standardtransfection technology. High transduction efficiency and large cloningcapacity (up to 8 kb) make the system ideal for building and screeningcomplex libraries.PmogypByR322o rihleft ar momolpAdEasy-1 VectororiE 1/molo g yAd5 (E3-delteampR322edpBhoViraPack Transfection Kitmet a r m h o m ol o gftarelm-dleri g hPac 1R-ITRho22arE3R3emooriE 1/d)pB1lo g yAd5 (teampri g h t a r m h o mkanc1Pa -ITRLViraPort)leftSystemAAVAdEasyTM XLGene delivery efficiency 90% 90% 90% 20%Host: Dividing cells Host: Non-dividing cells --Long-term expression - Transient expression- - High-titer virus -N/AHost immunogenecity- -N/AMaximum insert size3 kb7.5 kb 8 kbVariableSelection for stable cells /-N/A- ri g hTransfectiongyt a r m h o m ol o13
Viral Expression Systems (Continued)ProductQuantityPart NumberAAV Helper-Free SystemProductQuantityPart NumberAdEasy and AdEasy XL Adenoviral Vector SystemsAAV Helper-Free System pAAV-MCS vector, 10 µg pCMV-MCS vector, 10 µg pAAV-lacZ vector, 10 µg pAAV-RC vector, 20 µg pHelper vector, 20 µg AAV-293 cells, 1x106 cells AAV HT1080, 1x106 cells1 kit240071pAAV-hrGFP Vector20 µg240074pAAV-IRES-hrGFP Vector20 µg240075AAV-293 Cells1 x 106 cells240073AAV-HT1080 Cells1 x 106 cells240109ProductQuantityPart NumberAdEasy XL System pShuttle vector, 20 µg pShuttle-CMV vector, 20 µg pShuttle-CMV-lacZ control vector,10 µg BJ5183-AD1 electroporationcompetent cells, 5 x 100 µl XL10-Gold ultracompetent cells,5 x 100 µl pUC18 DNA control plasmid, 10 µl AD-293 cells, 1 x 106 cells1 kit240010BJ5183-AD1 electroporationcompetent cells5 x 100 µl2001571 kit240009pFB Retroviral Vector10 µg217563pFB-Neo Retroviral Vector10 µg217561AdEasy Adenoviral Vector System pAdEasy-1 vector, 2.5 µg pShuttle vector, 20 µg pShuttle-CMV vector, 20 µg pShuttle-CMV-lacZ vector, 10 µg BJ5183 electroporation-competentcells, 5 x 100 µl XL10-Gold ultracompetent cells,5 x 100 µl pUC18 DNA control plasmid, 10 µlpVpack-GP Vector20 µg217566BJ5183 electroporation-competent cells5 x 100 µl200154pVpack-Eco Vector20 µg217569pAdEasy-1 vector2.5 µg240005pVpack-Ampho Vector20 µg217568pShuttle vector20 µg240006pVpack-10A1 Vector20 µg217570pShuttle-CMV vector20 µg240007pVpack-VSV-G Vector20 µg217567pShuttle-CMV-lacZ control vector10 µg240008Vitality pFB-hrGFP plasmid vector10 µg240027pShuttle-IRES-hrGFP-120 µg240081pFB-Neo-lacZ plasmid vector10 µg240029pShuttle-IRES-hrGFP-220 µg240082pFB-Luc plasmid vector10 µg240030ProductQuantityPart Number1 kit200488ViraPort Retroviral Gene Expression System ViraPack Transfection KitViraPack Transfection Kit14
Competent CellsExplore a wider selectionFinding the right competent cells is easy with Agilent—we have a comprehensive selection ofstrains for all your next-generation cloning needs.Cloning CellsExpression CellsThe Highest EfficiencyThe Widest SelectionOur Ultracompetent Cells provide the highesttransformation efficiency in the world, making it easierand faster to obtain an accurate clone. At AgilentTechnologies, we understand the less time you spendworrying about cloning, the more time you can spendanswering your research questions.We aren’t content just to have the best competent cells.Agilent has designed strains for protein expression, plasmidstability, large plasmids and toxic proteins as well as everydaycloning. Our complete line of competent cells includesspecialty cells for a huge variety of applications, each backedby Agilent’s reputation for the best quality in the field.Our cell lines out-perform the competition.Agilent has expression cells designed to workspecifically with the popular T7 promoter system.cfu/µg supercoiled DNA4.5 x 1010T7 RNAPOLYMERASE-BASEDSYSTEMS4.0 x 10103.5 x 10103.0 x 10102.5 x 10102.0 x 10101.5 x 1010 1 x 108BL21-Gold(DE3)pLysScellsEndA- 1 x 108BL21-GoldcellsEndA- 1 x 108BL21-Gold(DE3)cellsEndA- 1 x 106BL21(DE3)pLysScells 1 x 107BL21-CodonPlus -RILcellsEndA- 1 x 106BL21-CodonPlus (DE3)-RIPLcellsEndA- 1 x 107BL21-CodonPlus -RPcellsEndA- 1 x 107BL21-CodonPlus (DE3)-RILcellsEndA- 1 x 106BL21cells 1 x 107BL21-CodonPlus (DE3)-RPcellsEndA-1.0 x 10105.0 x 109Competitor ICompetitor 2Competitor 3ElectroTen-Blue ElectroporationCompetent CellsElectroTen-Blue Cells vs the Competition(Ligated DNA Constructs)Agilent’s comprehensive selection of cell linescovers the entire range of efficiencies.10610710810910101011 1 x 106BL21(DE3)cellssubcloning efficiencycompetent efficiencysupercompetent efficiencyultracompetent efficiencyelectroporation-competentefficiency ElectroTen-Blue cells(transformants/µg of pUC18 DNA)15
Competent Cells ciencyPart NumberCloning CellsSURE 2 Supercompetent CellsSURE Electroporation Competent CellsSURE Competent CellsUnstable clones; DNA withsecondary structureDNA with secondarystructure, difficultDNA with secondarystructure, routine 1 x 109 1 x 1010 5 x 108Tetracycline, Kanamycin,ChloramphenicolTetracycline, Kanamycin,ChloramphenicolTetracycline, Kanamycin,Chloramphenicol200152200227200238ABLE C Electroporation Competent CellsFor toxic clones 5 x 109Tetracycline, Kanamycin200161ABLE K Electroporation Competent CellsFor toxic clones 5 x 109Tetracycline, Kanamycin200162ABLE C Competent CellsFor toxic clones 5 x 106Tetracycline, Kanamycin200171ABLE K Competent CellsFor toxic clones 5 x 106Tetracycline, Kanamycin200172N/A200123 5 x 109Tetracycline andChloramphenicol200314,200315 5 x 109Tetracycline andKanamycin200317TG1 Competent CellsXL10-Gold Ultracompetent CellsXL10-Gold KanR Ultracompetent CellsElectroTen-Blue Electroporation Competent CellsSoloPack Gold Supercompetent CellsSoloPack Gold Competent Cells96Pack Gold Competent CellsFor phage libraries; Phagedisplay librariesLarge plasmids, ligated DNA,or plasmid librariesLarge plasmids, ligated DNA,or plasmid libraries; plasmidswith CamRLigated DNA and generatinglibrariesHigh efficiency, singlereaction formatRoutine cloning, singlereaction formatRoutine cloning, higherthroughput format1 x 1010 3 x 1010 1 x 109 1 x 108 1 x 108Tetracycline andKanamycinTetracycline andChloramphenicolTetracycline andChloramphenicolTetracycline andChloramphenicol200159230350230325200324XL1-Blue ElectroporationCompetent CellsElectroporation 1 x 1010Tetracycline200228XL1-Blue MRF Electroporation Competent CellsElectroporation, MethylatedDNA 1 x 1010Tetracycline200158XL2-Blue Ultracompetent CellsHighest cloning efficiency 5 x 109XL2-Blue MRF Ultracompetent CellsHighest cloning efficiency formethylated DNA 5 x 109XL1-Blue Supercompetent CellsHighest cloning efficiency 1 x 109Tetracycline200236 1 x 109Tetracycline200230 1 x 109Kanamycin200248 1 x 109N/A200229XL1-Blue MRF Supercompetent CellsXL1-Blue MRF Kan Supercompetent CellsXL1-Blue MR Supercompetent CellsHighest cloning efficiency formethylated DNAHighest cloning efficiency formethylated DNA andtetracycline resistantplasmidsFor cloning without the F’episomeTetracycline andChloramphenicolTetracycline andChloramphenicol200150200151XL1-Blue Competent CellsFor routine cloning 1 x 108Tetracycline200249XL1-Blue Subcloning Grade Competent CellsCloning when DNA is notlimited 1 x 106Tetracycline20013016
ProductUsesTransformationResistanceEfficiencyPart Number 5 x 107Tetracycline, Kanamycin200124 5 x 105Tetracycline200134Expression CellsTKX1 CellsTKB1 CellsFor phosphoproteingenerationFor phosphoproteingenerationArcticExpress Competent CellsEnhanced solubility 5 x 106Tetracycline230191ArcticExpress (DE3) Competent CellsEnhanced solubility 5 x 106Tetracycline230192ArcticExpress (DE3) RIL Competent CellsEnhanced solubility 5 x 106Tetracycline230193ArcticExpress (DE3) RP Competent CellsEnhanced solubility 5 x 106Tetracycline230194ArcticExpress RIL Competent CellsEnhanced solubility 5 x 106Tetracycline230195ArcticExpress RP Competent CellsEnhanced solubility 5 x 106Tetracycline230196BL21-CodonPlus (De3)RIPL Competent CellsEliminate codon bias, use with 1 x 106pET or pCALBL21-CodonPlus (De3)RIL Competent CellsBL21-CodonPlus (De3)RP Competent CellsBL21-CodonPlus RIL Competent CellsBL21-CodonPlus RP Competent CellsBL21-CodonPlus (De3) RIL-X Competent CellsBL21-CodonPlus (De3) RP-X Competent CellsBL21-GoldBL21-Gold (De3)BL21-Gold (De3) pLysSBL21Eliminate codon bias, use withpET or pCALEliminate codon bias, use withpET or pCALEliminate codon bias, fornon-T7 expression systemsEliminate codon bias, fornon-T7 expression systemsMethionine auxotroph forx-ray crystallographyMethionine auxotroph forx-ray crystallographyIncreased efficiency andEndA-,use with toxic proteins andnon-T7 systemsIncreased efficiency and EndA-,use with non-toxic proteinsIncreased efficiency andEndA-,use with toxic or non-toxicproteinsUse with non-T7 systems orwith lambda-CE6 for toxicproteins 1 x 107 1 x 107 1 x 107 1 x 107Chloramphenicoland Streptomycin/SpectinomycinTetracycline andChloramphenicolTetracycline andChloramphenicolTetracycline andChloramphenicolTetracycline andChloramphenicol230280230245230255230240230250 1 x 107Tetracycline230265 1 x 107Tetracycline230275 1 x 108Tetracycline230130 1 x 108Tetracycline230132 1 x 108Tetracycline andChloramphenicol230134 1 x 106Tetracycline200133BL21 (De3)Use with non-toxic proteins 1 x 106Tetracycline200131BL21 (De3) pLysSUse with toxic or non-toxicproteins 1 x 106Chloramphenicol200132XL1-Red CellsFor random mutagenesisN/ATetracycline20012917
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For Research Use Only. Not for use in diagnostic procedures.PR7000-0430 Agilent Technologies, Inc. 2017Printed in USA, October 27, 20175991-9163EN
Cutting-edge molecular and synthetic biology solutions to accelerate your research. Genomics The foundational techniques of molecular biology are changing. Synthetic biology approaches to engineering biological systems and organisms have driven innovations in both DNA synthesis and assembly. Agilent's products bring these novel tools into the .
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The journal Molecular Biology covers a wide range of problems related to molecular, cell, and computational biology, including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, and molecular evolution. Molecular Biology publishes reviews, mini-reviews, and experimental and theoretical works .
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pose environmental risks associated with release of synthetic organisms. The standardization and modularization that are distinguishing features of synthetic biology also have dual implications. By lowering costs and skill levels required to practice biological engineering, synthetic biology may allow developing countries and small firms to
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