Medical Imaging - VUB
Medical ImagingVicky CaveliersResearch group Medical Imaging (MIMA)In vivo Cellular and Molecular Imaging Lab (ICMI) - VUBNuclear Medicine Department - UZ Brussel
Molecular Imaging“Molecular imaging is the non-invasive visualization,characterization and measurement of biological processesat the molecular and cellular levels in humans and otherliving systems”Preclinical2Clinical7-12-2011
Bench to bedside back to bench37-12-2011
Clinical Imaging Computed Tomography orCT scan AnatomicalSingle Photon Emission TomographyPositronor PET scanSPECT scan Molecular
ICMI - Preclinical Multimodality 7-12-2011
ICMI - Preclinical Multimodality 67-12-2011
Multi-disciplinary team7 (Radio)chemistry & (Radio)pharmacy Molecular Biology, Bioengineering MD: Radiology & Nuclear Medicine MD: Cardiology Medical Physics, Mathematics, Informatics7-12-2011
In vivo Cellular and Molecular Imaging Lab (ICMI)Probe developmentidentify molecular probes select signalling label(imaging vs therapy) (radio)labeling procedures stability Biomedicalidentify molecular targets select preclinical model in vivo biodistribution andpharmacokinetics specificity and targeting clinical translation Math and Physicsimage reconstruction quantification new technologies MolecularTarget8lahoutte@gmail.com; vicky.caveliers@uzbrussel.be7-12-2011
Overview of molecular targetsOngoing research: Oncology CEA, EGFR, HER2, somatostatin receptor,amino acid transporters, hypoxia Cardiology VCAM1, LOX1 Immunology VCAM1, macrophages, dendritic cells Diabetes 9Beta cells7-12-2011
Overview of molecular probes10 Substrates (18F-FDG) Synthetic molecules (18F-FMISO) Amino acids (123I-Tyr, 18F-FMP, 18F-FET) Peptides (68Ga-octreotide)7-12-2011
Octreotide : from SPECT to PET111In-OctreoscanSPECT/CT68Ga-DOTANOCPET/CT
Overview of molecular probes Substrates (18F-FDG) Synthetic molecules (18F-FMISO) Amino acids (123I-Tyr, 18F-FMP, 18F-FET) Peptides (68Ga-octreotide) Proteins 12Nanobodies7-12-2011
Nanobodies ?CamelidaeHVHHVHCH2FcCH3Classic Monoclonal Antibody(IgG1)Single chain antibodies(IgG2 & IgG3) Heavy and light chains both chains are necessaryfor antigen-binding only heavy chains ! .fully functional forantigen-binding15 kDaNanobody The smallest functionalfragment from a naturalsingle chain AbHamers-Casterman et al., Nature, 1993
Nanobodies as probes for molecular imagingand radionuclide therapy99mTc, 111InγSPECTNanobody68Ga, 18F, 64Cu,receptorcellPETNIRF dyeFLUORESCENCEIMAGE GUIDED SURGERY177Lu, 90Y211Atα(Collaboration with Duke University)RADIONUCLIDE THERAPY147-12-2011
Imaging M2 macrophages intumor micro-environment3LL tumor WT3LL tumor MMR KO mouseconfidentialconfidential157-12-2011
anti-HER 2 lead nanobody for clinicaltranslationHer2167-12-2011
68Ga-NOTA-anti-HER2-Nanobody First in human study: Aim: To evaluate safety, biodistribution and dosimetry To compare biodistribution of 0.01, 0.1 and 1mg To show targeting in HER2 breast cancer Study population Female patients 18y with locally advanced or metastatic HER2 breast carcinoma Study groups 5 patients for each dose (0.01, 0.1 and 1mg)
68Ga-NOTA-anti-HER2-Nanobody Exclusion criteria Pregnancy Anti-HER2 treatment Abnormal liver or kidney function Patients with active infectionsStudy procedure Single IV injection of 68Ga-NOTA-anti-HER2-Nanobody Total body PET/CT scan @ 1h and 2h p.i. (2x25 min) No contrast injections Clinical exam, blood sampling and urine analysis
Futur focus New imaging technologies (PET/MRI,.) andreconstruction algorithms 19Molecular probe development: Further extend Nanobody platform GMP radiopharmaceutical productionTranslational research !7-12-2011
AcknowledgementsProf Tony Lahoutte, PhDProf Michel Defrise, PhDProf Vicky Caveliers, PhDProf Axel Bossuyt, PhD20Post-doc researchersCatarina Xavier, PhDNick Devoogdt, PhDIlse Vaneycken, PhDMarleen Keyaerts, PhDSophie Hernot, PhDJan Heemskerk, PhDChristophe Cloquet, PhDPhD candidatesLode GoethalsInneke WillekensMatthias D’huyvetterSam MassaJens De VosAnneleen BlykersReema Al-AifariCindy Peleman, Laboratory technicianCarlien Geldof, Executive research assistant7-12-2011
Research group Medical Imaging (MIMA) In vivo Cellular and Molecular Imaging Lab (ICMI) - VUB Nuclear Medicine Department - UZ Brussel. 2 7-12-2011 . Medical Physics, Mathematics, Informatics. 8 7-12-2011 In vivo Cellular and Molecular Imaging Lab (ICMI) Probe development identify molecular probes
1 Acoustics and vibration research group, Vrije Universiteit Brussel, Pleinlaan 2, Elsene, Belgium; E-Mails: bdepauw@vub.ac.be; allamber@vub.ac.be; julien.ertveldt@vub.ac.be; arezayat@vub.ac.be; . a lead-bismuth eutectic cooled installation and we apply modal analysis techniques to derive modal parameters of the system. We show that the .
1. Medical imaging coordinate naming 2. X-ray medical imaging Projected X-ray imaging Computed tomography (CT) with X-rays 3. Nuclear medical imaging 4. Magnetic resonance imaging (MRI) 5. (Ultrasound imaging covered in previous lecture) Slide 3: Medical imaging coordinates The anatomical terms of location Superior / inferior, left .
Medical X-Ray Medical Imaging N/A N/A 5-100 Tc-99m Medical Imaging (SPECT) 6.02 hours J 140.5 Tl-201 Medical Imaging (SPECT) 73 hours H 135, 167 In-111 Medical Imaging (SPECT) 2.83 days H 171, 245 F-18 Medical Imaging (PET) 1.83 hours E 511 Ga-68 Medical Imaging (PET) 68 minutes E 511 Cs-137 Fission Product 30.17 years E- 662
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BIBLIOGRAPHY Physics for Medical Imaging P. Allisy-Roberts, J. Williams – Farr’s Physics for Medical Imaging Radiological Physics P. Dendy, B. Heaton – Physics for Radiologists Medical Imaging J. Bushberg et al – The Essential Physics of Medical Imaging S. Webb – The Physics of Medical Imaging Nuclear Medicine
Rhode Island Medical Imaging: Greenwich Medical Center 1351 South County Trail, Suite 105, East Greenwich, RI 02818 : Rhode Island Medical Imaging: Blackstone Valley Medical Building 333 School Street, Suite 105, Pawtucket, RI 02860 . Rhode Island Medical Imaging: Moshassuck Medical Center 1 Randall Square, Suite 103, Providence, RI 02904
Plays are sometimes written from scratch; others are well known. KS3 Dramatic Society Club . of those students currently studying for ABRSM theory exams. Bring your lunch and have a chat. School Choir Large mixed (male and female voices) choir open to all years and abilities, which prepares for major musical events. Senior Jazz Combo Wild and unpredictable band of senior musicians in years .
