SOP D2 Phaser XRD - Chemistry

LUMIGEN INSTRUMENT CENTER X-RAY CRYSTALLOGRAPHYICLABORATORY: WAYNE STATE UNIVERSITYStandard Operating Procedure for the Bruker D2 Phaser X-RayDiffractometerContact Manager:Dr. Cassie Wardward@wayne.eduOffice room 061 Chemistry(313) 577-2587Dr. Sameera Pererafp4261@wayne.eduOffice room 135 ChemistryLocationLumigen Instrument Center X-Ray Crystallographic LaboratoryDepartment of ChemistryWayne State University 050.1 Chemistry Building5101 Cass AvenueDetroit, MI 48202LIC Lab: (313) 577-0518Safety Requirements for the X-ray Crystallographic LaboratoryAccess to the lab will be revoked if you do not follow these safety procedures.1. While working in the X-Ray Crystallographic Laboratory, researchers are alwaysrequired to wear Personal Protective Equipment (PPE). The appropriate PPE includedosimetry ring, safety glasses, long pants/skirt covering the legs completely, closed-toeshoes, and gloves. Do not wear gloves while using the computer keyboard or mouse. Allrequired PPE needs to be supplied by the user. Dosimetry rings must not be removedfrom the lab.2. Food and beverages cannot enter the lab. Never eat or drink inside the lab.3. Please keep the area around the instruments and prep stations clean. If you use the lab’ssample holders, clean them after use. Properly dispose glass waste in the appropriatecontainer. All samples must be properly disposed of in your research lab.4. Following a spill, please clean it up with a paper towel or broom. If you are unsure howto clean up the spill, contact the lab manager (Dr. Ward) or the LIC Director (Dr.Westrick). If the spill occurs after hours with personal injury, please contact WSU policedepartment (7-2222) and Dr. Westrick, immediately. If samples come in contact with theeyes, use the emergency eye wash station. Turn on the cold water on high and pull theknob to the eye wash. Run water to the eyes for 15 minutes.5. The Standard Operating Procedures (SOPs) are next to the instruments. The users are tofollow what is written in these procedures.6. You must log the usage time into the appropriate instrument logbook.7. All researchers working in LIC must complete the EH&S initial course for LaboratorySafety Training and Radiation (X-ray) Generating Machine training and show proof ofcompletion. Users whose safety training has expired will not be permitted access to thelaboratory.8. Users must also sign the Wayne State University Laboratory Specific Training Form(Appendix L).9. In case of an instrument malfunction, turn off the instrument and contact Dr. WardWardUpdated: 2020 March 6Page 1 of 8

LUMIGEN INSTRUMENT CENTER X-RAY CRYSTALLOGRAPHYICLABORATORY: WAYNE STATE UNIVERSITYimmediately.10. Be aware that the X-Ray Crystallography Laboratory has a red binder containing theChemical and Laboratory Safety Information. It is located on the second shelf above thedosimetry rings cabinet. This contains the Department of Licensing and RegulatoryAffairs Radiation Safety Section Ionizing Radiation Rules.Additional Safety Requirements for the D2 Phaser powder XRD1. In case of an instrument malfunction, turn off the instrument using the switch in the back(Figure 1). Contact Dr. Ward immediately, or the WSU police (313-577-2222) ifoccurrence is after hours.Figure 1: For emergency shut down, turn off the switch in the back of the D2 Phaser as shown inthe picture.Training and Usage RequirementsBefore Training:1. All users must pass the online EH&S Laboratory Safety Training and Radiation (X-ray)Generating Machine training (https://about.citiprogram.org/en/homepage/). Print thecertificate after completing the quiz and bring it to Dr. Ward. The two safety trainingsmust be completed every Janurary and the printed certificate must be brought to Dr.Ward.2. A dosimetry form must also be filled out st-form.pdf) and signed by Dr. Westrick. Bring the signed form to Dr. Ward.3. You must have an Infinity account and have requested access to the Lumigen X-rayLaboratory. You must also have

Standard Operating Procedure for the Bruker D2 Phaser X-Ray Diffractometer Contact Manager: Dr. Cassie Ward Dr. Sameera Perera ward@wayne.edu fp4261@wayne.edu Office room 061 Ch