INCUBATOR ACCESSORIES

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INCUBATOR ACCESSORIES:NUAIFE. INC.2100 FERNBROOK LANE. PLYMOUTH. MN 55447-4722(612)553-1370TOLL FPEE: [BOO] 328-3352FAX [6121553-0459NU-1550/NU-1550E AUTOFLOW AUTOMATIC TANK SWITCHOPERATION & INSTALLATION INSTRUCTIONSOPERATIONThe Autoflow Automatic Tank Switch has been designed to provide reliable service to performthe critical backup COj tank switch, for a tissue cell culture incubator. The Autoflow TankSwitch continuously monitors and indicates via the pilot light, the selected CO2 tank. Ifthepressure falls below 4 psig, the switch will occur from Tank 1 to Tank 2. During the initialpressure drop, a bu2zer will briefly tum on and the indicator light will display Tank 2. Thisindicates a switch has occurred and Tank 1 should be replaced.If for some reason. Tank 1 was not replaced and Tank 2 was depleted the switch would occurfirom Tank 2 to Tank 1 and remain on Tank 1 with the pilot light and bu22er on.The Autoflow Automatic Tank Switch installation is illustrated below. NOTE: The correctpositipn ofthe in-linefiltersand all tubing connections are fiimly in place. CD, OaHEICD TMB 2toss.CHjOET' Jr 7p cojuaaiCa,W3ICMM3CVrawc swncHINSTALLATION DIAGRAMIN0006 PG 1-4REV 2 5/96MJICfLCW TOaBMOR

DESCRIPTIDNITEM QTY3-WAY SDLENDID 115V)13-VAY SDLENDID (220V)LAMP GREEN (115V)LAMP GREEN (220V)LATCHING RELAY (120V)LATCHING RELAY (220V)PRESSURE SWITCHSWITCH (TOGGLE) DPDTTERMINAL BLOCKSWITCH SPST10BUZZER (120V)11BUZZER (230V)12LAMP RED (220V)13LAMP RED (115V)14POWER CORD (115V)15POWERCDRD (220V)16NUAIRE 999537A-999582REV ECODESCRIPTION5216 RELEASED TD PRDDUCTIDN LINE VOLTAGE115 VAC, 2 AMP, 50/60 HZ230 VAC. 1 AMP, 50/60 HZDATEINL INL2 / 2 3 / 9 6 TW X NIJAIREPLYMOUTH MINNESOTANU-1550 EXTERNALTANK SWITCHELECTRICAL SCHEMATICDFTM T W ] g / E 3 / 9 6SHEET 1Z ACD-0576ftDF 1A

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Tue 24 Aug '10 Nuaire RMA/solenoid replacement for ArnaudNuaire Inc / 2100 Fernbrook Ln N / Plymouth, MN 554471-800-328-3352http://www.nuaire.com/Model NU-1550( apparently a "C02 tank switch", similar to DH-C02 )( no information on the NU-1550 is available on-site )Mfg Date: 06142010Internal hoses burst, apparently as a result of overpressure(faulty regulator setting) during installation. This conditionalso appears to have damaged the 3-way solenoid usedto switch tanks.The solenoid is a Mac Valves ( http://www.macvalves.com )Model 35A-AAA-DAAA-1 BA 5.4 W 3-way valve, rated to120 psi. Mac valves: 1-800-622-8587 MC ouy nG. '

2100 Fembrook LaneTECHNICAL BULLETIN/ T \ACCESSORY INFORMATIONNfjjSlRt' / "' "''' " ''" ''"'nl APhone: 763.553.1270Fax: 763.553.0459Toll Free: 1.800.328.3352AUTOMATIC CO2 TANK SWITCH (INTERNAL)OPERATION AND INSTALLATION INSTRUCTIONSFOR NUAIRE INCUBATOR MODELS NU-5500/E/G (RF337)///OPERATIONTiie Automatic CO2 Tank Switch (intemal) has been designed to provide reliable service to perfonn thecritical backup CO2 tank switch for the DH Autoflow incubator. The Tank Switch continuouslymonitors the selected tank. Ifthe selected tank is Tank 1, the CO2 Tank 1 LED will hie on. Iftheselected tank is Tank 2, the CO2 Tank 2 LED will be on. The pressure switch point is 4 PSIG, so whenfirst tank is depleted and the pressure falls below 4 PSIG, the switch will occur from Tank 1 to Tank 2.At this time, the CO2 Tank 2 light is on as well as a continuous alarm and indicates a switch hasoccurred and Tank 1 should be replaced. Tum mode switch to set-up and back to run to clear theaudible alarm.— If for some reason, Tank 1 was not replaced and Tank 2 was depleted, the switch would occur fromTank 2 to Tank 1 and remain switching causing the CO2 Tank 2 light to blink until a new CO2 tank isinstalled.TOOLS REQUIRED:Phillips ScrewdriverSmall blade Screwdriver3/8" wrenchPliersPARTS INCLUDED IN C02 TANK SWITCH KIT:Tank Switch Valve Assembly (Solenoid Valve, Hose Barbs)Pressure Switch Assembly (Pressure Switch, Mounting Bracket)Tubing Assembly (tubing, 'T' Hose Barb)Misc.: hose clamps, screws, lahiel, tie wraps, bulk head fittingDrawings Included:BCD-06707BCD-06781NU-5500/E/G Electrical SchematicRear Panel AssemblyIf any questions arise during installation, please call NuAire Technical Service at 1-800-328-3352 or763-553-1270.ATB0091 PGl-8REV 2 9/05

2100 Fembrook LaneTECHNICAL BULLETINACCESSORY INFORMATION/ 9 \"'"' "*' '' '' " " " Phone: 763.553.1270Fax: 763.553.0459Toll Free: 1.800.328.3352AUTOMATIC CO2 TANK SWITCH (INTERNAL)OPERATION AND INSTALLATION INSTRUCTIONSFOR NUAIRE INCUBATOR MODELS NU-5500/E/G (RF337)OPERATIONThe Automatic CO2 Tank Switch (intemal) has been designed to provide reliable service to perform thecritical backup CO2 tank switch for the DH Autoflow incubator. The Tank Switch continuouslymonitors the selected tank. Ifthe selected tank is Tank 1, the 02 Tank 1 LED will be on. Iftheselected tank is Tank 2, the CO2 Tank 2 LED will be on. The pressure switch point is 4 PSIG, so whenfirst tank is depleted and the pressure falls below 4 PSIG, the switch will occur from Tank 1 to Tank 2.At this time, the CO2 Tank 2 light is on as well as a continuous alarm and indicates a switch hasoccurred and Tank 1 should be replaced. Tum mode switch to set-up and back to mn to clear theaudible alarm.If for some reason. Tank 1 was not replaced and Tank 2 was depleted, the switch would occur fromTank 2 to Tank 1 and remain switching causing the CO2 Tank 2 light to blink until a new CO2 tank isinstalled.TOOLS REQUIRED:Phillips ScrewdriverSmall blade Screwdriver3/8" wrenchPliersPARTS INCLUDED IN C02 TANK SWITCH KIT:Tank Switch Valve Assembly (Solenoid Valve, Hose Barbs)Pressure Switch Assembly (Pressure Switch, Mounting Bracket)Tubing Assembly (tubing, 'T' Hose Barb)Misc.: hose clamps, screws, label, tie wraps, bulk head fittingDrawings Included:BCD-06707BCD-06781NU-5500/E/G Electrical SchematicRear Panel AssemblyIf any questions arise during installation, please call NuAire Technical Service at 1-800-328-3352 or763-553-1270.ATB0091 PGl-8REV 2 9/05

HARDWARE ASSEMBLY PREPARATIONThere are two different mounting hole pattems on the back panel that require different hardwarepreparation and installation instructions. By looking at the back control panel ofthe unit, compare withenclosed drawings for (5) hole pattem, then follow appropriate instmction.DDol oi 3 0-0 CASE 1t c u r r i u i Hxc MTTTRHCASE 21. Tank Switch Valve Assembly Configuration:A right angle hose barb orientation (at port 1 of tank switch valve) needs to be adjusted dependingon which case. See figure below.CASE 2ATB0091REV 2 9/05

2. Pressure Switch Assembly:There are two sizes of pressure switch brackets. The bracket with the large flange is used for caseI, and the smaller flange is used for case 2. Depending on which case, use appropriate bracket.Assembly as shown.WASI-CRHCSe BARB (FEMALE) /13 PRESSURE SWITCH-VASKERBRACKE rThe Automatic Tank Switch intemal and extemal installation instmctions are illustrated by the followingdiagrams. NOTE: The correct position ofthe inline filters and all tubing and electrical connections arefirmly in place.EXTERNAL INSTALLATION DIAGRAMATB0091REV 2 9/05

INTERNAL COj TANK SWITCH INSTALLATION1. Remove black plastic hole plugs (5) on the back of rear panel.2. Install bulk head fitting on rear control panel as CO2 tank 2 port inlet (for case 2 only, see fig. 2 forlocation)3. Install CO2 tank switch valve assembly to the back ofthe rear panel using (2) existing holes andsecure it with screws provided (see appropriate drawing for location/orientation of valve assembly).4. Install Pressure switch assembly to the back of rear panel and secure it with screws provided (seeappropriate drawing for location/orientation of switch assembly).5. Locate CO2 inject valve on the back of rear panel. Disconnect tubing from CO2 tank 1 port to inlet(port # 1) of CO2 inject valve and connect to port #3 of CO2 tank switch valve.6. Attach tubing assembly as shown on figure 2.7. Locate CO2 tank switch valve, attach (2) black electrical leads to main control board P-7, position 1and 2.8. Locate pressure switch, attach electrical leads (yellow and black) to main control board P-5,position 1 and 2.9. Neatly tie down elecfrical wires using existing wire holders, and secure tubing with tie wrapsprovided.10. Connect CO2 supplies to unit (refer to O & M instmction for CO2 supply connecfion).11. Replace top control panel, plug in incubator, tum on power switch.COSTANK S U I T : HTJBINGCCN'IGJRATIDNCCASE 1)CM r « « 1 roit-C31 rA« iwrCH V.VCC32 TANK SWITCH TUBING CDSFIGLRATIDN;CASE 2)IWIT01 VM.VCCOI TfM« t K x r[ ] 00 . 3 .ATB0091REV 2 9/05nD

INTERNAL CO2 TANK SWTTCH INITUL SETUP1. In either RUN or SETUP mode, press and hold NUAIRE logo hidden key for 4 seconds to initiatediagnostic mode.2. Press ff to advance to "opt" menu, then press SEL key.3. Press SEL key to advance to CO2 tank 2 enable function. Temperature window displays "2.tn".This fimction will enable or disable the optional CO2 tank 2 system. The value display will show"ON" or "OFF" corresponding to the current condition.4. Press i\ to enable CO2 tank 2 option5. Press SEL key to advance to CO2 Default Tank. The temperature window displays "dF.t". Thisfimction will designate the default or primary CO2 tank to be used in the event the system isrestarted. This selection is normally made automatically during the tank switch operation.6. Press either fl or U to select default tank.7. Press NUAIRE hidden key twice to exit diagnostic mode.Note: Refer to NU-5500 Operation Manual, Section 8.8.1.2 for detailed information on intemal CC)2tank switch setting-up and testing.ATB0091REV 2 9/05

WarrantyNuAire, Inc., warrants that it will repair F.O.B. its factory or fiimish without charge F.O.B. its factory,a similar part to replace any material in its equipment within 12 months after the date of sale if proved tothe satisfaction ofthe company to have been defective at the time it was sold provided that all partsclaimed defective shall be retumed, properly identified to the company at its factory, charges prepaid.Factory installed equipment or accessories are warranted only to the extent guaranteed by the originalmanufacturer, and this warranty shall not apply to any portion ofthe equipment modified by the user.Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of thedefect, the date ofthe initial installation and the serial and model number ofthe equipment.This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse,abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts ofGod. Ifthe serial number ofthis product is altered, removed or defaced as to be illegible, the warrantyshall be null and void in its entirety.The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Priorto returning any item, for any reason, contact NuAire, Inc. for a Retum Authorization Number. Thisnumber must accompany all retums. Any product shipped to NuAire without this number will beretumed, refused shipment or collect freight.ShipmentNuAire takes every reasonable precaution to insure that your incubator arrives without damage. Motorcarriers are carefully selected and shipping cartons have been specially designed to insure yourpurchase. However, damage can occur in any shipment and the following outlines the steps you shouldtake on receipt of a NuAire incubator to be sure that if damage has occurred, the proper claims andactions are taken immediately.Damaged ShipmentsTerms are factory, unless stated otherwise. Therefore, it is important to check each shipment beforeacceptance.If there is visible damage, the material can be accepted after the driver makes a notation on theconsignee's copy of the freight bill. Then an inspection must be made to verify the claim against thecarrier. This inspection is the basis of your filing the claim against the carrier.If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT ATONCE, and request an inspection. Without this inspection, the transportation company may not accepta claim for loss or damage. If the carrier will not perform the inspection, an affidavit must be preparedstating the he was contacted on a certain date and that he/she failed to comply with the request. Thisalong with other papers in the customer's possession will support the claim.ATB0091REV 2 9/05

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HARDWARE ASSEMBLY PREPARATIONThere are two different mounting hole pattems on the back panel that require different hardwarepreparation and installation instmctions. By looking at the back control panel of the unit, compare withenclosed drawings for (5) hole pattem, then follow appropriate instmction./ nDF""" IV". iiTic ri.-iUJ- '" DIJ'M;Lp-tcugTjic MXt r -Ttai.L"! .rEQ"I. Tank Switch Valve Assembly Configuration:A right angle hose barb orientation (at port 1 of tank switch valve) needs to be adjusted dependingon which case. See figure below.CASE 2ATB0091REV 2 9/05

2. Pressure Switch Assembly:There are two sizes of pressure switch brackets. The bracket with the large flange is used for case1, and the smaller flange is used for case 2. Depending on which case, use appropriate bracket.Assembly as shown.V.-iSlC? - ,/'v[fOtr.:i-'.iLt;ipmSiy / '. - pi r:i;-:i sr ;» !!:: .'ASKLi Hk:- CKL1The Automatic Tank Switch intemal and extemal installation instmctions are illustt-ated by the followingdiagrams. NOTE: The correct position ofthe inline filters and all tubing and electrical connections arefirmly in place.EXTERNAL INSTALLATION DIAGRAMc 9 IMK t t r ic a lAJM ] » a i/Figure 1ATB0091REV 2 9/05

INTERNAL CQ2 TANK SWITCH INSTALLATIONRemove black plastic hole plugs (5) on the back of rear panel.Install bulk head fitting on rear control panel as CO2 tank 2 port inlet (for case 2 only, seefig.2 forlocation)Install CO2 tank switch valve assembly to the back ofthe rear panel using (2) existing holes andsecure it with screws provided (see appropriate drawing for location/orientation of valve assembly).Install Pressure switch assembly to the back of rear panel and secure it with screws provided (seeappropriate drawing for location/orientation of switch assembly).5.Locate CO2 inject valve on the back of rear panel. Disconnect tubing from CO2 tank 1 port to inlet(port #1) of CO2 inject valve and connect to port #3 of CO2 tank switch valve.6. Attach tubing assembly as shown on figure 2.7. Locate CO2 tank switch valve, attach (2) black electrical leads to main control board P-7, position 1and 2.Locate pressure switch, attach electrical leads (yellow and black) to main control board P-5,position 1 and 2.Neatly tie down electrical wires using existing wire holders, and secure tubing with tie wrapsprovided.10, Connect CO2 supplies to unit (refer to O & M instmction for CO2 supply connection).11, Replace top control panel, plug in incubator, tum on power switch.CDS TANKSWIT:H TUBING(CASECDNriQJRATinN1)CR IM« 1 .Wln t I.JECT VM,VtIJM( IVITCH VN.VICG2 TA.NK SVITCH TUBING(CASE 2)CD\F:GURAT!DNlAJH IvnCNUWIWJ. NM.rt.ATB0091REV 2 9/05( tVITOI VM.VC

INTERNAL CO2 TANK SWITCH INITIAL SETUP1. In either RUN or SETUP mode, press and hold NUAIRE logo hidden key for 4 seconds to initiatediagnostic mode.2. Press fl to advance to "opt" menu, then press SEL key.3. Press SEL key to advance to CO2 tank 2 enable function. Temperature window displays "2.m".This fimction will enable or disable the optional CO2 tank 2 system. The value display will show"ON" or "OFF" corresponding to the current condition.4. Press ff to enable CO2 tank 2 option5. Press SEL key to advance to CO2 Default Tank. The temperature window displays "dF.t". Thisfunction will designate the default or primary CO2 tank to be used in the event the system isrestarted. This selection is normally made automatically during the tank switch operation.6. Press either fl or U to select default tank.7. Press NUAIRE hidden key twice to exit diagnostic mode.Note: Refer to NU-5500 Operation Manual, Section 8.8.1.2 for detailed information on intemal CQ2tank switch setting-up and testing.ATB0091REV 2 9/05

WarrantyNuAire, Inc., warrants that it will repair F.O.B. its factory or fumish without charge F.O.B. its factory,a similar part to replace any material in its equipment within 12 months after the date of sale if proved tothe satisfaction ofthe company to havebeen defective at the time it was sold provided that all partsclaimed defective shall be retumed, properly identified to the company at its factory, charges prepaid.Factory installed equipment or accessories are warranted only to the extent guaranteed by the originalmanufacturer, and this warranty shall not apply to any portion ofthe equipment modified by the user.Claims under this warranty should be directed to NuAire, Inc. setting forth in detail the nature of thedefect, the date of the initial installation and the serial and model number of the equipment.This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse,abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts ofGod. Ifthe serial number ofthis product is altered, removed or defaced as to be illegible, the warrantyshall be null and void in its entirety.The warranty is for the sole benefit ofthe original purchaser and is not assignable or transferable. Priorto retuming any item, for any reason, contact NuAire, Inc. for a Retum Authorization Number. Thisnumber must accompany all retums. Any product shipped to NuAire without this number will beretumed, refused shipment or collect freight.ShipmentNuAire takes every reasonable precaution to insure that your incubator arrives without damage. Motorcarriers are carefully selected and shipping cartons have been specially designed to insure yourpurchase. However, damage can occur in any shipment and the following outlines the steps you shouldtake on receipt of a NuAire incubator to be sure that if damage has occurred, the proper claims andactions are taken immediately.Damaged ShipmentsTerms are factory, unless stated otherwise. Therefore, it is important to check each shipment beforeacceptance.If there is visible damage, the material can be accepted after the driver makes a notation on theconsignee's copy ofthe freight bill. Then an inspection must be made to verify the claim against thecarrier. This inspection is the basis of your filing the claim against the carrier.If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT ATONCE, and request an inspection. Without this inspection, the transportation company may not accepta claim for loss or damage. Ifthe carrier will not perform the inspection, an affidavit must be preparedstating the he was contacted on a certain date and that he/she failed to comply with the request. Thisalong with other papers in the customer's possession will support the claim.ATB0091REV 2 9/05

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5. Locate CO2 inject valve on the back of rear panel. Disconnect tubing from CO2 tank 1 port to inlet (port # 1) of CO2 inject valve and connect to port #3 of CO2 tank switch valve. 6. Attach tubing assembly as shown on figure 2. 7. Locate CO2 tank switch valve, attach (2) black electrical leads to main

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