Analysis Of Alcohols In Hand Sanitizer And Impurities In Alcohol By GC .
AN-1051 Analysis of Alcohols in Hand Sanitizer and Impurities in Alcohol by GC-FID using Zebron ZB-624PLUS GC Columns Dr. Ramkumar Dhandapani, Zandra Baja, Zara Jalai, and Dr. Bryan Tackett Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA Figure 1. 50 ppm Acetaldehyde and 50 ppm methanol in 99.5% ethanol 3 Zebron ZB-624PLUS 30 meter x 0.32 mm x 1.80 µm 7HM-G040-31 Split 20:1 @ 200 C, 1 µL Zebron PLUS Z-Liner (Compatible with Agilent & Thermo GC instrument) AG2-0A03-05 Helium @ 25 cm/sec (Constant Flow) 36 C for 12 min, 260 C @ 10 C/min for 15 min FID 280 C Sample: 1. Acetaldehyde 2. Methanol 3. Ethanol App ID 26182 Overview GC-FID Conditions Alcohol-based hand sanitizers are used on a daily basis to Column: kill microorganisms including bacteria. Alcohol-based Dimension: sanitizers generally contains over 60% of alcohol. Part No.: However, if the alcohols are not at the right Injection: Recommended Liner: concentrations, it becomes ineffective. Thus, quantitative estimation of alcohol % in hand sanitizer via GC-FID is a Liner Part No.: very important analysis for identifying and quantifying the Carrier Gas: alcohols and content in hand sanitizer for both quality and Oven Program: branding purposes. In addition, the quality of alcohol used Detector: as raw material is equally important to avoid adverse Detector Temperature: effects. In this application note, we have developed a GCFID method for ascertaining the purity of raw material alcohol for impurity levels and quantitative method for % alcohol in hand sanitizer using a single method on a Zebron ZB-624PLUS GC Column. 1 2 Page 1 of 5 Have questions or want more details on implementing this method? We would love to help! Visit www.phenomenex.com/Chat to get in touch with one of our Technical Specialists
Figure 2. 100 ppm Acetaldehyde and 100 ppm methanol in 99.5% ethanol 3 Sample: 100 ppm each in Ethanol 1. Acetaldehyde 2. Methanol 3. Ethanol 1 App ID 26183 2 Figure 3. 200 ppm methanol in 99.5% ethanol 2 Sample: 200 ppm in Ethanol 1. Methanol 2. Ethanol App ID 26184 1 Page 2 of 3 Have questions or want more details on implementing this method? We would love to help! Visit www.phenomenex.com/Chat to get in touch with one of our Technical Specialists
Figure 4. USP impurities in 99.5% ethanol Sample: Impurities in Ethanol 1. 50 ppm Acetaldehyde 2. 50 ppm Methanol 3. Ethanol 4. 2 ppm Benzene 5. 30 ppm Acetal 6. 60 ppm 4-methylpentan-2-ol 6 3 4 Figure 5. Analysis of % content of Alcohol in Hand Sanitizer 1 App ID 26185 5 1 2 Sample: 31 mg/mL of Hand Sanitizer in DMF 1. Ethanol 2. Dimethylformamide - DMF (Diluent) App ID 26186 2 Page 3 of 5 Have questions or want more details on implementing this method? We would love to help! Visit www.phenomenex.com/Chat to get in touch with one of our Technical Specialists
Table 1. Precision Data for Ethanol Content in Hand Sanitizer on a Zebron ZB-624PLUS GC Column Injection # 1 2 3 4 5 6 % RSD Ethanol Retention Time (min) Peak Area 4.625 4.627 4.633 4.630 4.627 4.626 4719 4702 4650 4714 4633 4675 0.1% 0.8% Results and Discussion Due to increased demand for alcohol-based hand sanitizers, there is a need for a fast and efficient GC method to accurately quantify the % of alcohol in hand sanitizer. In addition, it is very important to make sure that the raw material alcohol used in the sanitizer manufacturing meets safety requirement for impurities. Presented in Figure 1 to 4 are the various impurities that are possible in alcohol raw material. The method for analysis utilizes a modified USP method that is still within allowable adjustments. The method provides identification and quantification of impurities that can be present in alcohol. The same method parameters were extended to the analysis of alcohol-based sanitizer. The sample alcohol sanitizer was dissolved in Dimethylformamide to quantify % alcohol content as shown in Figure 5. The precision of the method with respect to peak retention and peak area are presented in Table 1. In addition to analysis of alcohols, this method provides a way to analyse % alcohol content in hand sanitizer and to quantify impurities on a single method without the necessity to change method parameters and GC column. This is possible due to the optimal selectivity of ZB-624PLUS for volatile compounds like alcohols. In addition, the column has extensive crosslinkage through Engineered Self-Cross Linking (ESCTM) and thermal stability of 300/320 C max temperature to bake out contaminants. Conclusion Zebron ZB-624PLUS provides optimal selectivity and reproducibility for the analysis of alcohol and alcohol-based sanitizers in a single method. Page 4 of 5 Have questions or want more details on implementing this method? We would love to help! Visit www.phenomenex.com/Chat to get in touch with one of our Technical Specialists
AN40670721 W Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions. Trademarks Zebron, Engineered Self Cross-linking, ESC, 624PLUS, and BE-HAPPY are trademarks of Phenomenex. Agilent is a registered trademark of Agilent Technologies, Inc. Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. Disclaimer Phenomenex is in no way affiliated with Agilent Technologies, Inc, or Thermo Scientific. FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures. 2021 Phenomenex, Inc. All rights reserved. Page 5 of 5 Have questions or want more details on implementing this method? We would love to help! Visit www.phenomenex.com/Chat to get in touch with one of our Technical Specialists
ZebronZB-624 PLUS GC Column. Analysis of Alcohols in Hand Sanitizer and Impurities in Alcohol by GC-FID using Zebron ZB-624 PLUS GC Columns Dr. Ramkumar Dhandapani, Zandra Baja,Zara Jalai, and Dr. Bryan Tackett Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA Column: Dimension: Part No.: Injection: Recommended Liner: Liner Part No .
Chemistry Notes for class 12 Chapter 11 Alcohols, Phenols and Ethers Alcohols and Phenols Alcohols and phenols are formed when a hydrogen atom in hydrocarbon, aliphatic and aromatic respectively, is replaced by hydroxyl group (-OR group). Classification of Alcohols and Phenols In alcohols, -OR group is attached to Sp3 hybridised carbon.
Apr 12, 2009 · Section 3 - COMPOSITION / INFORMATION ON INGREDIENTS NAME CAS RN % alcohols C11-15 secondary ethoxylated 68131-40-8 98 ethylene oxide 75-21-8 trace C12-C15 alcohols i.e lauryl alcohols myristyl alcohols palmityl alcohols reacted with 6-9 mols of
Chapter 3 Alcohols, Phenols, and Ethers 2 3 Alcohols 4 The Hydroxy (—OH) Functional Group The hydroxyl group (—OH) is found in the alcohol and phenol functional groups. (Note: that’s not the same as hydroxide, OH-, which is ionic.) –in alcohols, a hydroxyl group is connected to a carbon atom. –in phenols, —OH is connected to a benzene ring. .
with thionyl chloride (SOCl 2) R-OH SOCl 2 R-Cl SO 2 HCl 3. Preparation of alkyl bromides by the treatment of alcohols with phosphorous tribromide (PBr 3) R-OH PBr 3 R-Br P(OH) 3 S N2 mechanism: works best with 1 and 2 alcohols, but not with 3 alcohols. stereochemistry: inversion. 50 98 OH (S)-2-hexanol O .
carbon-carbon bonds, a process that is termed nucleophilic addition. For example, Grignard reagents add to aldehydes to produce secondary alcohols, to ketones to produce tertiary alcohols, to carbon dioxide to form carboxylic acids, and to epoxides to form alcohols. Two moles of the reagent add to esters and acid chlorides to form .
Organic Chemistry Topic Notes 7 Alcohols A homologous series similar to alkanes but with a hydrogen replaced by an -OH (hydroxyl) group. The ending is -anol (eg: propanol). The general formula is CNH2N 1OH. There are primary, secondary and tertiary alcohols. Primary alcohols are when the ca
Test 1: Chromic Acid Oxidation This test distinguishes primary and secondary alcohols from tertiary. Chromic acid will oxidize a primary alcohol first to an aldehyde and then to a carboxylic acid and it will oxidize a secondary alcohol to a ketone. Tertiary alcohols do not react. The OH-bearing carbon must have a hydrogen atom attached. Recall,
Abrasive Jet machining can be employed for machining super alloys and refractory from materials. This process is based on surface erosion process. The process parameters that control metal removal rate are air quality and pressure, Abrasive grain size, nozzle material, nozzle diameter, stand of distance between nozzle tip and work surface. INTRODUCTION: Abrasives are costly but the abrasive .
