Gel Doc XR And ChemiDoc XRS Imaging Systems With
Gel Doc XR andChemiDoc XRS Imaging Systemswith Image Lab SoftwareInstrument GuideVersion 6.0
Gel Doc XR andChemiDoc XRS Imaging Systemswith Image Lab SoftwareInstrument GuideVersion 6.0
Bio-Rad Technical Support DepartmentThe Bio-Rad Technical Support department in the United States is open Monday through Friday,5:00 AM to 5:00 PM, Pacific time. Go to consult.bio-rad.com for worldwide technical support.Phone: 1-800-424-6723, option 2Web:consult.bio-rad.comEmail:Support@Bio-Rad.com (United States/Canada only)For technical assistance outside the United States and Canada, contact your local technical supportoffice.NoticeNo part of this publication may be reproduced or transmitted in any form or by any means, electronicor mechanical, including photocopy, recording, or any information storage or retrieval system,without permission in writing from Bio-Rad Laboratories, Inc.Bio-Rad reserves the right to modify its products and services at any time. This guide is subject tochange without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errorsor omissions, or for any damage resulting from the application or use of this information.Credits1.Image Lab software is based in part on the work of the Qwt project(http://qwt.sf.net).2.Image Lab software is based in part on the work of the CImg project(http://cimg.sourceforge.net/). See license for details atwww.cecill.info/licences/Licence CeCILL-C V1-en.html3.Image Lab software is based in part on the work of the Independent JPEG Group (www.ijg.org/).Alexa Fluor, Coomassie Fluor, Qdot, SYBR, and SYPRO are trademarks of Life TechnologiesCorporation. DyLight and Krypton are trademarks of Thermo Fisher Scientific Inc. GelGreen andGelRed are trademarks of Biotium, Inc. Slo-Blo is a trademark of Littelfuse. Mitsubishi is a trademarkof Mitsubishi Companies.Microsoft Office and Windows are registered trademarks of Microsoft Corporation. Mac OS is atrademark of Apple Inc.Bio-Rad Laboratories, Inc. is licensed by Life Technologies Corporation to sell SYPRO products forresearch use only under U.S. Patent Number 5,616,502. Bio-Rad Laboratories, Inc. is licensed byLife Technologies Corporation to sell reagents containing SYBR Green I for use in real-time PCR, forresearch purposes only.Criterion Stain Free gels are covered by U.S. Pat. No. 7,569,130. Bio-Rad Laboratories, Inc. islicensed by Invitrogen Corporation to sell SYPRO products for research use only under U.S. PatentNumber 5,616,502.Copyright 2017 Bio-Rad Laboratories, Inc. All rights reserved.
Table of ContentsSafety and Regulatory Compliance. . . . . . . . . . . . . . . . . . . . . . . . . . . .7Important Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7General Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Regulatory Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Alert Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Instrument Safety Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Notice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Power Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Voltage Setting Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Fuses. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13Gel Doc XR Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13ChemiDoc XRS Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14System Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14CCD Camera and Lenses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14Universal Hood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Image Lab Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Emission Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Optional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Conversion Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Instrument Guide iii
Table of ContentsChapter 2 Gel Doc XR Imager . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17Gel Doc XR Imager Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17Gel Doc XR Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Chapter 3 ChemiDoc XRS Imager . . . . . . . . . . . . . . . . . . . . . . . . . . . 21ChemiDoc XRS Imager Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21ChemiDoc XRS Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Chapter 4 Acquiring Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Creating Protocols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26Using Signal Accumulation Mode (SAM). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27Accessing Protocol Setup Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Choosing an Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29Configuring a Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30Configuring Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32Configuring Output Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32Positioning the Gel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33Running a Protocol with SAM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Saving SAM Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36Setting Up a Custom Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37Editing a Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39Chapter 5 System Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41Recalibrating the Imager . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42Appendix A Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45UV Transilluminator Lamp and Starter Replacement . . . . . . . . . . . . . . . . . . . . . . 45Epi-Illumination Lamp Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47Fuse Replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48iv Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
Table of ContentsAppendix B Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49Appendix C Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51Calibrating Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51Installing Optional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52UV/White Light Conversion Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52XcitaBlue Conversion Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54Gel Alignment Template Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57Orange Fluorescence Reference Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61Appendix D Using Bio-Rad Stain-Free Technology. . . . . . . . . . . . . 63Stain-Free Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64Electrophoresis with Stain-Free Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65Imaging Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65Imaging Blots. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67Instrument Guide v
Table of Contentsvi Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
Safety and Regulatory ComplianceImportant Safety InformationPlease read these instructions before attempting to operate the This instrument issuitable for research use only. It must be used, therefore, only by specializedpersonnel who know the health risks associated with the reagents that are normallyused with this instrument.WARNING! Use of the acrylic screen does not guarantee the user protectionfrom UV radiation. The use of protective eyeglasses, mask, and/or gloves isstrongly recommended.WarrantyThe is warranted against defects in materials and workmanship for one year. If anydefect occurs in the instrument during this warranty period, Bio-Rad Laboratories,Inc. will repair or replace the defective parts at its discretion without charge. Thefollowing defects, however, are specifically excluded: Defects caused by improper operation Repair or modification done by anyone other than Bio-Rad Laboratories,Inc. or the company’s authorized agent Use of spare parts supplied by anyone other than Bio-Rad Laboratories,Inc. Damage caused by accident or misuse Damage caused by disasterInstrument Guide 7
Safety and Regulatory Compliance Corrosion caused by improper solvents or samplesGeneral Precautions Read the user guide carefully. The instrument must be used only for the intended purpose of geldocumentation in research laboratories. The instrument must be connected to a grounded power source line andprotected by a circuit breaker. Do not pour liquids directly on or inside the instrument. Switch off all lights on the instrument immediately after use. Clean the transilluminator sample area after use.Regulatory NoticesThe Gel Doc XR or ChemiDoc XRS imaging system is designed and certified tomeet EN 61010, the internationally accepted electrical safety standard, EMCregulations, and TUV requirements. Certified products are safe to use whenoperated in accordance with this user guide. Do not modify or alter this instrumentin any way. Modification or alteration of this instrument will8 Void the manufacturer’s warranty Void the regulatory certifications Create a potential safety hazardGel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
Alert IconsAlert IconsAlert icons in this guide call attention to caution and warning paragraphs. Each iconindicates the type of hazard addressed.Table 1. How alert icons are used in this guideIconExplanationGeneralIndicates a potential hazard requiring special attention. This icon is usedwhen the hazard or condition is of a general nature.Electrical hazardIndicates a potential hazard requiring special attention when you are workingwith electricity or electrical equipment.Extreme heat and flammable materialsIndicates a potential hazard requiring special attention when you are workingwith extreme heat and flammable materials.Radiation hazardIndicates a potential hazard requiring special attention when you are workingwith UV radiation.CautionsA caution in this guide alerts you to take or avoid a specific action that could resultin loss of data or damage to the instrument. A caution can also indicate that, if theprecaution against a potential hazard is not taken, minor or moderate injury mightoccur.ExampleCaution: With the exception of cleaning or replacing light bulbs, refer allservicing to qualified Bio-Rad personnel or their agents.Instrument Guide 9
Safety and Regulatory ComplianceWarningsA warning in this guide precedes an action that, if not followed correctly, couldcause serious injury or death to the operator, serious or total loss of data, or seriousdamage to the instrument.ExampleWARNING! Use of the acrylic screen does not guarantee the user protectionfrom UV radiation. The use of protective eyeglasses, mask, and/or gloves isstrongly recommended.Instrument Safety WarningsBefore you operate the instrument, carefully read the contents of Table 2.Table 2. Safety cautions and warnings for the instrumentIconMeaningCaution: With the exception of cleaning or replacing light bulbs, refer allservicing to qualified Bio-Rad personnel or their agents. If youexperience technical difficulties with the instrument, contact Bio-Rad toschedule service. The instrument should not be modified or altered inany way. Alteration voids the manufacturer’s warranty and might createa potential safety hazard for the user.Caution: If the case interlock is defeated, there is a possibility of UV-Bradiation hazard due to UV-B light exposure. Exercise caution whenservicing the instrument.Caution: Disconnect the AC power cord before removing theinstrument cover.Warning: This instrument must be connected to an appropriate AC voltageoutlet that is properly grounded.10 Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
Power Safety InformationNoticeThe Gel Doc XR or ChemiDoc XRS imager is intended for laboratory use only.This device is meant for use by specialized personnel who know the health risksassociated with reagents normally used in electrophoresis. Bio-Rad Laboratories,Inc. is not responsible for any injury or damage caused by use of this instrument forpurposes other than those for which it is intended, or for instrument modificationsnot performed by Bio-Rad Laboratories, Inc. or an authorized agent.Power Safety InformationVoltage Setting InformationThe universal hood for the Gel Doc XR or ChemiDoc XRS imager has a powersupply that automatically chooses the correct voltage for your country or region.FusesThe universal hood for the Gel Doc XR or ChemiDoc XRS imager has two userserviceable fuses, F1 and F2, which are located on the bottom rear panel and are apart of the power entry module. See Fuse Replacement on page 48 for moreinformation.Instrument Guide 11
Safety and Regulatory Compliance12 Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
1IntroductionGel Doc XR and ChemiDoc XRS gel imaging systems contain a chargecoupled device camera (CCD) to capture images in real time. Image Lab softwarefacilitates accurate positioning of the sample and generates optimized image data.Each imager uses a lighttight enclosure (the universal hood) containing built-in UVand white light illumination. Both systems feature dynamic flat fielding technologyfor image uniformity and quantitation. Image Lab software controls image captureand optimization for the selected applications, analyzes results, and produces areport in a format you choose.This instrument guide describes the Gel Doc XR and ChemDoc XRS imagers andincludes general procedures for acquiring images. See the Image Lab SoftwareUser Guide for more detailed information about working with Image Lab software.Gel Doc XR Imaging SystemGel Doc XR imaging system is a high-resolution gel documentation system forquantitation of gels and blots. Position the sample inside the imager and follow theonscreen steps to run a protocol with one click. You can create custom applicationsfor a protocol or create a new protocol using many options in Image Lab software.Features include Detection of dynamic ranges that cover 3 orders of magnitude Dynamic flat fielding specific to fluorescent and colorimetric applicationsInstrument Guide 13
1 Introduction Smart, application-based protocol setup using Image Lab software, whichpresents appropriate filter and illumination sources for each application Ability to upgrade to the ChemiDoc XRS systemChemiDoc XRS Imaging SystemIn addition to the gel applications you can use with the Gel Doc XR imagingsystem, the ChemiDoc XRS imaging system offers sensitive chemiluminescentdetection. The imager includes a supersensitive CCD camera that is deeply cooledfor the long imaging times required for faint sample detection and image dataquantitation.Features include Suitability for imaging applications that require excellent sensitivity(chemiluminescent western blots) Smart, application-based protocol setup using Image Lab software, whichpresents appropriate filter and illumination sources for each application Dynamic range 4 orders of magnitude Flexibility to image chemiluminescent, fluorescent, and colorimetricsamples with dynamic flat fielding specific to each applicationSystem ComponentsCCD Camera and LensesThe CCD camera, equipped with a motorized zoom lens (MZL), sits on top of alighttight enclosure (the universal hood). After you perform initial calibration, apatented* algorithm controls the MZL to focus the lens automatically. Using ImageLab, you can control the MZL remotely to adjust zoom, focus, and iris.*U.S. patent 891312714 Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
System ComponentsFor camera specifications of each system, see the following: Gel Doc XR Technical Specifications on page 18 ChemiDoc XRS Technical Specifications on page 22Note: A factory installed 1 diopter lens makes the entire sample stage visible.This lens should always remain on the MZL assembly.Universal HoodThe universal hood captures fluorescent and chemiluminescent images withoutusing a photographic darkroom. The enclosure has built-in white light epiillumination and UV transillumination. For easy sample loading, the UVtransilluminator is located in the universal hood drawer and can be accessed fromthe front of the imager. When not imaging, the lights in the darkroom enclosure turnoff automatically.The universal hood has touchpad buttons to perform various functions, but you donot have to control the lens and lights manually. Instead, you can use Image Labsoftware to control all functions remotely. Running a protocol overrides touchpadinput.Image Lab SoftwareEach system includes Image Lab software. In addition to controlling the imagingsystems and acquiring and optimizing images, you can do the following in ImageLab: Annotate and document images Analyze molecular weights when imaging protein and nucleic acid gels Determine quantitation and purity of samplesYou can print all or a subset of data in a report. You can also export data to othersoftware, such as Microsoft Office , for further analysis or presentation options.See the Image Lab Software User Guide for detailed instructions.Instrument Guide 15
1 IntroductionEmission FiltersThe universal hood can hold two different emission filters for fluorescentapplications. No filter is required to image chemiluminescent samples.A standard filter (catalog #1708081) is used for colorimetric (white light) applicationsand is included in the installation kit.Optional AccessoriesBio-Rad Laboratories, Inc. offers a selection of optional filters and illuminationsources. See Appendix C, Accessories, for ordering information and a completelisting of accessory filters, UV light sources, optional parts, and replacement parts.PrinterFor your convenience, Bio-Rad offers an optional USB printer for use with theGel Doc XR and ChemiDoc XRS systems: the Mitsubishi thermal printer (catalog#1708089). For more information about the Mitsubishi thermal printer, see the ImageLab Software User Guide.Conversion ScreensWhite Light Conversion ScreenThe white light conversion screen (catalog #1708001) is a phosphor screen thatproduces white light transillumination when placed on top of the UV transilluminator.XcitaBlue Conversion ScreenThe optional XcitaBlue screen (catalog #1708182) is a UV to blue light conversionscreen, which enables you to visualize DNA samples while protecting them from UVdamage.16 Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
2Gel Doc XR ImagerRefer to the Gel Doc XR Installation Guide in the installation kit for informationabout installing and calibrating the imaging system. To recalibrate the imager whenyou acquire new accessories, refer to Chapter 5, System Calibration.Gel Doc XR Imager WorkflowFollowing are the basic steps for acquiring, analyzing, and archiving an image usingthe Gel Doc XR imager and Image Lab software:1.Select a protocol or create a new one.2.Position the sample you want to image.3.Run the protocol.4.View the results.5.Optimize the analysis.6.Generate a report.7.Save or export the results.Instrument Guide 17
2 Gel Doc XR ImagerGel Doc XR Technical escence*YesColorimetryYesGel documentationYesHardware SpecificationsMaximum sample sizeMaximum image areaExcitation sourceIllumination control Length: 28 cm Width: 36 cm Length: 19.4 cm Width: 26 cm Trans-UV and epi-white are standard (302 nmincluded, with 365 nm available as an option). Optional trans white, self-powered or conversionscreen. Optional XcitaBlue UV/blue conversion screen.3 modes: Trans-UV Trans white Epi-whiteDetectorCCDPixel size4.65 x 4.65 (H x V in microns)Cooling systemNot applicableCamera cooling temperatureNot availableFilter holder3 positions:Emission filters18 2 for emission filters 1 for using no filter 1 included (standard) 3 optional Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
Gel Doc XR Technical SpecificationsDynamic range 3.0 orders of magnitudePixel density (gray levels)4,096Dynamic flat fieldingApplication-specific, for all applicationsInstrument size Length: 36 cm Width: 60 cm Height: 96 cmInstrument weight32 kgOperating RangesOperating voltage110/115/230 V AC nominalOperating temperature10–28 C (21 C recommended)Operating humidity 70% noncondensingAutomation CapabilitiesWorkflow automated selectionApplication driven, user selected or recalled by aprotocolWorkflow automated executionControlled by a protocol via application-specificsetup for image area, illumination source, filter,analysis, and reportingWorkflow reproducibility100% repeatability via recallable protocols; fromimage capture to quantitative analysis and reportsAutofocusPrecalibrated focus for any zoom settingImage flat fieldingDynamic; precalibrated and optimized perapplicationAutoexposure2 user-defined modes (intense or faint bands)* When acquiring images from SYBR Safe DNA applications, Bio-Rad highly recommendsthat you use the optional XcitaBlue Conversion Screen kit (catalog #1708182), which enablesyou to visualize DNA samples and protects them against UV damage.Instrument Guide 19
2 20Gel Doc XR Imager Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
3ChemiDoc XRS ImagerRefer to the ChemiDoc XRS Installation Guide in the installation kit forinformation about assembling and calibrating the imaging system. To recalibrate theimager when you acquire new accessories, refer to Chapter 5, System Calibration.In addition to the applications that can be used with the Gel Doc XR , theChemiDoc XRS can acquire images from chemiluminescent blots.ChemiDoc XRS Imager WorkflowFollowing are the basic steps for acquiring, analyzing, and archiving an image usingthe ChemiDoc XRS system and Image Lab software:1.Select a protocol or create a new one.2.Position the sample to be imaged.3.Run the protocol.4.View the displayed results.5.Optimize the analysis.6.Generate a report.7.Save or export the results.When running chemiluminescent blots, you can use signal accumulation mode(SAM), which takes a series of exposures in a set period of time. You can thenchoose the image with the optimal signal. Refer to Chapter 3, Acquiring Images formore information.Instrument Guide 21
3 ChemiDoc XRS ImagerChemiDoc XRS Technical rescence*YesColorimetryYesGel documentationYesHardware SpecificationsMaximum sample sizeMaximum image areaExcitation sourceIllumination control Length: 28 cm Width: 36 cm Length: 26 cm Width: 35 cm Trans-UV and epi-white are standard (302 nm included, with365 nm available as an option) Optional trans white, self-powered or conversion screen Optional XcitaBlue UV/blue conversion screen5 modes: Trans-UV (standard) Epi-white (standard) No illumination for chemiluminescence (standard) Trans white (optional) XcitaBlue (optional)DetectorSupercooled CCDPixel size6.45 x 6.45 (H x V in microns)Cooling systemPeltier cooledCamera coolingtemperature–30 C controlledFilter selector3 positions:22 2 for filters 1 without filter (for chemiluminescence) Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
ChemiDoc XRS Technical SpecificationsEmission filters 1 included (standard) 3 optionalDynamic range 4.0 orders of magnitudePixel density (gray levels)65,535Dynamic flat fieldingApplication-specific, for all applicationsInstrument size Length: 36 cm Width: 60 cm Height: 96 cmInstrument weight32 kgOperating RangesOperating voltage110/115/230 V AC nominalOperating temperature10–28 C (21 C recommended)Operating humidity 70% noncondensingAutomation CapabilitiesWorkflow automatedselectionApplication driven, user selected or recalled by a protocolWorkflow automatedexecutionControlled by a protocol via application-specific setup for imagearea, illumination source, filter, analysis, and reportingWorkflow reproducibility100% repeatability via recallable protocols; from image captureto quantitative analysis and reportsAutofocusPrecalibrated focus for any zoom setting or sample heightImage flat fieldingDynamic; precalibrated and optimized per applicationAutoexposure2 user-defined modes (intense or faint bands)* When acquiring images from SYBR Safe DNA applications, Bio-Rad highly recommends that youuse the optional XcitaBlue Conversion Screen kit (catalog #1708182), which enables you to visualizeDNA samples and protects them against UV damage.Instrument Guide 23
3 ChemiDoc XRS Imager24 Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
4Acquiring ImagesImage Lab software runs configurable application-based, protocols for sampleimaging. In a single acquisition, a protocol runs a combination of settings foracquiring an image, analyzing it, and creating a customized report.Protocols can be retrieved, revised, and reused.Images you acquire at high resolution result in large files. For this reason, Bio-Radrecommends archiving images by exporting the files to network file server or toremovable storage media.For more detailed information about protocols, see the Image Lab Software UserGuide.Instrument Guide 25
4 Acquiring ImagesCreating ProtocolsCreating a protocol consists of configuring acquisition, analysis, and outputsettings. Analysis and output settings are optional.To create a protocol, you configure these settings on the Protocol Setup screens.The title bar above the Protocol Setup screen displays the protocol name andimager type. Main steps appear as headings in the left pane of each screen.Numbered steps appear under these headings.Analysis settings consist of the following: Detecting lanes and bands Analyzing molecular weightOutput settings consist of the following:26 Printing the image Printing the report Displaying the report Gel Doc XR and ChemiDoc XRS Imaging Systems with Image Lab Software
Creating ProtocolsTo choose options for a protocol step, select the checkbox to the left of thenumbered step. To disable a numbered step, clear its checkbox.Options for the selected step appear on the right side of the screen.To determine the optimum imaging time for a chemiluminescent sample, select thesignal accumulation mode (SAM) option.Using Signal Accumulation Mode (SAM)(ChemiDoc XRS only) When you run a chemiluminescence application, you canchoose signal accumulation mode (SAM) under Image Exposure on the Gel Imagingprotocol setup screen.SAM simplifies obtaining an optimal image from a chemiluminescent sample. Thissample type often requires long integration times to obtain an image that representsthe best range of signal. SAM acquires a number of images with different imagingtimes so you can compare the images and then select the optimum imaging time.SAM presents a series of cumulative images with progressively greater signal ineach image. To run SAM you must estimate the shortest and longest timesexpected to generate an image with the appropriate signal intensity. You thendecide how many total images to acquire in this period of time.For example, if the minimum expected time to image the sample is 1 minute and themaximum is 5 minutes, you enter these values (in
The universal hood for the Gel Doc XR or ChemiDoc XRS imager has a power supply that automatically chooses the correct voltage for your country or region. Fuses The universal hood for the Gel Doc XR or ChemiDoc XRS imager has two user-serviceable fuses, F1 and F2, which are located on the bottom rear panel and are a
The Bio-Rad Gel Doc 2000, ChemiDoc and the ChemiDoc XRS systems utilize a Universal Hood that is light tight, it contains UV and Epi-white light sources and all the power supplies needed to operate the Gel and ChemiDoc cameras. The ChemiDoc XRS c
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